阅读说明：本技术 一种修复高含油黏土的组合物及其修复方法 (Composition for repairing high-oil-content clay and repairing method thereof ) 是由 李金灵 李小康 屈撑囤 鱼涛 杨博 陈刚 朱世东 于 2020-04-02 设计创作，主要内容包括：本发明涉及一种修复高含油黏土的组合物及其修复方法。所述组合物包括牛粪5～10份、沙土40～50份、植物碎屑5～10份、亚甲基尿素0.5～1份、卵磷脂1～1.5份、Tween80 0.3～0.5份、复合微生物菌剂5～8份,通过向改性土壤中添加不动杆菌、无色杆菌和铜绿假单胞菌组成的复合微生物菌剂,质量比为5:1:5。所述组合物能降低土壤含油量和土壤粘度,增强黏土颗粒中的石油转移到牛粪和植物碎屑上,使微生物能很好地生存并修复改性后的土壤,加强土壤后期的生态恢复。(The invention relates to a composition for repairing clay with high oil content and a repairing method thereof. The composition comprises 5-10 parts of cow dung, 40-50 parts of sandy soil, 5-10 parts of plant debris, 0.5-1 part of methylene urea, 1-1.5 parts of lecithin, 800.3-0.5 part of Tween and 5-8 parts of a composite microbial agent, wherein the composite microbial agent consisting of acinetobacter, achromobacter and pseudomonas aeruginosa is added into modified soil in a mass ratio of 5:1: 5. The composition can reduce the oil content and the viscosity of soil, and can enhance the transfer of petroleum in clay particles to cow dung and plant debris, so that microorganisms can well survive and repair the modified soil, and the later ecological restoration of the soil is enhanced.)

1. the composition for repairing the high-oil clay is characterized by comprising the following raw materials in parts by weight: 5-10 parts of cow dung, 40-50 parts of sandy soil, 5-10 parts of plant debris, 0.5-1 part of methylene urea, 1-1.5 parts of lecithin, 800.3-0.5 part of Tween and 5-8 parts of a compound microbial agent.

2. The composition of claim 1, wherein: the compound microbial agent consists of acinetobacter, pseudomonas aeruginosa and achromobacter.

3. The composition of claim 2, wherein: the mass ratio of the acinetobacter to the achromobacter to the pseudomonas aeruginosa is 5:1: 5.

4. The composition according to claim 1, characterized by comprising the following raw materials in parts by weight: 5-10 parts of cow dung, 40-50 parts of sandy soil, 5-10 parts of plant debris, 0.5-1 part of methylene urea, 1-1.5 parts of lecithin, 800.3-0.5 part of Tween and 5-8 parts of a compound microbial agent.

5. A method for repairing high oil-containing clay is characterized by comprising the following steps: use of a composition according to any one of claims 1 to 4.

6. The method according to claim 5, characterized by comprising the steps of:

(1) drying, crushing and sieving the dried cow dung after composting to prepare dung residues;

(2) sieving sandy soil;

(3) air drying plant leaves, and crushing;

(4) uniformly mixing high oil-containing clay and sandy soil, adding the excrement residue and the plant debris, uniformly mixing, adding Tween80 and lecithin, and uniformly mixing again;

(5) adding methylene urea;

(6) the pseudomonas aeruginosa, the achromobacter and the acinetobacter are respectively subjected to enrichment culture and centrifugation and then prepared into OD by using physiological saline₆₀₀0.8-1.2 of bacterial suspension, and according to the volume of the bacterial suspension of 5:1:5 mixing and preparing the mixture into a mixed microbial inoculum for later use.

(7) And (5) adding the composite microbial inoculum into the soil in the step (5), and uniformly mixing.

