阅读说明：本技术 一种上转化荧光检测装置与检测方法 (Up-conversion fluorescence detection device and detection method ) 是由 雍金贵 缪连军 张磊 于 2020-04-09 设计创作，主要内容包括：本发明公开了一种上转化荧光检测装置与检测方法,该检测装置包括传送装置,驱动传送装置的动力装置,设置在传送装置侧边的激发装置与摄像装置,以及设置在传送装置一端的矫正装置与整平装置,矫正装置用于辅助工作人员对传送装置上载体的位置进行矫正,保证后续检测的准确性,所述激发装置包括透光圈固定板与激发光源,以LED灯珠作为激发光源,将透光圈设置在激发光源出光点的正下方,通过透光圈固定板的设置,限制激发光源的照射范围,降低反射、漫射等因素对荧光反应激发的影响保证检测结果的准确性,综上所述,本发明所述上转化荧光检测装置能够连续进行荧光检测,并具有较高的检测一致性与准确性。(The invention discloses an up-conversion fluorescence detection device and a detection method, wherein the detection device comprises a conveying device, a power device for driving the conveying device, an excitation device and a camera device which are arranged on the side edge of the conveying device, and a correction device and a leveling device which are arranged at one end of the conveying device, wherein the correction device is used for assisting a worker to correct the position of a carrier on the conveying device and ensuring the accuracy of subsequent detection.)

1. The up-conversion fluorescence detection device is characterized by comprising a conveying device (1), wherein the conveying device (1) is driven by a power device (2), an excitation device (3) and a camera device (4) are arranged on the side edge of the conveying device (1), and a correction device (5) and a leveling device (6) are arranged at one end of the conveying device (1);

the conveying device (1) comprises a base platform (11), a light transmission groove (12) is formed in the middle of the base platform (11), a camera light transmission groove (13) is formed in the middle of the base platform (11), roller shafts (14) are rotatably arranged at two ends of the base platform (11), two ends of each roller shaft (14) are rotatably connected with the base platform (11) through bearings, and the two roller shafts (14) are connected through a conveying belt;

the excitation device (3) comprises a sliding main rod (31), a convex sliding rail is arranged on one side of the sliding main rod (31), the sliding main rod (31) is connected with two sliding blocks in a sliding mode through the sliding rail, one sliding block is fixedly connected with a light source fixing plate (33), the other sliding block is fixedly connected with a light-transmitting ring fixing plate (34), an excitation light source (35) is fixedly installed on the light source fixing plate (33), and a circular light-transmitting ring (36) penetrating through the light-transmitting ring fixing plate (34) is arranged on the light-transmitting ring fixing plate (34); the light-transmitting diaphragm (36) is arranged right below the light-emitting point of the excitation light source (35).

2. The up-conversion fluorescence detection device according to claim 1, wherein the power device (2) comprises a driving motor (21), a shaft extension end of the driving motor (21) is connected with a transmission shaft, a motor housing of the driving motor (21) is fixedly connected with a mounting frame (23) through a plurality of stabilizer bars (22), the mounting frame (23) is fixedly mounted on the frame, a gear is fixedly sleeved on the transmission shaft, gears are respectively sleeved at two ends of the roller shaft (14), and the gear on the roller shaft (14) is connected with the gear sleeved on the transmission shaft through a belt or a chain.

3. The up-conversion fluorescence detection device according to claim 1, wherein the correction device (5) comprises a main rod (51), two ends of the main rod (51) are rotatably connected with a threaded rod (52), a threaded sleeve (53) is sleeved on the threaded rod (52), the threaded sleeve (53) is in threaded fit with the threaded rod (52), the threaded sleeve (53) is further sleeved on the main rod (51), the threaded sleeve (53) is in sliding fit with the main rod (51), a mounting rod (54) is further fixedly mounted on the threaded sleeve (53), a mounting plate (55) is fixedly mounted on the mounting rod (54), a laser lamp bead (56) is fixedly mounted on the mounting plate (55), and a laser ray of the laser lamp bead (56) is projected to one point on the surface of the conveyor belt.

4. The up-conversion fluorescence detection device according to claim 1, wherein the leveling device (6) comprises a leveling main rod (61), an adjusting mounting plate (62) is fixedly mounted at the end of the leveling main rod (61) through a bolt, a strip-shaped hole is formed in the adjusting mounting plate (62), a mounting block (63) is fixedly mounted on the adjusting mounting plate (62) through a bolt, and the mounting block (63) is rotatably connected with one end of a pressing shaft (64).

5. The up-conversion fluorescence detection device according to claim 1, wherein the excitation light source (35) is a multi-spectral L ED light source.

6. The up-conversion fluorescence detection device according to claim 1, wherein the camera device (4) is an industrial camera.

7. The up-conversion fluorescence detection device according to claim 1, wherein the up-conversion fluorescence detection method by the up-conversion fluorescence detection device is:

s1, reacting the up-conversion fluorescent molecular probe with an object to be detected on a carrier, wherein the up-conversion fluorescent molecular probe is a material which is excited by high-energy light and emits low-energy light;

s2, fixing the reacted carrier on a conveyor belt, driving the conveyor belt to rotate by a power device (2), transmitting the carrier to the lower part of an excitation light source (35), starting the excitation light source to irradiate the carrier for a preset time T, then transmitting the carrier to the vicinity of an image pickup device (4), and shooting by the image pickup device (4) to obtain a strip generated by the fluorescence reaction on the corresponding carrier.

Technical Field

The invention belongs to the technical field of fluorescence detection, and particularly relates to an up-conversion fluorescence detection device and a detection method.

