阅读说明：本技术 一种调节血液葡萄糖水平的磁场发生装置及其应用 (Magnetic field generating device for adjusting blood glucose level and application thereof ) 是由 张欣 郁彪 田小飞 宋超 樊宜享 吕悦 于 2020-03-31 设计创作，主要内容包括：本发明公开一种调节血液葡萄糖水平的磁场发生装置,包括磁性体、笼子；所述笼子设置在所述磁性体上。具体可以通过将II型糖尿病小鼠置于笼子中,并一同放置在磁性体上。本发明还公开一种磁性体细胞处理装置以及上述磁性体细胞处理装置在调节离体肝癌细胞葡萄糖水平中的应用。本发明的调节血液葡萄糖水平的磁场发生装置的磁场场强分布具有持续、均匀、长期和稳定等特点,磁性体的S极可参与降低II糖尿病小鼠血糖。并通过对比不同磁场方向磁性体的使用,进一步改善调节血液葡萄糖水平的磁场发生装置的工作效率及利用率。本发明通过磁性体细胞处理装置在调节离体肝癌细胞葡萄糖水平中的应用,得知,磁性体的S极可参与调节肝癌细胞中葡萄糖的水平。(The invention discloses a magnetic field generating device for regulating blood glucose level, which comprises a magnetic body and a cage; the cage is disposed on the magnetic body. Specifically, type II diabetic mice are placed in a cage and placed together on a magnetic body. The invention also discloses a magnetic body cell processing device and application of the magnetic body cell processing device in regulating the glucose level of the isolated liver cancer cells. The magnetic field intensity distribution of the magnetic field generating device for regulating the blood glucose level has the characteristics of continuity, uniformity, long term, stability and the like, and the S pole of the magnetic body can participate in reducing the blood sugar of a II diabetic mouse. And the working efficiency and the utilization rate of the magnetic field generating device for regulating the blood glucose level are further improved by comparing the use of the magnetic bodies in different magnetic field directions. According to the application of the magnetic body cell processing device in regulating the glucose level of the in-vitro hepatoma cells, the S pole of the magnetic body can participate in regulating the glucose level of the hepatoma cells.)

1. Use of a magnetic body for regulating glucose level.

2. An application of magnetic body in regulating glucose of isolated hepatocarcinoma cell is provided.

3. The use of the magnetic body of claim 2 for modulating glucose in liver cancer cells ex vivo, comprising the steps of:

step one, constructing a HepG2-IR model:

step two, CCK-8 detects cell proliferation:

step three, detecting the glucose consumption of the HepG2-IR model: cells were placed on magnetic bodies under insulin stimulation and tested for glucose consumption in the HepG2-IR model.

4. The use of the magnetic body according to claim 3 for regulating glucose in isolated liver cancer cells, wherein the HepG2-IR model is constructed by inoculating 4000 cells/100 μ l per well into a 96-well culture plate to obtain a cell culture system, and dividing the cell culture system into a control group and a model group, wherein the concentration of palmitic acid in the cell culture system is 0.125-1 mmol/L after palmitic acid is added to the model group, and the cells in the control group and the model group are respectively placed in CO at 37 ℃₂Incubator (CO)₂The ratio of gas volume to total gas volume, v/v, was 5%) for 24h, yielding a HepG2-IR model.

5. Use of the magnetic body according to claim 3 or 4 for regulating glucose in isolated hepatoma cellsIn the third step, the magnetic substance has an S-pole facing the cell and is 4 × 10⁵The cells are paved on a 3.5cm cell culture dish at the density of each cell/ml, palmitic acid is added, the concentration of the palmitic acid in a cell culture system is 0.125 mmol/L, then the cells are placed on a magnetic body for culturing for 24 hours, insulin is added, the concentration of the insulin in the cell culture system is 100 nmol/L, the cells are stimulated for 20 minutes, and the cells are collected for detecting the glucose consumption.

6. A magnetic body cell processing device is characterized by comprising a magnetic body, a cell culture box and a cell culture dish; the magnetic body is placed in the cavity of the cell culture box, and the cell culture dish is arranged on the magnetic body and ensures that the S pole of the magnetic body is opposite to the cells in the cell culture dish.

7. The device for generating a horizontal magnetic field in isolated hepatoma cell glucose of claim 6, wherein said magnetic body is a neodymium iron boron permanent magnet.

8. A magnetic field generating device for regulating blood glucose level comprises a magnetic body, a cage; the cage is disposed on the magnetic body.

9. A magnetic field generating device for regulating blood glucose level comprises a magnetic body and a cover body; the cover body is covered on the outer side of the magnetic body, and the magnetic body is limited in the cover body.

10. The magnetic field generating device for regulating blood glucose level of claim 9, further comprising a screw; the magnetic body is fixed in the cavity of the cover body through the screw.

Technical Field

The invention relates to the technical field of glucose level regulation, in particular to a magnetic field generating device for regulating blood glucose level and application thereof.

Background

The magnetic field has a significant effect on the human body and other biological, water or chemical agents. At present, there are many techniques for making a magnetic field treatment apparatus and applying the magnetic field treatment apparatus to human body treatment by study.

A low frequency rotating permanent magnet high magnetic field therapeutic apparatus as disclosed in patent application 200820235822.8, wherein the guidance magnet is used to concentrate the magnetic force lines generated by the action magnet upwards, thereby reducing the leakage of the magnetic force lines and increasing the magnetic field intensity. Meanwhile, the upper and lower parts of the platform are respectively provided with the permanent magnets, so that magnetic lines of force generated by the upper and lower permanent magnets form a convection magnetic field, the magnetic field intensity and the height are further increased, and a better treatment effect is achieved.

Also, as disclosed in patent application 201820376376.6, a permanent magnet therapeutic device for medical use is disclosed, which is described in the patent application as being capable of treating bone fractures and osteoporosis by means of a steady and strong magnetic field generated by the permanent magnet assembly of the ring support.

The rotating magnetic field therapeutic instrument disclosed in patent application 201520598948.1 includes driving motor, cylindrical permanent magnet part and casing in its patent application, cylindrical permanent magnet part be the monoblock permanent magnet of radial magnetization from top to bottom, cylindrical permanent magnet part's cylinder axle center be provided with the pivot, both are integrated into one piece, driving motor fixes in the first end cover department of casing, pivot one end is connected with driving motor, the other end is connected with the shaft hole on the second end cover of casing. The novel rotary milling machine is simple in structure, reduces machining difficulty, is more stable in rotary operation, and can effectively prolong the service life.

Type II diabetes has become one of the leading causes of death worldwide and is one of the major problems to be solved urgently for maintaining human health. Therefore, how to develop and optimize a magnetic field generating device for regulating blood glucose level and expand its application, which helps to research the medical foundation of type II diabetes, clinical research and related drug development, is a problem that those skilled in the art need to solve at present.

Disclosure of Invention

The invention solves one of the technical problems by the following technical means: the prior art lacks a device for regulating blood glucose levels by the action of a magnetic field.

A magnetic field generator for regulating blood glucose level comprises a magnetic body, a cage; the cage is disposed on the magnetic body. Specifically, the type II diabetic mouse can be placed in a cage, and the cage with the mouse placed therein is placed on a magnetic body.

Preferably, the magnetic body is a neodymium iron boron permanent magnet but is not limited to the neodymium iron boron permanent magnet.

Preferably, the neodymium iron boron permanent magnet is composed of 12 blocks (length ×, width ×, height: 60mm × 50mm × 35mm) distributed in a matrix, has the characteristics of no power consumption, no heat generation, small occupied space and the like, and improves various physical properties of the device.

Preferably, there is the distance between the adjacent neodymium iron boron permanent magnet, and this distance can effectively improve the assembly nature, reduces the fault rate that inter attraction leads to between the neodymium iron boron permanent magnet to optimize structure and atress distribution promote each item physical property.

Preferably, the neodymium iron boron permanent magnet can be a neodymium iron boron permanent magnet, and also can be a plurality of neodymium iron boron permanent magnets which are spliced, such as mortise and tenon connection or threaded connection, or fastened through non-magnetic or weak magnetic screws to form the integral neodymium iron boron permanent magnet.

The magnetic field generating device for regulating blood glucose level of the present invention may also have the following structure:

a magnetic field generator for regulating blood glucose level comprises a magnetic body, a cover body; the cover body covers the outer side of the magnetic body, so that the magnetic body is limited in the cover body. Specifically, the magnetic body may be fixed in the cover body cavity by a screw.

Of course, other methods of the prior art, such as a method of directly placing the magnetic body in the cover, etc., which can realize the limit of the magnetic body in the cover, should also be within the protection scope of the present invention.

The cover of the present invention is preferably a non-magnetic or weakly magnetic stainless steel cover. The cover body is attractive, protects the magnetic body from being oxidized, avoids direct collision contact between a ferromagnetic object and the magnetic body, can also prevent the magnetic body from falling off in the moving process to generate accidents, further optimizes the structure and stress distribution and improves various physical properties.

