阅读说明：本技术 一种高纯化的凤梨酵素的天然复合洗涤剂及其制法 (Natural composite detergent of high-purity pineapple enzyme and preparation method thereof ) 是由 訾孟州 于 2020-08-10 设计创作，主要内容包括：本发明涉及天然高分子洗涤剂技术领域,且公开了一种高纯化的凤梨酵素的天然复合洗涤剂,包括以下配方原料及组分：高纯化凤梨酵素、茶枯粉、脂肪醇聚氧乙烯醚硫酸钠,脂肪酸甲酯磺酸钠、柠檬酸、椰油酰羟乙磺酯钠。该一种高纯化的凤梨酵素的天然复合洗涤剂,通过氯化胆碱-柠檬酸体系作为低共熔溶剂,通过磷酸氢二钾水溶液+乙醇的双水相混合溶剂萃取和CO<Sub>2</Sub>超临界萃取,得到高纯化凤梨酵素,以天然高分子高纯度的凤梨酵素和茶枯粉作为表面活性剂和助洗剂,得到然复合洗涤剂对无机盐、蛋白质和脂肪具有优异清洗的效果。(The invention relates to the technical field of natural polymer detergents and discloses a natural composite detergent of high-purity pineapple ferment, which comprises the following formula raw materials and components: high-purification pineapple ferment, tea seed cake powder, fatty alcohol-polyoxyethylene ether sodium sulfate, fatty acid methyl ester sodium sulfonate, citric acid and cocoyl isethionate sodium. The natural compound detergent of the high-purity pineapple ferment takes a choline chloride-citric acid system as a eutectic solvent and phosphoric acidAqueous two-phase mixed solvent extraction of aqueous solution of dipotassium hydrogen and ethanol and CO 2 Performing supercritical extraction to obtain high-purity pineapple ferment, and taking natural high-molecular high-purity pineapple ferment and tea seed cake powder as a surfactant and a builder to obtain the composite detergent with excellent cleaning effect on inorganic salt, protein and fat.)

1. A natural composite detergent of high-purity pineapple ferment is characterized in that: the fertilizer comprises the following raw materials and components, namely high-purity pineapple ferment, tea seed cake powder, fatty alcohol-polyoxyethylene ether sodium sulfate, fatty acid methyl ester sodium sulfonate, citric acid and cocoyl isethionate sodium in a mass ratio of 15-40:30-80:100:40-100:5-20: 5-20.

2. The natural complex detergent of high-purity pineapple ferment as claimed in claim 1, wherein: the preparation method of the high-purity pineapple ferment comprises the following steps:

(1) adding choline chloride and citric acid into a reaction bottle, heating to 40-80 ℃, and uniformly stirring to form a eutectic solvent;

(2) performing secondary fermentation on the fermented pineapple peels and lactobacillus plantarum, wherein the fermentation temperature is 25-35 ℃, and the fermentation time is 24-72 h;

(3) centrifugally separating and purifying the secondary fermentation product in the step (2), grinding the secondary fermentation product into fine powder, placing the fine powder into a double-aqueous-phase mixed solvent of dipotassium hydrogen phosphate aqueous solution and ethanol, placing the fine powder into a constant-temperature ultrasonic instrument, carrying out ultrasonic dispersion treatment, centrifugally separating, extracting a solid product by using the double-aqueous-phase mixed solvent of the dipotassium hydrogen phosphate aqueous solution and the ethanol, taking an ethanol organic phase, carrying out reduced pressure distillation and drying to prepare the pineapple ferment;

(4) adding a double-water-phase mixed solvent of dipotassium hydrogen phosphate aqueous solution and ethanol into the eutectic solvent obtained in the step (1), adding the pineapple enzyme obtained in the step (3), centrifugally separating to obtain an ethanol organic phase, centrifugally washing and purifying, carrying out reduced pressure distillation on the ethanol organic phase, and placing a solid product in supercritical CO₂In an extraction device, distilled water-ethanol is used as an entrainer, the flow rate is 0.3-0.5L/min, and the pineapple ferment is extracted and purified at 30-50 ℃ under the pressure of 20-30MPa to prepare high-purity pineapple ferment;

(5) placing the high-purity pineapple ferment, the tea seed cake powder, the fatty alcohol-polyoxyethylene ether sodium sulfate, the fatty acid methyl ester sodium sulfonate, the citric acid and the cocoyl isethionate sodium into a distilled water solvent, controlling the solid content to be 50-80%, and uniformly stirring to prepare the high-purity natural composite detergent for the pineapple ferment.

3. The natural complex detergent of high-purity pineapple ferment as claimed in claim 2, wherein: the mass ratio of the choline chloride to the citric acid in the step (1) is 15-30: 10.

4. The natural complex detergent of high-purity pineapple ferment as claimed in claim 2, wherein: the mass ratio of the fermented pineapple peels to the lactobacillus plantarum in the step (2) is 100: 0.1-0.5.

