阅读说明：本技术 一种mri兼容的离体肾脏灌注系统 (MRI (magnetic resonance imaging) -compatible in-vitro kidney perfusion system ) 是由 张嘉 于 2019-12-23 设计创作，主要内容包括：本发明提供一种MRI兼容的离体肾脏灌注系统。所述MRI兼容的离体肾脏灌注系统包括：放置皿；放置槽,所述放置槽开设在放置皿的底部内壁上；离体肾脏,所述离体肾脏放置在放置槽中；环形储液腔,所述环形储液腔开设在放置皿上；储液进口管,所述储液进口管固定安装在放置皿的一侧,所述储液进口管与环形储液腔相连通；储液出口管,所述储液出口管固定安装在放置皿的一侧,所述储液出口管与环形储液腔相连通；脉冲气源发生器。本发明提供的MRI兼容的离体肾脏灌注系统具有对离体肾脏进行成像扫描且不会造成创伤、在对肾脏进行脉冲的同时不会对肾脏造成感染,在对肾脏进行存取时更为方便的优点。(The invention provides an MRI compatible ex vivo kidney perfusion system. The MRI compatible ex vivo renal perfusion system comprises: placing a dish; the placing groove is formed in the inner wall of the bottom of the placing vessel; an ex vivo kidney placed in a holding tank; the annular liquid storage cavity is formed in the placing vessel; the liquid storage inlet pipe is fixedly arranged on one side of the placing vessel and communicated with the annular liquid storage cavity; the liquid storage outlet pipe is fixedly arranged on one side of the placing vessel and communicated with the annular liquid storage cavity; a pulse air source generator. The MRI compatible in-vitro kidney perfusion system provided by the invention has the advantages that the in-vitro kidney is subjected to imaging scanning, no wound is caused, the kidney is not infected while the kidney is pulsed, and the kidney is more convenient to access.)

1. An MRI compatible ex vivo renal perfusion system, comprising:

placing a dish;

the placing groove is formed in the inner wall of the bottom of the placing vessel;

an ex vivo kidney placed in a holding tank;

the annular liquid storage cavity is formed in the placing vessel;

the liquid storage inlet pipe is fixedly arranged on one side of the placing vessel and communicated with the annular liquid storage cavity;

the liquid storage outlet pipe is fixedly arranged on one side of the placing vessel and communicated with the annular liquid storage cavity;

the pulse air source generator is fixedly arranged on one side of the placing vessel;

a valve body contained within a pulsed air source generator;

the air cavity is arranged in the valve body;

the exhaust hole is formed in the inner wall of one side of the air cavity;

the silicone tube is fixedly arranged in the air cavity, and the top end and the bottom end of the silicone tube extend out of the air cavity;

the two one-way valves are fixedly arranged at the top end and the bottom end of the silicone tube respectively;

the pulse gas source inlet pipe is fixedly arranged on one side of the valve body and communicated with the gas cavity;

and the pulse liquid source connecting pipes are fixedly arranged on the corresponding one-way valves.

2. The MRI compatible ex vivo kidney perfusion system of claim 1, wherein an oxygenator is disposed on one side of the dish, an oxygenator inlet tube is fixedly mounted on one side of the oxygenator, and an oxygenator outlet tube is fixedly mounted on a top of the oxygenator.

3. The MRI compatible ex vivo kidney perfusion system of claim 1, wherein the holding vessel is fixedly mounted with a mixing channel on one side, and the mixing channel is fixedly mounted with a pressure sensor inlet tube on one side.

4. The MRI compatible ex vivo kidney perfusion system of claim 3, wherein a developer inlet tube is fixedly connected to the bottom of the liquid mixing channel, and a sampling valve is fixedly mounted to the top of the liquid mixing channel.

5. The MRI compatible ex vivo kidney perfusion system of claim 3, wherein a connecting tube is fixedly connected to the bottom of the liquid mixing channel, and a filter is fixedly mounted on the inner wall of the bottom of the holding vessel.

6. The MRI compatible in-vitro kidney perfusion system according to claim 1, wherein a protective shell is fixedly mounted at the bottom of the placing vessel, a cylinder is fixedly mounted on the inner wall of the bottom of the protective shell, a sliding cavity is formed in the cylinder, an air cavity is formed in the inner wall of the bottom of the sliding cavity, an air pressure pipe is fixedly mounted at the bottom of the cylinder, the bottom end of the air pressure pipe extends out of the protective shell, an air pressure valve is slidably mounted in the sliding cavity, a push rod is fixedly mounted at the top of the air pressure valve, and the top end of the push rod extends into the placing groove.

7. The MRI compatible ex vivo kidney perfusion system of claim 6, wherein two hollow tubes are fixedly mounted on the inner bottom wall of the protective shell, the top ends of the hollow tubes are fixedly connected with the bottom of the placing vessel, telescopic rods are slidably mounted in the hollow tubes, and the top ends of the two telescopic rods extend into the placing groove.

8. The MRI compatible ex vivo kidney perfusion system of claim 7, wherein a holding tray is fixedly mounted in the holding groove, the bottom of the holding tray is fixedly connected with the top ends of the top rod and the two telescopic rods, respectively, and an ex vivo kidney is placed on the holding tray.

9. The MRI compatible in vitro kidney perfusion system according to claim 7, wherein the hollow tube has sliding grooves formed on the inner walls of both sides thereof, the sliding grooves are slidably mounted with sliding blocks, and the sides of the two sliding blocks close to each other are fixedly connected with the telescopic rod.

10. The MRI compatible ex vivo kidney perfusion system of claim 7, wherein the inner wall of the bottom of the placement groove is provided with three sliding holes, isolating rings are arranged in the sliding holes, and the top rod and the two telescopic rods are respectively connected with the corresponding isolating rings in a sliding manner.

Technical Field

The invention relates to the technical field of kidney transplantation, in particular to an MRI (magnetic resonance imaging) -compatible in-vitro kidney perfusion system.

Background

The kidney transplantation technology is the most effective therapy in the end stage of kidney disease, along with the progress of immune tolerance technology, the application of regulatory T cells in the field of kidney transplantation and the effective control of cell-mediated rejection reaction, the survival rate of recipients after kidney transplantation is continuously improved, the kidney needs to be preserved before transplantation, the current clinical kidney supply mostly adopts a static cold storage method, the method has the advantages of simple operation, safe use, low price and better preservation effect, and a mechanical perfusion mode is adopted in partial areas.

However, in the prior art, the storage time of the static cold storage mode is short, the risk of delayed renal function transplantation recovery is high, while the mechanical perfusion mode has potential infection risk, and because the adopted full-flow perfusion mode does not have pulse perfusion simulating cardiac pacing, and under the MRI strong magnetic environment, the electromagnetic valve and the peristaltic pump cannot work normally, therefore, a new MRI compatible extracorporeal kidney perfusion system is needed to be provided to solve the technical problems.

Disclosure of Invention

The technical problem to be solved by the invention is to provide an MRI (magnetic resonance imaging) -compatible in vitro kidney perfusion system which can perform imaging scanning on an in vitro kidney without causing wound, can not cause infection to the kidney while performing pulse on the kidney and is more convenient to access the kidney.

To solve the above technical problems, the present invention provides an MRI compatible ex vivo renal perfusion system comprising: placing a dish; the placing groove is formed in the inner wall of the bottom of the placing vessel; an ex vivo kidney placed in a holding tank; the annular liquid storage cavity is formed in the placing vessel; the liquid storage inlet pipe is fixedly arranged on one side of the placing vessel and communicated with the annular liquid storage cavity; the liquid storage outlet pipe is fixedly arranged on one side of the placing vessel and communicated with the annular liquid storage cavity; the pulse air source generator is fixedly arranged on one side of the placing vessel; a valve body contained within a pulsed air source generator; the air cavity is arranged in the valve body; the exhaust hole is formed in the inner wall of one side of the air cavity; the silicone tube is fixedly arranged in the air cavity, and the top end and the bottom end of the silicone tube extend out of the air cavity; the two one-way valves are fixedly arranged at the top end and the bottom end of the silicone tube respectively; the pulse gas source inlet pipe is fixedly arranged on one side of the valve body and communicated with the gas cavity; and the pulse liquid source connecting pipes are fixedly arranged on the corresponding one-way valves.

