阅读说明：本技术 一种预防育肥公羊尿结石的微生物有益活菌剂及其制备方法 (Microbial beneficial live bacterium agent for preventing urinary calculus of fattening ram and preparation method thereof ) 是由 张元生 张海员 于 2020-08-13 设计创作，主要内容包括：本发明公开了一种预防育肥公羊尿结石的微生物有益活菌剂及其制备方法。其中,所述微生物有益活菌剂包括以下重量份的原料：乳酸菌1～3份、酵母菌2～4份、光和细菌1～3份、解磷菌2～4份、培养基8～12份和井水60～100份。本微生物有益活菌剂只需要在饲料中添加即可,利用复合益生菌使饲料中无机磷被羊体充分吸收,减少羊体内磷酸盐产生量,并达到预防尿结石的目的。极大减少了养殖户的经济损失。(The invention discloses a microbial beneficial live bacterium agent for preventing urinary calculus of fattening rams and a preparation method thereof. The microorganism beneficial live bacteria agent comprises the following raw materials in parts by weight: 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 2-4 parts of phosphate solubilizing bacteria, 8-12 parts of culture medium and 60-100 parts of well water. The beneficial microbial live bacteria agent can be added into feed only, inorganic phosphorus in the feed is fully absorbed by sheep bodies by using the composite probiotics, the generation amount of phosphate in the sheep bodies is reduced, and the aim of preventing urinary calculus is fulfilled. Greatly reducing the economic loss of farmers.)

1. The microbial beneficial live agent for preventing the urinary calculus of the fattening ram is characterized by comprising the following raw materials in parts by weight:

1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 2-4 parts of phosphate solubilizing bacteria, 8-12 parts of culture medium and 60-100 parts of well water.

2. The microbial beneficial live agent for preventing and treating the urinary calculus of the fattening ram as claimed in claim 1, wherein the microbial beneficial live agent comprises the following raw materials in parts by weight:

2 parts of lactic acid bacteria, 3 parts of saccharomycetes, 2 parts of light and bacteria, 3 parts of phosphate solubilizing bacteria, 10 parts of culture medium and 80 parts of well water.

3. The live microbial benefit agent for preventing urinary calculi of fattening rams according to claim 1 or 2, characterized in that the live microbial benefit agent is prepared by a preparation method comprising the following steps:

(1) putting well water into a container, heating to 45 ℃, putting a culture medium, lactic acid bacteria, saccharomycetes, light bacteria and phosphate-solubilizing bacteria into the container in sequence, and fully and uniformly stirring to obtain a compound bacteria liquid;

(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;

(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of seed liquors in equal parts, and expanding and propagating the seed liquors to 10 times to obtain the beneficial microorganism bacterium agent.

4. The live microbial benefit agent for preventing urinary calculi in fattening rams according to claim 3, further comprising:

(4) the microorganism beneficial live bacteria agent is added into the feed according to the proportion of 0.4 percent to prepare the microorganism beneficial live bacteria agent feed for preventing the urinary calculus of the fattening rams.

5. A preparation method of a microorganism beneficial live bacteria agent for preventing urinary calculus of fattening rams is characterized by comprising the following steps:

(1) putting well water into a container, heating to 45 ℃, putting a culture medium, lactic acid bacteria, saccharomycetes, light bacteria and phosphate-solubilizing bacteria into the container in sequence, and fully and uniformly stirring to obtain a compound bacteria liquid;

wherein, the raw materials are proportioned according to the following weight portions: 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 2-4 parts of phosphate solubilizing bacteria, 8-12 parts of culture medium and 60-100 parts of well water;

(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;

(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of seed liquors in equal parts, and expanding and propagating the seed liquors to 10 times to obtain the beneficial microorganism bacterium agent.

6. The preparation method of the microbial beneficial live agent for preventing the urinary calculus of the fattening ram as claimed in claim 5, wherein the preparation method comprises the following steps:

2 parts of lactic acid bacteria, 3 parts of saccharomycetes, 2 parts of light and bacteria, 3 parts of phosphate solubilizing bacteria, 10 parts of culture medium and 80 parts of well water.

7. The preparation method of the microbial beneficial agent for preventing the urinary calculus of the fattening ram as claimed in claim 5, wherein the preparation method further comprises the following steps:

(4) the microorganism beneficial live bacteria agent is added into the feed according to the proportion of 0.4 percent to prepare the microorganism beneficial live bacteria agent feed for preventing the urinary calculus of the fattening rams.

Technical Field

The invention belongs to the technical field of animal nutrition and feed science, and particularly relates to a microbial beneficial live bacterium agent for preventing and fattening ram urinary calculus and a preparation method thereof.

