Device for freezing adipose tissue
阅读说明:本技术 用于冷冻脂肪组织的装置 (Device for freezing adipose tissue ) 是由 L·帕梅吉亚尼 S·劳雷蒂 于 2019-02-01 设计创作,主要内容包括:一种用于冷冻脂肪组织的装置(1),其包括第一构件(2)和第二构件(3),它们相互可联接以便获得至少一个封闭构造;第一构件包括至少一个手动抓握部分(4)和供脂肪组织粘附至其的至少一个支承部分(5);第二构件包括供第一构件的至少支承部分插设在其中的至少一个壳体(6)。第二构件包括至少一个安全阀(20),该安全阀(20)包括至少一个可分离部分(21),该可分离部分(21)适于在内部和外部之间的压力差超过预定值的情况下从所述第二构件的主体部分地或完全地脱离。一旦可分离部分(21)已经脱离,则第二构件包括可分离部分(21)的保持装置(24)。可分离部分的脱离在第二构件中形成开口(26),并且限定用于排放被包含在壳体(6)内的气体的间隙(25)。(Device (1) for freezing adipose tissue, comprising a first member (2) and a second member (3) mutually couplable so as to obtain at least one closed configuration; the first member comprises at least one manual gripping portion (4) and at least one supporting portion (5) to which the adipose tissue adheres; the second member comprises at least one housing (6) in which at least the support portion of the first member is inserted. The second component comprises at least one safety valve (20), the safety valve (20) comprising at least one detachable portion (21), the detachable portion (21) being adapted to be partially or completely detached from the body of said second component in case the pressure difference between the inside and the outside exceeds a predetermined value. Once the detachable portion (21) has been detached, the second member comprises retaining means (24) of the detachable portion (21). Disengagement of the separable portion forms an opening (26) in the second member and defines a gap (25) for discharging gas contained within the housing (6).)
1. A device (1) for freezing adipose tissue, said device (1) comprising a first member (2) and a second member (3), said first member (2) and said second member (3) being mutually couplable so as to obtain at least one closed configuration of cryopreservation of adipose tissue, said first member (2) comprising at least one manual gripping portion (4) and at least one supporting portion (5), wherein said adipose tissue is adhered to said supporting portion (5), said second member (3) comprising at least one shell (6), at least said supporting portion (5) of said first member (2) being insertable in said shell (6), said second member (3) comprising at least one safety valve (20), said at least one safety valve (20) being adapted to be activated in the event of a difference between an external pressure and an internal pressure of said shell (6) exceeding a predetermined value, wherein the safety valve (20) comprises at least one detachable portion (21) of the second member (3), said at least one detachable portion (21) being adapted to be partially or completely detached from the body of said second member (3) in case the pressure difference between the inside and the outside exceeds a predetermined value, and wherein the detachable portion (21) is delimited by a frangible region (22), characterized in that said second member (3) comprises retaining means (24) of said detachable portion (21) once said detachable portion (21) is detached, and the disengagement of the detachable portion (21) determines the formation of an opening (26) in the second member (3), a gap (25) for discharging the gas contained in the housing (6) is defined between the opening (26) and the detachable portion (21) held by the holding means (24).
2. Device according to claim 1, characterized in that said frangible zone (22) is defined by a notch (23) made in said second member (3) so as to obtain a weakened zone which breaks when a certain mechanical stress is reached due to the difference between the internal and external pressure.
3. Device according to claim 2, characterized in that said notch (23) is circular or substantially circular and is made in the base (3a) of said second member (3).
4. A device according to claim 2 or 3, characterized in that the recess (23) has a triangular cross-section.
5. Device according to any one of the preceding claims, characterized in that said retaining means (24) are adapted to form an undercut between said detachable portion (21) and said hole (26), so as to prevent the detachment between the two parts.
6. The device according to claim 5, characterized in that said retaining means (24) comprise at least one appendage (27) of said detachable portion (21), said at least one appendage (27) being shaped so as to abut against an inner surface (6a) of said casing (6).
7. Device according to claim 6, characterized in that said appendage (27) has a substantially divergent shape and in that the divergence proceeds from a connection zone (27a) with said detachable portion (21) towards a free end (27 b).
8. Device according to claim 6 or 7, characterized in that said retaining means (24) comprise a plurality of said appendages (27), said plurality of appendages (27) being angularly spaced with respect to each other with respect to the centre of said detachable portion (21).
9. The device according to any one of the preceding claims, comprising: -a constraint device (15) of the snap coupling type of the first member (2) to the second member (3).
10. Device according to any one of the preceding claims, characterized in that said supporting portion (5) comprises at least one substantially flat elongated element (13), said elongated element (13) having a given concave shape.
11. Device according to the preceding claim, characterized in that said elongated element (13) comprises a plurality of holes (14) adapted to improve its thermal conductivity.