Preferably, an oxygenator is arranged on one side of the placing vessel, an oxygenator inlet pipe is fixedly installed on one side of the oxygenator, and an oxygenator outlet pipe is fixedly installed on the top of the oxygenator.

Preferably, a liquid mixing passage is fixedly installed on one side of the placing vessel, and a pressure sensor inlet pipe is fixedly installed on one side of the liquid mixing passage.

Preferably, a developer inlet pipe is fixedly connected to the bottom of the liquid mixing passage, and a sampling valve is fixedly mounted on the top of the liquid mixing passage.

Preferably, the bottom of the liquid mixing passage is fixedly connected with a connecting pipe, and the inner wall of the bottom of the placing vessel is fixedly provided with a filter.

Compared with the related art, the MRI compatible in-vitro kidney perfusion system provided by the invention has the following beneficial effects:

the invention provides an MRI (magnetic resonance imaging) -compatible in-vitro kidney perfusion system.A circulating liquid is arranged in an annular liquid storage cavity, and the circulating liquid can be subjected to temperature control circulation through the matching of a liquid storage inlet pipe, a liquid storage outlet pipe and a temperature control unit so as to adjust the air flow of a diaphragm and the temperature of the circulating liquid; the perfusate is disconnected with all the control and monitoring unit probe diaphragms, so that the infection probability of the perfusate is reduced; the annular liquid storage cavity formed in the placing vessel is beneficial to the inlet and outlet of outside temperature control circulating liquid, meanwhile, the control unit is also far away from the placing vessel, the requirement of MRI scanning is met, the pulse air source generator can adjust the pulse pressure every time, and further the flow and the pressure of the perfusion liquid in the silica gel tube are controlled, so that the perfusion pressure and the flow can be adjusted according to different isolated kidneys; an ex vivo kidney perfusion system that can be run when MRI biopsies an ex vivo kidney.

To solve the above technical problems, the present invention provides an MRI compatible ex vivo renal perfusion system comprising: the protective housing, protective housing fixed mounting is in the bottom of placing the ware, fixed mounting has the cylinder on the bottom inner wall of protective housing, smooth chamber has been seted up on the cylinder, the air cavity has been seted up on the bottom inner wall in smooth chamber, the bottom fixed mounting of cylinder has the atmospheric pressure pipe, the bottom of atmospheric pressure pipe extends outside the protective housing, slidable mounting has the atmospheric pressure valve in the smooth chamber, the top fixed mounting of atmospheric pressure valve has the ejector pin, the top of ejector pin extends to in the standing groove.

Preferably, fixed mounting has two hollow tubes on the bottom inner wall of protecting crust, the top of hollow tube and the bottom fixed connection of placing the ware, slidable mounting has the telescopic link in the hollow tube, and the top of two telescopic links all extends to in the standing groove.

Preferably, fixed mounting has the holding dish in the standing groove, the bottom of holding dish respectively with the top fixed connection of ejector pin and two telescopic links, the separation kidney has been placed on the holding dish.

Preferably, the inner walls of the two sides of the hollow tube are provided with sliding grooves, sliding blocks are arranged in the sliding grooves in a sliding mode, and one sides, close to each other, of the two sliding blocks are fixedly connected with the telescopic rod.

Preferably, three sliding holes are formed in the inner wall of the bottom of the placing groove, isolating rings are arranged in the sliding holes, and the ejector rod and the two telescopic rods are respectively in sliding connection with the corresponding isolating rings.