7. The method according to claim 6, characterized by comprising the steps of:

(1) the content indexes of the used dried cow dung after composting are as follows: the total organic matter content (dry basis) is 140g/Kg, the organic matter content (dry basis) is dried until the water content is 1-5%, and the organic matter content is crushed and sieved by a 5-10 mesh sieve to prepare excrement residues;

(2) the content index of the sandy soil is 32g/Kg of total organic matter content (dry basis), plant residues are picked off and sieved by a sieve with 25-30 meshes;

(3) drying plant leaves by air, and crushing the plant leaves to a particle size of 1-2 mm by using a crusher;

(4) uniformly mixing 40-50 parts of high-oil-content clay and 40-50 parts of sandy soil, adding 5-10 parts of cow dung and 5-10 parts of plant debris, uniformly mixing again, adding Tween80 and lecithin, uniformly mixing again to make the two surfactants fully contact with petroleum hydrocarbon in soil, emulsifying and eluting the petroleum hydrocarbon in the soil, wherein the dry cow dung can well adsorb the petroleum hydrocarbon in the soil;

(5) adding 0.5-1 part of methylene urea;

(6) and adding 5-8 parts of the constructed compound microbial agent into the modified soil, and mixing again.

8. Use of a composition according to any one of claims 1 to 4 for environmental protection, preferably for the remediation of high oil clays.

Technical Field

The invention belongs to the technical field of high oil content viscous soil treatment and soil remediation, and particularly relates to a modification and microbial remediation method for high oil content viscous soil.

Background

During mining, transportation and refining, large quantities of oil can leak and cause persistent environmental damage. Nearly 30 million tons of oil are mined worldwide each year, of which 800 million tons will flow into the environment to produce as much as 10 million tons of contaminated soil and sludge. Petroleum is a hydrophobic substance which is easy to combine with soil and reduces the porosity of the soil, and particularly high oil-bearing clay combines with water to form a cohesive soil block, so that the conventional remediation technology cannot effectively remediate the polluted soil. The petroleum compounds at this time prevent the absorption of nutrients by the roots of plants, thereby causing various plants to die and further aggravating the degree of contamination, resulting in pollution that is hardly naturally degraded. Therefore, there is a need for effective remediation methods to treat those contaminated soils. From the social concept of energy conservation, environmental protection, high efficiency and no secondary pollution, the microbial remediation technology is considered as a promising method for remedying the oil-contaminated soil. In addition, microbial remediation can greatly improve soil fertility and help to restore ecological diversity of soil. Although petroleum-degrading bacteria are available in various parts of the nature, microbial activity is still low due to nutritional and environmental limitations. As a method for changing the microbial degradation activity in soil, biostimulation can satisfy the metabolic requirements of microorganisms and enhance the microbial degradation activity by adding nutrients to soil. Recently, many studies have been reported to improve the efficiency of microbial degradation of petroleum hydrocarbons in soil by using stimulation techniques. The results indicate that biostimulation can effectively increase the number of microorganisms in the soil and the efficiency of degrading petroleum compounds. The inventor of the application reduces the oil content and viscosity of the soil by adding cow dung, sandy soil, plant debris, Tween80, lecithin and methylene urea in a certain proportion, so that the oil-containing clay is more loose and rich in nutrition, a proper environment and power are provided for later-stage microbial remediation, experiments show that the remediation effect is obviously improved, and the invention is further completed.

Disclosure of Invention

The invention provides a composition for repairing clay with high oil content.

Another objective of the present invention is to provide a method for repairing clay with high oil content.

The invention also aims to provide the application of the composition for repairing the clay with high oil content.

In order to realize the purpose, the invention adopts the following technical scheme:

aiming at the problems in the prior art, the invention aims to provide a soil conditioner which can obviously improve the soil structure, has the effects of water and fertilizer retention, promotes the nutrient absorption of plants, reduces the absorption of heavy metals and improves the crop quality, and a preparation method thereof.

The technical scheme is as follows:

the composition for repairing the high-oil clay comprises the following raw materials in parts by weight: 5-10 parts of cow dung, 40-50 parts of sandy soil, 5-10 parts of plant debris, 0.5-1 part of methylene urea, 1-1.5 parts of lecithin, 800.3-0.5 part of Tween and 5-8 parts of a compound microbial agent.