Background

The upper conversion luminescence is derived from Stokes' law, which considers that the material is intelligently excited by high-energy light and emits low-energy light, and the application of the upper conversion technology to the actual detection is to use the converted fluorescent material as a raw material to manufacture corresponding detection test paper and detect according to the concentration of a reactant generated by a test target and the test paper;

Disclosure of Invention

The invention aims to provide an up-conversion fluorescence detection device and a detection method.

The technical problems to be solved by the invention are as follows:

Drawings

The invention is described in further detail below with reference to the figures and specific embodiments.

FIG. 1 is a schematic structural diagram of an up-conversion fluorescence detection device according to the present invention;

FIG. 2 is a schematic diagram of the excitation device;

FIG. 3 is a schematic view of the orthotic device;

FIG. 4 is a schematic structural view of a transfer device;

FIG. 5 is a schematic structural diagram of a power plant;

figure 6 is a schematic view of the structure of the flattening apparatus.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

An up-conversion fluorescence detection device, as shown in fig. 1 to 6, comprises a conveying device 1, wherein the conveying device 1 is driven by a power device 2, an excitation device 3 and an image pickup device 4 are arranged at the side edge of the conveying device 1, and a correction device 5 and a leveling device 6 are arranged at one end of the conveying device 1;

the conveying device 1 comprises a base platform 11, a light transmission groove 12 is arranged in the middle of the base platform 11, a camera light transmission groove 13 is arranged in the middle of the base platform 11, roller shafts 14 are rotatably arranged at two ends of the base platform 11, two ends of each roller shaft 14 are rotatably connected with the base platform 11 through bearings, and the two roller shafts 14 are connected through a conveying belt;

the power device 2 comprises a driving motor 21, a shaft extension end of the driving motor 21 is connected with a transmission shaft, a motor shell of the driving motor 21 is fixedly connected with an installation frame 23 through a plurality of stabilizer bars 22, the installation frame 23 is fixedly installed on a rack, gears are fixedly sleeved on the transmission shaft, gears are respectively sleeved at two ends of the roll shaft 14, and the gears on the roll shaft 14 are connected with the gears sleeved on the transmission shaft through belts or chains;

the correcting device 5 comprises a main rod 51, wherein two ends of the main rod 51 are rotatably connected with a threaded rod 52, a threaded sleeve 53 is sleeved on the threaded rod 52, the threaded sleeve 53 is in threaded fit with the threaded rod 52, the threaded sleeve 53 is further sleeved on the main rod 51, the threaded sleeve 53 is in sliding fit with the main rod 51, a mounting rod 54 is further fixedly mounted on the threaded sleeve 53, a mounting plate 55 is fixedly mounted on the mounting rod 54, a laser lamp bead 56 is fixedly mounted on the mounting plate 55, a laser ray is emitted through the correcting device 5, the laser ray is projected to a certain point of a conveyor belt, the position of the carrier is adjusted through the position of the laser spot, the correcting device is used for assisting a worker to correct the position of the carrier on the conveying device, and the strip generated by fluorescence reaction on the carrier is in the preset position in a photo in the subsequent detection process, so that the accuracy of a detection result is,

the leveling device 6 comprises a leveling main rod 61, an adjusting mounting plate 62 is fixedly mounted at the end part of the leveling main rod 61 through a bolt, a strip-shaped hole is formed in the adjusting mounting plate 62, a mounting block 63 is fixedly mounted on the adjusting mounting plate 62 through a bolt, the mounting block 63 is rotatably connected with one end of a pressing shaft 64, the pressing shaft 64 is used for extruding and leveling the carrier on the conveying belt, and the paper carrier is easy to deform in the conveying and wetting and drying processes, so that strips generated by fluorescence reaction on the carrier collected by a camera device are deformed in the subsequent detection process, the comparative analysis is difficult, the accuracy of the detection result is influenced, and the carrier passing through the lower part of the leveling device can be flattened through the leveling device;

the excitation device 3 comprises a sliding main rod 31, a convex sliding rail is arranged on one side of the sliding main rod 31, the sliding main rod 31 is connected with two sliding blocks in a sliding manner through the sliding rail, one sliding block is fixedly connected with a light source fixing plate 33, the other sliding block is fixedly connected with a light-transmitting ring fixing plate 34, an excitation light source 35 is fixedly arranged on the light source fixing plate 33, and a circular light-transmitting ring 36 penetrating through the light-transmitting ring fixing plate 34 is arranged on the light-transmitting ring fixing plate 34; the light-transmitting ring 36 is arranged right below the light-emitting point of the excitation light source 35, and the irradiation range of the excitation light source 35 is limited by the arrangement of the light-transmitting ring fixing plate 34, so that the influence of reflection, diffusion and other factors on the excitation of the fluorescence reaction is reduced;

the excitation light source 35 is a multi-spectral L ED light source;

the camera device 4 is an industrial camera and is used for shooting the carrier passing through the camera device 4 to obtain a strip generated by a fluorescence reaction on the corresponding carrier and transmitting the shot picture to a corresponding analysis module for analysis and detection;

the method for detecting the up-conversion fluorescence by the up-conversion fluorescence detection device comprises the following steps:

s1, reacting the up-conversion fluorescent molecular probe with an object to be detected on a carrier, wherein the up-conversion fluorescent molecular probe is a material which is excited by high-energy light and emits low-energy light;

and S2, fixing the reacted carrier on a conveyor belt, driving the conveyor belt to rotate by the power device 2, transmitting the carrier to the position below the excitation light source 35, starting the excitation light source to irradiate the carrier for a preset time T, transmitting the carrier to the position near the camera device 4, and shooting by the camera device 4 to obtain a strip generated by the fluorescence reaction on the corresponding carrier.

The up-conversion fluorescence detection device can continuously perform fluorescence detection and has higher detection consistency and accuracy.

The foregoing is merely exemplary and illustrative of the present invention and various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the scope of the invention as defined in the following claims.