阅读说明：本技术 反刍动物过瘤胃的生物活性添加剂及其制备工艺和饲料 (Bioactive additive for rumen bypass of ruminant, preparation process and feed thereof ) 是由 危岩 于伟娜 傅祖显 于 2020-07-08 设计创作，主要内容包括：本发明公开了反刍动物过瘤胃的生物活性添加剂及其制备工艺和饲料,包括将熔融载体置于反应器中进行70-85℃加热溶解,制备载体熔融液；将生物活性物质加入载体熔融液中,搅拌均质2-3h,获得均质熔融液,其中,生物活性物质与熔融载体的重量比取值为0.1-0.7；将3:1比例的Si(OEt)<Sub>4</Sub>和MeSi(OEt)<Sub>3</Sub>在PH＝1的条件下水解获得的硅烷凝胶与均质熔融液混合均匀,利用喷雾干燥处理,获得生物活性添加剂,其中,硅烷凝胶与均质熔融液的重量比值大于0.1。还包括利用以上制备工艺制成的生物活性添加剂,以解决分散生物活性物质方法在较高水溶性的情况下,生物活性物质无法变现出充分的过瘤胃效应的问题和防护剂涂覆生物活性物质方法步骤繁琐且成本高的问题。(The invention discloses a bioactive additive for rumen bypass of ruminant, a preparation process and feed thereof, which comprises the steps of putting a molten carrier into a reactor for heating and dissolving at 70-85 ℃ to prepare a carrier molten liquid; adding bioactive substances into the carrier molten liquid, stirring and homogenizing for 2-3h to obtain homogenized molten liquid, wherein the weight ratio of the bioactive substances to the molten carrier is 0.1-0.7; mixing Si (OEt) in a ratio of 3:1 4 And MeSi (OEt) 3 And (3) uniformly mixing silane gel obtained by hydrolysis under the condition of pH1 with the homogeneous molten liquid, and performing spray drying treatment to obtain the bioactive additive, wherein the weight ratio of the silane gel to the homogeneous molten liquid is more than 0.1. The preparation method also comprises a bioactive additive prepared by the preparation process, so as to solve the problems that the bioactive substance cannot show sufficient rumen bypass effect under the condition of high water solubility in the bioactive substance dispersing method and the problems that the steps of the bioactive substance coating method by the protective agent are complicated and the cost is high.)

1. A preparation process of a bioactive additive for rumen bypass of ruminants is characterized by comprising the following steps:

s1: putting the molten carrier into a reactor, heating and dissolving at 70-85 ℃ to prepare carrier molten liquid;

s2: adding bioactive substances into the carrier molten liquid, and stirring and homogenizing for 2-3h to obtain homogenized molten liquid, wherein the weight ratio of the bioactive substances to the molten carrier is 0.1-0.7;

s3: mixing Si (OEt) in a ratio of 3:1₄And MeSi (OEt)₃And (2) uniformly mixing silane gel obtained by hydrolysis under the condition of pH1 with the homogeneous molten liquid, and performing spray drying treatment to obtain the bioactive additive, wherein the weight ratio of the silane gel to the homogeneous molten liquid is more than 0.1.

2. The process of claim 1, further comprising a step of sieving and packaging the bioactive additive after step S3, wherein the bioactive additive with a particle size of 10-150 mesh is metered and packaged by a vibrating sieve.

3. The process for preparing a bioactive additive for the rumen bypass of ruminants according to claim 1, wherein the molten carrier comprises one or a combination of hardened animal fat, hardened vegetable oil, salt and aliphatic monobasic acid.

4. The process for preparing the bioactive additive for rumen bypass of ruminants according to claim 3, wherein the molten carrier further comprises lecithin and a preservative, the weight ratio of lecithin to the molten carrier is 1-4%, and the weight ratio of the preservative to the molten carrier is 0.01-2%.

5. The process of claim 1, wherein the stirring and homogenizing in step S2 is performed by a homogenizer, wherein the homogenizer has a rotation speed of 1200-.

6. The process for preparing a bioactive additive for the rumen bypass of ruminants according to claim 1, wherein the average particle size of the bioactive substance is 10-150 μm.

7. The process for preparing a bioactive additive for the rumen bypass of ruminants according to claim 1, wherein the bioactive substance comprises one or a combination of amino acids, vitamins, enzymes, proteins, carbohydrates, natural substances and drugs.