The compound microbial agent consists of acinetobacter, pseudomonas aeruginosa and achromobacter.

The mass ratio of the acinetobacter to the achromobacter to the pseudomonas aeruginosa is 5:1: 5.

Preferably, 5-10 parts of cow dung, 40-50 parts of sandy soil, 5-10 parts of plant debris, 0.5-1 part of methylene urea, 1-1.5 parts of lecithin, 800.3-0.5 part of Tween and 5-8 parts of a compound microbial agent.

A method for repairing high oil-bearing clay, which uses the composition.

Preferably, the method for repairing the high oil-bearing clay comprises the following steps:

(1) drying, crushing and sieving the dried cow dung after composting to prepare dung residues;

(2) sieving sandy soil;

(3) air drying plant leaves, and crushing;

(4) uniformly mixing high oil-containing clay and sandy soil, adding the excrement residue and the plant debris, uniformly mixing, adding Tween80 and lecithin, and uniformly mixing again;

(5) adding methylene urea;

(6) and (5) adding the composite microbial inoculum into the soil in the step (5), and uniformly mixing.

7. The method according to claim 6, characterized by comprising the steps of:

(1) the content indexes of the used dried cow dung after composting are as follows: the total organic matter content (dry basis) is 140g/Kg, the organic matter content (dry basis) is dried until the water content is 1-5%, and the organic matter content is crushed and sieved by a 5-10 mesh sieve to prepare excrement residues;

(2) the content index of the sandy soil is 32g/Kg of total organic matter content (dry basis), plant residues are picked off and sieved by a sieve with 25-30 meshes;

(3) drying plant leaves by air, and crushing the plant leaves to a particle size of 1-2 mm by using a crusher;

(4) uniformly mixing 40-50 parts of high-oil-content clay and 40-50 parts of sandy soil, adding 5-10 parts of cow dung and 5-10 parts of plant debris, uniformly mixing again, adding Tween80 and lecithin, uniformly mixing again to make the two surfactants fully contact with petroleum hydrocarbon in soil, emulsifying and eluting the petroleum hydrocarbon in the soil, wherein the dry cow dung can well adsorb the petroleum hydrocarbon in the soil;

(5) adding 0.5-1 part of methylene urea;

(6) and adding 5-8 parts of the constructed compound microbial agent into the modified soil, and mixing again.

The method can be used for environmental protection, and is preferably used for repairing high-oil clay.

Further, the repair and preparation method comprises the following steps:

(1) selecting the cow dung which is composted in the cattle farm in Xinjiang, wherein the content indexes are as follows: the total organic matter content (dry basis) is 140g/Kg, the organic matter content (dry basis) is dried until the water content is 1-5%, and the organic matter content is crushed and sieved by a 5-10 mesh sieve to prepare excrement residues;

(2) the sandy soil is Xinjiang Gobi sandy soil, the content index of the Xinjiang Gobi sandy soil is 32g/Kg of total organic matter content (dry basis), plant residues are picked off, and the Xinjiang Gobi sandy soil is sieved by a 25-30-mesh sieve;

(3) the plant debris is made of piled and rotten poplar leaves, and is air-dried and crushed to the particle size of 1-2 mm by using a crusher;

(4) uniformly mixing 40-50 parts of high-oil-content clay and 40-50 parts of sandy soil, adding 5-10 parts of cow dung and 5-10 parts of plant debris, uniformly mixing again, adding Tween80 and lecithin, uniformly mixing again to make the two surfactants fully contact with petroleum hydrocarbon in soil, emulsifying and eluting the petroleum hydrocarbon in the soil, and enabling the dry cow dung to well adsorb the petroleum hydrocarbon in the soil.

(5) And adding a nutrient solution containing 0.5-1 part of methylene urea to further improve the soil nutrient environment.

