阅读说明：本技术 一种含细胞提取液的护肤精华液及其制备方法 (Skin care essence containing cell extracting solution and preparation method thereof ) 是由 胡以亮 于 2020-06-29 设计创作，主要内容包括：本发明公开了一种含细胞提取液的护肤精华液及其制备方法,该护肤精华液包括以下重量份的组分：紫甘薯细胞提取液10-15份、人参提取物0.5-1份、牡丹根提取物0.25-0.5份、桑叶提取物0.08-0.2份、银耳多糖2-4份、烟酰胺0.4-0.6份、生育酚0.2-0.4份、海藻糖0.5-1.0份、甜菜碱0.5-1.5份、甘油2-5份、双丙甘醇3-5份、增稠剂0.4-0.5份、三乙醇胺0.25-0.4份、螯合剂0.08-0.12份、水65-80份；本发明以紫甘薯细胞提取液、人参提取物、牡丹根提取物、桑叶提取物为主料,辅以保湿剂、增稠剂等,合理配伍,科学配比,使不同的有效活性成分产生协同增效作用,赋予产品优异的清除羟自由基、增强皮肤弹性、延缓衰老、补充肌肤水分的功效,且制备工艺简单,适应工业化生产。(The invention discloses a skin care essence containing a cell extracting solution and a preparation method thereof, wherein the skin care essence comprises the following components in parts by weight: 10-15 parts of purple sweet potato cell extracting solution, 0.5-1 part of ginseng extract, 0.25-0.5 part of peony root extract, 0.08-0.2 part of mulberry leaf extract, 2-4 parts of tremella polysaccharide, 0.4-0.6 part of nicotinamide, 0.2-0.4 part of tocopherol, 0.5-1.0 part of trehalose, 0.5-1.5 parts of betaine, 2-5 parts of glycerol, 3-5 parts of dipropylene glycol, 0.4-0.5 part of thickening agent, 0.25-0.4 part of triethanolamine, 0.08-0.12 part of chelating agent and 65-80 parts of water; the invention takes the purple sweet potato cell extract, the ginseng extract, the peony root extract and the mulberry leaf extract as main materials, and is supplemented with the humectant, the thickener and the like, and the compatibility and the scientific proportion are reasonable, so that different effective active ingredients generate the synergistic interaction effect, the product has excellent effects of removing hydroxyl free radicals, enhancing skin elasticity, delaying senility and supplementing skin moisture, and the preparation process is simple and is suitable for industrial production.)

1. A skin care essence containing cell extract is characterized in that: the paint comprises the following components in parts by weight: 10-15 parts of purple sweet potato cell extract, 2-4 parts of ginseng extract, 0.5-1 part of peony root extract, 0.25-0.5 part of mulberry leaf extract, 0.08-0.2 part of tremella polysaccharide, 0.4-0.6 part of nicotinamide, 0.2-0.4 part of tocopherol, 0.5-1.0 part of trehalose, 0.5-1.5 parts of betaine, 2-5 parts of glycerol, 3-5 parts of dipropylene glycol, 0.4-0.5 part of thickener, 0.25-0.4 part of triethanolamine, 0.08-0.12 part of chelating agent and 65-80 parts of water.

2. The skin care essence containing cell extract according to claim 1, wherein: the skin care essence containing the cell extracting solution comprises the following components in parts by weight: 12 parts of purple sweet potato cell extracting solution, 3 parts of ginseng extract, 0.8 part of peony root extract, 0.4 part of mulberry leaf extract, 0.15 part of tremella polysaccharide, 0.5 part of nicotinamide, 0.3 part of tocopherol, 0.8 part of trehalose, 1.15 parts of betaine, 4 parts of glycerol, 4 parts of dipropylene glycol, 0.45 part of thickening agent, 0.3 part of triethanolamine, 0.1 part of chelating agent and 78 parts of water.

3. The skin care essence containing cell extract according to claim 1, wherein: the thickening agent is one or more of carbomer and xanthan gum.

