阅读说明：本技术 一种抑制黑色素生成的美白冻干粉及其制备方法 (Whitening freeze-dried powder for inhibiting melanin generation and preparation method thereof ) 是由 不公告发明人 于 2019-12-10 设计创作，主要内容包括：本发明提供了一种抑制黑色素生成的美白冻干粉及其制备方法,以全蝎提取物、水蛭提取物、烟叶提取物、黄秋葵提取物、蛋清酶解液、山茶籽油、牛唾液提取液等为原料,经两次冷冻干燥得到。本发明所得冻干粉的皮肤吸收性好,可抑制黑色素生成,同时具有良好的保湿效果,从根本上实现皮肤美白,是一种理想的护肤美容产品。本发明的产品主要用于紧急补救性美容(快速美白),不需要长期使用。(The invention provides a whitening freeze-dried powder for inhibiting melanin generation and a preparation method thereof. The freeze-dried powder obtained by the invention has good skin absorbability, can inhibit melanin generation, has good moisturizing effect, fundamentally realizes skin whitening, and is an ideal skin-care and beauty-care product. The product of the invention is mainly used for emergency remedial beauty treatment (quick whitening) without long-term use.)

1. The preparation method of the skin-whitening freeze-dried powder for inhibiting melanin generation is characterized by comprising the steps of adding 1 part of scorpion extract, 0.2-0.3 part of hirudo extract, 3-5 parts of tobacco leaf extract and 0.2-0.3 part of okra extract into 1.5-2 parts of egg white enzymolysis liquid by weight, carrying out ultrasonic oscillation for 30-40 minutes, carrying out first freeze drying, taking out the mixture, adding the mixture into 2-3 parts of camellia seed oil, carrying out ultrasonic oscillation for 20-30 minutes to obtain a mixed solution, finally stirring at a stirring speed of 300-500 r/min while slowly dropwise adding the mixed solution into 5-8 parts of cattle saliva extracting solution, continuing stirring for 15-25 minutes after dropwise adding is finished, carrying out second freeze drying, and packaging to obtain the skin-whitening freeze-dried powder for inhibiting melanin generation.

2. The method of claim 1, wherein the Scorpio extract is prepared by the following steps: firstly, carrying out microwave treatment on whole live scorpions and crushing the whole live scorpions into powder, then adding the powder into a glycerol-acetic acid mixed solution with the weight 5-8 times of the weight of the powder, carrying out ultrasonic oscillation extraction for 5-8 hours, filtering to obtain a filtrate, concentrating the filtrate to 1/15-1/20 of the volume of the filtrate, then carrying out supercritical carbon dioxide extraction, wherein the pressure of a separation kettle is 7-8 MPa, the extraction temperature is 40-42 ℃, and the extraction time is 5-6 hours, thus obtaining the scorpio extract; the volume ratio of glycerol to acetic acid in the glycerol-acetic acid mixed solution is 1: 0.7 to 0.8.

3. The method of claim 1, wherein the leech extract is prepared by the following steps: taking the whole fresh leech, adding 3-6 times of water by weight, and homogenizing; taking homogenate, adjusting the pH to 7-9, adding 0.002-0.005 times of trypsin by weight, carrying out enzymolysis treatment at 40-60 ℃ for 8-12 hours, cooling to room temperature, adding 3-5 times of volume of absolute ethyl alcohol into the obtained enzymolysis liquid, standing and precipitating at 0-5 ℃ for 8-12 hours, filtering, and drying filtrate under reduced pressure to obtain the leech extract.

4. The preparation method according to claim 1, wherein the tobacco leaf extract is prepared by: the method comprises the steps of firstly, conducting enzyme deactivation, drying and crushing on tobacco leaves to prepare tobacco leaf pulp, then sequentially conducting complex enzyme enzymolysis and microwave extraction, conducting alcohol precipitation and then centrifuging to obtain an extract I and a supernatant, adding acetone with the weight being 3-5 times that of the extract I, conducting ultrasonic oscillation for 20-30 minutes, obtaining a precipitate through centrifugation to obtain an extract II, mixing the supernatant with chloroform with the same volume, conducting ultrasonic oscillation for 20-30 minutes, obtaining a precipitate through centrifugation to obtain an extract III, finally adding the extract II and the extract III into water with the weight being 5-8 times that of the total weight, conducting ultrasonic oscillation for 30-40 minutes, and obtaining the precipitate through centrifugation to obtain the tobacco leaf extract.

