Precise sample adding system, in-vitro diagnosis equipment and precise sample adding method
阅读说明:本技术 一种精密加样系统、体外诊断设备及精密加样方法 (Precise sample adding system, in-vitro diagnosis equipment and precise sample adding method ) 是由 秦正贵 赵松涛 刘展宏 陈力勇 赵明宇 于 2020-07-09 设计创作,主要内容包括:本发明涉及一种精密加样系统、体外诊断设备及精密加样方法,所述精密加样系统,包括:用于加热去离子水和除去去离子水中气泡的恒温除气机构;与恒温除气机构连接用于控制去离子水供给的第一控制阀;与第一控制阀连接用于定量供给去离子水的第一柱塞泵;与第一柱塞泵连接用于控制管路的通断和切换的管路控制机构;与管路控制机构连接用于定量供给样本液的第二柱塞泵;与第二柱塞泵连接用于吸取和吐出样本液的样本针。本发明所述精密加样系统,具有独特的管路连接配置方式,能够为样本液提供一段辅助去离子水水柱,使样本液精确完全吐出,同时采用恒温除气机构脱除了去离子水中的气泡,从而能够保障柱塞泵挤压液体滴样的过程精度更高。(The invention relates to a precise sample adding system, in-vitro diagnostic equipment and a precise sample adding method, wherein the precise sample adding system comprises: a constant temperature degassing mechanism for heating the deionized water and removing bubbles in the deionized water; the first control valve is connected with the constant-temperature degassing mechanism and used for controlling the supply of the deionized water; the first plunger pump is connected with the first control valve and used for quantitatively supplying deionized water; the pipeline control mechanism is connected with the first plunger pump and is used for controlling the on-off and switching of the pipeline; a second plunger pump connected with the pipeline control mechanism and used for quantitatively supplying the sample liquid; and a sample needle connected to the second plunger pump for sucking and discharging a sample liquid. The precise sample adding system has a unique pipeline connection configuration mode, can provide a section of auxiliary deionized water column for sample liquid, enables the sample liquid to be precisely and completely discharged, and simultaneously adopts a constant-temperature degassing mechanism to remove bubbles in the deionized water, so that the process precision of extruding liquid drop samples by the plunger pump can be ensured to be higher.)
1. A precision sample application system, comprising:
a constant temperature degassing mechanism for heating the deionized water and removing bubbles in the deionized water;
the first control valve is connected with the constant-temperature degassing mechanism and is used for controlling the supply of the deionized water;
the first plunger pump is connected with the first control valve and used for quantitatively supplying deionized water;
the pipeline control mechanism is connected with the first plunger pump and is used for controlling the on-off and switching of a pipeline;
a second plunger pump connected to the pipe control mechanism for quantitatively supplying the sample liquid;
and a sample needle connected with the second plunger pump and used for sucking and spitting out sample liquid.
2. The precision sample addition system of claim 1, further comprising: and the cleaning needle is connected with the pipeline control mechanism and is used for spitting the deionized water supplied by the first plunger pump.
3. The precision sample addition system according to claim 2, wherein the conduit control mechanism comprises: the cleaning device comprises a three-way control valve and a second control valve, wherein the three-way control valve is arranged between the first plunger pump and the second plunger pump and used for switching different pipelines, and the second control valve is arranged between the three-way control valve and the cleaning needle and used for controlling the on-off of the pipelines.
4. The precise sample adding system according to claim 3, wherein a bubble optical coupler for detecting the bubble removal effect in the deionized water is further arranged between the first plunger pump and the three-way control valve.
5. The precise sample adding system of claim 1, wherein a pressure sensor for monitoring the pressure of the liquid path of the precise sample adding system in real time is further arranged between the first control valve and the first plunger pump.
6. The precision sample addition system of claim 1, wherein the displacement of the second plunger pump is less than the displacement of the first plunger pump.
7. The precision sample addition system according to claim 1, wherein the constant temperature degassing mechanism comprises: the spiral rising pipeline is used for deionized water to pass through, and the heating device is used for heating the deionized water flowing through the spiral rising pipeline; and a temperature detection device for detecting the temperature of the deionized water.
8. The precision sample addition system according to claim 7, wherein the temperature detection device comprises at least one temperature sensor.
