阅读说明：本技术 一种含有富勒烯的抗衰组合物及其制备方法和应用 (Anti-aging composition containing fullerene and preparation method and application thereof ) 是由 张洪莲 钱德玉 于 2021-07-15 设计创作，主要内容包括：本发明公开了一种含有富勒烯的抗衰组合物及其制备方法和应用,所述的含有富勒烯的抗衰组合物,按重量份计,包括：0.4～1份水溶性富勒烯、0.5～1.2份岩藻糖、0.6～1.5份红景天根提取物、0.8～1.5份玄参提取物、1～1.8份玉米须提取物、10～20份保湿剂、75～90份去离子水。所述的组合物具有良好的清除自由基作用,能够有效的解决肌肤因自由基带来的肌肤暗黄衰老松弛,使肌肤充满弹性,机体组织得到恢复与强化,恢复肌肤组织弹力,修护受损肌肤,促进肌肤防护屏障功能；本发明通过加入水溶性富勒烯、玄参提取物、玉米须提取物显著提高清除自由基效果,从而达到良好的抗衰作用。(The invention discloses an anti-aging composition containing fullerene, a preparation method and an application thereof, wherein the anti-aging composition containing fullerene comprises the following components in parts by weight: 0.4-1 part of water-soluble fullerene, 0.5-1.2 parts of fucose, 0.6-1.5 parts of rhodiola root extract, 0.8-1.5 parts of radix scrophulariae extract, 1-1.8 parts of corn stigma extract, 10-20 parts of humectant and 75-90 parts of deionized water. The composition has a good effect of removing free radicals, can effectively solve the problem that skin is dark yellow, aged and relaxed due to the free radicals, enables the skin to be full of elasticity, recovers and strengthens body tissues, recovers the elasticity of the skin tissues, repairs the damaged skin and promotes the skin protection barrier function; the effect of removing free radicals is obviously improved by adding the water-soluble fullerene, the figwort root extract and the corn stigma extract, so that a good anti-aging effect is achieved.)

1. An anti-aging composition containing fullerene is characterized by comprising the following components in parts by weight: 0.4-1 part of water-soluble fullerene, 0.5-1.2 parts of fucose, 0.6-1.5 parts of rhodiola root extract, 0.8-1.5 parts of radix scrophulariae extract, 1-1.8 parts of corn stigma extract, 10-20 parts of humectant and 75-90 parts of deionized water.

2. The anti-aging composition containing fullerene according to claim 1, comprising, in parts by weight: 0.5-1 part of water-soluble fullerene, 0.5-1 part of fucose, 0.8-1.5 parts of rhodiola root extract, 1-1.5 parts of figwort root extract, 1.2-1.8 parts of corn stigma extract, 12-20 parts of humectant and 75-85 parts of deionized water.

3. The anti-aging composition containing fullerene according to claim 1, comprising, in parts by weight: 0.7 part of water-soluble fullerene, 0.8 part of fucose, 1 part of rhodiola root extract, 1.2 parts of figwort root extract, 1.5 parts of corn stigma extract, 15 parts of humectant and 79.8 parts of deionized water.

4. The anti-aging composition containing fullerene as claimed in claim 1, wherein the humectant is one or more of glycerin, 1, 3-butanediol, 1, 3-propanediol, 1, 2-pentanediol, sorbitol, saccharide isomer, panthenol.

5. The anti-aging composition containing fullerene as claimed in claim 1, wherein the humectant is prepared from glycerin, 1, 3-butylene glycol in a weight ratio of 1: 0.5 to 2.

6. The anti-aging composition containing fullerene as claimed in claim 1, wherein the figwort extract is prepared by a method comprising:

s01, taking the figwort root as a medicinal material, cleaning, drying and grinding to 50-100 meshes to obtain figwort root powder;

s02, placing the radix scrophulariae powder in a distillation retort, introducing steam for distillation for 1-4 hours to obtain an oil-water mixture, and collecting a water phase;

and S03, feeding the water phase into a D101 type macroporous adsorption resin column at the rate of 0.5-1.2 BV/h, eluting at the rate of 0.5-1.2 BV/h, washing with deionized water for 0.8-1.5 BV, washing with 50-80 wt% ethanol solution for 1-1.8 BV, collecting ethanol solution eluent, and drying to obtain the radix scrophulariae extract.