4. The skin care essence containing cell extract according to claim 1, wherein: the chelating agent is disodium EDTA.

5. The skin care essence containing cell extract according to claim 1, wherein: the water is pure water with the conductivity less than or equal to 2 mu S/cm.

6. The skin care essence containing cell extract according to claim 1, wherein: the skin care essence containing the cell extracting solution further comprises one or more of an aromatic, a toner and a preservative.

7. The method for preparing the skin care essence containing the cell extract according to any one of claims 1 to 6, comprising the steps of:

(1) centrifuging the purple sweet potato cell culture solution, removing the precipitate, freezing and crushing the supernatant at ultralow temperature by using liquid nitrogen to obtain purple sweet potato cell powder;

(2) taking a proper amount of purple sweet potato cell powder, adding 50mmol/L phosphate buffer solution with pH of 7.0 to obtain purple sweet potato cell sap with the concentration of 20mg/mL, repeatedly freezing and thawing for five times at-80 ℃, centrifuging at 3-5 ℃, taking supernate, namely purple sweet potato cell extracting solution, and storing at 3-5 ℃ for later use;

(3) adding accurately weighed water, glycerol, betaine, trehalose, dipropylene glycol, thickening agent and chelating agent into an emulsifying pot, stirring, heating to 85-90 deg.C, stirring, and sterilizing at constant temperature for 20-25 min;

(4) starting circulating cooling water, continuing stirring, cooling the temperature in the emulsifying pot to 42-48 deg.C, adding accurately weighed purple sweet potato cell extractive solution, Ginseng radix extract, peony root extract, folium Mori extract, Tremella polysaccharide, nicotinamide, tocopherol and triethanolamine, and stirring;

(5) and starting circulating cooling water, continuing stirring until the temperature of the material is reduced to 38 ℃, stopping stirring after the pH value is qualified, and discharging.

8. The method for preparing the skin care essence containing the cell extract according to claim 7, wherein the extraction method of the purple sweet potato cell culture solution is as follows: taking fresh and non-rotten purple sweet potatoes as a raw material, cleaning, cutting into blocks, cooking, pulping with water according to the weight ratio of 1: 1.5-2.5, and then carrying out enzymolysis by adopting alpha-amylase and glucoamylase at the temperature of 50-70 ℃ for 60-120min, wherein the addition amount of the alpha-amylase is 0.01-0.09% of the weight of the purple sweet potato pulp raw material, and the addition amount of the glucoamylase is 0.01-0.05% of the weight of the purple sweet potato pulp raw material, so as to prepare a purple sweet potato saccharification liquid; inoculating 6-12% yeast seed culture solution at volume ratio, culturing at 25-30 deg.C for 3-4 days with pH of 3.0-3.5, and filtering after fermentation.

9. The method for preparing skin care essence containing cell extract according to claim 7, wherein in the step (1), the centrifugation conditions are as follows: the rotating speed is 3000-.

10. The method for preparing skin care essence containing cell extract according to claim 7, wherein in the step (2), the centrifugation conditions are as follows: the rotating speed is 8000-.

Technical Field

The invention relates to a skin care essence, in particular to a skin care essence containing a cell extracting solution and a preparation method thereof, and belongs to the technical field of cosmetics.

Background

With the age, the water loss in the skin is obvious, the water and oil are unbalanced, and the problems of acne, roughness, wrinkles and the like are easy to occur; meanwhile, the gradually deteriorating environment increases the content of pollutants in the air, and the skin is exposed to the air for a long time and is damaged by the pollutants in the environment, so that the skin has a phenomenon of lipid peroxidation. With the improvement of living standard of people, the functional requirements on skin care products are higher and higher. The essence is a skin care product added with concentrated high-nutrition components, such as plant extracts, ceramide, squalane and the like, and has the effects of resisting aging, resisting wrinkles, preserving moisture, whitening, removing spots and the like according to the pertinence of the formula. The source of the functional components is mainly divided into chemical and plant types, wherein the natural plant type has the characteristics of naturalness, mildness, no toxic or side effect and the like, is well liked by consumers and has wide market prospect.

