阅读说明：本技术 一种猪舍用驱蚊药剂及其制备方法 (Mosquito repellent agent for pigsty and preparation method thereof ) 是由 杨樊 黄琪 余捷 闫春梅 万佳佳 靳洪振 赵青 于 2021-06-23 设计创作，主要内容包括：本发明属于驱蚊药剂技术领域,一种猪舍用驱蚊药剂的制备方法,包括以下制备步骤：S1：提取艾叶菊花提取液；S2：制备精油乳液；S3：将壳聚糖酸溶液加入到乳化好的精油乳液中,制得防蚊微胶囊悬浊液,之后采用水或丙酮清洗3遍,喷雾干燥得到防蚊固体粉末,本发明针对猪的常规养殖环境,使用天然草本成分,对猪对人无毒无害,使用微胶囊包裹成分,实现了活性物质的缓慢释放,有效时间更长,并大幅减少了经皮吸收,原料廉价易得,制作流程简便易行,可以与多种消毒剂联合使用,减少了人力成本。(The invention belongs to the technical field of mosquito repellent medicaments, and discloses a preparation method of a mosquito repellent medicament for a pigsty, which comprises the following preparation steps: s1: extracting folium artemisiae argyi and chrysanthemum extract; s2: preparing an essential oil emulsion; s3: the invention aims at the conventional pig breeding environment, uses natural herbal components, is nontoxic and harmless to pigs, uses microcapsule coating components, realizes the slow release of active substances, has longer effective time, greatly reduces percutaneous absorption, has cheap and easily obtained raw materials, has simple and easy manufacturing flow, can be used together with various disinfectants, and reduces the labor cost.)

1. A preparation method of a mosquito repellent medicament for a pigsty is characterized by comprising the following steps: the preparation method comprises the following preparation steps:

s1: uniformly mixing folium artemisiae argyi and chrysanthemum according to the mass ratio of 1:1, adding water for soaking, then adding ethanol, heating and extracting for 2-4 times, mixing the filtrate obtained by filtering, heating and concentrating to 1g/ml to obtain folium artemisiae argyi and chrysanthemum extracting solution;

s2: adding a synergist and span-80 into the folium artemisiae argyi and chrysanthemum flower extracting solution in the step S1, adding tween-80 into distilled water, stirring to fully dissolve the two, uniformly mixing the two, and shearing and emulsifying the mixture for a certain time by an ultrasonic cell disruptor to form essential oil emulsion;

s3: adding a chitosan acid solution into the emulsified essential oil emulsion, after ultrasonic dispersion, slowly dropwise adding a 10% acetic acid solution into the mixed emulsion to a certain pH value, slowly dropwise adding a wall material water solution into the mixed emulsion, stirring for complex coacervation for a period of time, dropwise adding a small amount of a curing agent for crosslinking and curing for 60min to prepare an anti-mosquito microcapsule suspension, then cleaning for 3 times by adopting water or acetone, and spray-drying to obtain anti-mosquito solid powder.

2. The mosquito repellent agent for the pigsty and the preparation method thereof according to claim 1, characterized in that: the wall material aqueous solution in step S3 is a mixture of sodium dodecylbenzenesulfonate and sodium alginate.

3. The mosquito repellent agent for the pigsty and the preparation method thereof according to claim 2, characterized in that: the volume ratio of the sodium dodecyl benzene sulfonate to the sodium alginate is 1.5-1.8: 1.

4. The mosquito repellent agent for the pigsty and the preparation method thereof according to claim 1, characterized in that: the curing agent is one of glutaraldehyde, TG enzyme and tannic acid.

5. The mosquito repellent agent for the pigsty and the preparation method thereof according to claim 1, characterized in that: the synergist in the step S2 includes two or more of geraniol, methyl salicylate, carveol, and thymol.

6. The mosquito repellent agent for the pigsty and the preparation method thereof according to claim 1, characterized in that: the span-80: tween-80 ═ 2: 1.

7. The mosquito repellent agent for the pigsty is characterized in that: the mosquito repellent for the pigsty comprises the following components: folium artemisiae argyi and chrysanthemum flower extract, a synergist, sodium dodecyl benzene sulfonate, sodium alginate and chitosan polygamic acid.

Technical Field

The invention belongs to the technical field of mosquito repellent agents, and particularly relates to a mosquito repellent agent for a pigsty and a preparation method thereof.