8. The process for preparing a bioactive additive for the rumen bypass of ruminants according to claim 1, wherein the spray-drying process in the step S3 is operated in a cooling chamber of a spray-dryer at a temperature of 5-10 ℃.

9. A bioactive additive for rumen bypass of ruminant, wherein the bioactive additive is prepared by the preparation process of the bioactive additive for ruminant rumen bypass according to any one of claims 1-8.

10. A feed, characterized in that the bioactive additive and feed raw materials in the claim 9 are put into a belt mixer according to the proportion of more than 1:50 to be mixed evenly to prepare the bioactive additive feed, wherein the feed raw materials comprise one or the combination of defatted rice bran, wheat flour, dehydrated bean curd residue, corn rice flour and fish meal.

Technical Field

The invention belongs to the field of industrial feed for animal husbandry, and particularly relates to a bioactive additive for rumen bypass of ruminants, a preparation process thereof and feed.

Background

Due to the limited content of bioactive substances in bioactive additives, the animal husbandry and other interested parties have been eager to obtain such bioactive additives because they contain a greater variety of amino acids, vitamins, etc. For the bioactive additive prepared by granulating the bioactive substance dispersed in the protective agent, when the substance content is 40% or less, the bioactive substance with low water solubility can be obtained as an effective bioactive additive. However, if the bioactive substance is a bioactive substance having high water solubility such as lysine hydrochloride, the bioactive substance is not an effective bioactive additive even if the content of the bioactive substance is 40% or less because the rumen-bypass property is less remarkable. The rumen products currently on the market are not sufficiently protective against bioactive substances in the rumen where microorganisms are present. The reason is due to the influence of microorganisms contained in the characteristic rumen of ruminants. However, the presence of microorganisms in the rumen is a fundamental source of energy to sustain ruminant life, and microorganisms are also a source of nutrients such as proteins. Therefore, the developed bioactive additive for ruminants must be coated with a coating ingredient to protect the bioactive substances without affecting the lifespan of microorganisms in the rumen. Although many suggestions have been made on how to stably protect bioactive substances in the rumen of ruminants and promote their release in the abomasum and/or the subsequent digestive tract for coating compositions of bioactive additives for ruminants, bioactive additives that simultaneously have protective capabilities in the rumen and are releasable in the abomasum and/or the subsequent digestive tract are still under investigation. The reason is recognized that, at present, no sufficient research has been conducted on the evaluation methods of the rumen protective ability of ruminants, the particle size of bioactive additives, the coating structure, and the like. Although the simulated rumen test table in the laboratory shows high protective ability, when the product is measured by using real rumen fluid of ruminant in which microorganisms exist, the commercial bioactive additive has no protective ability to a great extent. The reason for this problem is attributed to the influence of a large number of microorganisms in the rumen of ruminants and the bioactive substance is coated with a coating composition capable of inhibiting this influence.

At present, two methods of dispersing bioactive substances and coating the bioactive substances with protective agents are generally adopted to prepare rumen products. However, when the bioactive additive is prepared by dispersing the bioactive substance, the bioactive substance cannot exhibit a sufficient rumen bypass effect in the case of having high water solubility. Although the method of coating a bioactive substance with a protectant can exhibit the characteristics associated with bioactive additives, it has a problem of high cost due to the complicated manufacturing process, and in particular, in the extrusion granulation process, the extrusion under high temperature and pressure conditions causes the decomposition of bioactive substances (such as vitamins susceptible to thermal damage), and the manufacturing equipment is expensive. In addition, the granular bioactive additive obtained by extrusion granulation is generally cylindrical or prismatic, which may cause the bioactive substance in the edge portion to be very easily eluted, fail to satisfy the performance requirements of the bioactive additive, and the granules having the protruding portion such as the edge are also easily abraded by the inner wall of the rumen of the ruminant such as cattle, thereby causing a problem that the bioactive substance is eluted in advance by the peeled coating.

Disclosure of Invention

The application aims to provide a bioactive additive for rumen bypass of ruminants, a preparation process thereof and a feed, so as to solve the problems that a bioactive substance cannot show a sufficient rumen bypass effect under the condition of high water solubility in a dispersing bioactive substance method and the problems that a method for coating the bioactive substance by a protective agent has complicated steps and is high in cost.