9. An in vitro diagnostic device comprising a precision sample application system according to any one of claims 1 to 8.
10. A precise sample adding method based on the precise sample adding system of any one of claims 1 to 8, which comprises the following steps:
A. opening a first control valve, and controlling a pipeline control mechanism to close a pipeline between the first plunger pump and the second plunger pump;
B. starting a first plunger pump to operate and absorb deionized water, simultaneously starting a constant-temperature degassing mechanism to heat the deionized water and remove bubbles in the deionized water, and closing the first plunger pump and a first control valve after the water amount in the first plunger pump is stored to a set value;
C. controlling a pipeline control mechanism to enable a pipeline between the first plunger pump and the second plunger pump to be communicated, starting the second plunger pump to run, and quantitatively sucking sample liquid through the sample needle;
D. and controlling the second plunger pump to spit the sample liquid, wherein in the process of spitting the sample liquid, a pipeline of the precise sample adding system provides a section of auxiliary deionized water column for removing bubbles for the sample liquid, so that the sample liquid is precisely and completely spit.
Technical Field
The invention relates to the field of in-vitro diagnosis equipment, in particular to a precise sample adding system, in-vitro diagnosis equipment with the precise sample adding system and a precise sample adding method based on the precise sample adding system.
Background
With the increasing demand of clinical diagnosis in China and the increasing development of research and development technologies, the In Vitro Diagnosis (IVD) industry has become the fastest-developing and one of the most active industries in the pharmaceutical industry in China. Although the national In Vitro Diagnosis (IVD) industry still has a certain gap compared with the developed national industry, the rapid development and the huge potential of the IVD industry are continuously paid attention and supported by the nation.
In vitro diagnosis is the in vitro detection of human body samples (various body fluids, cells, tissue samples, etc.) to achieve the effects of disease prevention, diagnosis, treatment monitoring, prognosis observation, health status evaluation and genetic disease prediction. However, the in-vitro diagnostic apparatus inevitably has a large deviation of the detection result, even serious problems such as false detection and false detection in the process of analyzing the data of the human body sample. The method for avoiding the problems reduces the external factors such as manual misoperation, and the like, and the method is started from the equipment. There may be many that affect the accuracy of the in vitro diagnostic device, for example: inaccurate adding amounts of samples and reagents provided for equipment detection lead to inaccurate test results caused by inaccurate reaction liquid; the vessels for reaction and observation cannot be cleaned completely, and reaction liquid pollution caused by cross contamination of different samples after multiple detections influences the test result; the device test flow is unreasonable, and result deviation is caused; reagents, sample deterioration, etc.
The biochemical analyzer is one kind of in vitro diagnosis equipment, and belongs to the field of optical analysis instrument. The monochromator divides polychromatic light emitted by the light source into monochromatic light, the monochromatic light with specific wavelength passes through a cuvette filled with a sample solution, and the photoelectric converter converts transmitted light into an electric signal and then sends the electric signal to the signal processing system for analysis. Continuous optical analysis requires the cooperation of a continuous sample filling system with a continuous cuvette washing system and other related systems. However, in the continuous sample filling and cleaning processes, small differences in the sample dropping process can cause inaccurate sample filling amount, and sample cross contamination is easily caused by incomplete cleaning of the sample due to poor cleaning effect in the repeated cleaning processes, so that the final detection result has deviation.
Accordingly, the prior art is yet to be improved and developed.
Disclosure of Invention
In view of the above-mentioned shortcomings of the prior art, the present invention is directed to a precise sample application system, an in vitro diagnostic device and a precise sample application method, so as to overcome the problem of deviation of the final detection result caused by small difference in the sample application process and poor cleaning effect in the repeated cleaning process in the prior art.
The technical scheme of the invention is as follows:
a precision sample addition system comprising: a constant temperature degassing mechanism for heating the deionized water and removing bubbles in the deionized water; the first control valve is connected with the constant-temperature degassing mechanism and is used for controlling the supply of the deionized water; the first plunger pump is connected with the first control valve and used for quantitatively supplying deionized water; the pipeline control mechanism is connected with the first plunger pump and is used for controlling the on-off and switching of a pipeline; a second plunger pump connected to the pipe control mechanism for quantitatively supplying the sample liquid; and a sample needle connected with the second plunger pump and used for sucking and spitting out sample liquid.
An in vitro diagnostic device comprising a precision sample application system as described above.
A precise sample adding method based on the precise sample adding system comprises the following steps:
A. opening a first control valve, and controlling a pipeline control mechanism to close a pipeline between the first plunger pump and the second plunger pump;
B. starting a first plunger pump to operate and absorb deionized water, simultaneously starting a constant-temperature degassing mechanism to heat the deionized water and remove bubbles in the deionized water, and closing the first plunger pump and a first control valve after the water amount in the first plunger pump is stored to a set value;
C. controlling a pipeline control mechanism to enable a pipeline between the first plunger pump and the second plunger pump to be communicated, starting the second plunger pump to run, and quantitatively sucking sample liquid through the sample needle;
D. and controlling the second plunger pump to spit the sample liquid, wherein in the process of spitting the sample liquid, a pipeline of the precise sample adding system provides a section of auxiliary deionized water column for removing bubbles for the sample liquid, so that the sample liquid is precisely and completely spit.