Background

African Swine Fever (ASF) is a virulent infectious disease which is only infected among pigs and is caused by the spread of African Swine Fever Virus (ASFV), and has extremely high morbidity and mortality. Since the first African swine fever epidemic situation is diagnosed in 2018, the epidemic situation develops rapidly, all the country is affected by the epidemic situation, live pigs die greatly, and farmers suffer great economic losses. At present, no effective vaccine has been developed for this disease. For production, the traditional Chinese medicine compound prevention for clearing heat and detoxicating and tonifying healthy qi, such as Yinqiao powder, coptis detoxicating powder and the like, has limited effect on the disease.

The main transmission routes of the African swine fever virus are contact transmission, food transmission and soft tick blood-sucking transmission, and the transmission route can be blocked to effectively inhibit the transmission of diseases. The common pesticide is toxic and harmful, and has short duration. Aiming at the transmission path of the African swine fever virus, a method which effectively blocks the transmission path, is harmless to people and livestock and has low cost is urgently found.

The common commercial mosquito repellent product mainly comprises floral water and mosquito-repellent incense, and has the effects of repelling mosquitoes, removing miliaria, relieving itching and the like. However, the mosquito repellent effect is short, the mosquito repellent needs to be used repeatedly, the effect is seriously reduced when the pigs meet sweat, and the effect is seriously reduced by the actions of rubbing the pigs with each other or rubbing the walls in the actual breeding environment. The mosquito-repellent incense needs open fire, and has potential safety hazard. The electric mosquito repellent has a limited action range, and a breeding environment rarely has enough sockets, so that a moist breeding environment can cause electric leakage danger. The long-acting insect expelling ointment has longer acting time, but the long-acting insect expelling ointment is inconvenient to coat in a breeding environment, and can cause serious reduction of the effect when meeting sweat and the activity of pigs when meeting water.

Disclosure of Invention

The invention aims at the conventional pig breeding environment, uses natural herbal components, is nontoxic and harmless to pigs, uses microcapsule coating components, realizes slow release of active substances, has longer effective time, greatly reduces percutaneous absorption, has cheap and easily obtained raw materials, has simple and easy manufacturing process, can be used together with various disinfectants, and reduces the labor cost.

The technical purpose of the invention is realized by the following technical scheme: a preparation method of a mosquito repellent medicament for a pigsty comprises the following preparation steps:

s1: uniformly mixing folium artemisiae argyi and chrysanthemum according to the mass ratio of 1:1, adding water for soaking, then adding ethanol, heating and extracting for 2-4 times, mixing the filtrate obtained by filtering, heating and concentrating to 1g/ml to obtain folium artemisiae argyi and chrysanthemum extracting solution;

s2: adding a synergist and span-80 into the folium artemisiae argyi and chrysanthemum flower extracting solution in the step S1, adding tween-80 into distilled water, stirring to fully dissolve the two, uniformly mixing the two, and shearing and emulsifying the mixture for a certain time by an ultrasonic cell disruptor to form essential oil emulsion;

s3: adding a chitosan acid solution into the emulsified essential oil emulsion, after ultrasonic dispersion, slowly dropwise adding a 10% acetic acid solution into the mixed emulsion to a certain pH value, slowly dropwise adding a wall material water solution into the mixed emulsion, stirring for complex coacervation for a period of time, dropwise adding a small amount of a curing agent for crosslinking and curing for 60min to prepare an anti-mosquito microcapsule suspension, then cleaning for 3 times by adopting water or acetone, and spray-drying to obtain anti-mosquito solid powder.

The invention is further provided with: the wall material aqueous solution in step S3 is a mixture of sodium dodecylbenzenesulfonate and sodium alginate.

The invention is further provided with: the volume ratio of the sodium dodecyl benzene sulfonate to the sodium alginate is 1.5-1.8: 1.

The invention is further provided with: the curing agent is one of glutaraldehyde, TG enzyme and tannic acid.

The invention is further provided with: the synergist in the step S2 includes two or more of geraniol, methyl salicylate, carveol, and thymol.

The invention is further provided with: the span-80: tween-80 ═ 2: 1.

The mosquito repellent agent for the pigsty is characterized in that: the mosquito repellent for the pigsty comprises the following components: folium artemisiae argyi and chrysanthemum flower extract, a synergist, sodium dodecyl benzene sulfonate, sodium alginate and chitosan polygamic acid.

The invention has the beneficial effects that: .

1. In an acidic solution, amino groups on chitosan molecules are protonated and carry a certain amount of positive charges, another kind of electronegative ions are added, and when the two solutions are mixed in a certain pH value range, the positive charges carried by the chitosan and the negative charges are combined electrostatically, so that the solubility in water is reduced, and the chitosan is separated out from the aqueous solution in a gel form. Sodium dodecyl benzene sulfonate and sodium alginate both exist in the form of anions in an acid solution, can be electrostatically combined with chitosan cations, and are wrapped with plant essential oil to be used as a wall material of the microcapsule to be separated out in the form of gel, so that the solution transmittance is reduced, and the opalescence phenomenon is accompanied.