5. The method according to claim 1, wherein the okra extract is prepared by the following steps: transversely slicing cleaned and air-dried fresh okra, adding distilled water with the weight of 6-8 times of that of the okra, heating to boil, decocting for 30-40 minutes, centrifuging to obtain a supernatant, concentrating until the volume is reduced by 4/5-9/10 to obtain a concentrated solution, adding an ethanol aqueous solution with the volume concentration of 92-95% to ensure that the volume percentage of ethanol in the concentrated solution is 80-85%, standing at 0-5 ℃ for alcohol precipitation, centrifuging, collecting precipitates, and drying at 40-50 ℃ for 5-8 hours to obtain the okra extract.

6. The method according to claim 1, wherein the egg white enzymatic hydrolysate is prepared by: adding 0.01-0.02 times of neutral protease by weight into egg white liquid, uniformly stirring, carrying out enzymolysis for 3-4 hours at 50-53 ℃, adjusting the pH to 4-4.2 by using citric acid, heating and stirring for 15-20 minutes at 58-60 ℃, adjusting the pH to 6.8-7 by using triethanolamine, and filtering to obtain the egg white enzymolysis liquid.

7. The method of claim 1, wherein the camellia oil is prepared by the following steps: firstly, pulverizing camellia seeds into coarse camellia seed powder, then utilizing steam explosion technology and ultramicro nano pulverization to prepare fine camellia seed powder, and finally pressing by using a screw oil press to obtain the camellia seed oil.

8. The method according to claim 1, wherein the time required for dropping the mixture into the bovine saliva extract is 30 to 40 minutes, and the stirring rate during the dropping is 300 to 500 r/min.

9. The preparation method according to claim 1, wherein the first freeze-drying process conditions are as follows: freezing for 8-10 hours at-22 to-25 ℃, then vacuumizing to below 5Pa, maintaining the vacuum degree unchanged, heating to 28-32 ℃, and preserving heat for 3-5 hours; the process conditions of the second freeze drying are as follows: freezing for 7-9 hours at the temperature of-25 to-30 ℃, then vacuumizing to below 5Pa, maintaining the vacuum degree unchanged, heating to 30-35 ℃, and preserving heat for 8-10 hours.

10. A whitening freeze-dried powder for inhibiting melanin generation, which is obtained by the preparation method of any one of claims 1 to 9.

Technical Field

The invention relates to the technical field of beauty and skin care, and particularly relates to a whitening freeze-dried powder for inhibiting melanin generation and a preparation method thereof.

Background

With the improvement of living standard and aesthetic standard of people, white and clean skin is more and more advocated by most women, and the demand of whitening products is continuously increased. People want to obtain white and smooth skin by using whitening care products.

Research shows that skin pigmentation is caused by excessive melanin production in melanocytes, and if melanin production can be inhibited (tyrosinase is a rate-limiting enzyme for regulating melanin production), the skin whitening effect can be achieved fundamentally. Melanin is generated in melanocytes, and the melanocytes exist in the basal layer of the skin epidermis, so that functional components must permeate into the skin and reach the basal layer to exert the efficacy, which is a relatively difficult problem to solve, because the natural barrier of the horny layer is difficult to permeate, which is an important reason why the effects of many whitening skin care products are not ideal, and whether the product has good permeability is a main standard for judging whether the product is excellent.

The existing whitening agents for inhibiting melanin generation in the market mainly comprise AA2G, arbutin and derivatives thereof, kojic acid and derivatives thereof, vitamin C and derivatives thereof, endothelin antagonist, licoflavone, anthocyanin and the like. In any case, the absorption property is a large hindrance to the exertion of the efficacy.

