阅读说明：本技术 一种生物合成纳米金属硫化物的方法 (Method for biologically synthesizing nano metal sulfide ) 是由 刘宏伟 李伟华 于 2020-07-10 设计创作，主要内容包括：本发明涉及微生物应用领域,具体涉及一种生物合成纳米金属硫化物的方法,所述制备方法采用以下装置,所述装置包括反应腔内设置有隔膜,所述隔膜将反应腔分为上反应室和下反应室；所述上反应室设置有第一进料口和第一出料口,所述第一进料口连接设置有第一蠕动泵；所述第一蠕动泵一端连接所述第一进料口,另一端连接设置第一储料器；所述第一出料口连接设置有第一回收器；所述下反应室设置有第二进料口和第二出料口,所述第二进料口连接设置第二蠕动泵；所述第二蠕动泵一端连接所述第二进料口,另一端连接设置第二储料器；所述第二出料口连接设置有第二回收器。本发明有效的降低了重金属离子对硫酸盐还原菌的毒性作用,并有利于提高生产效率。(The invention relates to the field of microorganism application, in particular to a method for biologically synthesizing nano metal sulfide, which adopts the following device, wherein the device comprises a reaction cavity which is internally provided with a diaphragm, and the diaphragm divides the reaction cavity into an upper reaction chamber and a lower reaction chamber; the upper reaction chamber is provided with a first feeding hole and a first discharging hole, and the first feeding hole is connected with a first peristaltic pump; one end of the first peristaltic pump is connected with the first feed inlet, and the other end of the first peristaltic pump is connected with a first material storage device; the first discharge hole is connected with a first recovery device; the lower reaction chamber is provided with a second feeding hole and a second discharging hole, and the second feeding hole is connected with a second peristaltic pump; one end of the second peristaltic pump is connected with the second feed port, and the other end of the second peristaltic pump is connected with a second material storage device; and a second recoverer is connected to the second discharge hole. The invention effectively reduces the toxic effect of heavy metal ions on sulfate reducing bacteria and is beneficial to improving the production efficiency.)

1. The method for biologically synthesizing the nano metal sulfide is characterized in that the preparation method adopts a device which comprises a reaction cavity (1), wherein a diaphragm (2) is arranged in the reaction cavity (1), and the diaphragm (2) divides the reaction cavity (1) into an upper reaction chamber (3) and a lower reaction chamber (4); the upper reaction chamber (3) is provided with a first feeding hole (31) and a first discharging hole (32), and the first feeding hole (31) is connected with a first peristaltic pump (33); one end of the first peristaltic pump (33) is connected with the first feeding hole (31), and the other end of the first peristaltic pump is connected with a first material storage device (34); the first discharge hole (32) is connected with a first recovery device (35); the lower reaction chamber (4) is provided with a second feeding hole (41) and a second discharging hole (42), and the second feeding hole (41) is connected with a second peristaltic pump (43); one end of the second peristaltic pump (43) is connected with the second feeding hole (41), and the other end of the second peristaltic pump is connected with a second material storage tank (44); and a second recovery device (45) is connected and arranged on the second discharge hole (42).

2. The method for biosynthesizing nano-sized metal sulfide as recited in claim 1 comprising the steps of:

s1, respectively placing SRB culture solution in an upper reaction chamber and a first material storage device, and respectively placing metal ion solution in a lower reaction chamber and a second material storage device;

s2, deoxidizing the inside of the upper reaction chamber, the lower reaction chamber, the first stocker and the second stocker;

s3, inoculating an SRB strain in the upper reaction chamber, starting the first peristaltic pump and the second peristaltic pump, and reacting for 1-14 days at the temperature of 30-50 ℃.

3. The method of biosynthesizing nanometal sulfide as in claim 2 wherein said metal ion solution comprises one or more of a cadmium salt, a copper salt or a cobalt salt.

4. The method for biosynthesizing nano metal sulfide as recited in claim 3, wherein the concentration of said metal ion is 100-1000 mg/L.

