Totally-enclosed reaction liquid preparation device
阅读说明:本技术 一种全密闭反应液配制装置 (Totally-enclosed reaction liquid preparation device ) 是由 刘梅 陈俊飞 万茂坤 郑典苏 唐大运 郝效禹 李国平 吴济周 于 2020-07-28 设计创作,主要内容包括:本发明涉及一种全密闭反应液配制装置,包括试剂腔壳,所述试剂腔壳上设有连接通孔、试剂腔、废液腔和液体交换器安装位,所述试剂腔底部设有试剂腔底孔,所述试剂腔上方安装上盖,所述液体交换器内设置活塞或气囊,下部连接连通器。本发明结构简单,占用空间小;工作时间短:可在2min内完成反应液配置,相比人工操作,速度可提高5-10倍,全自动配制,操作方便:将样品稀释、体积定量、试剂混合等集成在一个卡盒中,无需手动添加各种反应液成分,实现整个过程全自动化。(The invention relates to a fully-closed reaction liquid preparation device which comprises a reagent cavity shell, wherein a connecting through hole, a reagent cavity, a waste liquid cavity and a liquid exchanger installation position are arranged on the reagent cavity shell, a reagent cavity bottom hole is formed in the bottom of the reagent cavity, an upper cover is installed above the reagent cavity, a piston or an air bag is arranged in the liquid exchanger, and the lower portion of the liquid exchanger is connected with a communicating vessel. The invention has simple structure and small occupied space; the working time is short: the preparation of the reaction liquid can be completed within 2min, compared with manual operation, the speed can be improved by 5-10 times, the preparation is fully automatic, and the operation is convenient: sample dilution, volume quantification, reagent mixing and the like are integrated in one card box, various reaction liquid components do not need to be added manually, and the full automation of the whole process is realized.)
1. A totally-enclosed reaction liquid preparation device is characterized in that: the device comprises a reagent cavity shell (100), wherein a connecting through hole (109), a reagent cavity, a waste liquid cavity (110) and a liquid exchanger (400) are arranged on the reagent cavity shell (100), a reagent cavity bottom hole is formed in the bottom of the reagent cavity, an upper cover (200) is installed above the reagent cavity, a piston or an air bag is arranged in the liquid exchanger (400), and the lower part of the liquid exchanger is connected with a communicating vessel (300); the slider is driven by drive element (500), drive element (500) include mounting bracket (501), vertical installation motor (502) in mounting bracket (501) upper portion, guide rail slidable mounting slider (502) are passed through to mounting bracket (501) side, motor (502) output shaft is connected lead screw (503), screw seat (504) are established to lead screw (503) overcoat, screw seat (504) one end is connected slider (502), and the other end passes through the transfer line and connects piston or gasbag.
2. The totally enclosed reaction liquid preparation apparatus according to claim 1, characterized in that: one or more sample adding holes (201) are arranged on the upper cover (200).
3. The totally enclosed reaction liquid preparation apparatus according to claim 1, characterized in that: the outer side of the liquid exchanger (400) vertically penetrates through a communication hole (401), a communication groove (301) is formed in the communicator (300), one end of the communication groove is communicated with the inner cavity of the liquid exchanger (400), and the other end of the communication groove is communicated with the communication hole (401).
4. The totally enclosed reaction liquid preparation apparatus according to claim 3, characterized in that: the communication hole (401) and the reagent cavity bottom hole are on the same diameter.
5. The totally enclosed reaction liquid preparation apparatus according to claim 1, characterized in that: the reagent cavity comprises a sample cavity (101), a diluent cavity (102), a quantitative cavity (104) and a mixing cavity (106); the dosing chamber (104) is adjacent to the waste chamber (110) and may be in communication with each other.
6. The totally enclosed reaction liquid preparation apparatus according to claim 5, characterized in that: the sample chamber (101), the diluent chamber (102), the quantitative chamber (104) and the mixing chamber (106) may be one or more.
7. The totally enclosed reaction liquid preparation apparatus according to claim 5, characterized in that: open sample chamber bottom hole (111) sample chamber (101) bottom, open diluent chamber (102) bottom has diluent chamber bottom hole (112), be equipped with ration chamber bottom hole (105) and ration chamber inlet channel (103) in ration chamber (104), open mixing chamber bottom hole (107) mixing chamber (106) bottom.
8. The totally enclosed reaction liquid preparation apparatus according to claim 7, characterized in that: quantitative chamber bottom outlet (105) are located quantitative chamber (104) bottom, quantitative chamber inlet channel (103) liquid outlet is located quantitative chamber (104) upper portion, quantitative chamber inlet channel (103) income liquid mouth is located quantitative chamber (104) bottom.
9. The totally enclosed reaction liquid preparation apparatus according to claim 1, characterized in that: the communicating vessel (300) and the liquid exchanger (400) are integrally connected.
10. The totally enclosed reaction liquid preparation apparatus according to claim 5, characterized in that: the mixing chamber (106) may be pre-filled with a liquid reagent or a solid lyophilized reagent.
Technical Field
The invention belongs to the technical field of biological experiments, and relates to a fully-closed reaction liquid preparation device.
Background
The preparation of the reaction solution for nucleic acid detection generally requires diluting a nucleic acid sample to a concentration required for detection, and then sucking a certain amount of the nucleic acid sample, a reaction buffer solution, an enzyme solution and a primer solution according to the detection requirement, and uniformly mixing. At present, nucleic acid detection is a common detection method, but the preparation of a nucleic acid detection reaction solution is mainly performed manually, the whole process is finished by professional technicians, and meanwhile, a separate solution preparation laboratory is required to prevent cross contamination, so that the nucleic acid detection time is prolonged, and the popularization and application of a nucleic acid detection technology are limited.