12. Liquid nitrogen container (31) comprising at least one support (29, 30) for a plurality of devices (1) for freezing adipose tissue according to one of claims to 11.
13. Container according to claim 12, characterized in that it comprises a first support (29) and a second support (30) provided by respective first and second shaped apertures (32, 33), into which first and second supports (29, 30) respective devices (1) can be inserted.
14. Container according to claim 13, characterized in that the second member (3) of the device (1) comprises at least one coupling portion (28) coupled to the first support (29), the coupling portion (28) comprising at least one peripheral groove (28a) in which an edge of a respective first shaped aperture (32) can engage.
15. The container according to claim 14, wherein the grip portion (4) of the first member (2) and the second member (3) of the device (1) have a substantially prismatic shape, with a triangular section with rounded vertices, each first shaped aperture (32) having the same shape as the section of the second member (3), each second shaped aperture (33) having a three-lobed shape to allow each device (1) to rotate about its own longitudinal axis to engage with the edge of the corresponding first shaped aperture (32) in the respective groove (28 a).
Technical Field
The present invention relates to a device for freezing adipose tissue.
More specifically, the invention relates to a device for freezing adipose tissue 5, which is suitable for being immersed in liquid nitrogen.
Background
Human adipose tissue is a source of stem cells, which is an ideal choice for autologous cell therapy. The preferred conditions for tissue engineering and regenerative medicine applications are "fresh" cells and cell products that are immediately available from a clinical perspective.
Adipose tissue may be obtained from a site that accumulates within the body and immediately subsequently transplanted back into the site to be treated in the same patient.
Autologous adipose tissue was removed by liposuction. To avoid repeating liposuction procedures multiple times, adipose tissue may be cryopreserved in a sterile manner for subsequent re-implantation.
The re-transplantation of frozen/thawed adipose tissue has not been widely used clinically, but certain freezing techniques enable the obtaining of thawed adipose tissue with the same function as fresh tissue.
Cell and tissue freezing protocols aim to prevent the formation of intracytoplasmic water crystals, which would otherwise damage the cytoskeleton.
This can be achieved by vitrification, which refers to the transition to a glassy state when a high viscosity liquid is cooled below the glass transition temperature.
Any material can be vitrified, which is directly dependent on its viscosity and cooling rate, and vice versa on the volume of the sample.
In the case of cell vitrification, viscosity is increased by dehydration and high cooling rates are ensured by direct immersion in liquid nitrogen; for this purpose, specific devices for the vitrification of individual cells have been developed.
Vitrification techniques have been widely used in the field where frozen survival must be maximized, particularly in human embryology for oocytes and embryos.
In particular, human germ cells and tissues are frozen by vitrification induced by direct immersion in liquid nitrogen; for this purpose, the protocols and devices are used in a way that reduces the risk of contamination of the biological sample and are also manufactured in a way that reduces the risk of frostbite to which germ cells and tissues are particularly exposed due to their abundance in intracellular water.
An example of a device for vitrifying germ cells or tissues by immersion in liquid nitrogen is described in document EP 2765183B 1.
The device generally comprises an elongate-shaped container, also called stopper, open on one side, and a support for cells or tissue insertable into the opening of such a container.
A support for cells or tissue comprising in sequence: elongated portion-usually made of a material with good thermal conductivity (such as metal) -to which cells or tissue adhere themselves; and a grip portion (associated with the elongate portion) which remains on the exterior of the container when the two parts are joined.
Protocols for freezing adipose tissue studied to date are those envisaged for mechanical cold storage in liquid nitrogen, rapid freezing or slow controlled freezing.
As the technology described above, which is currently used for germ cells or tissues, can provide satisfactory results and can guarantee significant advantages from an infinite number of points of view (for example, from the cost of the device, the practicality of use, the operating times and others), the operators of the industry feel the need to be able to invoke it in different types of applications, and mainly-but not exclusively-in cryopreservation of adipose tissue in order to exploit the characteristics and capabilities of the mesenchymal cells located between the adipocytes.
Object of the Invention
Accordingly, the technical scope of the present invention is to improve the prior art in the field of freezing and cryopreservation of adipose tissue.
Within this technical scope, the object of the present invention is to develop a device that also allows the use of the technique of direct immersion in liquid nitrogen for the cryopreservation of adipose tissue.
Another object of the present invention is to provide a device for freezing adipose tissue that is reliable and safe to use, in particular (and not exclusively) from the point of view of eliminating the risk of contamination or loss of removed tissue and from the point of view of operator safety.
It is another object of the present invention to provide a device that is improved and specifically developed for optimal freezing of adipose tissue.
It is a further object of the present invention to provide a device for freezing adipose tissue that is particularly practical and comfortable to use.