5. The method for the biosynthesis of a nanometal sulfide as claimed in claim 2, wherein the step S3 is performed by inoculating the metal with 10 of the amount⁴～10⁷cell/mL。

6. The method for biosynthesizing nano metal sulfide as claimed in claim 2, wherein said SRB culture solution comprises 5-15 g/L NaCl and 0.1-0.3 g/L MgSO₄·7H₂O, 0.5-2.0 g/L yeast extract, 0.005-0.02 g/L K₂HPO₄1.0-6.0 ml/L sodium lactate and 0.005-0.02 g/L vitamin C.

Technical Field

The invention relates to the field of microorganism application, in particular to a method for biologically synthesizing a nano metal sulfide.

Technical Field

Since semiconductor nanoparticles have fundamental properties of quantum size effect and dielectric confinement effect,and therefore, exhibit optical, electrical, and photoelectric conversion characteristics different from those of bulk semiconductors. The nanometer metal sulfide has high activity, high quantum efficiency in visible light region and small band gap energy (E)_gApproximately equal to 2.30eV), so the nano metal sulfide has wide application prospect in the aspects of biological markers, energy conversion, sensors, photoelectrocatalysis and the like, and a plurality of achievements have been obtained so far.

The preparation method and the preparation device of the nano metal sulfide are the application foundation and the premise. The main synthesis methods reported in the literature at present include element replacement reaction, pyrolysis reaction, exchange reaction, hydrothermal and solvothermal synthesis, and the like. Each of these methods has advantages and disadvantages. The product obtained by the element replacement method has larger grain diameter, and sulfur steam generated in the preparation process makes the product difficult to maintain the stoichiometric ratio; the pyrolysis reaction requires high temperature, and the obtained product is impure; the exchange reaction for preparing nano metal sulfide is generally applied, but the reaction often adopts H with high toxicity₂S gas is used as a sulfur source; the hydrothermal and solvothermal synthesis method is very effective, and can be used for preparing nano particles with high purity, good crystal form, monodispersity, controllable shape and size easily, and has the defect that a common hydrothermal thermometer is higher. Therefore, the method for preparing the nano metal sulfide, which is simple, economic, green and environment-friendly, is explored and is beneficial to improving the practical application value of the nano metal sulfide.

Sulfate-reducing bacteria (SRB) are a class of microorganisms that have diverse morphologies and nutritional types and that can use sulfates or other oxidized forms of sulfides as electron acceptors to dissimilate organic matter. Widely exists in soil, paddy fields, lakes, marshes, river sediment, sea sediment, petroleum deposits, hot spring water and geothermal regions, even in the oral cavity and intestinal tract of human bodies. In sulfate-rich aerobic and anaerobic environments, sulfate-reducing bacteria are quite active molecules, and sulfate is used as a final electron acceptor to decompose organic matters in water bodies to obtain synthetic cell substances and energy required for life maintenance. Sulfate, which is the final electron acceptor in the metabolic process of sulfate reducing bacteria, is reduced into sulfide ions, which are first accumulated outside cells,then into the cell where the first reaction is SO₄ ^2-Activation of, i.e. SO₄ ^2-Reaction with ATP converts to adenylyl sulfate (APS) and pyrophosphate (PPi), which rapidly decomposes to inorganic phosphate (Pi) driving the reaction to proceed to the left. APS continues to decompose into sulfite and adenosine phosphate (AMP). The sulfite becomes meta-sulfite (S) after dehydration₂O₅ ^2-)，S₂O₅ ^2-Extremely unstable and rapidly transformed into the intermediate product dithionite (S)₂O₄ ^2-)，S₂O₄ ^2-And rapidly converted into S₃O₆ ^2-，S₃O₆ ^2-Decomposition into thiosulphate (S)₂O₃ ^2-) And Sulfite (SO)₃ ^2-)，S₂O₃ ^2-And then is changed into SO through self oxidation-reduction action₃ ^2-And the final product S^2-，S^2-Is expelled from the body into the surrounding environment.