The magnetic field intensity distribution of the magnetic field generating device for regulating the blood glucose level has the characteristics of continuity, uniformity, long term, stability and the like. And the working efficiency and the utilization rate of the magnetic field generating device for regulating the blood glucose level are further improved by comparing the use of the magnetic bodies in different magnetic field directions.

In order to verify the effect of the magnetic field generating device for regulating the blood glucose level, the device is used for regulating the blood sugar of a type II diabetes mouse and constructing a type II diabetes mouse model, so that the effect of regulating the blood glucose and effectively improving the liver cell steatosis can be realized.

The invention solves the second technical problem by the following technical means: the prior art lacks a cell processing device that regulates cellular glucose levels through the action of a magnetic field.

A magnetic cell processing device comprises a magnetic body, a cell culture box and a cell culture dish; the magnetic body is placed on the inner bottom surface of the cavity of the cell incubator, and preferably, the magnetic body is ensured to have an S pole upward, and an N pole downward is opposite to the inner bottom surface of the cavity of the cell incubator.

The cell culture dishes are arranged on the magnetic body from bottom to top and are limited in the cavity of the cell culture box. Each cell culture dish was loaded with cells.

Preferably, the cavity is divided by a partition board from bottom to top to form a 1 st layer, a 2 nd layer and a 3 rd layer. 3 of the cell culture dishes were placed in the corresponding layers.

Preferably, the magnetic body is a neodymium iron boron permanent magnet.

The cell incubator of the magnetic cell processing apparatus of the present invention may not include a partition plate, and a plurality of cell culture dishes may be directly stacked in the chamber of the cell incubator from the bottom to the top.

The NdFeB permanent magnet has the advantages that the S pole is upward, the maximum strength of the surface reaches 0.5T, and the length, the width and the height of the NdFeB permanent magnet are respectively 60mm, 50mm and 35 mm. The polarities of the upper and lower sides of the Nd-Fe-B permanent magnet are uniform, and the magnetic field gradient is maintained in a relatively small range.

Experiments have shown that the cell processing device of the present invention can be used to regulate cellular glucose levels.

The invention solves the third technical problem by the following technical means: the prior art lacks a method for regulating the glucose level of isolated hepatoma cells by the action of a magnetic field.

An application of the magnetic body cell processing device in regulating the glucose level of isolated hepatoma cells.

Preferably, the method comprises the following steps:

step one, constructing a HepG2-IR model:

step two, CCK-8 detects cell proliferation:

step three, detecting the glucose consumption of the HepG2-IR model: cells were placed on magnetic bodies under insulin stimulation and tested for glucose consumption in the HepG2-IR model.

Preferably, the HepG2-IR model is constructed by the specific steps of inoculating 4000 cells/100 mul per well to a 96-well culture plate to obtain a cell culture system, dividing the cell culture system into a control group and a model group, adding palmitic acid into the model group, wherein the concentration of the palmitic acid in the cell culture system is 0.125-1 mmol/L, and placing the cells of the control group and the model group in CO at 37 ℃ respectively₂Incubator (CO)₂The ratio of gas volume to total gas volume, v/v, was 5%) for 24h, yielding a HepG2-IR model.

Preferably, the magnetic body has an S-pole facing the cell according to 4 × 10⁵Cell density per cell/ml cells were plated on 3.5cm cell culture dishes and after addition of palmitic acid, the palmitic acid was on the cellsThe concentration of the cells in the culture system is 0.125 mmol/L, then the cells are placed on a magnetic body for culturing for 24h, insulin is added, after the insulin is added, the concentration of the insulin in the cell culture system is 100 nmol/L, the cells are stimulated for 20min, the cells are collected, and the glucose consumption is detected.

Preferably, the magnetic body is a neodymium iron boron permanent magnet.

Experiments show that the S pole of the magnetic body cell processing device can be involved in regulating the level of glucose in the hepatoma cells.

In summary, the advantages of the present invention are summarized as follows:

(1) the magnetic field generating device for regulating blood glucose level of the invention can regulate blood glucose level, and the S pole of the magnetic body can participate in reducing blood sugar of type II diabetic mice.

(2) The magnetic field intensity distribution of the magnetic field generating device for regulating the blood glucose level has the characteristics of continuity, uniformity, long term, stability and the like.

(3) By comparing the use of the magnetic bodies in different magnetic field directions, the working efficiency and the utilization rate of the magnetic field generating device for regulating the blood glucose level are further improved.

