阅读说明：本技术 一种微小型模式生物流水式毒理实验用实验杯及药液缸 (Microminiature mode biological running water type experiment cup and liquid medicine jar for toxicological experiment ) 是由 张琼 陆阳 于 2020-06-01 设计创作，主要内容包括：本发明的一种微小型模式生物流水式毒理实验用实验杯及药液缸,实验杯包括杯体、杯口和至少一组过滤缝网,所述杯体为透明不透水杯体,所述杯口作为入水孔或在杯壁上设置入水孔,所述过滤缝网位于所述杯壁下部,每组所述过滤缝网包括并排排列的多条间隙孔,所述间歇孔的宽度为0.25～0.35mm。药液缸包括外缸和多个实验杯。该实验杯容易清洗干净,杯内的药液自然排净,且由于杯体为敞口、透明的,便于用户统计大型溞的数量,实验结果的可比性提高。(The invention discloses an experimental cup and a liquid medicine cylinder for a micro-miniature mode biological running water type toxicological experiment, wherein the experimental cup comprises a cup body, a cup opening and at least one group of filtering seam nets, the cup body is a transparent and waterproof cup body, the cup opening is used as a water inlet hole or the wall of the cup is provided with the water inlet hole, the filtering seam nets are positioned at the lower part of the wall of the cup, each group of filtering seam nets comprise a plurality of gap holes which are arranged side by side, and the width of each gap hole is 0.25-0.35 mm. The liquid medicine jar includes outer jar and a plurality of laboratory cups. This experiment cup sanitization easily, the liquid medicine in the cup is drained naturally, and because the cup is uncovered, transparent, the user of being convenient for makes statistics of the quantity of daphnia magna, the comparability of experimental result improves.)

1. The micro-miniature biological running water type experimental cup for toxicology experiments is characterized by comprising a cup body, a cup opening and at least one group of filtering seam nets, wherein the cup body is transparent and impermeable, the cup opening is used as a water inlet hole or is provided with a water inlet hole on the cup wall, the filtering seam nets are positioned on the lower portion of the cup wall, each group of filtering seam nets comprise a plurality of gap holes which are arranged side by side, and the width of each gap hole is 0.25-0.35 mm.

2. The micro-miniature model biological running water type toxicological experiment cup according to claim 1, wherein the plurality of sets of the filter nets are uniformly distributed on the lower part of the cup wall.

3. The micro-miniature model biological flowing water type toxicological experiment cup according to claim 1, wherein the filtering slit net is a set, and is disposed opposite to the water inlet hole disposed on the cup wall, and the plurality of gap holes are symmetrically distributed about the center of the water inlet hole.

4. The micro-miniature biological flow-through toxicological test cuvette according to claim 1, wherein the clearance hole is in the shape of a vertical bar with a width of 0.3 mm.

5. The micro-miniature biological flow-type toxicological experiment cup according to claim 1, wherein the mouth of the cup is of a trumpet shape, and the caliber of the cup gradually increases from bottom to top.

6. The micro-miniature model experiment cup for biological flowing water type toxicological experiment according to claim 1, wherein the distance between the bottom of the clearance hole and the cup bottom is 5-8 mm.

7. The micro-miniature model biological flow-through toxicological test cuvette according to claim 1, wherein the length of the clearance hole is in the range of 20-35 mm.

8. A micro-miniature biological flow type liquid medicine tank for toxicological experiments, which is characterized by comprising an outer tank and a plurality of experiment cups according to any one of claims 1 to 7;

the outer cylinder comprises a cylinder body, a cover plate, a liquid inlet distributor, a plurality of flow guide grooves and an overflow pipe;

the experiment cups are uniformly distributed on the outer edge of the interior of the cylinder body, the liquid inlet distributor is arranged in the center of the cover plate and is provided with a liquid inlet, a plurality of diversion trenches with the same length and corresponding to the number of the experiment cups are arranged on the cover plate, one side of each diversion trench is communicated with the liquid inlet of the liquid inlet distributor, and the other side of each diversion trench is communicated with the experiment cups; the overflow pipe is arranged in the center of the bottom of the cylinder body.

9. The liquid medicine cylinder for micro-miniature mode biological flowing water type toxicological experiments according to claim 8, wherein the cover plate has a plurality of openings corresponding to the number of the experiment cups, and the upper part of each experiment cup passes through each opening and is exposed out of the cover plate; the tail end of the other side of the flow guide groove is provided with a clamping groove, and a hoop corresponding to the water inlet hole of each experiment cup is arranged on each clamping groove.

10. The micro-miniature biological fluid type liquid medicine cylinder for toxicological experiments according to claim 8, wherein the number of the experiment cups is 4-16.

Technical Field

The invention relates to the field of micro-miniature aquatic model organism breeding, in particular to a micro-miniature model organism running water type experiment cup and a liquid medicine tank for toxicological experiments.