2. The invention aims at the conventional pig breeding environment, uses natural herbal components, and is non-toxic and harmless to pigs and people. After the disinfectant is used, the disinfectant not only has the effects of repelling mosquitoes and killing insects and cutting off the transmission path of African swine fever, but also can form an air drug molecular film layer indoors, volatile substances contained in the disinfectant can form a natural disinfection air curtain in the air of a pigsty, and a drug film is formed on mucous membranes of a nasal throat and a trachea of a pig, which are most prone to bacteria and viruses accumulation, so that the effects of sterilizing, killing viruses and preventing and treating respiratory infectious diseases are achieved. And the raw materials are cheap and easy to obtain, and the preparation flow is simple and easy to implement.

3. The invention adds the synergist during preparation, has obvious synergistic effect on insect expelling effect by adopting the synergist, can reduce the using amount of the added components, is safe and non-irritant to livestock and human bodies by taking the plant essential oil monomer compound as the synergistic component, is easy to decompose without residue, has no pollution to the environment, has various target action modes, can delay the generation of drug resistance of pests, and meets the requirements of people on green, safe and environment-friendly sanitation and insect killing.

4. The invention adopts natural essential oil as a core material, and natural polymer material chitosan is respectively subjected to complex coacervation with sodium alginate and sodium dodecyl benzene sulfonate as a wall material to prepare the mosquito-repellent microcapsule, so that the effective mosquito-repellent component in the microcapsule has a slow-release effect, the volatilization rate and the transdermal absorption rate of the mosquito-repellent agent are reduced, and the effective evasion time of the mosquito-repellent agent is prolonged.

5. Aiming at the conventional pig breeding environment, the natural herbal ingredients are used, so that the pig feed is nontoxic and harmless to people, the microcapsule-coated ingredients are used, the slow release of active substances is realized, the effective time is longer, and the percutaneous absorption is greatly reduced; the raw materials are cheap and easy to obtain, and the preparation flow is simple and easy to implement; can be used together with various disinfectants, thereby reducing the labor cost.

Detailed Description

The technical solutions in the examples will be clearly and completely described below. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without any inventive step, are within the scope of the present invention.

Comparative example:

a preparation method of a mosquito repellent medicament for a pigsty comprises the following preparation steps:

s1: uniformly mixing folium artemisiae argyi and chrysanthemum according to the mass ratio of 1:1, adding water for soaking, then adding ethanol, heating and extracting for 3 times, mixing the filtrates obtained by filtering, and heating and concentrating to 1g/ml to obtain the folium artemisiae argyi and chrysanthemum extracting solution.

S2: adding span-80 into the folium artemisiae argyi and chrysanthemum flower extracting solution in the step S1, adding tween-80 into distilled water, stirring to fully dissolve the mixture, uniformly mixing the mixture and the distilled water, and shearing and emulsifying the mixture for a certain time by an ultrasonic cell disruptor to form the essential oil emulsion.

S3: adding a chitosan acid solution into the emulsified essential oil emulsion, after ultrasonic dispersion, slowly dropwise adding a 10% acetic acid solution into the mixed emulsion to a certain pH value, slowly dropwise adding a mixed aqueous solution of sodium dodecyl benzene sulfonate and sodium alginate into the mixed emulsion, stirring for re-coagulation for a period of time, dropwise adding a small amount of glutaraldehyde for crosslinking and curing for 60min to prepare an anti-mosquito microcapsule suspension, then cleaning for 3 times by using water or acetone, and spray drying to obtain anti-mosquito solid powder.

Example 1:

a preparation method of a mosquito repellent medicament for a pigsty comprises the following preparation steps:

s1: uniformly mixing folium artemisiae argyi and chrysanthemum according to the mass ratio of 1:1, adding water for soaking, then adding ethanol, heating and extracting for 3 times, mixing the filtrates obtained by filtering, and heating and concentrating to 1g/ml to obtain the folium artemisiae argyi and chrysanthemum extracting solution.

S2: adding geraniol, methyl salicylate and span-80 into the folium artemisiae argyi and chrysanthemum extracting solution in the step S1, adding tween-80 into distilled water, stirring to fully dissolve the materials, uniformly mixing the two materials, and shearing and emulsifying the mixture for a certain time by an ultrasonic cell disruptor to form the essential oil emulsion.