7. The anti-aging composition containing fullerene according to claim 1, wherein the corn silk extract is prepared by the following steps:

s1, cleaning, drying and grinding the corn silk to 40-80 meshes to obtain corn silk powder;

s2, adding the corn silk powder into deionized water, and extracting at 50-60 ℃ for 30-60 min to obtain a mixed solution, wherein the ratio of the corn silk powder to the deionized water is 1 g: 6-10 ml;

s3, adding the mixed enzyme into the mixed solution, keeping the mixed solution at 50-60 ℃ for 50-70 min, centrifuging, and taking supernatant, wherein the weight ratio of the mixed enzyme to the mixed solution is (0.02-0.05): 1;

s4, putting the supernatant into a freezing box, quickly reducing the temperature of the freezing box to-35 to-30 ℃, starting a vacuum pump, vacuumizing the freezing box to 35-45 Pa, keeping the temperature and the vacuum degree for 50-60 min, adjusting the temperature of the freezing box to-25 to-15 ℃, keeping the vacuum degree for 28-32 Pa, keeping the temperature for 45-50 min, increasing the temperature of the freezing box to-5 ℃, adjusting the vacuum degree to 20-30 Pa, keeping the temperature for 40-50 min, adjusting the temperature of the freezing box to 10-20 ℃, adjusting the vacuum degree to 15-25 Pa, keeping the temperature for 60-100 min, and finally, stopping freeze-drying when the vacuum degree is restored to the atmospheric pressure to obtain the corn silk extract.

8. The anti-aging composition containing fullerene as claimed in claim 7, wherein the mixed enzyme is prepared from pectinase, cellulase and protease at a weight ratio of 1: 0.5-2: 0.4 to 1.

9. The method for preparing an antiaging composition containing fullerene as claimed in any one of claims 1 to 8, characterized by comprising the steps of:

adding humectant into ionized water, mixing, adding water-soluble fullerene, fucose, radix Rhodiolae extract, radix scrophulariae extract, and stigma Maydis extract, and stirring to obtain antiaging composition containing fullerene.

10. Use of the anti-aging composition prepared according to claim 9 for the preparation of anti-aging cosmetics.

Technical Field

The invention relates to the technical field of cosmetics, in particular to an anti-aging composition containing fullerene and a preparation method and application thereof.

Background

Human body aging is a biological change process, and is caused by interaction of various factors such as genetic genes and complex internal and external environments, and as the body ages, unsaturated fatty acids in cell membranes are damaged by generated free radicals when the free radicals are excessive or antioxidants are deficient, so that lipid peroxidation is caused, and the structure of biological membranes is greatly damaged. In the aerobic metabolism process of aging, the oxidation resistance of an organism is reduced due to the continuous increase of the level of free radicals, the increase of the free radicals can lead the skin to be dark yellow, aged and relaxed, the cytotoxicity is increased to different degrees to cause the instant irreversible change and damage of the structure, the stimulation of the environment and the outside, mitochondria and fatty acid can generate various peroxidation factors such as superoxide anion, hydrogen peroxide, hydroxyl free radical and the like, a physiological signal path can be triggered, the peroxidation and the apoptosis in cells are induced, and the aging process is accelerated and promoted. Finally, oxidative aging and apoptosis together cause the body to develop a senescence phenomenon.

In the field of cosmetics, fullerene has excellent values of anti-wrinkle, whitening and aging prevention because it has four major effects of anti-free radical, anti-oxidation, spot-lightening and whitening, and inflammation inhibition. Since 21 century, it has become a very attractive cosmetic ingredient and even known as "the King of anti-aging". The fullerene has wide application prospect in anti-aging cosmetics.

Disclosure of Invention

The invention provides an anti-aging composition containing fullerene, and a preparation method and application thereof.