Disclosure of Invention
The invention aims to provide a fully-closed reaction liquid preparation device which can solve the problems.
According to the technical scheme provided by the invention: a fully-closed reaction liquid preparation device comprises a reagent cavity shell, wherein a connecting through hole, a reagent cavity, a waste liquid cavity and a liquid exchanger are arranged on the reagent cavity shell, a reagent cavity bottom hole is formed in the bottom of the reagent cavity, an upper cover is arranged above the reagent cavity, a piston or an air bag is arranged in the liquid exchanger, and the lower portion of the liquid exchanger is connected with a communicating vessel; the slider is driven by drive element, drive element includes the mounting bracket, the vertical installation motor in mounting bracket upper portion, guide rail slidable mounting slider is passed through to the mounting bracket side, motor output shaft connects the lead screw, the screw seat is established to the lead screw overcoat, screw seat one end is connected the slider, the other end passes through the transfer line and connects piston or gasbag.
As a further improvement of the invention, one or more sample adding holes are arranged on the upper cover.
As a further improvement of the invention, the outer side of the liquid exchanger vertically penetrates through a communication hole, a communication groove is formed in the communicator, one end of the communication groove is communicated with an inner cavity of the liquid exchanger, and the other end of the communication groove is communicated with the communication hole.
As a further improvement of the invention, the communication hole and the reagent chamber bottom hole are on the same diameter.
As a further improvement of the invention, the reagent cavity comprises a sample cavity, a diluent cavity, a quantitative cavity and a mixing cavity; the dosing chamber is adjacent to the waste chamber and may be interconnected.
As a further improvement of the present invention, the sample chamber, the diluent chamber, the quantifying chamber and the mixing chamber may be one or more.
As a further improvement of the invention, the bottom of the sample cavity is provided with a sample cavity bottom hole, the bottom of the diluent cavity is provided with a diluent cavity bottom hole, the quantitative cavity is internally provided with a quantitative cavity bottom hole and a quantitative cavity liquid inlet channel, and the bottom of the mixing cavity is provided with a mixing cavity bottom hole.
As a further improvement of the invention, the bottom hole of the quantitative cavity is positioned at the bottom of the quantitative cavity, the liquid outlet of the liquid inlet channel of the quantitative cavity is positioned at the upper part of the quantitative cavity, and the liquid inlet of the liquid inlet channel of the quantitative cavity is positioned at the bottom of the quantitative cavity.
As a further improvement of the invention, the communicating vessel and the liquid exchanger are integrally connected.
As a further improvement of the invention, the mixing cavity can be filled with liquid reagents or solid freeze-drying reagents.
The positive progress effect of this application lies in:
1. the invention has simple structure and small occupied space; the working time is short: the preparation of the reaction liquid can be completed within 2min, and compared with manual operation, the speed can be improved by 5-10 times.
2. The invention is full-automatic, the operation is convenient: sample dilution, volume quantification, reagent mixing and the like are integrated in one card box, various reaction liquid components do not need to be added manually, and the full automation of the whole process is realized.
3. The invention discloses a closed card box: only need add the sample, all other operations are in airtight card box, multiple anti-pollution and prevent leaking reagent, reduce nucleic acid aerosol pollution risk, no test sample and reagent leak risk.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Figure 2 is a top view of a
Fig. 3 is a plan view of the
FIG. 4 is a view showing the structure of the communicating
Detailed Description
The following further describes embodiments of the present invention with reference to the drawings.
In fig. 1 to 4, the reagent chamber housing 100, the
As shown in fig. 1-4, the present invention is a fully-enclosed reaction solution preparing apparatus, including a
The reagent cavity at least comprises a
A connection through
The
The
The
The
The communicating
The
The mixing chamber 106 may be pre-filled with a liquid reagent or a solid lyophilized reagent.
In the invention, different reagents can be pre-filled in the closed reagent cavity, and no leakage can be realized.
The reagent chamber is connected with the inner chamber of the
The piston or the air bag is driven by a driving element 500, the driving element 500 comprises a mounting frame 501, a motor 502 is vertically mounted on the upper portion of the mounting frame 501, a sliding block 502 is slidably mounted on the side surface of the mounting frame 501 through a guide rail, an output shaft of the motor 502 is connected with a lead screw 503, a screw seat 504 is sleeved outside the lead screw 503, one end of the screw seat 504 is connected with the sliding block 502, and the other end of the screw seat is connected with the piston or the.
The mounting bracket 501 is mounted on top of the
The motor 502 drives the nut seat 504 to move up and down through the screw rod 503, so as to realize the ascending or descending of the piston or the air bag.
The driving element can also be a micro air cylinder or a micro oil cylinder.
1. Sample dilution
Rotating the communicating
2. Volume quantification
The communicating
3. Transferring a quantitative solution
The communicating
4. Dissolving freeze-drying reagent
And rotating the communicating
5. Subsequent treatment of the reaction solution
The piston or the air bag ascends to transfer the liquid in the reagent tube to the
In the invention, other reserved cavities are started when needed.
In the invention, different reagents can be pre-filled in the closed reagent cavity, and no leakage can be realized.
In the invention, the inner cavity of the
In the present invention, the fixing of the
In the invention, the subsequent treatment of the reaction solution, such as heating, amplification, detection and the like, can be realized by adding other structures or devices.
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