阅读说明：本技术 一种眉斑并脊天牛实验种群的高效建立方法及其饲养装置 (Efficient establishing method and feeding device for experimental population of Sucus bubali ) 是由 董子舒 陆慧洁 郑霞林 王小云 陆温 李俊 杨娟 李洪汉 李岳诗 唐荣 黎海霖 于 2020-06-19 设计创作，主要内容包括：本发明涉及眉斑并脊天牛饲养技术领域,公开了一种眉斑并脊天牛实验种群的高效建立方法及其饲养装置,方法包括虫源采集、饲料采集、出木--交配、孵化--低龄幼虫、低龄幼虫--出木和成虫出木等步骤。本发明涉及的眉斑并脊天牛各虫态饲养装置构造简单,成本低廉,操作简易,循环使用；本发明具备规模养殖天牛,每年能够繁殖4-5个世代,在人工控温下全年均可以繁殖,既可满足试验研究、昆虫膳食需要,还可制作色彩鲜艳、体型优美、极具观赏价值的工艺品的优点,解决了现有的眉斑并脊天牛室内饲养方法其幼虫接种过程步骤相对复杂,费时费力,成虫收集过程效率较低和统计眉斑并脊天牛幼虫的化蛹率及出木率不方便,增加了实验难度的问题。(The invention relates to the technical field of breeding of David brows and longicorn, and discloses a method for efficiently establishing experimental population of the David brows and longicorn and a breeding device thereof. The insect-state breeding device for the longicorn mottles with the brow spots and the spine has the advantages of simple structure, low cost, simplicity and easiness in operation and recycling; the method has the advantages that the longicorn can be bred in a large scale, 4-5 generations can be bred every year, the longicorn can be bred all the year under the condition of manual temperature control, the dietary requirements of experimental research and insects can be met, artworks which are bright in color, beautiful in body shape and high in ornamental value can be manufactured, and the problems that the larva inoculation process is relatively complex in steps, time and labor are wasted, the efficiency of the adult collection process is low, the pupation rate and the emergence rate of the longicorn larva are inconvenient to count, and the experimental difficulty is increased in the existing indoor breeding method of the longicorn and carina carica are solved.)

1. A method for efficiently establishing experimental population of Sucus bubali, which is characterized by comprising the following steps: the method comprises the following steps:

firstly, collecting insect sources, namely collecting longicorn seedlings by purchasing or going to the field;

secondly, collecting feed, namely cutting and reserving fresh branches when the kapok tree is trimmed;

thirdly, wood production-mating, namely identifying female and male adults produced by wood production, separately placing the adults in a feeding cup, and pairing the female and male adults in the pre-mating stage and laying eggs;

fourthly, hatching-low-age larvae, collecting the ceiba branches with spawning carved grooves once every 3 to 5 days, and placing the ceiba branches in a sterilized plastic collecting bag;

fifthly, breeding the low-age larvae, namely yielding wood, breeding by using a larva breeding box, preparing kapok branches with the length of 5-8cm and the diameter of 3-5cm in advance, inserting a screwdriver into xylem of the branches when the larvae are inoculated, pricking out a groove with the depth of 3-5cm, placing the low-age larvae into the groove, then respectively placing the branches with the larvae into larva breeding tubes with the length of 8-10cm and the diameter of 5-8cm, and uniformly placing the breeding tubes on bases of the breeding box until the imagoes generate wood;

and sixthly, enabling the adult insects to grow out of the wood, and circularly carrying out subculture according to the steps.

2. The method for efficiently establishing the experimental population of the Georgia amabilis according to claim 1, wherein the experimental population comprises the following steps: in the first step, the collection method comprises the steps of finding a harmful plant in a Goniobia dorsalis-Shaw area with longicorn as a harmful ceiba forest area, removing completely withered parts at the top, cutting branches and stems at the bottom into 50-60cm wood sections, placing the wood sections in larva feeding boxes made of shading materials, placing 8-10 wood sections in each larva feeding box, keeping spraying a proper amount of clear water every week for moisturizing, covering a box cover, keeping feeding the larva in a feeding environment at 25 +/-1 ℃ for 45-50 days, and enabling adults to emerge.