The invention adopts the following technical scheme for solving the technical problems:

an anti-aging composition containing fullerene comprises the following components in parts by weight: 0.4-1 part of water-soluble fullerene, 0.5-1.2 parts of fucose, 0.6-1.5 parts of rhodiola root extract, 0.8-1.5 parts of radix scrophulariae extract, 1-1.8 parts of corn stigma extract, 10-20 parts of humectant and 75-90 parts of deionized water.

The inventor of the invention finds that the anti-aging composition has better anti-aging effect under the weight part ratio.

As a preferable scheme, the paint comprises the following components in parts by weight: 0.5-1 part of water-soluble fullerene, 0.5-1 part of fucose, 0.8-1.5 parts of rhodiola root extract, 1-1.5 parts of figwort root extract, 1.2-1.8 parts of corn stigma extract, 12-20 parts of humectant and 75-85 parts of deionized water.

As a preferable scheme, the paint comprises the following components in parts by weight: 0.7 part of water-soluble fullerene, 0.8 part of fucose, 1 part of rhodiola root extract, 1.2 parts of figwort root extract, 1.5 parts of corn stigma extract, 15 parts of humectant and 79.8 parts of deionized water.

Preferably, the humectant is one or more of glycerin, 1, 3-butanediol, 1, 3-propanediol, 1, 2-pentanediol, sorbitol, saccharide isomer, and panthenol.

As a preferable scheme, the humectant is prepared from glycerol and 1, 3-butanediol in a weight ratio of 1: 0.5 to 2.

As a preferred scheme, the preparation method of the figwort root extract comprises the following steps:

s01, taking the figwort root as a medicinal material, cleaning, drying and grinding to 50-100 meshes to obtain figwort root powder;

s02, placing the radix scrophulariae powder in a distillation retort, introducing steam for distillation for 1-4 hours to obtain an oil-water mixture, and collecting a water phase;

and S03, feeding the water phase into a D101 type macroporous adsorption resin column at the rate of 0.5-1.2 BV/h, eluting at the rate of 0.5-1.2 BV/h, washing with deionized water for 0.8-1.5 BV, washing with 50-80 wt% ethanol solution for 1-1.8 BV, collecting ethanol solution eluent, and drying to obtain the radix scrophulariae extract.

The inventor of the invention finds that through steam distillation and macroporous adsorption resin adsorption treatment on the radix scrophulariae medicinal material, components with the effect of scavenging free radicals are effectively reserved, and the radix scrophulariae extract with good effect of scavenging free radicals is obtained, and the inventor finds that the composition prepared from the radix scrophulariae extract prepared by the preparation method has better effect of scavenging free radicals than the radix scrophulariae extract obtained by other methods.

As a preferred scheme, the preparation method of the corn stigma extract comprises the following steps:

s1, cleaning, drying and grinding the corn silk to 40-80 meshes to obtain corn silk powder;

s2, adding the corn silk powder into deionized water, and extracting at 50-60 ℃ for 30-60 min to obtain a mixed solution, wherein the ratio of the corn silk powder to the deionized water is 1 g: 6-10 ml;

s3, adding the mixed enzyme into the mixed solution, keeping the mixed solution at 50-60 ℃ for 50-70 min, centrifuging, and taking supernatant, wherein the weight ratio of the mixed enzyme to the mixed solution is (0.02-0.05): 1;

s4, putting the supernatant into a freezing box, quickly reducing the temperature of the freezing box to-35 to-30 ℃, starting a vacuum pump, vacuumizing the freezing box to 35-45 Pa, keeping the temperature and the vacuum degree for 50-60 min, adjusting the temperature of the freezing box to-25 to-15 ℃, keeping the vacuum degree for 28-32 Pa, keeping the temperature for 45-50 min, increasing the temperature of the freezing box to-5 ℃, adjusting the vacuum degree to 20-30 Pa, keeping the temperature for 40-50 min, adjusting the temperature of the freezing box to 10-20 ℃, adjusting the vacuum degree to 15-25 Pa, keeping the temperature for 60-100 min, and finally, stopping freeze-drying when the vacuum degree is restored to the atmospheric pressure to obtain the corn silk extract.