(6) At the moment, 5-8 parts of the constructed composite microbial inoculum is added into the modified soil, the mixture is mixed again, and the water content of the soil is measured and kept at 25%.

The invention belongs to the technical field of high oil content viscous soil treatment and soil remediation, and particularly relates to a method for microbial remediation of high oil content viscous soil. Wherein, the dry cow dung and the fine sand of the compost are added into the high-oil-content clay to well reduce the soil viscosity and the oil content, and then the added plant debris can help the soil to stabilize the porosity, provide a proper environment for the survival of the microbial inoculum and stimulate the microorganisms to further improve the degradation efficiency of petroleum. The addition of the methylene urea improves the content of a nitrogen source deficient in the oil-containing clay, the soil nutrition is further balanced by adding other nutrient solutions, the added nutrient substances can be absorbed by the dried cow dung, the moisture and fertility preserving capability of the soil is improved, the nutrition loss is reduced, the cow dung at the moment can adsorb petroleum hydrocarbon in the soil with the help of the surfactant, and the petroleum pollution can be better repaired by microorganisms.

The invention has the beneficial effects that:

(1) the invention aims at the modification method of high oil-containing clay (the oil content is less than or equal to 9 percent), the raw materials are easy to obtain, the operation method is simple and effective, the cost is low, and the method is environment-friendly and pollution-free;

(2) the experiment adopts a soil modification method, which can enhance the transfer of petroleum hydrocarbon in soil, improve the removal of petroleum pollutants in clay by microorganisms and contribute to the ecological restoration of soil in later period.

(3) The compound microbial inoculum has no antagonistic action among strains, and can effectively degrade most of hydrocarbon substances in soil.

Drawings

FIG. 1 is a standard curve of an infrared oil gauge;

FIG. 2 is a strain growth curve;

FIG. 3 is a GC-MS spectrum of a biocide petroleum hydrocarbon;

FIG. 4 is a GC-MS spectrum of the mixed bacterial agent on petroleum hydrocarbon degradation.

Examples

The invention is further illustrated by the following examples. It should be understood that the method described in the examples is only for illustrating the present invention and not for limiting the present invention, and that simple modifications of the preparation method of the present invention based on the concept of the present invention are within the scope of the claimed invention. The starting materials and solvents used in the examples are all commercially available products.

Petroleum-degrading bacteria separated from petroleum-contaminated soil were cultured in sterile LB medium on a constant temperature incubator shaker (180rpm, 35 ℃, 24h, model 80501). The bacteria were purified by repeated centrifugation of the solution (9000rpm, 4 ℃, 15 minutes, centrifuge model TG20G), decantation of the supernatant and resuspension in sterile saline solution (0.85%) three times. Measuring absorbance (OD) at 600 of wavelength with ultraviolet spectrophotometer₆₀₀) The OD is prepared by respectively carrying out enrichment culture and centrifugation on pseudomonas aeruginosa, achromobacter and acinetobacter and then using physiological saline₆₀₀0.8-1.2 of bacterial suspension, and according to the volume of the bacterial suspension of 5:1:5 mixing to prepare a mixed bacterial agent for standby, and finally adding the bacterial liquid into the soil of each experimental group.

As shown in FIG. 2, the growth amounts of several single strains in the culture medium show S-type growth, and the growth curves of the mixed strains (Acinetobacter, Achromobacter and Pseudomonas aeruginosa) also show S-type growth, which indicates that no antagonism exists among the mixed strains at this time, and a higher bacterial growth amount is shown. By comparing the degradation effects of fig. 3 and fig. 4, the degradation effect of the mixed bacteria is obvious, and three strains of bacteria can be used as the mixed bacteria to degrade petroleum pollutants. The three strains of bacteria show high biological activity no matter under independent or mixed conditions, and can be used as remediation microbial inoculum for treating petroleum pollution.

The soil component ratios are shown in the following table: