阅读说明：本技术 一种svf-gel脂肪胶制备方法 (Preparation method of SVF-GEL fat gum ) 是由 王立惠 于 2020-07-08 设计创作，主要内容包括：本发明公开了一种SVF-GEL脂肪胶制备方法,具体步骤如下：第一步：将抽吸所获得的脂肪,进行机械乳化,静置5分钟；第二步：将提取出的脂肪组织放置在高速离心机中进行第一次离心提纯,离心机控制在1000-1800/min,离心得到富含脂肪干细胞和细胞外基质的产物；第三步：将步骤二中第一次离心提纯后得到液体、油滴、获取乳糜状的纳米脂肪单独分离出油滴静置5小时备用；第四步：将步骤二中第一离心提纯后的纳米脂肪与步骤三中的油滴按照比例混合,通过磁力搅拌使其变成絮状物。本发明可以促使皮肤胶原蛋白的合成,达到改善肤质,抗皱促进皮肤再生的效果,完全可以填充更为精细的皱纹,这是传统科尔曼脂肪做不到的。(The invention discloses a preparation method of SVF-GEL fat glue, which comprises the following specific steps: the first step is as follows: mechanically emulsifying the fat obtained by suction, and standing for 5 minutes; the second step is that: placing the extracted adipose tissues in a high-speed centrifuge for first centrifugal purification, controlling the centrifuge at 1000-; the third step: performing centrifugal purification for the first time in the second step to obtain liquid and oil drops, and obtaining chyliform nano fat to separate the oil drops independently and standing for 5 hours for later use; the fourth step: and (3) mixing the nano fat after the first centrifugal purification in the step two with the oil drops in the step three according to a ratio, and stirring the mixture into floccules by magnetic force. The invention can promote the synthesis of skin collagen, achieve the effects of improving skin quality, resisting wrinkles and promoting skin regeneration, and completely fill finer wrinkles, which cannot be done by the traditional Coleman fat.)

1. A preparation method of SVF-GEL fat glue is characterized by comprising the following specific steps:

the first step is as follows: mechanically emulsifying the fat obtained by suction, and standing for 5 minutes;

the second step is that: placing the extracted adipose tissues in a high-speed centrifuge for first centrifugal purification, controlling the centrifuge at 1000-;

the third step: performing centrifugal purification for the first time in the second step to obtain liquid and oil drops, and obtaining chyliform nano fat to separate the oil drops independently and standing for 5 hours for later use;

the fourth step: mixing the nano fat after the first centrifugal purification in the step two with the oil drops in the step three according to a proportion, and stirring the mixture into floccules by magnetic force;

the fifth step: standing the floccule in the fourth step for 5 hours at the temperature of 0-5 ℃, and then carrying out secondary purification;

and a sixth step: in the step five, the secondary purification is controlled at 1000-1800/min through a high-speed centrifuge,

the chyliform nano fat with higher concentration is divided into oil drops, fat and liquid again, and the liquid at the bottommost layer and the oil drops at the topmost layer are removed;

the seventh step: the nano fat obtained by centrifugation in the sixth step contains high-concentration fat gel of fat stem cells, so that the synthesis of skin collagen can be promoted, and the effects of improving skin quality, resisting wrinkles and promoting skin regeneration are achieved;

eighth step: and (5) filling the nano fat obtained in the step seven into an ampere bottle, sealing and refrigerating for later use.

2. The method of claim 1, wherein the SVF-GEL fat gum is prepared by the following steps: the first centrifugation is controlled at 1500r/2-3min, and the centrifugation is carried out for 4 min.

3. The method of claim 1, wherein the SVF-GEL fat gum is prepared by the following steps: the second centrifugation is controlled at 1500-1600r/2-3min and is carried out for 8 min.

4. The method of claim 1, wherein the SVF-GEL fat gum is prepared by the following steps: in the secondary purification, 1ml of essence was concentrated and extracted from each 10ml of adipose tissues.