Compared with the related art, the MRI compatible in-vitro kidney perfusion system provided by the invention has the following beneficial effects:

the invention provides an MRI (magnetic resonance imaging) -compatible in-vitro kidney perfusion system, which realizes the work of ascending and descending an in-vitro kidney by matching a sliding cavity, an air pressure pipe, an air pressure valve, an ejector rod and a containing disc, reduces the risk probability of the in-vitro kidney caused by inconvenience in taking, and ensures that the containing disc is more stable during ascending and descending by matching a hollow pipe and a telescopic rod.

Drawings

FIG. 1 is an elevational cross-sectional structural schematic view of a first embodiment of an MRI compatible ex vivo renal perfusion system provided by the present invention;

FIG. 2 is an assembly view of the pulsed air source generator;

FIG. 3 is a perspective assembly view of the pulsed air source generator;

FIG. 4 is a schematic top view of the present invention;

FIG. 5 is a schematic perspective view of the present invention;

FIG. 6 is a diagram of a fluid path pressure sensing channel of the present invention;

fig. 7 is a schematic front sectional view of a second embodiment of the present invention.

Reference numbers in the figures: 1. the device comprises a placing dish, 2, a placing groove, 3, an in vitro kidney, 4, an annular liquid storage cavity, 5, a liquid storage inlet pipe, 6, a liquid storage outlet pipe, 7, a pulse air source generator, 8, a valve body, 9, an air cavity, 10, an exhaust hole, 11, a silicone tube, 12, a one-way valve, 13, a pulse air source inlet pipe, 14, a pulse liquid source connecting pipe, 15, an oxygenator, 16, an oxygenator inlet pipe, 17, an oxygenator outlet pipe, 18, a liquid mixing passage, 19, a pressure sensor inlet pipe, 20, a developer inlet pipe, 21, a sampling valve, 22, a connecting pipe, 23, a filter, 24, a protective shell, 25, a column body, 26, a sliding cavity, 27, an air cavity, 28, an air pressure pipe, 29, an air pressure valve, 30, an ejector rod, 31, a hollow pipe, 32, a.

Detailed Description

The invention is further described with reference to the following figures and embodiments.

Accordingly, the present invention also provides an MRI compatible ex vivo renal perfusion system:

first embodiment

Referring to fig. 1-6 in combination, in a first embodiment of the present invention, an MRI compatible ex vivo renal perfusion system includes: placing a dish 1; the placing groove 2 is formed in the inner wall of the bottom of the placing vessel 1; an ex vivo kidney 3, the ex vivo kidney 3 being placed in the placement tank 2; the annular liquid storage cavity 4 is formed in the placing vessel 1, and the annular liquid storage cavity 4 is formed in the placing vessel 1; the liquid storage inlet pipe 5 is fixedly arranged on one side of the placing vessel 1, and the liquid storage inlet pipe 5 is communicated with the annular liquid storage cavity 4; the liquid storage outlet pipe 6 is fixedly arranged on one side of the placing vessel 1, and the liquid storage outlet pipe 6 is communicated with the annular liquid storage cavity 4; the pulse air source generator 7 is fixedly arranged on one side of the placing vessel 1; a valve body 8, said valve body 8 being contained within a pulsed air source generator 7; the air cavity 9, the said air cavity 9 is opened in the valve block 8; the exhaust hole 10 is formed in the inner wall of one side of the air cavity 9, and the exhaust hole 10 is formed in the inner wall of one side of the air cavity 9; the silicone tube 11 is fixedly arranged in the air cavity 9, and the top end and the bottom end of the silicone tube 11 both extend out of the air cavity 9; the two one-way valves 12 are respectively and fixedly arranged at the top end and the bottom end of the silicone tube 11; the pulse gas source inlet pipe 13 is fixedly arranged on one side of the valve body 8, and the pulse gas source inlet pipe 13 is communicated with the gas cavity 9; and the pulse liquid source connecting pipes 14 are fixedly arranged on the corresponding one-way valves 12.

