阅读说明：本技术 一种可观测性眉斑并脊天牛幼虫饲养方法及其装置 (Method and device for feeding observable brow-spot-combining longicorn larvae ) 是由 董子舒 陆慧洁 王小云 郑霞林 陆温 李岳诗 李洪汉 巫辅民 唐荣 黎海霖 杨娟 于 2020-06-19 设计创作，主要内容包括：本发明专利公开了一种可观测性眉斑并脊天牛幼虫饲养方法及其装置,其饲养方法包括如下步骤：根据饲养规模选取一定平方的饲养室,且室内安装一台排风扇保持通风,安装一台冷暖空调设备保持实验温度；实验种群眉斑并脊天牛,选择交配后过了产卵前期的雌虫,新鲜的木棉枝条与雄虫共同饲养于养虫瓶内；本发明的方法规模养殖眉斑并脊天牛,每年能够繁殖4-5个世代,在人工控温下全年均可以繁殖,本发明的眉斑并脊天牛各虫态观察饲养装置结构简单,成本低廉,简便实用,适用范围广,为弄清眉斑并脊天牛生物学特性、发育生物学和不同虫态过冷却点及结冰点等特性等相关科学实验,提供了大量实验需求的不同虫态发育程度的供试虫源。(The invention discloses a method and a device for feeding observable milbemyces eyebrows and longhorned beetle larvae, wherein the feeding method comprises the following steps: selecting a certain square breeding room according to the breeding scale, installing an exhaust fan in the breeding room to keep ventilation, and installing a cooling and heating air conditioning device to keep the experimental temperature; experimental population of the zornia cantonensis, selecting female worms which pass through the stage before spawning after mating, and feeding fresh ceiba branches and male worms in a worm breeding bottle together; the eyebrow spot and longhorn beetle scale culture method can breed 4-5 generations every year, and can breed all the year round under artificial temperature control, the eyebrow spot and longhorn beetle insect state observation and breeding device has the advantages of simple structure, low cost, simplicity, practicality and wide application range, and provides a large amount of insect sources for testing different insect state development degrees required by experiments for relevant scientific experiments such as the biological characteristics, the developmental biology, different insect state supercooling points, freezing points and the like of the eyebrow spot and the longhorn beetle.)

1. A method for feeding observable Meishan-Sharpe-Daphniphyllus larvae is characterized in that: the breeding method comprises the following steps:

(1) arranging a breeding room: selecting a feeding room according to the feeding scale, installing an exhaust fan in the feeding room to keep ventilation, and installing a cooling and heating air conditioning device to keep the experimental temperature;

(2) insect source collection: experimental population brothers and longicorn, selecting female worms which pass through the stage of laying eggs before mating, feeding fresh ceiba branches and male worms in an insect breeding bottle together, taking out the branches after 3-5 days, selecting effective laying egg notches at the stripped phloem, repeating the operation, replacing and collecting the ceiba branches with the laying egg notches once every three days, and then placing the ceiba branches in different larva feeding grids respectively;

(3) collecting feed: when the ceiba greening trees are pruned, the pruned fresh branches are collected, refrigerated and kept for standby feeding;

(4) and (3) low-age larvae: putting the egg-containing bark without taking eggs in the device for 5-8 days, hatching most of the eggs of the brow spot branchlet and longhorn beetle, entering 1-year larvae or taking insects for observation experiment, observing the notch and the periphery of the bark to form crumbs in 10-14 days, indicating that the vitality of the larvae is enhanced and entering 2-year larvae after entering 2-year, stripping the bark to remove the 2-year larvae, and facilitating the observation and breeding at the later time;

(5) old larvae: feeding larvae of 2 th instar into 3 th instar after molting on 15 th-20 th day, feeding larvae of 3 rd instar into 4 th instar after molting on 21 th-27 th day, and feeding food to help the larvae to grow;

(6) pupating: the pupal stage is started on day 32-38, the pupal stage is 12-15 days, no feed is added, and the observation experiment of pupal needs to be prepared in advance in the mature larva stage;

(7) adult: after eclosion, adults can wait for 5-6 days in a pupa chamber made of phloem residues in a larva feeding grid, and finally climb to the top end of the feeding grid due to the phototropism of the back of the adults, the adult is beaten before a strip-shaped drawer is drawn out due to the false death characteristic, the adults are directly caught in the feeding grid by tweezers after being pulled out, and after the adults come out of the tree, the adults are continuously fed in a successive transfer mode according to the steps (2) - (6).