Background

The daphnia magna is a cladocera invertebrate, yellow or light red, and slightly transparent, and the mother daphnia magna produces daphnia magna once in three days. The body length is tiny, the female body length is 2.2-4.8 mm, the male body length is 1.75-2.50 mm, and the method belongs to a tiny aquatic organism. The method has the characteristics of short life cycle, fast propagation, economy, easy laboratory culture, very sensitive change of various chemical substances in water environment, easy observation of change and the like, and is widely used for environmental toxicology and drug toxicity tests in the world as a model organism. The method comprises the steps of putting the daphnia magna of the daphnia magna into a solution containing detected substances for cultivation for a period of time, then observing the growth state and the propagation and development of the daphnia magna, finally calculating to obtain the survival rate and the death rate data of the daphnia magna, and further obtaining the pollution degree of water quality or the aquatic toxicity of a tested substance.

In order to ensure the scientificity and effectiveness of the experiment and the comparability of the experimental results, most of the methods require that the tested substance can be kept relatively stable in a test system in the whole test period, so that the influence of other factors on the growth, reproduction and development of daphnia magna is eliminated as much as possible, and the influence of the toxicity of the liquid medicines with different concentrations on daphnia magna is evaluated, for example, a toxicological experiment needs to compare the toxicity difference among the liquid medicines with different concentrations through the reproduction statistical comparison value of the total amount of daphnia magna in a cultivation cylinder with the liquid medicines with different concentrations in the 21-day test period.

In a toxicological test using micro-model organisms, a plurality of test cups in a liquid medicine tank are used for culturing micro-aquatic organisms, and the same amount of liquid medicine needs to be put into each test cup and the liquid medicine in each test cup needs to be updated in an equal amount. The existing experimental cup is a stainless steel mesh cup surrounded by a 60-mesh stainless steel mesh. The problems that exist are: the method is characterized in that green algae is required to be continuously fed when the daphnia magna is cultured, the residual green algae are easy to stick on the holes after a long time, on one hand, the flowing liquid medicine is not facilitated to flow due to the blockage of fine meshes, on the other hand, the cleaning is not easy, and the comparability of experimental results is influenced due to the difference of water permeability of different experimental cups; the stainless steel cup is dark in color and poor in light transmission, and a user is very difficult to count the quantity of daphnia magna by naked eyes, so that the accuracy of results is also influenced.

Disclosure of Invention

The invention provides a micro-miniature biological running water type toxicology experiment cup and a liquid medicine cylinder, aiming at solving the problems that a stainless steel cup is easy to block by green algae, difficult to clean, poor in light transmittance and influence the comparability and accuracy of results.

The technical scheme of the invention is as follows:

the utility model provides a biological running water formula of microminiature experiment cup for toxicology, includes cup, rim of a cup and at least a set of filtration seam net, the cup is transparent impervious, the rim of a cup is as the inlet hole or sets up the inlet hole on the wall of a cup, the filtration seam net is located the wall of a cup lower part, every group the filtration seam net is including many interstitial hole of arranging side by side, the width in intermittent type hole is 0.25 ~ 0.35 mm.

The filter seam nets are in multiple groups and are uniformly distributed on the lower part of the cup wall.

The filter seam nets are a group and are arranged opposite to the water inlet holes formed in the wall of the cup, and the plurality of clearance holes are symmetrically distributed about the centers of the water inlet holes.

The clearance hole is in a vertical strip shape, and the width of the clearance hole is 0.3 mm.

The cup mouth is in a horn shape, and the caliber of the cup mouth is gradually increased from bottom to top.

The distance between the bottom of the clearance hole and the cup bottom is 5-8 mm.

The length of the clearance hole ranges from 20 mm to 35 mm.

A micro-miniature biological flow-type liquid medicine tank for toxicological experiments comprises an outer tank and a plurality of experiment cups; the outer cylinder comprises a cylinder body, a cover plate, a liquid inlet distributor, a plurality of flow guide grooves and an overflow pipe; the experiment cups are uniformly distributed on the outer edge of the interior of the cylinder body, the liquid inlet distributor is arranged in the center of the cover plate and is provided with a liquid inlet, a plurality of diversion trenches with the same length and corresponding to the number of the experiment cups are arranged on the cover plate, one side of each diversion trench is communicated with the liquid inlet of the liquid inlet distributor, and the other side of each diversion trench is communicated with the experiment cups; the overflow pipe is arranged in the center of the bottom of the cylinder body.

The cover plate is provided with a plurality of openings corresponding to the number of the experimental cups, and the upper part of each experimental cup correspondingly penetrates through each opening and is exposed out of the cover plate; the tail end of the other side of the flow guide groove is provided with a clamping groove, and a hoop corresponding to the water inlet hole of each experiment cup is arranged on each clamping groove.