According to a first aspect of the present invention, embodiments of the present application provide a process for preparing a bioactive additive for rumen bypass of a ruminant, comprising:

s1: putting the molten carrier into a reactor, heating and dissolving at 70-85 ℃ to prepare carrier molten liquid;

s2: adding bioactive substances into the carrier molten liquid, stirring and homogenizing for 2-3h to obtain homogenized molten liquid, wherein the weight ratio of the bioactive substances to the molten carrier is 0.1-0.7;

s3: mixing Si (OEt) in a ratio of 3:1₄And MeSi (OEt)₃And (3) uniformly mixing silane gel obtained by hydrolysis under the condition of pH1 with the homogeneous molten liquid, and performing spray drying treatment to obtain the bioactive additive, wherein the weight ratio of the silane gel to the homogeneous molten liquid is more than 0.1.

In the method, bioactive substances are dispersed and dissolved in carrier molten liquid at 70-85 deg.C, homogenized for 2-3 hr, and 10 wt% of silane Si (OEt)₄And MeSi (OEt)₃Mixing gel solution hydrolyzed with water at a ratio of 3:1 at pH1, spray drying in a spray dryer, and sieving to obtain 10-150 mesh product as rumen-bypass bioactive additive for ruminant.

Further, after step S3, a sieving package is included, and the bioactive additive with a particle size of 10-150 mesh is metered and packaged by using a vibrating sieve. The bioactive additive with a certain particle size is screened and filtered by utilizing the pore size of the vibrating screen to be packaged as much as possible, so that the feed can be conveniently proportioned and sold in the later period.

Further, the molten carrier comprises one or a combination of hardened animal fat, hardened vegetable oil, salt and aliphatic monobasic acid. The matching requirements of feeds with different processes and flavors are met by selecting materials of various melting carriers.

Furthermore, the melting carrier also comprises lecithin and a preservative, wherein the weight ratio of the lecithin to the melting carrier is 1-4%, and the weight ratio of the preservative to the melting carrier is 0.01-2%. Lecithin is used for controlled release in the subsequent wrinkled stomach and/or small intestine, and the preservative is used for inhibiting the growth of mould, bacteria and the like or has an antibacterial effect.

Further, the stirring and homogenizing in step S2 is processed by a homogenizer, wherein the parameters of the homogenizer are set to a rotation speed of 1200-1500r/min, and the processing time is 2-3 h. And (3) fully and uniformly mixing the bioactive substances and the carrier molten liquid by adopting a homogenizer.

Further, the bioactive substance has an average particle size of 10-150 μm. The average particle size of the biological activity is too large to be exposed to degradation, and too small to increase the viscosity of the injection melt, so that the spray drying process is not easy to be carried out.

Further, the bioactive substance includes one or a combination of amino acids, vitamins, enzymes, proteins, carbohydrates, natural substances, and drugs. The multiple selection of bioactive substances facilitates the replenishment of configuring relevant bioactive substances for different desired species of different ruminants.

Further, the spray drying process in step S3 is operated in a cooling chamber of a spray dryer at a temperature of 5 ℃ to 10 ℃. The spray dryer can rapidly and effectively dry the homogeneous solution, and the formed bioactive additive is spherical while ensuring the activity of the bioactive substance, thereby preventing the risk of premature exposure of the bioactive substance in rumen.

According to the second aspect of the present invention, the embodiment of the present invention also provides a bioactive additive for ruminant rumen bypass, which is prepared by the above preparation process of the bioactive additive for ruminant rumen bypass.

According to a third aspect of the present invention, embodiments of the present invention provide a feed, wherein the bioactive additive prepared by the above preparation process of the bioactive additive for rumen bypass of ruminant animals and feed raw materials are put into a belt mixer according to a ratio of more than 1:50 and mixed uniformly to prepare the bioactive additive feed, wherein the feed raw materials comprise one or a combination of defatted rice bran, wheat flour, dehydrated bean curd residue, corn rice flour and fish meal.

The process for preparing the bioactive additive for rumen bypass of ruminant has excellent rumen bypass property of bioactive substances, even when the preparation contains bioactive substances with high water solubility, the bioactive substances are prepared by granulating in a mode of dispersing in the protective agent. And also has excellent releasability in the abomasum and/or the subsequent digestive tract, while providing a feed containing such a feed additive ingredient for ruminants, and a method of producing such a feed additive ingredient for ruminants. Compared with the prior art, the bioactive additive for rumen bypass of ruminants, which is produced by the preparation process, has high rumen bypass performance of over 90 percent, regular product shape, narrow particle size distribution, easy addition and premixing and high product qualification rate. The addition of the silane gel liquid can increase the rumen bypass rate of the feed additive and is easy to be slowly absorbed in the abomasum and the small intestine. The production process of the bioactive additive feed for ruminant rumen bypass is simple, the production cost is low, the continuous production is easy, no organic solvent is used, and the feed has no pollution to the environment.