3. The method for efficiently establishing the experimental population of the Georgia amabilis according to claim 1, wherein the experimental population comprises the following steps: in the first step, the adult insects crawl in a light-transmitting collecting box at the upper side of the insect breeding box due to the characteristics of land-bearing property and phototropism.

4. The method for efficiently establishing the experimental population of the Georgia amabilis according to claim 1, wherein the experimental population comprises the following steps: in the first step, collecting adult wood-out insects which climb into a collecting box, selecting female individuals with the weight of more than 0.2g and male individuals with the weight of more than 0.1g to be respectively placed in insect breeding bottles, and breeding the adult wood-out insects for later use by using small branches of ceiba.

5. The method for efficiently establishing the experimental population of the Georgia amabilis according to claim 1, wherein the experimental population comprises the following steps: and in the second step, the branches are placed in a freezer for fresh preservation and are left in the eyebrow spots and bred for the experimental population of the longhorn beetles for later use.

6. The method for efficiently establishing the experimental population of the Georgia amabilis according to claim 1, wherein the experimental population comprises the following steps: in the third step, the spawning branches and the feeding branch cup seats are arranged in the feeding cup, so that the efficiency of collecting the spawning branches in the later period is greatly improved.

7. The method for efficiently establishing the experimental population of the Georgia amabilis according to claim 1, wherein the experimental population comprises the following steps: in the fourth step, the eggs are placed for 10-14 days at 25 + -1 deg.C.

8. The method for efficiently establishing the experimental population of the Georgia amabilis according to claim 1, wherein the experimental population comprises the following steps: in the fifth step, as the ground can climb to the light-transmitting tube cover, the adults in the cover can be directly collected when seeing the insects, and a collecting drawer is arranged at the bottom of each feeding box, so that food residues and larva excrement can be conveniently and uniformly cleaned.

9. The utility model provides a device of raising of experimental population of crow's spot and spine longicorn which characterized in that: the longicorn larva breeding device comprises a longicorn larva breeding box (1), wherein a breeding box body (101) is arranged inside the longicorn larva breeding box (1), a plurality of battens (102) are arranged inside the breeding box body (101), and an adult collecting box (103) is arranged inside the breeding box body (101);

the longicorn adult rearing cup (2), the longicorn adult rearing cup (2) comprises a spawning branch cup seat (201), a feeding branch cup seat (202) is installed at the top of the spawning branch cup seat (201), a cup cover (203) is installed at the top of the spawning branch cup seat (201), a handle (204) is installed at the top of the cup cover (203), and air holes (205) are formed in the surface of the cup cover (203);

box (3) is raised to longicorn larva, longicorn larva is raised box (3) and is including collecting steamer tray (301), the top of collecting steamer tray (301) is installed and is raised box base (302), the internally mounted who raises box base (302) has the larva to raise pipe (303), the top that pipe (303) was raised to the larva is installed the adult through the hinge buckle and is collected lid (304).

10. The raising apparatus of experimental herd of crow's spot and spine longicorn according to claim 9, characterized in that: the side of raising box (101) is the light screen, and the top of adult collecting lid (304) and the bottom of larva raising pipe (303) all install screen cloth (4).

Technical Field

The invention relates to the technical field of breeding of longicorn mottles, in particular to a method for efficiently establishing experimental population of longicorn mottles and a breeding device thereof.

Background

Geotrichum brotherum, also known as Geotrichum brotherum, belongs to the order Coleoptera, family Annonaceae, subfamily Annona, and genus Annona. The longhorn beetles are distributed abroad in Vietnam, Philippine and Laos, and are distributed domestically in provinces such as Guangdong, Guangxi, Yunnan, Guizhou, Hainan and hong Kong, and are important pests of kapok trees [2 ]. Biological control is one of the most effective measures for the comprehensive control of the longicorn, the large-scale release of the tubificidae swollen legs is a control method with good biological control effect and high efficiency for the longicorn, but the problem of population degradation cannot be solved because the intermediate hosts are non-longicorn borers, tenebrio molitor and the like; although the anthurium andraeanum, Szechwan scleroderma drumstick, and the like have a good natural control effect on the longicorn, in order to solve the problem of intermediate hosts of parasitic natural enemies of the longicorn, some artificial large-scale feeding methods have been developed.