At present, application research for increasing stem cell active factors in skin care essence is carried out, but no report that plant cell extract is used for producing skin care essence is found.

Disclosure of Invention

The invention aims to provide a skin care essence containing a cell extracting solution, which has the effects of resisting aging, resisting wrinkles and moisturizing.

The invention also aims to provide a preparation method of the skin care essence containing the cell extracting solution, which is simple to prepare and can be used for industrial production.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a skin care essence containing cell extract comprises the following components in parts by weight: 10-15 parts of purple sweet potato cell extract, 2-4 parts of ginseng extract, 0.5-1 part of peony root extract, 0.25-0.5 part of mulberry leaf extract, 0.08-0.2 part of tremella polysaccharide, 0.4-0.6 part of nicotinamide, 0.2-0.4 part of tocopherol, 0.5-1.0 part of trehalose, 0.5-1.5 parts of betaine, 2-5 parts of glycerol, 3-5 parts of dipropylene glycol, 0.4-0.5 part of thickener, 0.25-0.4 part of triethanolamine, 0.08-0.12 part of chelating agent and 65-80 parts of water.

Preferably, the skin care essence containing the cell extracting solution comprises the following components in parts by weight: 12 parts of purple sweet potato cell extracting solution, 3 parts of ginseng extract, 0.8 part of peony root extract, 0.4 part of mulberry leaf extract, 0.15 part of tremella polysaccharide, 0.5 part of nicotinamide, 0.3 part of tocopherol, 0.8 part of trehalose, 1.15 parts of betaine, 4 parts of glycerol, 4 parts of dipropylene glycol, 0.45 part of thickening agent, 0.3 part of triethanolamine, 0.1 part of chelating agent and 78 parts of water.

Preferably, the thickening agent is one or more of carbomer and xanthan gum.

Preferably, the chelating agent is disodium EDTA.

Preferably, the water is pure water with the conductivity less than or equal to 2 mu S/cm.

Further, the skin care essence containing the cell extracting solution further comprises one or more of an aromatic, a toner and a preservative.

The invention also provides a preparation method of the skin care essence containing the cell extracting solution, which comprises the following steps:

(1) centrifuging the purple sweet potato cell culture solution, removing the precipitate, freezing and crushing the supernatant at ultralow temperature by using liquid nitrogen to obtain purple sweet potato cell powder;

(2) taking a proper amount of purple sweet potato cell powder, adding 50mmol/L phosphate buffer solution with pH of 7.0 to obtain purple sweet potato cell sap with the concentration of 20mg/mL, repeatedly freezing and thawing for five times at-80 ℃, centrifuging at 3-5 ℃, taking supernate, namely purple sweet potato cell extracting solution, and storing at 3-5 ℃ for later use;

(3) adding accurately weighed water, glycerol, betaine, trehalose, dipropylene glycol, thickening agent and chelating agent into an emulsifying pot, stirring, heating to 85-90 deg.C, stirring, and sterilizing at constant temperature for 20-25 min;

(4) starting circulating cooling water, continuing stirring, cooling the temperature in the emulsifying pot to 42-48 deg.C, adding accurately weighed purple sweet potato cell extractive solution, Ginseng radix extract, peony root extract, folium Mori extract, Tremella polysaccharide, nicotinamide, tocopherol and triethanolamine, and stirring;

(5) and starting circulating cooling water, continuing stirring until the temperature of the material is reduced to 38 ℃, stopping stirring after the pH value is qualified, and discharging.

Preferably, the extraction method of the purple sweet potato cell culture solution is as follows: taking fresh and non-rotten purple sweet potatoes as a raw material, cleaning, cutting into blocks, cooking, pulping with water according to the weight ratio of 1: 1.5-2.5, and then carrying out enzymolysis by adopting alpha-amylase and glucoamylase at the temperature of 50-70 ℃ for 60-120min, wherein the addition amount of the alpha-amylase is 0.01-0.09% of the weight of the purple sweet potato pulp raw material, and the addition amount of the glucoamylase is 0.01-0.05% of the weight of the purple sweet potato pulp raw material, so as to prepare a purple sweet potato saccharification liquid; inoculating 6-12% yeast seed culture solution at volume ratio, culturing at 25-30 deg.C for 3-4 days with pH of 3.0-3.5, and filtering after fermentation.