In addition, the skin is white and bright, and the optical visual effect of the eyes is determined by the moisture retention of the skin and the fine degree of the skin besides the content and the uniform distribution of melanin. When sunlight irradiates on the skin, a part of the light firstly irradiates on the skin, and different skin is dull or bright and white due to the state of the skin and different angles for seeing the skin. If the skin is very smooth, the light beam bounces off in a regular direction, called specular reflection. If the eye receives this light exactly in the direction of reflection, otherwise it is not visible if the skin with which the light is in contact is rough, the light is totally null and the reflection in all directions is called diffuse reflection — the eye always receives a portion in whatever direction. The remaining part of the light encounters opaque components in the skin and is subsequently reflected, scattered in all directions, called scatter, and the eye receives a portion in all directions. And a part of the light is directly absorbed by various substances in the skin and disappears in the skin. Therefore, we see the skin as the sum of specular reflection (only at certain angles) + diffuse reflection + scattered light. The higher this total amount, the brighter the skin appears. Therefore, the moisture retention of skin also directly affects whether the skin is white, and the existing whitening products are only aimed at whitening. In the skin whitening process, the metabolic circulation of the skin is promoted, a large amount of water is consumed, and the skin is dry, and the skin whitening product and the moisturizing product are usually needed to be matched in the actual skin care process, so that the skin whitening process is undoubtedly complicated.

The freeze-dried powder is a common skin care and beauty product, and is prepared by freezing a material solution to be dried into a solid in advance, and then directly subliming to remove water from the frozen state without passing through a liquid state under the conditions of low temperature and low pressure. The lyophilized powder well ensures the high activity of the original cosmetic components, achieves ultrahigh stability, is mixed with lysozyme, is quickly recovered and is convenient to use. High activity, ensures the nutrient components of the product, can be better absorbed by cells, thereby shortening and improving the skin cycle.

Disclosure of Invention

The invention aims to provide whitening freeze-dried powder for inhibiting melanin generation and a preparation method thereof, and aims to solve the technical problems that the whitening effect is influenced due to poor skin absorbability, the moisturizing performance of whitening products is not enough, and the like.

In order to achieve the purpose, the invention provides a preparation method of a whitening freeze-dried powder for inhibiting melanin generation, which comprises the steps of adding 1 part of scorpion extract, 0.2-0.3 part of hirudo extract, 3-5 parts of tobacco leaf extract and 0.2-0.3 part of okra extract into 1.5-2 parts of egg white enzymatic hydrolysate by weight, carrying out ultrasonic oscillation for 30-40 minutes, carrying out first freeze drying, taking out, adding the mixture into 2-3 parts of camellia seed oil, carrying out ultrasonic oscillation for 20-30 minutes to obtain a mixed solution, finally stirring at a stirring speed of 300-500 r/min, slowly dripping the mixed solution into 5-8 parts of bovine saliva extract, continuing stirring for 15-25 minutes after dripping is finished, carrying out second freeze drying, and packaging to obtain the whitening freeze-dried powder for inhibiting melanin generation.

Preferably, the preparation method of the scorpion extract comprises the following steps: firstly, carrying out microwave treatment on whole live scorpions and crushing the whole live scorpions into powder, then adding the powder into a glycerol-acetic acid mixed solution with the weight 5-8 times of the weight of the powder, carrying out ultrasonic oscillation extraction for 5-8 hours, filtering to obtain a filtrate, concentrating the filtrate to 1/15-1/20 of the volume of the filtrate, then carrying out supercritical carbon dioxide extraction, wherein the pressure of a separation kettle is 7-8 MPa, the extraction temperature is 40-42 ℃, and the extraction time is 5-6 hours, thus obtaining the scorpio extract; the volume ratio of glycerol to acetic acid in the glycerol-acetic acid mixed solution is 1: 0.7 to 0.8.

Further preferably, the specific method for microwave treatment and pulverization into powder comprises the following steps: the method comprises the steps of firstly treating the scorpion in a microwave for 3-5 minutes at 300-350W, and then crushing to 300-400 meshes.

Preferably, the preparation method of the leech extract comprises the following steps: taking the whole fresh leech, adding 3-6 times of water by weight, and homogenizing; taking homogenate, adjusting the pH to 7-9, adding 0.002-0.005 times of trypsin by weight, carrying out enzymolysis treatment at 40-60 ℃ for 8-12 hours, cooling to room temperature, adding 3-5 times of volume of absolute ethyl alcohol into the obtained enzymolysis liquid, standing and precipitating at 0-5 ℃ for 8-12 hours, filtering, and drying filtrate under reduced pressure to obtain the leech extract.