5. The method of claim 1, wherein the SVF-GEL fat gum is prepared by the following steps: the main components of the extracellular matrix product in the second step are a composition of collagen, elastin and mucopolysaccharide.

6. The method of claim 1, wherein the SVF-GEL fat gum is prepared by the following steps: the mixing ratio of the oil drops to the nano fat in the fourth step is 1: 4.

Technical Field

The invention relates to the technical field of beauty products, and particularly relates to a preparation method of SVF-GEL fat gum.

Background

Fat transplantation after negative pressure suction of fat from an extractable site and preliminary centrifugation-purification-standing, fat is divided into three layers, the middle layer being granular fat, i.e., coleman fat (coleman fat), which is the conventional fat filling currently performed for fat harvesting and filling transplantation, in other words, primarily with coleman fat.

When fat (Coleman fat) is filled and transplanted, fat tissues are in a free state, blood supply is lost (namely, no blood supplies nutrition for living and breeding to the fat tissues), necrosis or liquefaction can occur, the fat tissues are finally absorbed by a human body, the survival rate is not satisfactory, and nodules can occur to influence the appearance or the hand feeling of the effect.

Fat extraction, the current method applies a swelling liposuction method, and swelling liquid is prepared in each 1000mlNormal saline + 2% lidocaine 30ml + NAHCO₃40ml + 1ml epinephrine, and the fat in the deep part of the abdomen and thigh area is selected, the required amount of transplanted fat is firstly sucked by a syringe method and transferred into a centrifuge tube, and the sucking amount is generally twice as much as the injection amount.

And (3) preparing fat particles, namely standing the extract to remove blood and impurities, washing with physiological saline, filtering, and putting into a centrifuge, namely the zinc chandeli, wherein the speed is maintained at 600 rpm. Taking the middle and lower layers of adipose tissues in the tube for standby, extracting 50ml of venous blood of a customer, centrifuging, mixing with prepared fat particles, and transferring into a 1ml or 20ml syringe for standby.

The prior adipose stem cell GEL SVF-GEL prepared by the method of repeated injection and centrifugal deoiling in the fat transplantation is opposite to the structural adipose casing, and aims to destroy the adipose tissue structure and obtain the residual substances.

Disclosure of Invention

The invention aims to provide a preparation method of SVF-GEL fat glue, which can promote the regeneration of blood vessels, improve blood circulation, ensure that fat stem cells are differentiated into mature fat cells, promote the synthesis of skin collagen, achieve the effects of improving skin quality, resisting wrinkles and promoting the regeneration of skin and completely fill finer wrinkles.

The purpose of the invention is realized by the following technical scheme: a preparation method of SVF-GEL fat glue comprises the following specific steps:

the first step is as follows: mechanically emulsifying the fat obtained by suction, and standing for 5 minutes;

the second step is that: placing the extracted adipose tissues in a high-speed centrifuge for first centrifugal purification, controlling the centrifuge at 1000-;

the third step: performing centrifugal purification for the first time in the second step to obtain liquid and oil drops, and obtaining chyliform nano fat to separate the oil drops independently and standing for 5 hours for later use;

the fourth step: mixing the nano fat after the first centrifugal purification in the step two with the oil drops in the step three according to a proportion, and stirring the mixture into floccules by magnetic force;

the fifth step: standing the floccule in the fourth step for 5 hours at the temperature of 0-5 ℃, and then carrying out secondary purification;

and a sixth step: in the step five, the secondary purification is controlled at 1000-1800/min through a high-speed centrifuge,

the chyliform nano fat with higher concentration is divided into oil drops, fat and liquid again, and the liquid at the bottommost layer and the oil drops at the topmost layer are removed;

the seventh step: the nano fat obtained by centrifugation in the sixth step contains high-concentration fat gel of fat stem cells, so that the synthesis of skin collagen can be promoted, and the effects of improving skin quality, resisting wrinkles and promoting skin regeneration are achieved;

eighth step: and (5) filling the nano fat obtained in the step seven into an ampere bottle, sealing and refrigerating for later use.

As a preferred technical scheme: the first centrifugation is controlled at 1500r/2-3min, and the centrifugation is carried out for 4 min.

As a preferred technical scheme: the second centrifugation is controlled at 1500-1600r/2-3min and is carried out for 8 min.

As a preferred technical scheme: in the secondary purification, 1ml of essence was concentrated and extracted from each 10ml of adipose tissues.

As a preferred technical scheme: the main components of the extracellular matrix product in the second step are a composition of collagen, elastin and mucopolysaccharide.

As a preferred technical scheme: the mixing ratio of the oil drops to the nano fat in the fourth step is 1: 4.

Compared with the prior art, the invention has the beneficial effects that: the invention ensures the uniformity of fat injection through minimally invasive accurate injection, reduces the probability of generating unevenness and improves the survival rate of transplanted fat cells. In addition, the original pyramid layer-by-layer multidimensional filling technology has no swelling recovery period, can be kept for a long time, can effectively improve the skin for a long time, and is a magic instrument for facial rejuvenation. The traditional autologous fat recovery needs about two to three weeks, while the recovery period of the SVF-GEL inverse aged youthful gelatin only needs 3 to 5 days. And SVF-GEL inverse-age facial make-up glue does not damage and take out fat, is from self, and does not generate rejection phenomenon, and 1ml of essence can be concentrated and extracted from each 10ml of adipose tissue by an original ecological physiological combination of extracellular matrix and adipose stem cells in the adipose tissue, so that the GEL appearance similar to hyaluronic acid (hyaluronic acid) is presented. The components contain more substances such as fat stem cells, collagen, elastin and the like which have great benefits for the skin, and the substances can be called natural long-acting hyaluronic acid, and have obvious skin refreshing effect, the physical concentrated essence of adipose tissues is obtained, the high-concentrated substances obtained by the special process treatment of five-layer screening secondary centrifugation technology are accurately injected into the dermis layer and the subcutaneous layer of the skin through a 27G fine needle, wherein extracellular matrix (ECM) can supplement the collagen components lost in the dermis, epidermal growth factors, endothelial cell growth factors, fibroblast growth factors and the like which can be secreted by the fat stem cells act on the fibroblasts of the dermis layer, the higher purification degree and the relative survival rate can be greatly increased! The survival rate is ultrahigh, the problem of fibrosis caused by fat necrosis is avoided, 1-time filling is effective for the whole life, and the second filling is distinguished.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by those skilled in the art without any creative work based on the embodiments of the present invention belong to the protection scope of the present invention.

A preparation method of SVF-GEL fat glue comprises the following specific steps:

the first step is as follows: mechanically emulsifying the fat obtained by suction, and standing for 5 minutes;

the second step is that: placing the extracted adipose tissues in a high-speed centrifuge for first centrifugal purification, controlling the centrifuge at 1000-;

the third step: performing centrifugal purification for the first time in the second step to obtain liquid and oil drops, and obtaining chyliform nano fat to separate the oil drops independently and standing for 5 hours for later use;

the fourth step: mixing the nano fat after the first centrifugal purification in the step two with the oil drops in the step three according to a proportion, and stirring the mixture into floccules by magnetic force;

the fifth step: standing the floccule in the fourth step for 5 hours at the temperature of 0-5 ℃, and then carrying out secondary purification;

and a sixth step: in the step five, the secondary purification is controlled at 1000-1800/min through a high-speed centrifuge,

the chyliform nano fat with higher concentration is divided into oil drops, fat and liquid again, and the liquid at the bottommost layer and the oil drops at the topmost layer are removed;

the seventh step: the nano fat obtained by centrifugation in the sixth step contains high-concentration fat gel of fat stem cells, so that the synthesis of skin collagen can be promoted, and the effects of improving skin quality, resisting wrinkles and promoting skin regeneration are achieved;

eighth step: and (5) filling the nano fat obtained in the step seven into an ampere bottle, sealing and refrigerating for later use.