Preferably, in step (1), the centrifugation conditions are: the rotating speed is 3000-.

Preferably, in the step (2), the centrifugation conditions are as follows: the rotating speed is 8000-.

The pharmacological mechanism analysis of each active component in the formula of the invention is as follows:

purple sweet potato cell extracting solution: rich in natural anthocyanins, has hydroxyl radical scavenging activity, and can inhibit elastase, protect collagen protein and improve skin elasticity;

the ginseng extract: delaying skin aging, preventing skin dryness and dehydration, increasing skin elasticity, protecting skin luster, tendering, preventing and reducing skin wrinkle, and inhibiting melanin reduction to make skin white and smooth.

Peony root extract: the skin care product is rich in amino acids, mineral substances, various vitamins, zinc, selenium and other trace elements required by a human body, has a strong relieving effect, and can reduce the activity of melanin in skin keratinocytes so as to help inhibit the generation of melanin; has antibacterial and antiinflammatory effects; the cream stimulates the lymphatic circulation of the skin, makes the skin glossy, improves the skin quality, and can make the skin slowly volatilize dull and rough, and make the skin bright and moist after being continuously used.

And (3) mulberry leaf extract: contains various physiological active substances such as folium Mori flavone, folium Mori polyphenol, folium Mori polysaccharide, etc., and has effects of inhibiting elastase, delaying aging, inhibiting tyrosinase activity, and whitening skin.

White fungus polysaccharide: the skin care product can activate epidermal cells, repair photodamaged skin, accelerate skin regeneration, and form a uniform film with a certain thickness on the surface of the skin so that the skin has good smooth and moist feeling;

nicotinamide: the skin-care product can effectively reduce the generation of melanin, accelerate the metabolism of melanin horny cells, promote the synthesis of skin epidermal protein and greatly improve the defense force of skin;

tocopherol is a hydrolysate of vitamin E, is one of the most main antioxidants, has fat solubility, can protect skin, enable the skin to have elasticity, stabilize the protein active structure of a cell membrane, promote the normal development of muscle, keep the elasticity of the skin and enable the skin and the body to keep vitality; the skin cell entering the skin cell can directly help the skin to resist the invasion of free radicals, ultraviolet rays and pollutants, and prevent the skin from losing elasticity until aging due to some chronic or recessive injuries.

Trehalose, betaine, glycerol and dipropylene glycol are used as moisturizers, and can improve the moisture content and the moisture retention of the skin.

Thickening agent: has effects in thickening and improving skin feeling.

Triethanolamine: and the pH regulator is used for regulating the pH value of the system.

Chelating agent: chelating the metal ions.

Compared with the prior art, the invention has the following beneficial effects:

1. the essence disclosed by the invention is added with the purple sweet potato cell extracting solution, and the cells are crushed at ultralow temperature by adopting liquid nitrogen, so that the biological activity of substances in the cells is retained to the greatest extent, the high cell breakage rate is achieved, the anthocyanin is completely released, the permeability is strong, the renewal and growth of the cells of an organism are promoted, and the activity of skin cells is improved.

2. The invention takes the purple sweet potato cell extract, the ginseng extract, the peony root extract and the mulberry leaf extract as main materials, and is supplemented with the humectant, the thickener and the like, and the compatibility and the scientific proportion are reasonable, so that different effective active ingredients generate the synergistic interaction effect, and the product has excellent effects of removing hydroxyl free radicals, enhancing the skin elasticity, delaying the aging and supplementing the skin moisture.

3. The essence disclosed by the invention is mild and non-irritant to skin, good in safety, simple in preparation process and suitable for industrial production.

Detailed Description

The present invention will be described in further detail with reference to specific examples.