阅读说明：本技术 一种荧光探测器、探测阵列以及制备荧光探测器的方法 (Fluorescence detector, detection array and method for manufacturing fluorescence detector ) 是由 陆众 张峰 龙跃金 韦琪 陆海龙 苏习明 于 2020-04-07 设计创作，主要内容包括：本发明涉及荧光探测技术领域,具体涉及一种荧光探测器、探测阵列以及制备荧光探测器的方法,该荧光探测器包括用于收集荧光的荧光收集器,其采用多根光纤制成,该荧光收集器包括采集端和探测端,荧光采集器从的采集端到探测端呈渐缩状,使得采集端的端面面积大于探测端的端面面积,采集端用于采集荧光信号,被采集的荧光信号在荧光探测器中传输时被汇集到探测端。再经过荧光探测电路探测将探测端汇集后的荧光信号转换成对应的电信号,本申请的荧光探测器具有灵敏度高、采集角度广以及响应时间短的优点。本申请提供的荧光探测阵列可以探测广角度和大范围内的荧光信号,并且可以采集到各个波长范围内的荧光信号。(The invention relates to the technical field of fluorescence detection, in particular to a fluorescence detector, a detection array and a method for preparing the fluorescence detector. The fluorescence signal after gathering with the detection end is surveyed through fluorescence detection circuit and is converted into corresponding signal of telecommunication again, and the fluorescence detector of this application has sensitivity height, the wide and short advantage of response time of collection angle. The fluorescence detection array provided by the application can detect fluorescence signals in a wide angle and a wide range, and can collect the fluorescence signals in various wavelength ranges.)

1. A fluorescence detector, characterized in that, the fluorescence detector comprises a fluorescence collector for collecting fluorescence, which is made of a plurality of optical fibers;

the fluorescence collector comprises a collecting end and a detecting end, the fluorescence collector is in a tapered shape from the collecting end to the detecting end, the area of the end face of the collecting end is larger than that of the end face of the detecting end, the collecting end is used for collecting fluorescence signals, and the collected fluorescence signals are collected to the detecting end when being transmitted in the fluorescence detector.

2. The fluorescence detector of claim 1, wherein said fluorescence detector is cone-shaped or truncated cone-shaped.

3. The fluorescence detector of claim 1, further comprising a filter disposed on an end face of said detection end for filtering interfering light signals received therein to allow fluorescence to pass therethrough.

4. The fluorescence detector of claim 3, further comprising a fluorescence detection circuit disposed at the detection end of the fluorescence collector for detecting the fluorescence signal collected by the detection end of the fluorescence collector and converting the fluorescence signal into a corresponding electrical signal.

5. The fluorescence detector of claim 4, wherein said fluorescence detection circuitry comprises a sensing element and acquisition circuitry electrically connected to the sensing element;

the sensing element is arranged at the detection end of the fluorescence collector and used for converting the fluorescence signal into an electric signal, and the acquisition circuit is used for acquiring the electric signal.

6. The fluorescence detector of claim 5, wherein said sensing element is positioned 0.5mm from the detection end of said fluorescence collector.

7. The fluorescence probe of claim 5, further comprising a sealed housing surrounding the fluorescence collector and sensing element.

8. A fluorescence detection array comprising a plurality of fluorescence detectors according to any of claims 1-7, the plurality of fluorescence detectors being closely arranged such that the collection ends of the fluorescence collectors of the plurality of fluorescence detectors are in the same plane and the sides of two adjacent fluorescence collectors are in close contact.

9. A fluorescence detection array comprising six fluorescence detectors according to any of claims 3 to 7, wherein the six fluorescence detectors are closely arranged such that the collection ends of the fluorescence collectors of the six fluorescence detectors are on the same plane and the sides of two adjacent fluorescence collectors are in close contact;

the filters with the wavelength ranges of 430 +/-30 nm, 490 +/-30 nm, 550 +/-30 nm, 610 +/-30 nm, 670 +/-30 nm and 730 +/-30 nm are respectively selected for the filters on the detection ends of the six fluorescence collectors.

10. A method for preparing a fluorescence detector according to any of claims 1-7, comprising: a plurality of optical fibers are sequentially subjected to regular arrangement, heating, pressure fusion, torsion and stretching to form a tapered fluorescence collector, so that the area of the end face of the collection end of the fluorescence collector is larger than that of the end face of the detection end of the fluorescence collector.

Technical Field

The invention relates to the technical field of fluorescence detection, in particular to a fluorescence detector, a detection array and a method for preparing the fluorescence detector.

Background

Fluorescence, a photoluminescence, is a property of luminescent materials. When a certain normal temperature substance is irradiated by incident light (usually ultraviolet rays or X rays) with a certain wavelength, the absorbed light energy enters an excited state, and immediately excites and emits emergent light with the wavelength of the incident light, which is called 'fluorescence', and the wavelength is usually in the visible light band, i.e. the wavelength range is 400-760 nm.

The application of fluorescence in our daily life is very wide, such as fluorescent lamps used for illumination, fluorescent pens used for marking, fluorescent markers in DNA detection and the like. Fluorescence detection is also one of the important detection methods in many fields, such as oil and gas exploration by using fluorescence analysis to identify whether a rock sample contains oil; in immunofluorescence examination, antibodies are fluorescently labeled and detected to determine the distribution positions of antigens, so that the fluorescence detection technology is a very practical and promising optical detection means, and has important influence on the research fields of biology, medicine, materials science and the like and the clinical medical diagnosis.