S3: adding a chitosan acid solution into the emulsified essential oil emulsion, after ultrasonic dispersion, slowly dropwise adding a 10% acetic acid solution into the mixed emulsion to a certain pH value, slowly dropwise adding a mixed aqueous solution of sodium dodecyl benzene sulfonate and sodium alginate into the mixed emulsion, stirring for re-coagulation for a period of time, dropwise adding a small amount of glutaraldehyde for crosslinking and curing for 60min to prepare an anti-mosquito microcapsule suspension, then cleaning for 3 times by using water or acetone, and spray drying to obtain anti-mosquito solid powder.

Example 2:

a preparation method of a mosquito repellent medicament for a pigsty comprises the following preparation steps:

s1: uniformly mixing folium artemisiae argyi and chrysanthemum according to the mass ratio of 1:1, adding water for soaking, then adding ethanol, heating and extracting for 2 times, mixing the filtrates obtained by filtering, and heating and concentrating to 1g/ml to obtain the folium artemisiae argyi and chrysanthemum extracting solution.

S2: adding methyl salicylate, carveol and span-80 into the folium artemisiae argyi and chrysanthemum extracting solution in the step S1, adding tween-80 into distilled water, stirring to fully dissolve the mixture, uniformly mixing the mixture and the distilled water, and shearing and emulsifying the mixture for a certain time by an ultrasonic cell disruptor to form essential oil emulsion.

S3: adding a chitosan acid solution into the emulsified essential oil emulsion, after ultrasonic dispersion, slowly dropwise adding a 10% acetic acid solution into the mixed emulsion to a certain pH value, slowly dropwise adding a mixed aqueous solution of sodium dodecyl benzene sulfonate and sodium alginate into the mixed emulsion, stirring for re-coagulation for a period of time, dropwise adding a small amount of TG enzyme for crosslinking and curing for 60min to prepare an anti-mosquito microcapsule suspension, then cleaning for 3 times by using water or acetone, and spray drying to obtain anti-mosquito solid powder.

Example 3:

a preparation method of a mosquito repellent medicament for a pigsty comprises the following preparation steps:

s1: uniformly mixing folium artemisiae argyi and chrysanthemum according to the mass ratio of 1:1, adding water for soaking, then adding ethanol, heating and extracting for 4 times, mixing the filtrates obtained by filtering, and heating and concentrating to 1g/ml to obtain the folium artemisiae argyi and chrysanthemum extracting solution.

S2: adding carveol, thymol and span-80 into the folium artemisiae argyi and chrysanthemum extracting solution in the step S1, adding tween-80 into distilled water, stirring to fully dissolve the materials, uniformly mixing the two materials, and shearing and emulsifying the mixture for a certain time by an ultrasonic cell disruptor to form the essential oil emulsion.

S3: adding a chitosan acid solution into the emulsified essential oil emulsion, after ultrasonic dispersion, slowly dropwise adding a 10% acetic acid solution into the mixed emulsion to a certain pH value, slowly dropwise adding a mixed aqueous solution of sodium dodecyl benzene sulfonate and sodium alginate into the mixed emulsion, stirring and re-coagulating for a period of time, dropwise adding a small amount of tannic acid for crosslinking and curing for 60min to prepare an anti-mosquito microcapsule suspension, then cleaning for 3 times by using water or acetone, and spray drying to obtain anti-mosquito solid powder.

The insecticide is prepared by the comparative example and the anti-mosquito solid powder prepared in the examples 1 to 3, the poisoning effect on culex pipiens pallens is tested, meanwhile, the insecticide content for 3 hours is measured, the percentage of the insecticide content after 5 hours is obtained, and the knockdown rate in the examples 1 to 3 is compared with the knockdown rate in the comparative example, so that the mosquito knockdown synergistic coefficient is obtained.

TABLE 1 knock-down synergistic Activity assay of insect protected solid powder against Culex pipiens pallens

It can be seen that although the knockdown mortality rate of culex pipiens pallens is lower than that of the embodiment in the comparative example, the mosquito knockdown rate is still higher, and compared with the comparative example without the synergist in the embodiments 1 to 3, the synergist has an obvious synergistic effect on the insecticide, and the natural high polymer material chitosan is respectively re-agglomerated with sodium alginate and sodium dodecyl benzene sulfonate to form a wall material to prepare the mosquito-repellent microcapsule, so that the effective mosquito-repellent component in the interior can have a slow-release effect, the volatilization rate and the transdermal absorption rate of the mosquito-repellent agent are reduced, and the effective repelling time of the mosquito-repellent agent is prolonged.