At present, several indoor raising techniques for the cerasus eyebroworum and the carina have been reported at home and abroad. However, they all have several disadvantages:

1. the steps of the larva inoculation process are relatively complex, and time and labor are wasted;

2. the efficiency of the adult collecting process is low;

3. the pupation rate and the wood yield of the brow spot and longhorn beetle larvae are inconvenient to count, and the experiment difficulty is increased.

Disclosure of Invention

The invention aims to provide a method for efficiently establishing experimental population of the longicorn mottles and the breeding device thereof, which has the advantages of breeding longicorn on a large scale, breeding 4-5 generations each year, breeding all the year round under artificial temperature control, meeting experimental research and insect diet requirements, manufacturing artware with bright color, beautiful body and high ornamental value, and solving the problems that the existing indoor breeding method of the longicorn mottles and the longicorn has relatively complicated larva inoculation process steps, wastes time and labor, has low efficiency in adult collection process, is inconvenient in counting pupation rate and emergence rate of the longicorn mottles and the longicorn larvae, and increases experimental difficulty.

In order to achieve the purpose, the invention provides the following technical scheme: a method for efficiently establishing experimental population of Georgia amabilis comprises the following steps:

firstly, collecting insect sources, namely collecting longicorn seedlings by purchasing or going to the field;

secondly, collecting feed, namely cutting and reserving fresh branches when the kapok tree is trimmed;

thirdly, wood production-mating, namely identifying female and male adults produced by wood production, separately placing the adults in a feeding cup, and pairing the female and male adults in the pre-mating stage and laying eggs;

fourthly, hatching-low-age larvae, collecting the ceiba branches with spawning carved grooves once every 3 to 5 days, and placing the ceiba branches in a sterilized plastic collecting bag;

fifthly, breeding the low-age larvae, namely yielding wood, breeding by using a larva breeding box, preparing kapok branches with the length of 5-8cm and the diameter of 3-5cm in advance, inserting a screwdriver into xylem of the branches when the larvae are inoculated, pricking out a groove with the depth of 3-5cm, placing the low-age larvae into the groove, then respectively placing the branches with the larvae into larva breeding tubes with the length of 8-10cm and the diameter of 5-8cm, and uniformly placing the breeding tubes on bases of the breeding box until the imagoes generate wood;

and sixthly, enabling the adult insects to grow out of the wood, and circularly carrying out subculture according to the steps.

Preferably, in the first step, the collecting method comprises the steps of finding a harmful plant in a Goniobia dorsalis-Shaw and Tianniu bombax forest area, removing a completely withered part at the top, cutting a lower branch part into 50-60cm wood sections, placing the wood sections in larva breeding boxes made of shading materials, placing 8-10 wood sections in each larva breeding box, keeping spraying a proper amount of clear water every week for moisture preservation, covering a box cover, keeping breeding for 45-50 days at the temperature of 25 +/-1 ℃, and then enabling adults to emerge.

Preferably, in the first step, the adult insects crawl in a light-transmitting collecting box at the upper side of the insect breeding box due to the characteristics of land-bearing property and phototropism.

Preferably, in the first step, the grown-out adult wood worms which climb into the collecting box are collected, female individuals with the weight of more than 0.2g and male individuals with the weight of more than 0.1g are selected to be respectively placed in insect breeding bottles and are bred by using small ceiba branches for standby.

Preferably, in the second step, the branches are placed in a freezer for fresh preservation, and are left in the eyebrow spots and bred for the experimental population of the longhorn beetles for standby.

Preferably, in the third step, the spawning branches and the feeding branch cup seats are arranged in the feeding cup, so that the spawning branch collection efficiency in the later period is greatly improved.

Preferably, in said fourth step, the eggs are placed for 10-14 days at 25 ± 1 ℃ in ambient conditions.

Preferably, in the fifth step, as the ground can climb to the light-transmitting tube cover, the adults in the cover can be directly collected when seeing the insects, and a collecting drawer is arranged at the bottom of each feeding box, so that food residues and larva excrement can be conveniently and uniformly cleaned.