It is another object of the present invention to provide a device for freezing adipose tissue that is simple and inexpensive to construct.
This scope and these objects are all achieved by a device for freezing adipose tissue according to the appended
The device comprises a first member and a second member which are mutually couplable in order to obtain at least one closed configuration of cryopreservation of adipose tissue.
The first member includes at least one manual gripping portion and at least one support portion to which adipose tissue is adhered.
The second member includes at least one housing in which at least the support portion of the first member is insertable.
The second component comprises at least one safety valve adapted to activate when the difference between the external pressure and the internal pressure of the housing of the second component exceeds a predetermined value; such a safety valve comprises at least one detachable portion of the second member adapted to be partially or completely detached from the body of the second member if the pressure difference between the inside and the outside exceeds a predetermined value; the separable portion is defined by a frangible region.
According to one aspect of the invention, once the detachable portion has been detached, the second member comprises retaining means of the detachable portion; disengagement of the separable portion may determine that an opening is formed in the second member, thus defining a gap between the opening and the separable portion held by the holding means for discharging the gas contained in the housing.
In this way, if for some reason the pressure inside the casing increases (for example if any liquid nitrogen droplets that have accidentally penetrated inside the casing expand in an uncontrolled manner), the presence of the safety valve allows, on the one hand, to discharge the gas contained in the casing to the outside through the above-mentioned gap, thus protecting the operator, and, on the other hand, to allow the same safety valve to retain the fatty tissue still inside the casing, preventing its dispersion. The dependent claims relate to preferred and advantageous embodiments of the invention.
Drawings
These and other advantages will be better understood by those skilled in the art from the following description, provided by way of non-limiting example, with the aid of the following drawings, in which:
FIG. 1 is an exploded isometric view of a device according to the present invention;
FIG. 2 is an exploded isometric view of the device at various angles;
figures 3A to 3F are also isometric views of the device at different angles;
FIG. 4 is a side view of the device;
FIG. 4A is a front view of the device;
FIG. 4B is a rear view of the device;
FIGS. 4A to 4I are sectional views of the device according to plane C-C, D-D, E-E, F-F, G-G, H-H, I-I, respectively, of FIG. 4;
FIG. 5 is a side view and a partial cross-sectional view of the device;
FIG. 6 is an isometric view of the device in a stage of coupling of the first and second members;
FIG. 7 is an isometric view of the device with the first member and the second member coupled;
figure 8 is a detailed isometric view of the mouth of the housing provided in the second member;
FIG. 9 is a front view of a second member of the device;
FIG. 10 is a detailed cross-sectional side view of a second member of the device in a closed configuration;
FIG. 11 is a detailed cross-sectional side view of a second member of the device in an open configuration;
FIG. 12 is a schematic axonometric view of a plurality of devices according to the invention, immersed in a liquid nitrogen container;
FIGS. 13, 14 are plan views of details of the construction of the liquid nitrogen container;
FIG. 15 is an exploded isometric view of a device according to another embodiment of the invention;
fig. 16 is an exploded isometric view of the device of fig. 15 from a different angle.
Detailed Description
With particular reference to fig. 1, the
The
The
The
Such a closed configuration is shown, for example, in fig. 7 and 12.
The
In the embodiment shown in the figures, the grip portion 4 and the support portion 5 are constituted by two distinct elements rigidly fixed to each other.
This ultimately allows the two components to be manufactured in different materials, which materials are associated with specific use requirements, for example, having different thermal conductivities or other characteristics.
In other embodiments of the invention, the grip portion 4 and the support portion 5 may be made in a single piece.
The
When the two
The grip portion 4 comprises an
The
The grip portion 4 has a substantially prismatic shape with a triangular cross-section with rounded vertices for a more ergonomic manual grip.
The grip portion 4 thus comprises three
In its end region opposite to the
Furthermore, the grip portion 4 comprises a
At least one
The
The support part 5 can be made of a material that is a good thermal conductor.
For example, the support portion 5 may be made of a suitable metal material.
The support portion 5 comprises at least one
The
Furthermore, the
Such a concavity of the
Such concavity of the
According to another aspect of the invention, the
The presence of the holes 14 serves to improve the thermal conductivity of the
The bore 14 may have, for example, a circular cross-section or other shape.
The holes 14 may be distributed in a uniform manner or substantially uniformly over the surface of the
For example, the holes 14 may be concentrated only at the free end of the
The
For example, the
For example, as shown in fig. 5, the housing 6 provided in the
The housing 6 may have a cylindrical cross-section: this makes it possible to insert manually the
The housing 6 may also have a cross-section of different shape, for example associated with specific use requirements.