The invention has the beneficial effects that: the precise sample adding system has a unique pipeline connection configuration mode, can provide a section of auxiliary deionized water column for the sample liquid in the process of spitting the sample liquid, thereby eliminating the difference caused by dead volume and repeated movement in the plunger pump, and ensuring that the sample liquid is precisely and completely spitted.
Drawings
FIG. 1 is a schematic structural diagram of a preferred embodiment of a precise sample application system according to the present invention;
FIG. 2 is a schematic view of a partial structure of a preferred embodiment of the precise sample application system according to the present invention;
FIG. 3 is another schematic partial structure diagram of a precise sample application system according to a preferred embodiment of the present invention.
Detailed Description
The present invention provides a precise sample adding system, an in vitro diagnostic device and a precise sample adding method, and in order to make the purpose, technical scheme and effect of the present invention more clear and definite, the present invention is further described in detail below with reference to the attached drawings and examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The precise sample adding system of the preferred embodiment of the present invention, as shown in fig. 1, includes: a constant temperature degassing mechanism 1 for heating deionized water and removing bubbles in the deionized water; a
Further, as shown in fig. 1 and fig. 3, the precision sample adding system further includes: a
Furthermore, the
Further, as shown in fig. 2 and 3, the pipeline control mechanism 4 specifically includes: a three-way control valve 41 arranged between the
Further, the displacement of the
Preferably, the three-way control valve 41 is a three-way electromagnetic valve, and controls on-off switching between the pipelines from the
Preferably, as shown in fig. 1 to 3, in an embodiment of the present invention, a bubble optical coupler 8 for detecting a bubble removing effect in deionized water is further disposed between the
Preferably, as shown in fig. 1 to fig. 3, in the embodiment of the present invention, a pressure sensor 9 for monitoring the pressure of the liquid path of the precise sample adding system in real time is further disposed between the
Specifically, in the sucking and spitting process of the first plunger pump 3 (large-displacement plunger pump), the pressure sensor 9 detects the change of the internal pressure value of the system pipeline, and when the blockage of devices, pipelines and an output end occurs in the circulation process of the liquid path, the internal pressure of the system is increased due to the change of the drift diameter to give an alarm, so that the system device is convenient to inspect, maintain and maintain. Except that reporting to the police under the inside condition that has the jam of system, when big discharge capacity plunger pump absorbs deionized water, because the control valve on-off state is incomplete, cause the pressure release and finally influence under the big discharge capacity plunger pump water absorption not enough condition, pressure sensor 9 compares the pressure range under the normal condition, can play the detection guard action equally.
In the embodiment of the invention, the precise sample adding system mainly comprises two parts, wherein one part is a part for supplying deionized water to the system, and the final output end of the system is a cleaning needle, as shown in fig. 1 and 3; the other part is a sample liquid sucking and discharging part of the system, the part is communicated with a water supply part, deionized water can be selectively supplied to the part through a three-way control valve 41, and the final output end of the part is a sample needle as shown in fig. 1 and 2. Specifically, referring to fig. 1 to 3 together, in the embodiment of the present invention, deionized water introduced through an external pipeline is heated and degassed by the constant temperature degassing mechanism 1, and then flows through: the
Further, as shown in fig. 2 and 3, in the embodiment of the present invention, the constant temperature degassing mechanism 1 specifically includes: a spiral rising pipe 11 for passing deionized water, and a heating device (not shown in the figure) for heating the deionized water flowing through the spiral rising pipe 11; and a temperature detecting device (not shown) for detecting the temperature of the deionized water. The heating means may be provided on the outer wall of the spiral rising conduit, the heating means preferably being an electrical heating means, such as a heating jacket; and the temperature detection device comprises at least one temperature sensor, and the temperature sensor can be tightly attached to the outer wall of the spiral rising pipeline 11 to monitor the temperature of the deionized water passing through the spiral rising pipeline 11 in real time.
Constant temperature degasification mechanism 1 provides the heating environment for exporting deionized water, gets rid of the subassembly device of aquatic bubble simultaneously. On the one hand, constant temperature degasification mechanism is equipped with heating and temperature-detecting device, makes the deionized water that flows through and out satisfy an anticipated temperature range of setting for through control, under appropriate temperature, remains the sample liquid on wasing needle inner wall and reaction cup outer wall and wash more easily and drop to promote the cleaning performance. On the other hand, the inner structure of constant temperature degasification mechanism is the space of form of spiraling, under the condition of giving certain vacuum pressure inside, through tubular product (withstand voltage pipe) and the pressure fit of special material, can make a large amount of bubbles that melt into in the inside deionized water of pipeline break away from and go out, the deionized water after the processing because degasification's the reason for the volume change volume is littleer in compression process after, thereby can ensure that the process precision of plunger pump extrusion liquid drop appearance is higher.