The experimental population raising device for the longicorn brother with the brow spots comprises a longicorn larva raising box, wherein a raising box body is arranged inside the longicorn larva raising box, a plurality of battens are arranged inside the raising box body, and an adult collecting box is arranged inside the raising box body;

the longicorn adult rearing cup comprises a spawning branch cup seat, a feeding branch cup seat is installed at the top of the spawning branch cup seat, a cup cover is installed at the top of the spawning branch cup seat, a handle is installed at the top of the cup cover, and air holes are formed in the surface of the cup cover;

box is raised to longicorn larva, box is raised including collecting the steamer tray to longicorn larva, the top of collecting the steamer tray is installed and is raised the box base, the internally mounted who raises the box base has the larva to raise the pipe, the top that the pipe was raised to the larva is installed the adult through the hinge buckle and is collected the lid.

Preferably, the side of raising the box is the light screen, and the screen cloth is all installed to the top of adult collecting cover and the bottom of larva raising pipe.

Compared with the prior art, the invention has the following beneficial effects:

(1) the insect-state breeding device for the longicorn mottles with the brow spots and the spine has the advantages of simple structure, low cost, simplicity and easiness in operation and recycling;

(2) compared with the prior art, the emergence rate of the method is higher than that of most breeding methods on the premise of ensuring the absolute advantages of the average weight, pupation rate and total survival rate of the grown-out imagoes;

(3) the eyebrow spot and spine longicorn larva breeding box adopted by the invention has the specification of 60 × 50 × 55cm³The periphery and the bottom surface of the special collecting box are both lightproof foams, the top surface of the foam cover is provided with a proper amount of pricked holes (the diameter is 0.1cm) for ventilation, the middle part of the foam cover is circularly cut into a worm outlet hole with the diameter of 5.0cm, the worm outlet hole is covered with a special adult collecting box (the upper diameter is 15cm, the lower diameter is 8cm, and the height is 12cm) pricked with ventilation holes, and the whole material of the special collecting box is a transparent PVC plate. The device fully moisturizes, has guaranteed the survival rate and the rate of producing trees of open-air worm source. Meanwhile, the device also makes full use of the property that the grown-out imagoes are back to the ground and light, and greatly improves the collection efficiency of the grown-out imagoes in the field;

(4) according to the invention, the adult brow spot and spine longicorn breeding cup is adopted, a cup-shaped cover is additionally arranged at the top of the cup-shaped cover, a proper amount of air holes (the diameter is 0.1cm) are tied on the cup-shaped cover, and a spawning branch cup seat and a feeding branch cup seat are designed at the bottom of the cup according to the life habit that the brow spot and spine longicorn preferentially spawns on a thick branch and feeds a thin branch under the condition that the thick branch and the thin branch exist;

(5) according to the invention, the brow spot and longhorn beetle larva feeding box is used for uniformly accommodating the insect feeding pipes, so that the space is saved, meanwhile, larva food residues and excrement are uniformly collected, the integral cleaning management is convenient, and the workload is saved;

(6) according to the brow spot and longhorn beetle larva feeding pipe, the workload of independently cutting the egg-containing notch groove in the transfer method with the skin layer is reduced, the larvae are prevented from being mutually bitten in the same space under the condition that the food intake is guaranteed by the independent larvae, meanwhile, the characteristic that the brow spot and longhorn beetle eggs are back to the ground after being wooded is utilized, a pipe cover with a hinge buckle is provided at the pipe opening, and the collection of wooded adults is facilitated.

Drawings

FIG. 1 is a schematic structural view of a longicorn larva raising box according to the present invention;

FIG. 2 is a schematic structural view of a longicorn adult rearing cup according to the present invention;

FIG. 3 is a schematic structural view of a longicorn larva raising box according to the present invention;

FIG. 4 is a schematic view of the structure of a larval rearing tube of the present invention.