2. The method for rearing observable milbejean beetle larvae according to claim 1, wherein the method comprises the steps of: in the step (1), the area of the feeding chamber is 20-25m²And a stainless steel screen window is arranged to prevent the insects from escaping, and the indoor feeding environmental conditions are kept at the temperature of 25 +/-1 ℃ and the humidity of 75 +/-5%.

3. The method for rearing observable milbejean beetle larvae according to claim 1, wherein the method comprises the steps of: in the step (2), two branches of the ceiba branches with the diameter of 1-2cm and one branch with the diameter of 3-5cm are selected, and the bark surrounding the oviposition notch is cut into 2.0 multiplied by 2.0cm and taken out.

4. The method for rearing observable milbejean beetle larvae according to claim 1, wherein the method comprises the steps of: in the step (2), when the phloem is peeled, the branch is firstly abutted against the xylem from one end to the other end of the branch by using a blade, and the phloem and the xylem are peeled and peeled.

5. The method for rearing observable milbejean beetle larvae according to claim 1, wherein the method comprises the steps of: in the step (4), a proper amount of phloem fragments or strips should be periodically supplemented in the stage of the young larvae to cover the larva bodies so as to ensure sufficient food.

6. The method for rearing observable milbejean beetle larvae according to claim 1, wherein the method comprises the steps of: in the step (5), the fed food is ensured to cover the insect body by more than 1cm, and a 1-2cm ceiba xylem strip is added.

7. The method for rearing observable milbejean beetle larvae according to claim 1, wherein the method comprises the steps of: in the step (6), the preparation work needs to take out the larva entering the prepupa and put the larva into a 10-15mL medium-sized finger-type pipe padded with toilet paper to be pupated.

8. The utility model provides an observable eyebrow spot and spine longicorn larva raising device, includes raises box (1), its characterized in that: raise box (1) inboard upper and lower side and be provided with bar drawer (2) and clearance drawer (3) respectively, the inside of bar drawer (2) is provided with the same check (4) of raising in a plurality of spaces, the inboard bottom of bar drawer (2) is provided with gauze (5), the front end fixedly connected with handle (6) of bar drawer (2), the place ahead fixedly connected with handle (7) of clearance drawer (3).

9. The observable brown-spotted grouper larva raising apparatus according to claim 8, wherein: the top of the feeding box (1) is provided with a plurality of vent holes (8), the vent holes (8) are distributed in a matrix manner, and a humidifying cup (9) is arranged at the center of the bottom of the inner side of the cleaning drawer (3).

10. The observable brown-spotted grouper larva raising apparatus according to claim 9, wherein: the breeding box (1), the strip-shaped drawer (2) and the cleaning drawer (3) are all made of light-tight plastics, the aperture of the vent hole (8) is 0.2cm, the number of the strip-shaped drawers (2) is ten, the specifications of the strip-shaped drawers are 30 multiplied by 10cm, the specification of the breeding grid (4) is 3.0 multiplied by 4.0cm, the specification of the cleaning drawer (3) is 30 multiplied by 5cm, and the specification of the humidifying cup (9) is 3.0 multiplied by 3.0 cm.

Technical Field

The invention relates to the technical field of longicorn breeding, in particular to a method and a device for breeding longicorn larvae with observable eyebrow streaks and ridges.

Background

Geranium broilense, also known as Geranium broilense, belongs to Coleoptera, Annonaceae, Annona, and Annona. The longhorn beetles are distributed abroad in Vietnam, Philippine and Laos, and are mainly distributed domestically in provinces such as Guangdong, Guangxi, Yunnan, Guizhou, Hainan and hong Kong, and are important stem borers of kapok trees. The brow-spot-combining longhorn beetles have the characteristics of dry larva, hidden life, generation of more than one generation in one year, overlapping generation and the like, so that the research on the biology, the physiological ecology and the pest control of the brow-spot-combining longhorn beetles is significant.

The artificial feeding of the insects is an important main city part for insect research at home and abroad, and the technology breaks through the strict limitation of seasons and host factors on the time regulation and control of scientific research work, and ensures that experimental insects exist in scientific researches such as insect biological research, insect physiological research, pest control research and the like throughout the year. In addition, the experimental population has the characteristics of more consistent physiological standards, high-efficiency amplification and the like, and the smooth progress of various scientific research works is fully ensured. The longicorn is used as an important pest in the field of agriculture and forestry, belongs to the coleoptera with the largest number of insects, and is rich in species. However, the concealment of the longicorn larva life and the characteristics of long duration and few annual generation numbers of most longicorn varieties undoubtedly increase the difficulty of artificial feeding. In particular, the difficulty of accurately and efficiently obtaining the polypide of different ages is increased.