5. The natural complex detergent of high-purity pineapple ferment as claimed in claim 2, wherein: the constant-temperature ultrasonic instrument in the step (3) comprises a heat preservation layer, a reaction bottle via hole is formed above the heat preservation layer, an adjusting nut is arranged above the heat preservation layer, the adjusting nut is movably connected with a screw rod, a heat preservation block is fixedly connected with the screw rod, a water bath is arranged inside the heat preservation layer, an object stage is arranged below the water bath, an ultrasonic device is arranged inside the water bath, the ultrasonic treatment frequency is 20-40KHz, and the ultrasonic temperature is 30-60 ℃.

6. The natural complex detergent of high-purity pineapple ferment as claimed in claim 2, wherein: the mass fraction of the dipotassium phosphate aqueous solution in the step (3) is 20-40%, and the volume ratio of the dipotassium phosphate aqueous solution to the ethanol is 10: 10-15.

Technical Field

The invention relates to the technical field of natural polymer detergents, in particular to a natural composite detergent of high-purity pineapple ferment and a preparation method thereof.

Background

The main components of the detergent are surfactant, builder, additive and the like, and the detergent is a product specially prepared for cleaning through a cleaning process and can be divided into heavy-duty detergent and light-duty detergent, such as soap, laundry detergent, soap powder and the like, wherein the main cleaning function of the detergent is synthetic high molecular surfactant, such as zwitterionic surfactant, nonionic surfactant and cationic surfactant, but the surfactants have different degrees of damage to the skin, and a plurality of natural high molecules, such as tea saponin, tea cumin powder and the like, have good cleaning effect.

The pineapple ferment is a natural plant protease, preferentially hydrolyzes peptide chains on carboxyl sides of basic amino acids and aromatic amino acids, can selectively hydrolyze fibrin and decompose muscle fibers, and has wide application prospects in the aspects of inflammation resistance, swelling reduction, detergents and the like, so that the purification of the pineapple ferment and the improvement of the purity of the pineapple ferment are greatly helpful for the cleaning capacity of the detergents.

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a natural composite detergent for cleaning high-efficiency and high-purification pineapple ferment and a preparation method thereof by taking high-purity pineapple ferment as a natural high-molecular surfactant and taking tea seed cake powder as a builder.

(II) technical scheme

In order to achieve the purpose, the invention provides the following technical scheme: a natural composite detergent of high-purity pineapple ferment is characterized in that: the fertilizer comprises the following raw materials and components, namely high-purity pineapple ferment, tea seed cake powder, fatty alcohol-polyoxyethylene ether sodium sulfate, fatty acid methyl ester sodium sulfonate, citric acid and cocoyl isethionate sodium in a mass ratio of 15-40:30-80:100:40-100:5-20: 5-20.

Preferably, the preparation method of the high-purity pineapple ferment comprises the following steps:

(1) adding choline chloride and citric acid into a reaction bottle, heating to 40-80 ℃, and uniformly stirring to form a eutectic solvent.

(2) Performing secondary fermentation on the fermented pineapple peels and lactobacillus plantarum, wherein the fermentation temperature is 25-35 ℃, and the fermentation time is 24-72 h.

(3) And (3) centrifugally separating and purifying the secondary fermentation product in the step (2), grinding the secondary fermentation product into fine powder, placing the fine powder into a double-aqueous-phase mixed solvent of dipotassium hydrogen phosphate aqueous solution and ethanol, placing the fine powder into a constant-temperature ultrasonic instrument, carrying out ultrasonic dispersion treatment, centrifugally separating, extracting a solid product by using the double-aqueous-phase mixed solvent of the dipotassium hydrogen phosphate aqueous solution and the ethanol, taking an ethanol organic phase, carrying out reduced pressure distillation and drying, and preparing the pineapple ferment.

(4) Adding a double-water-phase mixed solvent of dipotassium hydrogen phosphate aqueous solution and ethanol into the eutectic solvent obtained in the step (1), adding the pineapple enzyme obtained in the step (3), centrifugally separating to obtain an ethanol organic phase, centrifugally washing and purifying, carrying out reduced pressure distillation on the ethanol organic phase, and placing a solid product in supercritical CO₂In the extraction device, distilled water-ethanol is used as an entrainer, the flow rate is 0.3-0.5L/min, and the pineapple ferment is extracted and purified at 30-50 ℃ under the pressure of 20-30MPa to prepare the high-purity pineapple ferment.

(5) Placing the high-purity pineapple ferment, the tea seed cake powder, the fatty alcohol-polyoxyethylene ether sodium sulfate, the fatty acid methyl ester sodium sulfonate, the citric acid and the cocoyl isethionate sodium into a distilled water solvent, controlling the solid content to be 50-80%, and uniformly stirring to prepare the high-purity natural composite detergent for the pineapple ferment.

Preferably, the mass ratio of the choline chloride to the citric acid in the step (1) is 15-30: 10.

Preferably, the mass ratio of the fermented pineapple peels to the lactobacillus plantarum in the step (2) is 100: 0.1-0.5.