In the figure: 1. a longicorn larva breeding box; 101. a feeding box body; 102. wood strips; 103. an adult collecting box; 2. adult longicorn feeding cups; 201. spawning branch cup seats; 202. taking a eating branch cup seat; 203. a cup cover; 204. a handle; 205. air holes are formed; 3. a longicorn larva breeding box; 301. a collection drawer; 302. a feeding box base; 303. a larva raising pipe; 304. adult collecting cover; 4. and (4) screening.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

A method for efficiently establishing experimental population of Georgia amabilis comprises the following steps:

firstly, collecting insect sources, wherein the longicorn seedlings can be bought or collected in the field; the collecting method comprises the steps of finding harmful plants in a Goniobia carinata forest area, removing completely withered top parts, cutting the lower branch parts into wood sections with the length of about 50cm, placing the wood sections in larva breeding boxes made of shading materials, placing 10 wood sections in each larva breeding box, spraying a little clear water every week for moisture preservation, covering a special box cover, breeding for about 45 days at the temperature of 25 +/-1 ℃, enabling adults to emerge through eclosion, crawling in a light-transmitting collecting box on the upper side of the larva breeding box due to the characteristics of land property and phototropism of the adults, collecting adult worms which climb to the wood in the collecting box, selecting female individuals with the weight of more than 0.2g and male individuals with the weight of more than 0.1g, placing the female individuals and the male individuals into insect breeding bottles respectively, and breeding for later use by small branches of kapok.

Secondly, collecting feed, contacting related nursery gardens or related greening units, and cutting and retaining fresh branches when the kapok trees are trimmed; placing the branches in a freezer for fresh preservation, and keeping the branches in the eyebrow spots and feeding the experimental population of the longhorn beetles for later use.

Thirdly, wood production-mating, namely identifying female and male adults produced by wood production, separately placing the adults in a feeding cup, and pairing the female and male adults in the pre-mating stage and laying eggs; the spawning branches and the feeding branch cup seats are arranged in the feeding cup, so that the efficiency of collecting the spawning branches in the later period is greatly improved.

Fourthly, hatching-larvae of low age, collecting the ceiba branches with spawning carved grooves, collecting the ceiba branches once every 3 days, and placing the ceiba branches in a sterilized plastic collecting bag; the eggs were allowed to stand for 10-14 days at 25 + -1 deg.C.

Fifthly, breeding the low-age larvae with wood, breeding the low-age larvae with a larva breeding box, preparing kapok branches with the length of about 5cm and the diameter of about 3cm in advance, inserting a screwdriver into xylem of the branches when the larvae are inoculated, pricking out grooves with the depth of 3cm, placing the low-age larvae into the grooves, then respectively placing the branches with the larvae into larva breeding pipes (with the length of 8cm and the diameter of 5cm), and uniformly placing the breeding pipes on a base of the breeding box until the imagoes feather out wood; because the ground can climb to the light-transmitting tube cover, the adults in the cover can be directly collected when the adults are seen, and a collecting drawer is arranged at the bottom of each feeding box, so that food residues and larva excrement can be conveniently and uniformly cleaned.

And sixthly, enabling the adult insects to grow out of the wood, and circularly carrying out subculture according to the steps.

Referring to fig. 1-4, the experimental herd of the brow-spot-combined longicorn is a raising device, which comprises a longicorn larva raising box 1, wherein the inside of the longicorn larva raising box 1 comprises a raising box body 101, the side surface of the raising box body 101 is a light screen, a plurality of battens 102 are arranged inside the raising box body 101, and an adult collecting box 103 is arranged inside the raising box body 101;

the longicorn adult rearing cup 2 comprises a spawning branch cup base 201, a feeding branch cup base 202 is installed at the top of the spawning branch cup base 201, a cup cover 203 is installed at the top of the spawning branch cup base 201, a handle 204 is installed at the top of the cup cover 203, and air holes 205 are formed in the surface of the cup cover 203;

box 3 is raised to longicorn larva, and longicorn larva is raised box 3 and is installed and raise box base 302 including collecting steamer tray 301, the top of collecting steamer tray 301, and the internally mounted who raises box base 302 has the larva to raise pipe 303, and the top that pipe 303 was raised to the larva is installed the adult through the hinge buckle and is collected lid 304, and the top that lid 304 was collected to the adult and the bottom that pipe 303 was raised to the larva all install screen cloth 4.

It should be noted that: in the process of raising the eyebrow spot and spine longicorn in each insect state, setting indoor raising environmental conditions to be kept as follows: the temperature is 25 +/-1 ℃ and the humidity is 75 +/-5%.

Test data table of the invention:

TABLE 1 Effect of different rearing methods on the survival rate, pupation rate and emergence rate of Limoid beetle larvae

TABLE 2 Effect of different feeding methods on the weight and sex ratio of male and female adults of Georgia amabilis

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.