The inventors of the present invention have found that a corn silk extract having excellent free radical scavenging activity is obtained by subjecting corn silk to water treatment, enzyme treatment, and freeze-drying, wherein the free radical scavenging activity is obtained by mixing a raw material consisting of pectinase, cellulase, and protease in a weight ratio of 1: 0.5-2: the mixed enzyme consisting of 0.4-1 can fully crush the corn stigma cells, can better extract components capable of removing free radicals, and can effectively preserve the components capable of removing the free radicals by controlling related process parameters of freeze drying, so that the corn stigma extract capable of removing the free radicals is obtained.

Preferably, the mixed enzyme is prepared from pectinase, cellulase and protease according to a weight ratio of 1: 0.5-2: 0.4 to 1.

The invention also provides a preparation method of the anti-aging composition containing fullerene, which comprises the following steps:

adding humectant into ionized water, mixing, adding water-soluble fullerene, fucose, radix Rhodiolae extract, radix scrophulariae extract, and stigma Maydis extract, and stirring to obtain antiaging composition containing fullerene.

The water-soluble fullerene is water-soluble fullerene C60, and is purchased from Xiamen New Material science and technology Co.

The invention also provides application of the anti-aging composition containing fullerene in preparing anti-aging cosmetics.

According to the invention, the water-soluble fullerene, the figwort root extract and the corn stigma extract are matched with each other, so that the problem that the mechanism for removing free radicals and resisting aging is single is avoided, and the free radicals are removed from multiple angles.

The invention has the beneficial effects that: the composition has a good effect of removing free radicals, can effectively solve the problem that skin is dark yellow, aged and relaxed due to the free radicals, enables the skin to be full of elasticity, recovers and strengthens body tissues, recovers the elasticity of the skin tissues, repairs the damaged skin and promotes the skin protection barrier function; the effect of removing free radicals is obviously improved by adding the water-soluble fullerene, the figwort root extract and the corn stigma extract, so that a good anti-aging effect is achieved.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the present invention, the parts are all parts by weight unless otherwise specified.

Example 1

An anti-aging composition containing fullerene comprises the following components in parts by weight: 0.7 part of water-soluble fullerene, 0.8 part of fucose, 1 part of rhodiola root extract, 1.2 parts of figwort root extract, 1.5 parts of corn stigma extract, 15 parts of humectant and 79.8 parts of deionized water.

The humectant is prepared from glycerol and 1, 3-butanediol in a weight ratio of 1: 0.5.

The preparation method of the figwort root extract comprises the following steps:

s01, taking the figwort root as a medicinal material, cleaning, drying and grinding to 80 meshes to obtain figwort root powder;

s02, placing the radix scrophulariae powder in a distillation retort, introducing steam for distillation for 2 hours to obtain an oil-water mixture, and collecting a water phase;

and S03, feeding the water phase into a D101 type macroporous adsorption resin column at the rate of 1BV/h, washing the water phase with deionized water for 1BV, then washing with 70 wt% ethanol solution for 1.5BV, collecting ethanol solution eluent, and drying to obtain the radix scrophulariae extract.

The preparation method of the corn stigma extract comprises the following steps:

s1, cleaning the corn silk, drying and grinding the corn silk to 50 meshes to obtain corn silk powder;

s2, adding the corn silk powder into deionized water, and extracting at 58 ℃ for 40min to obtain a mixed solution, wherein the ratio of the corn silk powder to the deionized water is 1 g: 9 ml;

s3, adding the mixed enzyme into the mixed solution, keeping the mixed solution at 55 ℃ for 60min, centrifuging the mixed solution at 5000rpm for 10min, and taking supernatant, wherein the weight ratio of the mixed enzyme to the mixed solution is 0.04: 1;

s4, putting the supernatant into a freezing box, quickly reducing the temperature of the freezing box to-32 ℃, starting a vacuum pump, vacuumizing the freezing box to 40Pa, keeping the temperature and the vacuum degree for 55min, adjusting the temperature of the freezing box to-20 ℃, keeping the vacuum degree for 30 Pa for 48min, increasing the temperature of the freezing box to-0 ℃, adjusting the vacuum degree for 25Pa, and keeping the vacuum degree for 45 min; and adjusting the temperature of the freeze-drying box to 15 ℃, adjusting the vacuum degree to 20Pa, keeping for 80min, and finally, stopping freeze-drying when the vacuum degree is restored to the atmospheric pressure to obtain the corn stigma extract.