The number of the experimental cups is 4-16.

The invention has the beneficial technical effects that:

the invention discloses a micro-miniature biological flow type experiment cup for toxicological experiments, which is different from the prior stainless steel mesh cup in an open and opaque structure. Therefore, the updated liquid medicine and/or water is introduced into the experiment cup through the cup opening or the water inlet hole on the cup wall, and meanwhile, the cup opening is convenient for observing the state of the micro-mode organisms (such as daphnia) in the cup in real time; a plurality of clearance holes with the width of 0.25-0.35 mm which are arranged side by side at the lower part of the cup wall form a filtering seam net for water passing, and simultaneously, micro-miniature mode organisms are guaranteed to be trapped in the experiment cup, so that a flowing water type culture mode of the micro-miniature organisms is realized. And if foreign matters are stuck on the clearance holes arranged on the wall of the glass or resin cup, the glass or resin cup is easily cleaned by a small brush, and compared with the stainless steel mesh cup which cannot be cleaned, the stainless steel mesh cup is poor in comparability of experimental results, and the experimental result comparability of the invention is improved. On the other hand, after the experimental cup is taken out due to the arrangement of the clearance holes, the liquid medicine in the cup is naturally discharged, and the cup body is open and transparent, so that a user can conveniently count the quantity of daphnia magna.

The filter seam net can be distributed on the lower part of the cup wall in a plurality of groups, namely holes are formed in the cup body in multiple directions, so that the liquid medicine is more favorably updated and exchanged; or the filtering seam nets are in a group and are arranged opposite to the water inlet holes in the cup wall, so that the processing cost is greatly reduced on the premise of updating the liquid medicine. The distance between the bottom of the clearance hole and the cup bottom is 5-8mm, the length range of the clearance hole is 20-35mm, water flowing is facilitated to be formed, the flowing mode is met, and meanwhile, after the experiment cup is taken out, liquid medicine in the cup is naturally discharged.

When the filter screen is used for culturing daphnia magna, the clearance holes forming the filter seam net are vertical strips, the width is 0.3mm, the algae liquid cannot run off along with water flow, and the feeding effect is ensured; after the daphnia is bred and propagated, the daphnia magna cannot escape along with water flow, and the propagation effect is ensured.

The cup mouth is horn type, is convenient for the cup body to get and put.

The invention relates to a micro-miniature biological flowing water type liquid medicine cylinder for toxicological experiments. The two can be combined in a way that a traditional Chinese medicine liquid cylinder with the patent application number of 201720919422.8 is matched with an experimental cup. The experimental cup can also be combined in an improved mode, namely, the water inlet hole at the upper part of the cup wall of the experimental cup is higher than the cover plate, the cup mouth of the experimental cup is exposed outside the cover plate of the outer cylinder, and the experimental cup is hung on the clamping groove at the tail end of the diversion trench through the water inlet hole on the cup wall, so that on one hand, the state of microminiature organisms in the cup can be conveniently observed from the cup mouth; on the other hand, not only draws water into the cup through the inlet hole, has the constant head tank function simultaneously, ensures to be in more stable state in the experiment cup. Inject the liquid medicine into feed liquor distributor, feed liquor distributor utilizes the same footpath distribution hole, utilize gravity just can distribute liquid medicine equivalent to multichannel guiding gutter on the cylinder cap, rethread guiding gutter and income water hole carry the liquid medicine to the inboard of laboratory cup, the liquid medicine falls into the outside jar edge of filter seam net inflow that the clearance hole in the laboratory cup outside formed after the laboratory cup, reverse inflow again is located the overflow pipe at outer jar middle part, when reacing overflow pipe upper portion, get into the outer jar of overflow pipe discharge, from forming directional flow, in order to impel the slow even flow of liquid medicine, do benefit to the liquid medicine and continuously circulate and update.

Drawings

FIG. 1 is a front view of an embodiment of the laboratory cup of the present invention;

FIG. 2 is a side view of an embodiment of the laboratory cup of the present invention;

FIG. 3 is a top view of FIG. 2;

FIG. 4 is a perspective view of a liquid drug container with ten test cups;

FIG. 5 is a schematic view showing the flow direction of the liquid chemical in the liquid chemical tank.

The reference numbers: 1-an experimental cup; 1-1-water inlet hole; 1-2-mesopores; 1-3-cup mouth; 2-outer cylinder; 2-1-cover plate; 2-2-diversion trench; 2-3-liquid inlet; 2-4-overflow pipe; 2-5-a liquid inlet distributor; 2-6-card slot; 2-7-opening.

Detailed Description

The following will describe a micro-miniature biological flow-type toxicological test cup and a liquid medicine tank according to the present invention in detail with reference to the accompanying drawings 1-5 and the specific embodiments.