At present, two types of fluorescence detectors in the market mainly exist, one type is that a camera is adopted to detect fluorescence information with different wavelengths, the space range of the detection of the camera is large, but the sensitivity is low, and as the fluorescence signal is weak and the service life is short, the response process of the detection and the receiving of the camera is slow, and the acquisition time is long. The other method is a detection method adopting multi-fiber conduction, the response is faster, but the detection space range is smaller, namely the acquisition angle is small, the acquired intensity is small, and the requirement on the photoelectric detector is higher.

Disclosure of Invention

In order to solve the technical problems of small acquisition angle, long response time and low sensitivity of a fluorescence detector in the prior art, the application provides the following technical scheme.

A fluorescence detector, comprising a fluorescence collector for collecting fluorescence, made of a plurality of optical fibers;

the fluorescence collector comprises a collecting end and a detecting end, the fluorescence collector is in a tapered shape from the collecting end to the detecting end, the area of the end face of the collecting end is larger than that of the end face of the detecting end, the collecting end is used for collecting fluorescence signals, and the collected fluorescence signals are collected to the detecting end when being transmitted in the fluorescence detector.

Preferably, the fluorescence detector is conical or truncated cone-shaped.

In one embodiment, the fluorescence detector further comprises a filter disposed on the end face of the detection end for filtering the interference light signal used for passing through fluorescence.

In one embodiment, the fluorescence detector further comprises a fluorescence detection circuit disposed at the detection end of the fluorescence collector, and is configured to detect the fluorescence signal collected by the detection end of the fluorescence collector and convert the fluorescence signal into a corresponding electrical signal.

In one embodiment, the fluorescence detection circuit includes a sensing element and an acquisition circuit electrically connected to the sensing element;

the sensing element is arranged at the detection end of the fluorescence collector and used for converting the fluorescence signal into an electric signal, and the acquisition circuit is used for acquiring the electric signal.

In one embodiment, the sensing element is positioned 0.5mm from the detection end of the fluorescence collector.

In one embodiment, the fluorescent light collector further comprises a sealed housing surrounding the fluorescent light collector and the sensing element.

A fluorescence detection array comprises a plurality of fluorescence detectors as described above, wherein the fluorescence detectors are closely arranged, so that the collection ends of the fluorescence collectors of the fluorescence detectors are on the same plane, and the side faces of two adjacent fluorescence collectors are closely contacted.

A fluorescence detection array, comprising six fluorescence detectors as described above, wherein the six fluorescence detectors are closely arranged, so that the collection ends of the fluorescence collectors of the six fluorescence detectors are on the same plane, and the side faces of two adjacent fluorescence collectors are closely contacted;

the filters with the wavelength ranges of 430 +/-30 nm, 490 +/-30 nm, 550 +/-30 nm, 610 +/-30 nm, 670 +/-30 nm and 730 +/-30 nm are respectively selected for the filters on the detection ends of the six fluorescence collectors.

A method for preparing a fluorescence detector as described above, the method comprising: a plurality of optical fibers are sequentially subjected to regular arrangement, heating, pressure fusion, torsion and stretching to form a tapered fluorescence collector, so that the area of the end face of the collection end of the fluorescence collector is larger than that of the end face of the detection end of the fluorescence collector.

According to the fluorescence detector of the embodiment, the area of the collecting end of the fluorescence collector is large, the fluorescence signals in a large range and a large angle can be collected, the collected signals are transmitted through the optical fiber and collected at the detecting end, and the collected signals are detected by the fluorescence detection circuit to convert the fluorescence signals collected by the detecting end into corresponding electric signals. In addition, the fluorescence detection array of the embodiment can detect fluorescence signals in a wide angle and a wide range, can acquire the fluorescence signals in various wavelength ranges, and has the advantages of high acquisition precision and wide coverage range.

Drawings

FIG. 1 is a front view of a fluorescence collector according to an embodiment of the present application;

FIG. 2 is a top view of a fluorescent collector according to an embodiment of the present application;

FIG. 3 is a schematic structural diagram of a fluorescence detector according to an embodiment of the present application;

FIG. 4 is a top view of a fluorescence detection array according to an embodiment of the present application;

FIG. 5 is a front view of a fluorescence detection array according to an embodiment of the present application;

FIG. 6 is a rear view of a fluorescence detection array according to an embodiment of the present application.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The fluorescence detector provided by the embodiment of the invention comprises the fluorescence collector for collecting fluorescence, and the fluorescence collector is made of a plurality of optical fibers and has the advantages of high transmission speed, high sensitivity and small signal loss during transmission. This its fluorescence collector's collection end area is great, can gather on a large scale and the wide-angle fluorescence signal, and the signal that passes through of gathering collects at the detection end through optical fiber transmission and collection, surveys through fluorescence detection circuit again and convert the fluorescence signal that the detection end collected into corresponding signal of telecommunication, and the fluorescence detector of this embodiment has sensitivity height, gathers the advantage that the angle is wide and response time period with prior art's comparison. In addition, the fluorescence detection array comprises a plurality of fluorescence detectors, can detect fluorescence signals in a wide angle and a wide range, can acquire the fluorescence signals in various wavelength ranges, and has the advantages of high acquisition precision and wide coverage range.