Drawings

The accompanying drawings are included to provide a further understanding of the embodiments and are incorporated in and constitute a part of this specification. The drawings illustrate embodiments and together with the description serve to explain the principles of the invention. Other embodiments and many of the intended advantages of embodiments will be readily appreciated as they become better understood by reference to the following detailed description. The elements of the drawings are not necessarily to scale relative to each other. Like reference numerals designate corresponding similar parts.

Fig. 1 is a block diagram of a process for preparing a bioactive additive for rumen bypass of ruminants according to an embodiment of the present invention;

fig. 2 is a flow chart of a process for preparing a bioactive additive for rumen bypass of ruminants according to an embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the present invention will be described in further detail with reference to the accompanying drawings, and it is apparent that the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, fig. 1 is a block diagram illustrating a process for preparing a bioactive additive for ruminant rumen bypass according to a specific embodiment of the present invention. As shown in fig. 1, the preparation process comprises the following steps:

step 101: and (3) putting the molten carrier into a reactor, heating and dissolving at 70-85 ℃ to prepare carrier molten liquid.

In the method, a carrier melt constituting the biologically active substance is prepared, the carrier melt comprising at least one substance selected from the group consisting of hardened animal fat, hardened vegetable oil and wax; lecithin; and a linear or branched, saturated or unsaturated aliphatic monocarboxylic acid or salt having 12 to 22 carbon atoms (used alone or in the form of a mixture of two or more) are placed in a reaction vessel and heated to a temperature adjusted to 70 to 85 ℃, and the setting of the specific heating temperature can be adjusted depending on the composition of the carrier melt and the process cost. Wherein the weight ratio of lecithin to molten carrier is 1% -4%, and the content of lecithin in the molten carrier is controlled to be 0.1-10%, preferably 0.5-7%, and most preferably 0.5-5.0% by weight, for releasing bioactive substances in abomasum and/or subsequent digestive tract. The bioactive additive may suitably comprise lecithin. Lecithin may act as a release-controlling agent in the abomasum and/or the subsequent digestive tract. Furthermore, by adding stearic acid, the bioactive additive can be made to have excellent protective ability in the rumen and to be releasable in the abomasum and/or subsequent digestive tract.

In particular embodiments, hardened animal fat components such as tallow, lard, and the like; hardened vegetable oil ingredients such as hardened palm oil, hardened soybean oil, hardened rapeseed oil, hardened castor oil, and the like; various straight or branched, saturated or unsaturated aliphatic monocarboxylic acids having 12 to 22 carbon atoms, such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, etc.; various fatty acid esters such as monoesters or diesters of saturated or unsaturated fatty acids with glycerin, such as glycerin fatty acid esters, sucrose fatty acid esters, and the like; it is also possible to use carnauba wax, beeswax, natural wax, synthetic wax, paraffin wax or the like (these components may be used alone or in a mixture of two or more). The amount of the composition should be controlled to 20-98 wt%, preferably 30-90 wt%. Various straight or branched chain, saturated or unsaturated aliphatic mono-acids and salts having 12 to 22 carbon atoms, such as myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, sorbic acid or salts and the like (used alone or in mixtures of two or more) may be used. The amount of the composition should be controlled to 0.1-50 wt%, preferably 1-40 wt%.

Preferably, a preservative may also be added for inhibiting the growth of molds, bacteria, etc., or an antibacterial effect, and, conceivably, an antifungal agent, etc., may also be used for this purpose. When the preservative is wrapped in the bioactive additive, the preservative can reduce the invasion and decomposition of rumen microorganisms to bioactive substances and the invasion near the surface of the bioactive additive as much as possible, thereby playing a role in preventing the decomposition. In particular, when a preservative is mixed and dispersed in a carrier melt coated with a bioactive substance, such a preservative can effectively exert its preservative function without damaging the bioactive substance. As for the addition amount of the preservative, excessive addition may affect the propagation and growth of microorganisms, while insufficient addition may not achieve the intended effect. Thus, the weight ratio of preservative to molten carrier is 0.01-2.0%, most preferably 0.1-1.0%, based on the total amount of bioactive additive. The preservative is preferably in the form of a fine powder. Similarly, the preservative may be pulverized as necessary. When the preservative is dispersed in the carrier melt, the feed additive can be prevented from spoiling during storage. The antiseptic can be selected from at least one of propionic acid or salt, sorbic acid or salt, benzoic acid or salt, dehydroacetic acid or salt, p-hydroxybenzoate, imazalil, thiabendazole, o-phenylphenol, sodium o-phenylphenol and biphenyl; various propionic acids or salts can be used, such as propionic acid, calcium propionate, sodium propionate, etc.; various sorbic acids or salts, such as sorbic acid, potassium sorbate, etc., can be used; various benzoic acids and salts may be used, such as benzoic acid, sodium benzoate, and the like; dehydroacetic acid or salts such as dehydroacetic acid, sodium dehydroacetate, and the like; various parabens may also be used, such as isobutyl paraben, isopropyl paraben, ethyl paraben, butyl paraben, propyl paraben, and the like.