An oxygenator 15 is arranged on one side of the placing dish 1, an oxygenator inlet pipe 16 is fixedly arranged on one side of the oxygenator 15, and an oxygenator outlet pipe 17 is fixedly arranged on the top of the oxygenator 15.

A liquid mixing passage 18 is fixedly arranged on one side of the placing dish 1, and a pressure sensor inlet pipe 19 is fixedly arranged on one side of the liquid mixing passage 18.

The bottom of the liquid mixing passage 18 is fixedly connected with a developer inlet pipe 20, and the top of the liquid mixing passage 18 is fixedly provided with a sampling valve 21.

The bottom of the liquid mixing passage 18 is fixedly connected with a connecting pipe 22, and the inner wall of the bottom of the placing vessel 1 is fixedly provided with a filter 23.

The working principle of the MRI compatible in vitro kidney perfusion system provided by the invention is as follows:

when the pulse gas source generator is used, the isolated kidney 3 is placed in the placing groove 2 at the bottom of the placing vessel 1, then perfusate is poured into the placing vessel 1, then the pulse power source is started, the perfusate is emptied and circulated to exhaust air in a pipeline, then the connecting pipe 22 is connected to the artery of the isolated kidney 3, and when the connecting pipe 22 is connected with the artery of the isolated kidney 3, the perfusate enters the silicone tube 11 in the pulse gas source generator 7 from the placing vessel 1 through the check valve 12 below;

at the moment, the pulse gas source enters the gas cavity 9 from the pulse gas source inlet pipe 13, the exhaust hole 10 does not exhaust the gas in time, at the moment, the gas compresses the silicone tube 11, so that the liquid in the silicone tube 11 flows in the direction of the one-way valve 12 at the top of the valve body 8, but can not return, when the gas in the air cavity 9 is exhausted through the exhaust hole 10 and the silicone tube 11 restores to be tubular, then the perfusion liquid outside the one-way valve 12 at the bottom of the valve body 8 is sucked into the section of the silicone tube 11 to wait for the next pulse air source and is repeated in sequence, thereby achieving the purpose that the diaphragm pushes the perfusion liquid to circulate, if the double kidneys need to be perfused simultaneously, a three-way pipe is connected to the connecting pipe 22 for double kidney simultaneous perfusion, an oxygenator 15 may be added to the perfusion procedure or imaging agent may be added through the imaging agent inlet tube 20 as needed to facilitate MRI biopsy of the isolated kidney 3.

Compared with the related art, the MRI compatible in-vitro kidney perfusion system provided by the invention has the following beneficial effects:

the invention provides an MRI (magnetic resonance imaging) -compatible in-vitro kidney perfusion system.A circulating liquid is arranged in an annular liquid storage cavity 4, and the circulating liquid can be subjected to temperature control circulation by matching a liquid storage inlet pipe 5, a liquid storage outlet pipe 6 and a temperature control unit so as to adjust the air flow of a diaphragm and the temperature of the circulating liquid; the perfusate is disconnected with all the control and monitoring unit probe diaphragms, so that the infection probability of the perfusate is reduced; the annular liquid storage cavity 4 formed in the placing vessel 1 is beneficial to the inlet and outlet of outside temperature control circulating liquid, meanwhile, the control unit is also far away from the placing vessel 1, the requirement of MRI scanning is met, the pulse air source generator 7 can adjust the pulse pressure every time, and further the flow and the pressure of the perfusion liquid in the silicone tube 11 are controlled, so that the perfusion pressure and the flow can be adjusted according to the difference of the in-vitro kidney 3; an ex vivo kidney 3 perfusion system that can be run when MRI biopsies are taken of the ex vivo kidney 3.

Second embodiment:

based on the MRI-compatible ex-vivo renal perfusion system provided in the first embodiment of the present application, a second embodiment of the present application proposes another MRI-compatible ex-vivo renal perfusion system, and the second embodiment is only a preferred way of the first embodiment, and the implementation of the second embodiment does not affect the implementation of the first embodiment alone.