(4) Furthermore, the cover body is used in the magnetic field generating device for regulating the blood glucose level, so that the magnetic field generating device for regulating the blood glucose level forms a spatial magnetic field with more optimized distribution, the safety is improved, and the utilization rate of a strong magnetic field is increased.

(5) Furthermore, the application of the magnetic body cell processing device in the regulation of the glucose level of the in-vitro liver cancer cells shows that the S pole of the magnetic body can be involved in regulating the glucose level of the liver cancer cells.

Drawings

Fig. 1 is a perspective view of a magnetic field generating device for regulating blood glucose level based on a cover according to embodiment 1 of the present invention.

Fig. 2 is a schematic structural diagram of a magnetic field generating device for regulating blood glucose level based on a cover body in an orthographic view according to embodiment 1 of the invention.

Fig. 3 is a schematic structural diagram of a magnetic field generating device for regulating blood glucose level based on a cover body in a side view state according to embodiment 1 of the present invention.

Fig. 4 is a schematic structural diagram of a magnetic field generating device for regulating blood glucose level based on a cover body in a top view state according to embodiment 1 of the present invention.

Fig. 5 is a schematic structural view of a cage-based magnetic field generating device for regulating blood glucose level according to embodiment 1 of the present invention in an operating state.

FIG. 6 is a bar graph showing the relationship between the week age and the body weight of each group of mice in example 2 of the present invention.

FIG. 7 is a histogram showing the relationship between the week age and the change in blood glucose level of each group of mice in example 2 of the present invention.

FIG. 8 is a bar graph comparing food intake for groups of mice in example 2 of the present invention.

FIG. 9 bar graph comparing water intake for groups of mice in example 2 of the present invention.

FIG. 10 is a bar graph showing the time-dependent change of glucose level in blood after intraperitoneal injection of glucose into mice in each group in example 2 of the present invention.

FIG. 11 is a bar graph showing the time-dependent change in blood glucose levels after intraperitoneal injection of insulin into mice in each group in example 2 of the present invention.

FIG. 12 is a H & E stained section of liver tissue of each group of mice in example 2 of the present invention.

FIG. 13 is a H & E stained section of pancreatic tissues of each group of mice in example 2 of the present invention.

FIG. 14 is a H & E stained section of kidney tissue of each group of mice in example 2 of the present invention.

FIG. 15 is a H & E stained section of eyeball tissue of each group of mice in example 2 of the present invention.

FIG. 16 is a schematic structural diagram of an apparatus for constructing a model of type II diabetic mouse behavior in example 2 of the present invention.

FIG. 17 is a histogram of the number of times each group of mice entered the central area of the behavioural open field experimental apparatus in example 2 of the present invention.

FIG. 18 is a bar graph of the time taken for each group of mice in example 2 of the present invention to enter the central region of the behavioural open field experimental apparatus.

FIG. 19 is a histogram of the number of times each group of mice entered the peripherad area of the behavioural open field experimental apparatus of example 2 of the present invention.

FIG. 20 is a bar graph of the time taken for each group of mice in example 2 of the present invention to enter the peripherad area of the behavioural open field experimental apparatus.

FIG. 21 is a schematic view showing the structure of a magnetic cell processing apparatus including a partition plate according to example 2 of the present invention.

FIG. 22 is a schematic view showing the structure of a cell culture dish in example 2 of the present invention when the dish is stacked on a magnetic body.

FIG. 23 is a bar graph of the relationship between palmitic acid dosage and relative cell viability for example 3 of the present invention.

FIG. 24 is a bar graph showing the relationship between the amount of palmitic acid used and the amount of glucose consumed in example 3 of the present invention.

FIG. 25 is a bar graph comparing the glucose consumption of groups of cells of example 3 of the present invention under insulin stimulation.

In the animal and cell experiments, data were represented by mean ± standard deviation between the normal diet control group and the high fat diet control group, the high fat diet N-pole treated group, and the high fat diet S-pole treated group, ＊ represents p <0.05, ＊＊ represents p <0.01, "＊＊＊" represents p <0.001, between the high fat diet control group and the high fat diet N-pole treated group, and the high fat diet S-pole treated group, "#" represents p <0.05, "#" represents p <0.01, "# # represents p <0.001, in the cell experiments, between the blank control group and the palmitic acid treated group," ＊ "represents p <0.05," ＊＊ "represents p <0.01," ＊＊＊ "represents p <0.001, between the palmitic acid treated group and the palmitic acid combination group, and" represents p < 0.05.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.