Step 102: adding bioactive substances into the carrier molten liquid, stirring and homogenizing for 2-3h to obtain homogenized molten liquid, wherein the weight ratio of the bioactive substances to the molten carrier is 0.1-0.7.

In the method, bioactive substances are dispersed and/or dissolved in carrier molten liquid and are treated by a homogenizer, the parameter of the homogenizer is set as the rotating speed of 1200-1500r/min, the treatment time is 2-3h, the weight ratio of the bioactive substances to the molten carrier is 0.1-0.7, and the bioactive substances comprise one or the combination of amino acid, vitamin, enzyme, protein, carbohydrate, natural substances and medicines.

In particular embodiments, the bioactive substances may include various known nutrients and feeds containing such nutrients or raw materials, such as amino acids, vitamins, enzymes, proteins, carbohydrates, natural substances, and drugs, which may be used alone or in a mixture of two or more. In particular, highly water-soluble bioactive substances such as lysine hydrochloride, betaine, taurine and/or water-soluble vitamins can also be added into the bioactive additive for remarkably enhancing the digestion and absorption capacity. The bioactive substances include various amino acids such as glycine, alanine, arginine, lysine (lysine hydrochloride), glutamic acid, methionine, tryptophan, threonine, valine, sweet amino acid, taurine, etc. (all belonging to amino acids and/or derivatives thereof); various vitamins such as vitamin C, vitamin B1, vitamin B2, vitamin B6, vitamin B12, choline chloride, calcium pantothenate, nicotinic acid amide, biotin, folic acid, p-aminobenzoic acid, etc. (all of which are water-soluble vitamins and/or other substances having corresponding functions); various enzymes such as protease, amylase, lipase, mixed enzyme, etc.; various proteins, such as casein, zein, and the like; various carbohydrates, such as starch, sucrose, glucose, and the like; various natural products, such as fish meal, kelp powder, blood powder, grain powder, bile powder, etc.; various drugs, such as antibiotic drugs, hormonal drugs, and the like; the antibiotic drug further includes aminoglycoside kanamycin sulfate, glycopeptide vancomycin, tetracycline oxytetracycline, and the like; ampicillin and the like for penicillins, erythromycin and the like for macrolides, lincomycin and the like for lincomycin, chloramphenicol group and fosfomycin group; the hormone medicine further comprises estrogen, stilbestrol, caprolactone, etc.

Preferably, the content of biologically active substance should be 1-50% by weight, preferably 20-50% by weight, most preferably 30-45% by weight. In particular, injection is made difficult in the case of injection granulation, since an increased content of water-soluble biologically active substances leads to higher viscosities. In order to meet the requirement that the bioactive additive contains a higher proportion of bioactive substances, the addition amount is determined according to the characteristics of the bioactive substances, and the spray drying treatment is ensured to achieve proper balance. In addition, the bioactive additive may also suitably contain fat-soluble vitamins such as vitamin a, vitamin D, vitamin E, and the like. The specific gravity of the carrier melt can be adjusted by adding a specific gravity controlling agent such as calcium carbonate or talc as a component of the carrier melt.

Further preferably, the method of obtaining a fine powder bioactive substance may use a pin mill, a ball mill or a jet mill for pulverization, thereby limiting the average particle size to less than 150 μm to prepare a suitable bioactive additive. The ratio of bioactive substances with different average particle sizes to rumen bypass rate and bioactive substance exposure is shown in table 1.