Disclosure of Invention

The invention aims to overcome the technical problems that SRB is easily influenced by heavy metal ions, the activity of the SRB is reduced in the preparation process, continuous operation cannot be realized and the like in the prior art, and provides a device for preparing nano metal sulfide by microorganisms.

Another object of the present invention is to provide the above method for biosynthesis of nano metal sulfide.

The purpose of the invention is realized by the following technical scheme:

a method for biologically synthesizing nano metal sulfide adopts the following device, wherein the device comprises a reaction cavity, a diaphragm is arranged in the reaction cavity, and the diaphragm divides the reaction cavity into an upper reaction chamber and a lower reaction chamber; the upper reaction chamber is provided with a first feeding hole and a first discharging hole, and the first feeding hole is connected with a first peristaltic pump; one end of the first peristaltic pump is connected with the first feed inlet, and the other end of the first peristaltic pump is connected with a first material storage device; the first discharge hole is connected with a first recovery device; the lower reaction chamber is provided with a second feeding hole and a second discharging hole, and the second feeding hole is connected with a second peristaltic pump; one end of the second peristaltic pump is connected with the second feed port, and the other end of the second peristaltic pump is connected with a second material storage device; and a second recoverer is connected to the second discharge hole.

The method for biologically synthesizing the nano metal sulfide comprises the following steps:

s1, respectively placing SRB culture solution in an upper reaction chamber and a first material storage device, and respectively placing metal ion solution in a lower reaction chamber and a second material storage device;

s2, deoxidizing the inside of the upper reaction chamber, the lower reaction chamber, the first stocker and the second stocker;

s3, inoculating an SRB strain in the upper reaction chamber, starting the first peristaltic pump and the second peristaltic pump, and reacting for 1-14 days at the temperature of 30-50 ℃.

Preferably, the metal ion solution includes one or more of a cadmium salt, a copper salt, or a cobalt salt.

Preferably, the concentration of the metal ions is 100-1000 mg/L.

Preferably, in the step S3, the inoculation amount is 10⁴～10⁷cell/mL。

Preferably, the SRB culture solution comprises 5-15 g/L NaCl and 0.1-0.3 g/L MgSO₄·7H₂O, 0.5-2.0 g/L of yeast extract, 0.005-0.02 g/L of K2HPO4, 1.0-6.0 ml/L of sodium lactate, and 0.005-0.02 g/L of vitamin C.

Compared with the prior art, the invention has the following technical effects:

the method for biologically synthesizing the nano metal sulfide provided by the invention utilizes the action of bacterial biological reductase to reduce sulfate into S under natural mild conditions^2-. Produced byS^2-The diaphragm of the semipermeable membrane is combined with heavy metal ions to generate nano metal sulfide, and the waste liquid obtained by reaction is recovered in time through the recoverer, so that the nutrient components in the SRB culture solution are kept, the concentration requirement of the heavy metal ions is also ensured, and the continuous reaction is ensured. The invention effectively reduces the toxic effect of heavy metal ions on sulfate reducing bacteria. The sulfate reducing bacteria are wide in distribution, simple and convenient to culture, simple in process for preparing the nano metal sulfide by using organisms, mild in condition, environment-friendly and suitable for low-cost, large-scale and green production needs. The nano metal sulfide obtained by the method can be applied to biological monitoring, photoelectrocatalysis, adsorption materials and the like, and can obviously improve the efficiency of photoelectrocatalysis and photoelectric conversion by compounding with other matrixes.

Drawings

FIG. 1 is a schematic view of an apparatus for synthesizing nano-sized metal sulfide according to the present invention;

FIG. 2 is a UV spectrum of nano metal sulfide obtained by the method of the present invention;

FIG. 3 is an X-ray diffraction pattern of a nano-metal sulfide obtained by the process of the present invention;

FIG. 4 is a scanning electron microscope image of the nano metal sulfide obtained by the method of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail with reference to specific embodiments. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.

Unless otherwise specified, the devices used in this example are all conventional experimental devices, the materials and reagents used are commercially available, and the experimental methods without specific descriptions are also conventional experimental methods.