The mixed enzyme is prepared from pectinase, cellulase and protease according to the weight ratio of 1: 1: 0.5.

The preparation method of the anti-aging composition containing fullerene comprises the following steps:

adding humectant into ionized water, mixing, adding water-soluble fullerene, fucose, radix Rhodiolae extract, radix scrophulariae extract, and stigma Maydis extract, and stirring to obtain antiaging composition containing fullerene.

Example 2

An anti-aging composition containing fullerene comprises the following components in parts by weight: 0.4 part of water-soluble fullerene, 1.2 parts of fucose, 1.2 parts of rhodiola rosea root extract, 1 part of figwort root extract, 1.4 parts of corn stigma extract, 16 parts of humectant and 77.6 parts of deionized water.

The humectant is prepared from glycerol and 1, 3-butanediol in a weight ratio of 1: 1, the product is obtained.

The preparation method of the figwort root extract comprises the following steps:

s01, taking the figwort root as a medicinal material, cleaning, drying and grinding to 90 meshes to obtain figwort root powder;

s02, placing the radix scrophulariae powder in a distillation retort, introducing steam for distillation for 2 hours to obtain an oil-water mixture, and collecting a water phase;

and S03, feeding the water phase into a D101 type macroporous adsorption resin column at the rate of 1BV/h, washing the water phase with deionized water for 1BV, then washing with 70 wt% ethanol solution for 1.5BV, collecting ethanol solution eluent, and drying to obtain the radix scrophulariae extract.

The preparation method of the corn stigma extract comprises the following steps:

s1, cleaning the corn silk, drying and grinding the corn silk to 50 meshes to obtain corn silk powder;

s2, adding the corn silk powder into deionized water, and extracting at 52 ℃ for 50min to obtain a mixed solution, wherein the ratio of the corn silk powder to the deionized water is 1 g: 6 ml;

s3, adding the mixed enzyme into the mixed solution, keeping the mixed solution at 55 ℃ for 60min, centrifuging, and taking supernatant, wherein the weight ratio of the mixed enzyme to the mixed solution is 0.03: 1;

s4, putting the supernatant into a freezing box, quickly reducing the temperature of the freezing box to-32 ℃, starting a vacuum pump, vacuumizing the freezing box to 40Pa, keeping the temperature and the vacuum degree for 55min, adjusting the temperature of the freezing box to-20 ℃, keeping the vacuum degree for 30 Pa and 48min, increasing the temperature of the freezing box to-0 ℃, adjusting the vacuum degree to 25Pa, keeping the vacuum degree for 45min, adjusting the temperature of the freezing box to 15 ℃, adjusting the vacuum degree to 20Pa, keeping the vacuum degree for 80min, and finally, stopping freeze-drying when the vacuum degree is restored to the atmospheric pressure to obtain the corn silk extract.

The mixed enzyme is prepared from pectinase, cellulase and protease according to the weight ratio of 1: 0.8: 0.7.

The preparation method of the anti-aging composition containing fullerene comprises the following steps:

adding humectant into ionized water, mixing, adding water-soluble fullerene, fucose, radix Rhodiolae extract, radix scrophulariae extract, and stigma Maydis extract, and stirring to obtain antiaging composition containing fullerene.

Example 3

An anti-aging composition containing fullerene comprises the following components in parts by weight: 0.6 part of water-soluble fullerene, 1.2 parts of fucose, 0.6 part of rhodiola root extract, 1.2 parts of figwort root extract, 1 part of corn stigma extract, 18 parts of humectant and 77.4 parts of deionized water.

The humectant is prepared from glycerol and 1, 3-butanediol in a weight ratio of 1: 1.