Background

With the improvement of the living standard of human beings, the demand of meat is large. Recently, due to the fact that African swine fever has been abused, the market demand for mutton is larger and larger, the demand for mutton is short in recent years as the price of mutton is high, and farmers start to breed sheep in a large scale with great support of governments, but breeding problems are endless.

Among them, sheep urinary calculus is one of the troublesome problems which plague sheep breeding. In the ram fattening process, the ram fattening feed is high in phosphorus content, calcium deficiency and imbalance in proportion of calcium to phosphorus due to the fact that a large amount of high-protein, high-energy and high-phosphorus feed is fed, so that the mutton sheep generate excessive phosphate crystals in the urinary tract during the fattening period to form urinary calculus, block the urinary tract and cause difficulty in urination. The attention of breeders is only given when urethral calculus completely or incompletely blocks the urethra, so that sheep urinating pain and frequent micturition are caused. And when urinary calculus occurs, the ram usually has no body temperature change, and if urinary calculus causes bladder rupture, the ram is depressed and died within a few days. The incidence of urinary calculus of the bred male goats is high.

Therefore, the invention aims to provide the method for preventing the urinary calculus of the fattening ram, which has low cost, does not increase the burden on farmers and can also improve the breeding income.

Disclosure of Invention

The invention aims to solve the technical problems and provides a microbial beneficial live bacterium agent for preventing and fattening ram urinary calculus and a preparation method thereof.

In order to solve the problems, the invention is realized according to the following technical scheme:

the invention relates to a microbial beneficial live bacterium agent for preventing urinary calculus of fattening rams, which comprises the following raw materials in parts by weight:

1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 2-4 parts of phosphate solubilizing bacteria, 8-12 parts of culture medium and 60-100 parts of well water.

Preferably, the microorganism beneficial live agent comprises the following raw materials in parts by weight:

2 parts of lactic acid bacteria, 3 parts of saccharomycetes, 2 parts of light and bacteria, 3 parts of phosphate solubilizing bacteria, 10 parts of culture medium and 80 parts of well water.

Preferably, the microbial beneficial live agent is prepared by a preparation method comprising the following steps:

(1) putting well water into a container, heating to 45 ℃, putting a culture medium, lactic acid bacteria, saccharomycetes, light bacteria and phosphate-solubilizing bacteria into the container in sequence, and fully and uniformly stirring to obtain a compound bacteria liquid;

(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;

(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of seed liquors in equal parts, and expanding and propagating the seed liquors to 10 times to obtain the beneficial microorganism bacterium agent.

Preferably, the preparation method further comprises:

(4) the microorganism beneficial live bacteria agent is added into the feed according to the proportion of 0.4 percent to prepare the microorganism beneficial live bacteria agent feed for preventing the urinary calculus of the fattening rams.

The invention also provides a preparation method of the microbial beneficial live bacteria agent for preventing the urinary calculus of the fattening ram, which comprises the following steps:

(1) putting well water into a container, heating to 45 ℃, putting a culture medium, lactic acid bacteria, saccharomycetes, light bacteria and phosphate-solubilizing bacteria into the container in sequence, and fully and uniformly stirring to obtain a compound bacteria liquid;

wherein, the raw materials are proportioned according to the following weight portions: 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 2-4 parts of phosphate solubilizing bacteria, 8-12 parts of culture medium and 60-100 parts of well water;

(2) standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;

(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of seed liquors in equal parts, and expanding and propagating the seed liquors to 10 times to obtain the beneficial microorganism bacterium agent.

Preferably, the raw materials are proportioned according to the following parts by weight: 2 parts of lactic acid bacteria, 3 parts of saccharomycetes, 2 parts of light and bacteria, 3 parts of phosphate solubilizing bacteria, 10 parts of culture medium and 80 parts of well water.

Preferably, the preparation method further comprises:

(4) the microorganism beneficial live bacteria agent is added into the feed according to the proportion of 0.4 percent to prepare the microorganism beneficial live bacteria agent feed for preventing the urinary calculus of the fattening rams.

Compared with the prior art, the invention has the beneficial effects that:

the raw materials required by the microbial beneficial live bacterium agent for preventing and fattening the ram urinary calculus comprise lactic acid bacteria, saccharomycetes, light bacteria, phosphate solubilizing bacteria, culture medium, well water and the like, the sources are wide, the price is low, the preparation process is simple, and the cost of farmers is greatly reduced. Moreover, the beneficial microorganism live bacteria agent only needs to be added into the feed, and the inorganic phosphorus in the feed is fully absorbed and utilized by utilizing the composite probiotics, so that the generation amount of phosphate in sheep bodies is effectively reduced, and the aim of preventing urinary calculus is fulfilled. Greatly reducing the economic loss of farmers.