However, more comprehensive studies on biological characteristics, developmental biology, supercooling points and freezing points of different insect states of brow-brow longicorn cattle require a large number of indoor populations with different insect states and different development degrees as test insect sources. At present, the simple and efficient indoor feeding technology and device are not reported at home and abroad. In view of this, it is important to research a raising method and a raising device for a brow spot and longhorn beetle in a matching manner in each insect state observation experiment, so as to ensure that the required day-old insect source is simply and efficiently obtained in related researches, and the experiment is smoothly carried out. The method and the device for raising the adeps gracilis in a matched manner in various insect state observation experiments are helpful for realizing the research needs in the aspects.

SUMMARY OF THE PATENT FOR INVENTION

The invention aims to provide a method and a device for feeding observable milbemyces texatilis and longhorn beetle larvae, and aims to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a method for breeding observable milbemyces eyebroworum larvae comprises the following steps:

(1) arranging a breeding room: selecting a feeding room according to the feeding scale, installing an exhaust fan in the feeding room to keep ventilation, and installing a cooling and heating air conditioning device to keep the experimental temperature;

(2) insect source collection: experimental population brothers and longicorn, selecting female worms which pass through the stage of laying eggs before mating, feeding fresh ceiba branches and male worms in an insect breeding bottle together, taking out the branches after 3-5 days, selecting effective laying egg notches at the stripped phloem, repeating the operation, replacing and collecting the ceiba branches with the laying egg notches once every three days, and then placing the ceiba branches in different larva feeding grids respectively;

(3) collecting feed: when the ceiba greening trees are pruned, the pruned fresh branches are collected, refrigerated and kept for standby feeding;

(4) and (3) low-age larvae: putting the egg-containing bark without taking eggs in the device for 5-8 days, hatching most of the eggs of the brow spot branchlet and longhorn beetle, entering 1-year larvae or taking insects for observation experiment, observing the notch and the periphery of the bark to form crumbs in 10-14 days, indicating that the vitality of the larvae is enhanced and entering 2-year larvae after entering 2-year, stripping the bark to remove the 2-year larvae, and facilitating the observation and breeding at the later time;

(5) old larvae: feeding larvae of 2 th instar into 3 th instar after molting on 15 th-20 th day, feeding larvae of 3 rd instar into 4 th instar after molting on 21 th-27 th day, and feeding food to help the larvae to grow;

(6) pupating: the pupal stage is started on day 32-38, the pupal stage is 12-15 days, no feed is added, and the observation experiment of pupal needs to be prepared in advance in the mature larva stage;

(7) adult: after eclosion, adults can wait for 5-6 days in a pupa chamber made of phloem residues in a larva feeding grid, and finally climb to the top end of the feeding grid due to the phototropism of the back of the adults, the adult is beaten before a strip-shaped drawer is drawn out due to the false death characteristic, the adults are directly caught in the feeding grid by tweezers after being pulled out, and after the adults come out of the tree, the adults are continuously fed in a successive transfer mode according to the steps (2) - (6).

Preferably, in the step (1), the area of the feeding chamber in the step (1) is 20-25m²And a stainless steel screen window is arranged to prevent the insects from escaping, and the indoor feeding environmental conditions are kept at the temperature of 25 +/-1 ℃ and the humidity of 75 +/-5%.

Preferably, in the step (2), two branches of the ceiba branches with the diameter of 1-2cm and one branch with the diameter of 3-5cm are selected, and the bark surrounding the oviposition notch is cut into the size of 2.0 x 2.0cm and taken out.

Preferably, in the step (2), when the bast is peeled off, the branch is firstly cut from one end against the xylem to the other end of the branch by using a blade, and the bast and the xylem are girdled and peeled off.

Preferably, in the step (4), a proper amount of phloem fragments or shreds should be periodically supplemented in the stage of the young larvae to cover the larva bodies so as to ensure sufficient food.

Preferably, in the step (5), the fed food is ensured to cover the insect body by more than 1cm, and a 1-2cm ceiba xylem strip is added.

Preferably, in the step (6), the preparation work needs to take out the larvae entering the prepupa and put the larvae into a 10-15mL medium-sized finger-type tube padded with toilet paper to be pupated.