Preferably, the constant-temperature ultrasonic instrument in the step (3) comprises a heat insulation layer, a reaction bottle via hole is formed in the upper portion of the heat insulation layer, an adjusting nut is arranged on the upper portion of the heat insulation layer, a screw rod is movably connected with the adjusting nut, a heat insulation block is fixedly connected with the screw rod, a water bath is arranged in the heat insulation layer, an object stage is arranged below the water bath, an ultrasonic device is arranged in the water bath, the ultrasonic treatment frequency is 20-40KHz, and the ultrasonic temperature is 30-60 ℃.

Preferably, the mass fraction of the dipotassium phosphate aqueous solution in the step (3) is 20-40%, and the volume ratio of the dipotassium phosphate aqueous solution to the ethanol is 10: 10-15.

(III) advantageous technical effects

Compared with the prior art, the invention has the following beneficial technical effects:

the high-purity pineappleThe natural composite detergent of the ferment is prepared by taking a choline chloride-citric acid system as a eutectic solvent, extracting by using a double-aqueous-phase mixed solvent of a dipotassium hydrogen phosphate aqueous solution and ethanol and CO₂Performing supercritical extraction to obtain high-purity pineapple ferment, and taking natural high-molecular high-purity pineapple ferment and tea seed cake powder as a surfactant and a builder to obtain the composite detergent with excellent cleaning effect on inorganic salt, protein and fat.

Drawings

FIG. 1 is a schematic front view of a constant temperature ultrasound apparatus;

FIG. 2 is a schematic top view of an ultrasound device;

FIG. 3 is an enlarged schematic view of the adjustment nut;

fig. 4 is a schematic view of screw adjustment.

1-constant temperature ultrasonic instrument; 2-insulating layer; 3-passing a reaction bottle hole; 4-adjusting the nut; 5-a screw; 6-heat preservation block; 7-water bath; 8-an object stage; 9-ultrasonic device.

Detailed Description

To achieve the above object, the present invention provides the following embodiments and examples: a natural composite detergent of high-purity pineapple ferment is characterized in that: the fertilizer comprises the following raw materials and components, namely high-purity pineapple ferment, tea seed cake powder, fatty alcohol-polyoxyethylene ether sodium sulfate, fatty acid methyl ester sodium sulfonate, citric acid and cocoyl isethionate sodium in a mass ratio of 15-40:30-80:100:40-100:5-20: 5-20.

The preparation method of the high-purity pineapple ferment comprises the following steps:

(1) adding choline chloride and citric acid into a reaction bottle according to the mass ratio of 15-30:10, heating to 40-80 ℃, and uniformly stirring to form a eutectic solvent.

(2) Performing secondary fermentation on the fermented pineapple peels and lactobacillus plantarum at the fermentation temperature of 25-35 ℃ for 24-72h, wherein the mass ratio of the fermented pineapple peels to the lactobacillus plantarum is 100: 0.1-0.5.

(3) And (3) centrifugally separating and purifying the secondary fermentation product in the step (2), grinding the secondary fermentation product into fine powder, placing the fine powder in a double-water-phase mixed solvent of dipotassium hydrogen phosphate aqueous solution and ethanol, placing the fine powder in a constant-temperature ultrasonic instrument, wherein the constant-temperature ultrasonic instrument comprises a heat insulation layer, a reaction bottle through hole is formed above the heat insulation layer, an adjusting nut is arranged above the heat insulation layer, the adjusting nut is movably connected with a screw, the screw is fixedly connected with a heat insulation block, a water bath is arranged inside the heat insulation layer, a carrying platform is arranged below the water bath, an ultrasonic device is arranged inside the water bath, ultrasonic dispersion treatment is carried out at 30-60 ℃ under 20-40KHz, centrifugal separation is carried out, a solid product is extracted by using the double-water-phase mixed solvent of dipotassium hydrogen.

(4) Adding a double-aqueous-phase mixed solvent of 20-40% by mass of dipotassium hydrogen phosphate aqueous solution and ethanol into the eutectic solvent in the step (1), wherein the volume ratio of the double-aqueous-phase mixed solvent to the ethanol is 10:10-15, adding the pineapple enzyme in the step (3), centrifugally separating, taking an ethanol organic phase, centrifugally washing and purifying, carrying out reduced pressure distillation on the ethanol organic phase, and placing a solid product in supercritical CO₂In the extraction device, distilled water-ethanol is used as an entrainer, the flow rate is 0.3-0.5L/min, and the pineapple ferment is extracted and purified at 30-50 ℃ under the pressure of 20-30MPa to prepare the high-purity pineapple ferment.

(5) Placing the high-purity pineapple ferment, the tea seed cake powder, the fatty alcohol-polyoxyethylene ether sodium sulfate, the fatty acid methyl ester sodium sulfonate, the citric acid and the cocoyl isethionate sodium into a distilled water solvent, controlling the solid content to be 50-80%, and uniformly stirring to prepare the high-purity natural composite detergent for the pineapple ferment.