Detailed Description

The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.

The application provides a microbial beneficial live bacterium agent for preventing urinary calculus of fattening rams, which is added into feed. By using the composite probiotics, the inorganic phosphorus in the feed is fully absorbed and utilized, the generation amount of phosphate in sheep bodies can be effectively reduced, and the aim of preventing urinary calculus is fulfilled. The composite probiotics only adopt lactic acid bacteria, yeast, light and bacteria and phosphate-solubilizing bacteria. The invention properly adopts various probiotics, starts from a plurality of angles, reduces the risk of possibly causing the urinary calculus, and can effectively prevent the mutton sheep urinary calculus under the combined action of all factors.

In a first aspect, the invention provides a microbial beneficial live bacterium agent for preventing urinary calculus of a fattening ram, which comprises the following raw materials in parts by weight: 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 2-4 parts of phosphate solubilizing bacteria, 8-12 parts of culture medium and 60-100 parts of well water.

The invention provides a urinary calculus prevention device for multiple angles, which comprises the following technical means:

1. decomposing inorganic phosphorus in the feed: the adopted composite probiotics can decompose and utilize inorganic phosphorus in the feed, and fully decompose the inorganic phosphorus in the sheep body.

2. Increasing the water intake of sheep: the saccharomycetes can improve the palatability of the feed, improve the feeding and fattening of the sheep, further promote the sheep to drink more water, increase the water drinking amount and prevent urethral calculus of the sheep.

3. Adjusting the pH value of urine: herbivore urine is typically high in pH and alkaline. Do not facilitate the dissolution of struvite, calcium phosphate and calcium carbonate stones and the use of an acidifying agent is beneficial in preventing the occurrence of stones. And the feed is softened and acidified by lactic acid bacteria, so that the pH value in sheep intestinal tracts is increased, and urine is acidified.

4. And (3) vitamin supplement: the light and bacteria are also called photosynthetic bacteria, and the light and bacteria and lactic acid bacteria can generate vitamins and prevent urinary calculus.

In the invention, drinking water wells are generally arranged in farmers and are natural water, and the well water is mainly used as a solvent and used for mixing various raw materials. The well water contains less chemical agents such as disinfectants and the like, and is more suitable for feeding.

In a second aspect, the invention provides a preparation method of a microbial beneficial live bacterium agent for preventing urinary calculus of fattening rams and a preparation method of a prepared feed, wherein the preparation method comprises the following steps:

(1) putting well water into a container, heating to 45 ℃, putting a culture medium, lactic acid bacteria, saccharomycetes, light bacteria and phosphate-solubilizing bacteria into the container in sequence, and fully and uniformly stirring to obtain a compound bacteria liquid;

wherein, the raw materials are proportioned according to the following weight portions: 1-3 parts of lactic acid bacteria, 2-4 parts of saccharomycetes, 1-3 parts of light and bacteria, 2-4 parts of phosphate solubilizing bacteria, 8-12 parts of culture medium and 60-100 parts of well water;

the medium is a medium that can be selected by those skilled in the art and will not be described herein.

(2) Standing and fermenting the mixed solution for 24 hours in a constant temperature environment, wherein the temperature of the constant temperature environment is 30 ℃, and the fermented composite bacteria liquid is obtained to obtain composite bacteria mother liquor;

(3) adding brown sugar into the composite bacterium mother liquor, mixing, dividing the composite bacterium mother liquor into a plurality of seed liquors in equal parts, and expanding and propagating the seed liquors to 10 times to obtain the beneficial microorganism bacterium agent;

(4) the microorganism beneficial live bacteria agent is added into the feed according to the proportion of 0.4 percent to prepare the microorganism beneficial live bacteria agent feed for preventing the urinary calculus of the fattening rams.

Specifically, the microorganism beneficial live bacteria agent is added according to 0.4 percent of the weight of the feed.

In the above preparation method, the feed may be a general feed or a concentrated feed. The composite probiotics in a certain proportion are added into the feed, and the inorganic phosphorus in the feed is fully absorbed and utilized by the composite probiotics, so that the generation amount of phosphate in sheep bodies is effectively reduced, and the aim of preventing urinary calculus is fulfilled. And the adopted composite probiotics have low cost, can save the culture cost and prevent urinary calculus.

Preferably, the feed comprises grass hay of the family Gramineae, and long-stem forage grass can be adopted, and the long-stem forage grass can increase the secretion of saliva, so that more phosphorus is discharged out of the body along with excrement. Is beneficial to preventing and fattening the male goat urinary calculus.