TABLE 1

As can be seen from the table, as the average particle size of the bioactive substance increases, the rumen bypass rate decreases gradually and the exposure ratio increases gradually, and further, if the bioactive substance is pulverized to have an average particle size of less than 1 μm dispersed in the melt blend, spray drying becomes difficult due to a corresponding increase in the viscosity of the injection melt. If the average particle diameter is larger than 125 μm, it is difficult to completely coat the surface of the bioactive substance with the carrier melt, thus resulting in partial exposure. Therefore, the average particle size of the bioactive substance must be controlled to be between 10 and 150 micrometers, preferably 10 to 100 micrometers.

Step 103: mixing Si (OEt) in a ratio of 3:1₄And MeSi (OEt)₃And (3) uniformly mixing silane gel obtained by hydrolysis under the condition of pH1 with the homogeneous molten liquid, and performing spray drying treatment to obtain the bioactive additive, wherein the weight ratio of the silane gel to the homogeneous molten liquid is more than 0.1.

In this process, 10% by weight of silane tetraethoxysilane Si (OEt)₄And triethoxytetrasilane MeSi (OEt)₃Mixing with gel solution hydrolyzed with appropriate amount of water at pH1 at a ratio of 3:1, mixing well to obtain spray-dried injection, and spray granulating the injection in a spray dryer at 70-85 deg.C. Since granulation is carried out by mixing the carrier melt with the biologically active substance and then injecting air, fewer manufacturing steps are required than in extrusion granulation, and it is therefore easier to performThe bioactive additive is obtained at lower cost. The addition of the silane gel solution can increase rumen bypass rate of the bioactive additive, and is easy to be slowly absorbed in the abomasum and small intestine. Furthermore, the bioactive substance can be dispersed by uniformly stirring and homogenizing in the carrier melt to obtain a spherical shape and the bioactive additive having a selected particle size. Making the manufacture of high quality bioactive additives easier and less costly.

In a particular embodiment, the bioactive additive prepared to form the rumen bypass of ruminants should be spherical in shape and have an average particle size of 0.5 mm to 3.0 mm. The smaller the particle size of the formulation, the shorter the residence time in the rumen and the better the digestion, and furthermore, the greater the friction between the bioactive additive and the rumen wall and the higher the release rate. Therefore, the particle size is preferably 0.5 mm to 2.0 mm. As for the particle size of the bioactive substance, a smaller particle size will reduce the amount of bioactive substance present on or near the surface of the spherical bioactive additive, making the mixing of the bioactive substance with the carrier melt more uniform, however, since the particle size of the bioactive substance is related to the ease of spray drying. Therefore, the average particle size of the bioactive substance should be controlled between 10-150 μm. The particle size of the specific bioactive additive is shown in table 2 in relation to the rumen release rate, abomasum release rate and subsequent release rate in the digestive tract.

Table 2:

in a specific embodiment, the bioactive additive prepared by the preparation process of the bioactive additive in steps 101-103 and feed raw materials are put into a belt mixer according to a ratio of more than 1:50 and are uniformly mixed to prepare the bioactive additive feed, wherein the feed raw materials comprise one or a combination of defatted rice bran, wheat flour, dehydrated bean curd residue, corn rice flour and fish meal.

Referring to fig. 2, fig. 2 illustrates a flow chart of a process for preparing a bioactive additive for ruminant rumen bypass according to a specific embodiment of the present invention. As shown in FIG. 2, the preparation process comprises the steps of preparing a carrier melt 201, homogenizing 202, preparing a spray injection 203, sieving and sorting 204, and the specific flow is as follows.

Step 201: a carrier melt is prepared. Heating the reaction kettle, controlling the temperature at 70-85 ℃, wherein the molten carrier contains: at least one of hardened rapeseed oil and hardened palm oil; stearic acid, lecithin, etc., are used to prepare the carrier melt by dispersing or dissolving the amino acids in the melt.

Step 202: and (4) homogenizing. After the feeding of the bioactive substances is finished, the rotating speed of a homogenizer is set to be 1200-1500r/min, and the materials obtained by the treatment of the carrier molten liquid added with the bioactive substances are stirred and homogenized for 2-3 hours.

Step 203: spray injection is prepared. Then adding 10 wt% of silane gel liquid, wherein Si (OEt)₄And MeSi (OEt)₃The gel solution hydrolyzed with water at a ratio of 3:1 at pH1 is mixed with the homogeneous melt obtained in step 202 to form a spray-dried injection.

Step 204: sieving, sorting and packaging. Collecting materials with the particle size of 10-150 meshes by using a vibrating screen, and carrying out metering, weighing and packaging to obtain the rumen-bypass bioactive additive for the ruminant.