The preparation method of the figwort root extract comprises the following steps:

s01, taking radix scrophulariae, cleaning, drying, and grinding to 60 meshes to obtain radix scrophulariae powder;

s02, placing the radix scrophulariae powder in a distillation retort, introducing steam for distillation for 2 hours to obtain an oil-water mixture, and collecting a water phase;

and S03, feeding the water phase into a D101 type macroporous adsorption resin column at the rate of 1BV/h, washing the water phase with deionized water for 1BV, then washing with 70 wt% ethanol solution for 1.5BV, collecting ethanol solution eluent, and drying to obtain the radix scrophulariae extract.

The preparation method of the corn stigma extract comprises the following steps:

s1, cleaning the corn silk, drying and grinding the corn silk to 70 meshes to obtain corn silk powder;

s2, adding the corn silk powder into deionized water, and extracting at 50 ℃ for 60min to obtain a mixed solution, wherein the ratio of the corn silk powder to the deionized water is 1 g: 10 ml;

s3, adding the mixed enzyme into the mixed solution, keeping the mixed solution at 55 ℃ for 60min, centrifuging the mixed solution at 5000rpm for 10min, and taking supernatant, wherein the weight ratio of the mixed enzyme to the mixed solution is 0.04: 1;

s4, putting the supernatant into a freezing box, quickly reducing the temperature of the freezing box to-30 ℃, starting a vacuum pump, vacuumizing the freezing box to 40Pa, keeping the temperature and the vacuum degree for 55min, adjusting the temperature of the freezing box to-20 ℃, and keeping the vacuum degree for 30 Pa for 48 min; the temperature of the freeze-drying box is raised to-0 ℃, the vacuum degree is adjusted to 25Pa, and the freeze-drying box is kept for 45 min; and adjusting the temperature of the freeze-drying box to 15 ℃, adjusting the vacuum degree to 20Pa, keeping for 80min, and finally, stopping freeze-drying when the vacuum degree is restored to the atmospheric pressure to obtain the corn stigma extract.

The mixed enzyme is prepared from pectinase, cellulase and protease according to the weight ratio of 1: 1: 0.5.

The preparation method of the anti-aging composition containing fullerene comprises the following steps:

adding humectant into ionized water, mixing, adding water-soluble fullerene, fucose, radix Rhodiolae extract, radix scrophulariae extract, and stigma Maydis extract, and stirring to obtain antiaging composition containing fullerene.

Comparative example 1

Comparative example 1 is different from example 1 in that comparative example 1 does not contain the figwort extract, and the others are the same.

Comparative example 2

Comparative example 2 is different from example 1 in that the figwort root extract is prepared by the same method as example 1, but otherwise.

The preparation method of the figwort root extract comprises the following steps:

s01, taking the figwort root as a medicinal material, cleaning, drying and grinding to 80 meshes to obtain figwort root powder;

s02, placing the radix scrophulariae powder in a distillation retort, introducing steam for distillation for 2h to obtain an oil-water mixture, collecting a water phase, and drying to obtain the radix scrophulariae extract.

Comparative example 3

Comparative example 3 is different from example 1 in that the figwort root extract is obtained by water extraction, and the rest is the same.

The preparation method of the figwort root extract comprises the following steps:

s01, taking the figwort root as a medicinal material, cleaning, drying and grinding to 80 meshes to obtain figwort root powder;

s02, placing the radix scrophulariae powder in a triangular flask, adding deionized water, leaching at 60 ℃ for 4h, centrifuging at 5000rpm for 10min, filtering, and drying to obtain radix scrophulariae extract.

The feed-liquid ratio of the radix scrophulariae powder to the deionized water is 1 g: 6 ml.

Comparative example 4

Comparative example 4 differs from example 1 in that comparative example 4 does not contain the stigma Maydis extract, and the other things are the same.

Comparative example 5

Comparative example 5 is different from example 1 in that the corn silk extract of comparative example 5 is prepared by a method different from example 1, and is otherwise the same, and an enzymatic hydrolysis step is not used in this comparative example.