The second embodiment of the present invention will be further explained with reference to the drawings and the embodiments

Referring to fig. 7 in combination, in a second embodiment of the invention, an MRI compatible ex vivo renal perfusion system includes: the protective shell 24, protective shell 24 fixed mounting is in the bottom of placing ware 1, fixed mounting has the cylinder 25 on the bottom inner wall of protective shell 24, smooth chamber 26 has been seted up on the cylinder 25, air cavity 27 has been seted up on the bottom inner wall of smooth chamber 26, the bottom fixed mounting of cylinder 25 has pneumatic tube 28, outside pneumatic tube 28's bottom extended to protective shell 24, slidable mounting has pneumatic valve 29 in smooth chamber 26, the top fixed mounting of pneumatic valve 29 has ejector pin 30, the top of ejector pin 30 extends to in the standing groove 2.

Fixed mounting has two hollow tubes 31 on the bottom inner wall of protecting crust, the top of hollow tube 31 and the bottom fixed connection who places the ware, slidable mounting has telescopic link 32 in hollow tube 31, and the top of two telescopic links 32 all extends to in the standing groove.

Fixed mounting has holding tray 33 in the standing groove 2, holding tray 33's bottom respectively with the top fixed connection of ejector pin 30 and two telescopic links 32, holding tray 33 is last to have placed separation kidney 3.

The spout has all been seted up on the both sides inner wall of hollow tube 31, slidable mounting has the slider in the spout, and one side that two sliders are close to each other all with telescopic link 32 fixed connection.

Three sliding holes are formed in the inner wall of the bottom of the placing groove 2, isolating rings are arranged in the sliding holes, and the ejector rod 30 and the two telescopic rods 32 are respectively in sliding connection with the corresponding isolating rings.

The working principle of the MRI compatible in vitro kidney perfusion system provided by the invention is as follows:

the pneumatic tube 28 is communicated with a pneumatic power source, the pneumatic tube 28 and the pulse gas source belong to two pneumatic pipelines respectively, when the device is used, gas enters the gas cavity 27 through the pneumatic tube 28, the gas starts to push the pneumatic valve 29 to ascend and enter the sliding cavity 26 under the continuous injection of the pneumatic power source, the pneumatic valve 29 also drives the ejector rod 30 to ascend while ascending in the sliding cavity 26, the ejector rod 30 drives the containing disc 33 to ascend through ascending, so that the containing disc 33 is lifted out of the placing groove 2, the placing or taking of the in-vitro kidney 3 is more convenient, and the risk probability of the in-vitro kidney 3 caused by inconvenience in taking is reduced;

meanwhile, when the kidney perfusion biopsy is carried out, the height of the in-vitro kidney 3 is adjusted, so that the FOV is coincided with the geometric center of the in-vitro kidney 3 during the MR I scanning, and the optimal detection image of the in-vitro kidney can be obtained.

Compared with the related art, the MRI compatible in-vitro kidney perfusion system provided by the invention has the following beneficial effects:

the invention provides an MRI (magnetic resonance imaging) -compatible in-vitro kidney perfusion system, which realizes the work of ascending and descending an in-vitro kidney 3 by matching a sliding cavity 26, an air cavity 27, a pneumatic tube 28, a pneumatic valve 29, an ejector rod 30 and a containing disc 33, reduces the risk probability of the in-vitro kidney 3 caused by inconvenient taking, and ensures that the containing disc 33 is more stable during ascending and descending by matching a hollow tube 31 and an expansion link 32.

It should be noted that the device structure and the accompanying drawings of the present invention mainly describe the principle of the present invention, and in the technology of the design principle, the arrangement of the power mechanism and the control system of the device, etc. is not completely described, but the details of the power mechanism and the control system can be clearly known by those skilled in the art on the premise of understanding the principle of the present invention.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.