The utility model provides a device is raised to observable eyebrow spot and spine longicorn larva, is including raising the box, it is provided with bar drawer and clearance steamer tray respectively to raise the inboard upper and lower place of box, the inside of bar drawer is provided with the same check of raising in a plurality of spaces, the inboard bottom of bar drawer is provided with the gauze, the front end fixedly connected with handle of bar drawer, the place ahead fixedly connected with handle of clearance steamer tray.

Preferably, the top of the feeding box is provided with a plurality of vent holes which are distributed in a matrix manner, and the center of the bottom of the inner side of the cleaning drawer is provided with a humidifying cup.

Preferably, the feeding box, the strip-shaped drawer and the cleaning drawer are all made of light-tight plastics, the aperture of the vent hole is 0.2cm, the number of the strip-shaped drawers is ten, the specifications of the strip-shaped drawers are 30 x 10cm, the specification of the feeding grid is 3.0 x 4.0cm, the specification of the cleaning drawer is 30 x 5cm, and the specification of the humidifying cup is 3.0 x 3.0 cm.

Compared with the prior art, the invention has the following beneficial effects:

1. the eyebrow spot and spine longicorn breeding device in each insect state is simple in structure, low in cost, easy to operate and recyclable, the device is integrally made of light-tight plastics except for the air holes and the gauze at the bottom of the breeding grid, a dark environment for survival of larva trunk borers is simulated, a plastic cup with a sieve mesh cover is arranged in the middle of the bottom layer food residue and insect feces collecting device and used for containing tap water, the structure is simple, the operation is convenient, and the internal humidity of the device is fully ensured to be proper;

2. the method breaks through the conventional method for raising the trunk borers of the longicorn larvae, is simple, solves the experimental problem that the biological characteristics, the developmental biology, the supercooling points and the freezing points of different insect states of the longicorn beetles with eyebrow spots and ridge horns need a large amount of indoor population requirements with different insect state development degrees, simply and efficiently provides insect sources for testing different ages and even different days, and ensures the smooth operation of related experiments;

3. according to the invention, the brow spot and longhorn beetle larva feeding box is used for uniformly accommodating the insect feeding grids, so that the space is saved, meanwhile, larva food residues and excrement are uniformly collected, the integral cleaning management is convenient, and the workload is saved;

4. according to the eyebrow spot and longhorn beetle larva breeding grid, the workload of independently cutting egg-containing grooving in a transfer method with a skin layer is reduced, the characteristic that the female insects select proper environment and space for the offspring at the initial stage of larva hatching is utilized, and under the condition that the hatchability of the eyebrow spot and longhorn beetle eggs is guaranteed, the situation that the hatching growth of the eyebrow spot and longhorn beetle is influenced by environmental change caused by human intervention is avoided, and the workload of artificial breeding in a laboratory is also reduced;

5. the method has the advantages that each worm egg laid by the female pregnant ova is separately fed, the characteristic that the larvae bite each other to kill the larvae is avoided, the survival rate of the larvae in the experimental population establishing process is greatly improved, the establishment of the experimental population is ensured at the highest speed, the quality guarantee and the efficiency increase are continuously realized for the population expansion, and a solid foundation is laid for the subsequent related experiments.

Drawings

FIG. 1 is a perspective view of a patented structure of the invention;

FIG. 2 is a sectional view of the structure of the strip drawer of the present invention;

fig. 3 is a schematic perspective view of a cleaning drawer according to the present invention.

In the figure: 1. feeding boxes; 2. a strip-shaped drawer; 3. cleaning the drawer; 4. breeding grids; 5. a screen; 6. a handle; 7. a handle; 8. a vent hole; 9. and (5) humidifying the cup.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by a person skilled in the art based on the embodiments in the patent of the invention without any inventive work belong to the protection scope of the patent of the invention.

A method for breeding observable milbemyces eyebroworum larvae comprises the following steps:

(1) arranging a breeding room: selecting a certain square breeding chamber according to breeding scale, installing an exhaust fan in the breeding chamber to keep ventilation, installing a cooling and heating air conditioning device to keep experiment temperature, wherein the breeding chamber is generally 20m²The stainless steel screen window is arranged to prevent the insects from escaping, the indoor feeding environmental conditions are kept at 25 +/-1 ℃ and 75 +/-5% of humidity;

(2) insect source collection: experimental population brothers and longicorn, selecting female worms which pass through the stage of laying eggs before mating, feeding fresh ceiba branches and male worms in an insect breeding bottle together, taking out branches after 3 days, selecting effective egg laying notches on a stripped phloem, repeating the operation, replacing and collecting ceiba branches with the egg laying notches once every three days, then respectively placing the ceiba branches in different larva feeding grids, selecting two branches of ceiba branches with the diameter of about 1.0cm and the diameter of one branch of 3.0-5.0cm, cutting the branches into barks with the size of 2.0 multiplied by 2.0cm around the egg laying notches, taking out the barks, when the phloem is stripped, firstly abutting the branches from one end to the other end of the branches by a blade, and girdling and stripping the phloem and the xylem;

(3) collecting feed: contacting related nursery gardens or related greening units, collecting and refrigerating trimmed fresh branches for standby feeding when the pruning units trim the kapok greening trees;

(4) and (3) low-age larvae: the method is characterized in that the egg-containing bark without taking eggs is placed in a device for about 5-8 days, most of the eggs of the brow-spotted and ridged cattle are hatched and enter 1-year larvae, 1-year larvae or the observation experiment requires holding time to take the larvae, the notch and the periphery of the bark can be seen to form crumbs in 10-14 days, so that the vitality of the larvae is enhanced, the larvae basically enter 2-year larvae, the bark is stripped to remove the 2-year larvae, the later observation and breeding are facilitated, and a proper amount of phloem fragments or broken strips are periodically supplemented in the stage of the young larvae to cover the bodies of the larvae, so that sufficient food is ensured;

(5) old larvae: feeding larvae of 3 instars after molting on day 15-20, feeding 3 instars after molting on day 21-27, feeding food to promote growth of larvae, feeding food to cover the larvae for more than 1cm, and feeding a piece of ceiba xylem strip of about 1 cm;

(6) pupating: the pupa stage is started in 32-38 days, the pupa stage is about 12d, no feed is added, the observation experiment of pupa needs to be prepared in advance in the mature larva stage, the preparation work needs to take out the larva which enters the prepupa, put the larva into a 10mL medium finger-shaped tube which is padded with toilet paper, and wait for the larva to pupate;

(7) adult: after eclosion, adults can wait for 5-6 days in a pupa chamber made of phloem residues in a larva feeding grid, and finally climb to the top end of the feeding grid due to the phototropism of the back of the adults, the adult is beaten before a strip-shaped drawer is drawn out due to the false death characteristic, the adults are directly caught in the feeding grid by tweezers after being pulled out, and after the adults come out of the tree, the adults are continuously fed in a successive transfer mode according to the steps (2) - (6).

Referring to fig. 1-3, a brow spot and spine longicorn larva observable breeding device comprises a breeding box 1, wherein a strip-shaped drawer 2 and a cleaning drawer 3 are respectively arranged above and below the inner side of the breeding box 1, a plurality of breeding grids 4 with the same space are arranged inside the strip-shaped drawer 2, a gauze 5 is arranged at the bottom of the inner side of the strip-shaped drawer 2, a handle 6 is fixedly connected to the front end of the strip-shaped drawer 2, a handle 7 is fixedly connected to the front end of the cleaning drawer 3, vent holes 8 are formed in the top of the breeding box 1, the number of the vent holes 8 is multiple and are distributed in a matrix manner, a humidifying cup 9 is arranged at the center of the bottom of the inner side of the cleaning drawer 3, the breeding box 1, the strip-shaped drawer 2 and the cleaning drawer 3 are made of light-tight plastics, the aperture of the vent holes 8 is 0.2cm, the number of the strip-shaped drawer 2 is ten, the specifications of the vent holes are 30 x 10cm, and the specifications of the breeding grids 4 are 3.0 x 4 x 4., the specification of the cleaning drawer 3 is 30 multiplied by 5cm, and the specification of the humidifying cup 9 is 3.0 multiplied by 3.0 cm.

The eyebrow spot and longhorn beetle scale culture method can breed 4-5 generations every year, and can breed all the year round under artificial temperature control, the eyebrow spot and longhorn beetle insect state observation and breeding device has the advantages of simple structure, low cost, simplicity, practicality and wide application range, and provides a large amount of insect sources for testing different insect state development degrees required by experiments for relevant scientific experiments such as the biological characteristics, the developmental biology, different insect state supercooling points, freezing points and the like of the eyebrow spot and the longhorn beetle.

Although embodiments of the present patent have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the present patent, the scope of which is defined in the appended claims and their equivalents.