According to one aspect of the invention, the
In particular, the constraint means 15 are of the selective type, that is to say they make it possible to lock the
In the latter, therefore, the locking of the
The constraint means 15 comprise at least one
The
As shown in fig. 8, the
In this way, the
In the case where the
To unlock the
In the particular embodiment shown in the figures, the constraint means 15 comprise a plurality of
In more detail, three
Correspondingly, a plurality of
According to another aspect of the invention, and with particular reference to fig. 10, 11, the
Typically, but not exclusively, this pressure difference may be caused by the expansion of drops of liquid nitrogen accidentally deposited inside the casing 6 itself before the
Thus, in the event of such an excessive pressure difference between the inside and the outside of the
In fact, it is known that liquid nitrogen expands to about 700 times its volume when it warms, and that this does cause explosions or other uncontrollable dangerous phenomena when it compresses.
More particularly, the
The detachable portion 21 is adapted to be partially or completely detached from the main body of the
In more detail, the detachable portion 21 is provided at the
The detachable portion 21 is delimited by a frangible zone 22.
The detachable portion 21 may be, for example, substantially disc-like in shape and is therefore delimited by a circular or substantially circular frangible region 22.
As shown in fig. 10, the thickness of the fragile region 22 is smaller than the thickness of the second member 3 (particularly, the
A weak area of the
The frangible region 22 is in turn defined by a notch 23 made in the
In one embodiment of the invention, the frangible region 22 may be made of a different material than the
According to another aspect of the invention, the
The retaining means 24 prevent the detachable portion 21 from spreading into the environment in an uncontrolled manner and also allow this detachable portion 21 to remain constrained to the
Thus, disengagement of the separable portion 21 forms an opening 26 in the
The opening 26 may be constituted by, for example, a circular or other shaped opening. Thus, a gap 25 is defined between said opening 26 and the detachable portion 21 itself, the detachable portion 21 remaining constrained to the second member thanks to the retaining means 24.
In the case where the opening 26 consists of a circular hole, the gap 25 consists substantially of a circular crown, suitably sized to prevent leakage of adipose tissue from the housing 6.
This arrangement is extremely important because, as previously mentioned, it makes it possible to preserve and prevent the loss of adipose tissue and to protect the operator from any risks associated with a possible explosion of the
The triangular cross-section of the recess 23 facilitates the formation of an outwardly flared opening 26 and thus a gap 25 with a suitable surface.
The retaining means 24 is adapted to form an undercut between the detachable portion 21 and the opening 26 of the
More particularly, the retaining means 24 comprise at least one appendage 27 of the detachable portion 21.
The appendix 27 is shaped so as to abut against the inner surface 6a of the casing 6, in particular so as to abut against the inner periphery of the opening 26; an undercut is thus obtained that can prevent the detachable portion 21 from completely detaching from the
The appendage 27 has a substantially divergent shape and the divergence proceeds from the connection zone 27a with said detachable portion 21 towards the free end 27 b.
The appendage 27 can also have a certain curvature (in the direction in which the divergence proceeds) when viewed in section, so as to increase the distance from the free end 27b away from the centre of the detachable portion 21, which makes it possible to further increase the degree of undercut of the retaining means 24.
The detachable portion 21 and the accessory 27 can be made in a single piece or in separate parts connected to each other.
In the embodiment shown in the figures, the retaining means 24 more particularly comprise a plurality of appendages 27 of the aforementioned type.
Appendages 27 are angularly spaced from each other relative to the center of separable portion 21, or arranged in any other manner deemed convenient.
For example, from two to six, or even more, accessories 27 may be provided. The dimensions of the appendages 27 and their number may be suitably modified in order to obtain a gap 25 of the desired width.
As best understood by viewing fig. 11 (which shows
The
The bottom of the
Fig. 12 schematically shows a plurality of
A
In contrast, fig. 13, 14 show construction details of the
The shape and size of the
In one form of the invention, it may be provided with a
Each
The first shaped
In the embodiment shown in fig. 13, the first shaped
As shown in fig. 12, the
The edge of the first shaped
In this way, by rotating the
In contrast, the second shaped
In view of what has been described, the method of use of the
The adipose tissues to be frozen are placed on the supporting portion 5 of the
Using a
The
In the event that any liquid nitrogen droplets that have accidentally penetrated inside the casing 6 would expand in an uncontrolled manner, on the one hand the presence of the
Thus, it has been seen how the present invention achieves the intended objects.
The
Furthermore, the
Due to the size of the supporting portion 5, the device can preserve a relatively large amount of adipose tissue, a result which cannot be obtained with other known types of devices; furthermore, due to the particular shape of the support portion 5 and the presence of the holes 14 therein, the film (film) of adipose tissue is rapidly frozen. The constraint means 15 of the snap-coupling type make it possible to hermetically seal the
Last but not least, the
The invention has been described in terms of preferred embodiments, but equivalent variations are possible without departing from the scope of the appended claims.
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