The preparation method of the corn stigma extract comprises the following steps:

s1, cleaning the corn silk, drying and grinding the corn silk to 50 meshes to obtain corn silk powder;

s2, adding the corn silk powder into deionized water, extracting for 40min at 58 ℃ to obtain a mixed solution, centrifuging for 10min at the rotating speed of 5000rpm, and taking supernatant, wherein the ratio of the corn silk powder to the deionized water is 1 g: 9 ml;

s3, putting the supernatant into a freezing box, quickly reducing the temperature of the freezing box to-32 ℃, starting a vacuum pump, vacuumizing the freezing box to 40Pa, keeping the temperature and the vacuum degree for 55min, adjusting the temperature of the freezing box to-20 ℃, keeping the vacuum degree for 30 Pa for 48min, increasing the temperature of the freezing box to-0 ℃, adjusting the vacuum degree for 25Pa, and keeping the vacuum degree for 45 min; and adjusting the temperature of the freeze-drying box to 15 ℃, adjusting the vacuum degree to 20Pa, keeping for 80min, and finally, stopping freeze-drying when the vacuum degree is restored to the atmospheric pressure to obtain the corn stigma extract.

Comparative example 6

Comparative example 6 is different from example 1 in that comparative example 6 does not contain the water-soluble fullerene, and the others are the same.

To further demonstrate the effect of the present invention, the following test methods were provided:

determination of DPPH scavenging ability:

the mixture of DPPH powder (7.8 mg) and absolute ethanol (100 mL) was weighed to obtain a 0.2mmol/L mixed solution of DPPH and absolute ethanol.

Accurately sucking 200 mu L of DPPH absolute ethyl alcohol mixed solution and 200 mu L of sample solution to be tested (the anti-aging composition described in examples 1-3 and comparative examples 1-6), shaking, uniformly mixing, and standing at room temperature in a dark place for 30 min. Accurately sucking 200 mul of absolute ethyl alcohol solution and 200 mul of sample solution to be tested, shaking and mixing uniformly. Mixing 200 μ L of distilled water and 200 μ L of DPPH anhydrous ethanol, shaking and mixing. Measuring A in enzyme-linked immunosorbent assay_517nm。

Clearance (%) - (1- (A)_a-A_b)/A_c]× 100％

In the formula: aa is the absorbance value of 200 mu L of each of the sample solution and the DPPH absolute ethyl alcohol mixed solution; a. the_bAbsorbance values of 200. mu.L each of the absolute ethanol and the sample solution; a. the_cThe absorbance values of 200. mu.L each of the DPPH absolute ethanol mixture and distilled water are shown in Table 1.

2. Determination of hydroxyl radical scavenging (. OH)

Performing operation according to the instruction of hydroxyl free radical determination kit (Nanjing Kaihuahao Biotechnology limited, Cat. A018-1-1), and measuring A with enzyme labeling instrument_550nm. Distilled water was used as a blank control. Vitamin C is used as a positive control, and the test group is the anti-aging composition described in examples 1-3 and comparative examples 1-6.

Clearance (%) ═ a₁-A₂)/(A₁-A₃) X 100% of formula: a. the₁OD value for control tube, A₂To test the OD of the tubes, A₃OD value of blank tube.

TABLE 1

As can be seen from Table 1, the antiaging composition of the invention has good effect of scavenging free radicals.

In comparative examples 1 to 3, it can be seen that the ratio of raw materials of different anti-aging compositions, the preparation parameters of the radix scrophulariae extract and the preparation parameters of the stigma Maydis extract can affect the clearance rate of free radicals.

Compared with the comparative examples 1 to 3, the radix scrophulariae extract disclosed by the invention can obviously improve the clearance rate of free radicals, different preparation methods of the radix scrophulariae extract can influence the fresh fruit of the radix scrophulariae extract for clearing the free radicals, and the radix scrophulariae extract prepared by the preparation method of the radix scrophulariae extract disclosed by the invention has a better effect of clearing the free radicals compared with the radix scrophulariae extracts obtained by other methods.

Comparing example 1 with comparative examples 4 and 5, it can be seen that the corn stigma extract of the present invention can significantly improve the clearance rate of free radicals.

Comparing example 1 with comparative example 6, it is understood that the addition of fullerene can improve the clearance rate for the radical.

In light of the foregoing description of preferred embodiments according to the invention, it is clear that many changes and modifications can be made by the person skilled in the art without departing from the scope of the invention. The technical scope of the present invention is not limited to the contents of the specification, and must be determined according to the scope of the claims.