More specifically, the external deionized water supply device supplies deionized water to the constant temperature degassing mechanism 1, the deionized water enters the constant temperature degassing mechanism 1 from the lower end of the constant temperature degassing mechanism 1, a heating device with certain power is arranged in the constant temperature degassing mechanism 1, and heat generated by consuming electric energy is transferred to the deionized water flowing through the heating device, so that the effect of supplying water for heating is achieved. By setting heating power, adjusting liquid flow rate, calculating and counting heat loss in the flowing process and other factors, the temperature of the deionized water at the output end suitable for equipment is finally obtained, for example, 40 ℃, and the heated deionized water has a better cleaning effect than normal-temperature deionized water. The constant temperature degassing mechanism has the function of heating and the effect of removing gas. The pump type that can provide the negative pressure through the pipe connection for the inside negative pressure environment that has certain adjustable range of constant temperature degasification mechanism can make the bubble of the deionized water under the negative pressure state of flowing through peel off and go out under the tubular product cooperation of selecting suitable material, then reaches degassed effect. The constant-temperature degassing mechanism 1 has a sealed space which spirally rises and passes through, and the pipeline is spirally arranged in the sealed space.
The working principle of the precise sample adding system in one embodiment of the invention is as follows:
the constant temperature degassing mechanism 1 → the
When a sample liquid container such as a cuvette needs to be cleaned, a motor drives a first plunger pump 3 (a large-displacement plunger pump) to suck constant-temperature degassed deionized water into a plunger pump cavity, at the moment, a
When the sample liquid needs to be added, the control three-way control valve 41 is switched to be communicated with the second plunger pump 5 (small displacement plunger pump), and the second control valve 42 is in a closed state. The small-displacement plunger pump motor drives the plunger to move, so that the internal pressure is a negative pressure value, and then liquid is sucked from the outside and enters the cavity of the plunger pump. In order to suck the sample into the cavity of the sample needle and the cavity inner space of the connecting pipeline, the
The embodiment of the invention also provides a precise sample adding method based on the precise sample adding system, which comprises the following steps:
s100, opening a first control valve, and controlling a pipeline control mechanism to close a pipeline between the first plunger pump and the second plunger pump;
specifically, the
S200, starting a first plunger pump to operate and absorb deionized water, simultaneously starting a constant-temperature degassing mechanism to heat the deionized water and remove bubbles in the deionized water, and closing the first plunger pump and a first control valve after the water amount in the first plunger pump is stored to a set value.
S300, controlling a pipeline control mechanism to enable a pipeline between the first plunger pump and the second plunger pump to be communicated, starting the second plunger pump to run, and quantitatively sucking sample liquid through a sample needle;
specifically, the method comprises the following steps: the three-way control valve 41 is controlled to be switched on to connect the
And S400, controlling a second plunger pump to spit out the sample liquid, and providing a section of auxiliary deionized water column for removing bubbles for the sample liquid by a pipeline of the precise sample adding system in the process of spitting out the sample liquid so that the sample liquid is precisely and completely spit out.
According to the precise sample adding system, degassed constant-temperature deionized water is adopted to clean the inner wall of the probe and the inner wall of the reaction vessel, so that the reaction environment is highly clean, meanwhile, a unique pipeline connection configuration mode is adopted, the precision of the sample dropping amount is greatly improved, and the accuracy of the final detection result is improved by matching the high-precision sample dropping amount with the highly clean reaction environment. The cleaning effect is greatly enhanced while the sample injection quantity precision is improved, the accuracy of the final analysis result of the optical system can be obviously improved, and the defects that the measured value of the in-vitro detection equipment is unstable and inaccurate are overcome.
In addition, the embodiment of the invention also provides in-vitro diagnosis equipment which comprises the precise sample adding system. The in-vitro diagnostic equipment can be a biochemical analyzer or other commonly used in-vitro diagnostic equipment.
In summary, the precise sample adding system, the in-vitro diagnostic device and the precise sample adding method provided by the invention have a unique pipeline connection configuration mode, and can provide a section of auxiliary deionized water column for the sample liquid in the process of spitting the sample liquid, so that the difference caused by the dead volume and the repeated movement in the plunger pump is eliminated, the sample liquid is precisely and completely spitted, and meanwhile, the constant-temperature degassing mechanism is adopted to remove bubbles in the deionized water, so that the influence on the liquid compression in the action process of the plunger pump is reduced to the minimum, and the process precision of extruding the liquid drop sample by the plunger pump is ensured to be higher.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
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