In this example, the first centrifugation is controlled at 1500r/2-3min for 4 min.

In this embodiment, the second centrifugation is controlled at 1600r/2-3min at 1500-.

In this example, 1ml of essence was concentrated and extracted from each 10ml of adipose tissue in the secondary purification.

In this embodiment, the main components of the extracellular matrix product in the second step are a composition of collagen, elastin and mucopolysaccharide.

In this embodiment, the mixing ratio of the oil droplets to the nano fat in the fourth step is 1: 4.

The preparation method comprises the steps of obtaining fat, obtaining the nanolaye fat through a mechanical emulsification mode, placing the extracted fat tissue in a high-speed centrifuge for first centrifugal purification, controlling the centrifuge at 1500/2-3min, removing liquid and oil drops, obtaining chyliform nano fat, centrifuging to obtain a product rich in fat stem cells and extracellular matrix, then separating the oil drops in the centrifuged product for later use, mixing the oil drops and the centrifuged nano fat according to a certain ratio, performing second centrifugal extraction, and centrifuging for 8 minutes at 1600/2-3 min to obtain three layers of intermediate fat, namely colloidal svf-gel.

Firstly cutting Coleman fat back and forth, carrying out mechanical emulsification, then changing into nano fat, then adding oil drops collected after preparing the nano fat into the nano fat according to a certain proportion requirement, then carrying out full shaking to change into floccule, starting secondary high-speed centrifugation for full extraction, finally dividing into three layers, removing the liquid at the bottommost layer and the oil drops at the topmost layer, and obtaining the svf-gel in a colloidal form. Svf-ge is obtained by a physical method, and collagenase is not used, so that the method is safer and more efficient.

The preparation of SVF-gel fat gel requires a very complicated procedure, requiring two centrifugations, the first centrifugation being to remove unwanted components such as tumescent fluid, blood cells, etc. from the fat. The second centrifugation removes oil droplets released by the broken mature cells, which if injected into the face, may cause swelling of the face, affecting the recovery period.

To obtain SVF-gel fat gel, thicker tissues in fat must be removed, and repeated boosting by using an injector is needed, wherein the first step is to filter out thick fibers, and the second step is to destroy mature cells which are not easy to survive, so as to improve the fat survival rate. Only the thick fibers in the fat cells are filtered, and the remaining jelly-like fat gel can be smoothly injected through a 27G fine needle (with small wound).

The purified SVF-GEL fat GEL is shaped like hyaluronic acid, is an original ecological physiological combination of extracellular matrix and adipose stem cells in adipose tissues, and contains the extracellular matrix and the environment where fat is dependent on survival. The essence can be concentrated and extracted into 1ml per 10ml of adipose tissue.

The final purpose of the purified SVF-GEL fat GEL is to remove oil from fat tissues obtained by liposuction by a pure physical method so as to achieve the purpose of concentrating stem cells and extracellular matrix, namely, concentrating to obtain essence.

Compared with coleman fat, the SVF-GEL fat GEL prepared by the method has the advantages that the SVF-GEL does not form large oil capsules, and the volume is kept to be more than 80% of the injection amount. SVF-GEL forms a tissue structure that is completely identical to normal fat 90 days after transplantation. That is, the fat gel can grow fat, and has affinity and binding force with skin superior to those of common fat structure.

The SVF-gel fat gel prepared by the method is prepared into SVF in a physical mode, can promote angiogenesis, improve blood circulation and ensure differentiation of adipose-derived stem cells into mature adipocytes. The oil drops in the fat graft are removed, and the inflammatory reaction after fat filling can be reduced. Can promote the synthesis of skin collagen, and achieve the effects of improving skin, resisting wrinkle and promoting skin regeneration. It is possible to fill finer wrinkles completely, which is not done with conventional Coleman fats.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.