Preferably, the preparation method of the tobacco leaf extract comprises the following steps: the method comprises the steps of firstly, conducting enzyme deactivation, drying and crushing on tobacco leaves to prepare tobacco leaf pulp, then sequentially conducting complex enzyme enzymolysis and microwave extraction, conducting alcohol precipitation and then centrifuging to obtain an extract I and a supernatant, adding acetone with the weight being 3-5 times that of the extract I, conducting ultrasonic oscillation for 20-30 minutes, obtaining a precipitate through centrifugation to obtain an extract II, mixing the supernatant with chloroform with the same volume, conducting ultrasonic oscillation for 20-30 minutes, obtaining a precipitate through centrifugation to obtain an extract III, finally adding the extract II and the extract III into water with the weight being 5-8 times that of the total weight, conducting ultrasonic oscillation for 30-40 minutes, and obtaining the precipitate through centrifugation to obtain the tobacco leaf extract.

Preferably, the tobacco leaf pulp is obtained by mixing the crushed tobacco powder with water in an amount of 8-10 times of the weight of the crushed tobacco powder and grinding the mixture into pulp; the specific method of compound enzyme enzymolysis is as follows: adding a complex enzyme into tobacco leaf slurry 200-250 times of the weight of the tobacco leaf slurry, and carrying out enzymolysis treatment at 50-60 ℃ for 2-3 hours, wherein the complex enzyme is prepared by mixing cellulase and pectinase according to a mass ratio of 0.8-1: 1, mixing to obtain; the process conditions of microwave extraction are as follows: microwave treatment is carried out for 3-5 minutes at 300-500W.

Preferably, the preparation method of the okra extract comprises the following steps: transversely slicing cleaned and air-dried fresh okra, adding distilled water with the weight of 6-8 times of that of the okra, heating to boil, decocting for 30-40 minutes, centrifuging to obtain a supernatant, concentrating until the volume is reduced by 4/5-9/10 to obtain a concentrated solution, adding an ethanol aqueous solution with the volume concentration of 92-95% to ensure that the volume percentage of ethanol in the concentrated solution is 80-85%, standing at 0-5 ℃ for alcohol precipitation, centrifuging, collecting precipitates, and drying at 40-50 ℃ for 5-8 hours to obtain the okra extract.

Preferably, the preparation method of the egg white enzymolysis liquid comprises the following steps: adding 0.01-0.02 times of neutral protease by weight into egg white liquid, uniformly stirring, carrying out enzymolysis for 3-4 hours at 50-53 ℃, adjusting the pH to 4-4.2 by using citric acid, heating and stirring for 15-20 minutes at 58-60 ℃, adjusting the pH to 6.8-7 by using triethanolamine, and filtering to obtain the egg white enzymolysis liquid.

Preferably, the preparation method of the camellia seed oil comprises the following steps: firstly, pulverizing camellia seeds into coarse camellia seed powder, then utilizing steam explosion technology and ultramicro nano pulverization to prepare fine camellia seed powder, and finally pressing by using a screw oil press to obtain the camellia seed oil.

Further preferably, the process conditions of the steam explosion technology are as follows: filling camellia seed coarse powder into a blasting cavity of steam blasting equipment, keeping the camellia seed coarse powder for 1-2 minutes under the condition of 1-2.5 MPa, and then quickly releasing pressure; carrying out ultra-micro nano-pulverization by using a jet mill, wherein the working conditions are as follows: the airflow pressure is 1100kPa, the feeding speed is 180r/min, the grading frequency is 35Hz, and the crushing time is 60-80 minutes; the working conditions of the screw oil press are as follows: and idling the screw oil press for 3-5 minutes, feeding camellia seed fine powder while the oil press is hot, and starting squeezing when the temperature of the oil press reaches 70-80 ℃ until no oil flows out.

Preferably, the preparation method of the cattle saliva extracting solution comprises the following steps: collecting saliva of healthy cattle, centrifuging and taking supernatant to obtain the cattle saliva extracting solution.

Preferably, the time required for dripping the mixed solution into the cattle saliva extracting solution is 30-40 minutes, and the stirring speed in the dripping process is 300-500 r/min.

Preferably, the process conditions of the first freeze drying are as follows: freezing for 8-10 hours at-22 to-25 ℃, then vacuumizing to below 5Pa, maintaining the vacuum degree unchanged, heating to 28-32 ℃, and preserving heat for 3-5 hours; the process conditions of the second freeze drying are as follows: freezing for 7-9 hours at the temperature of-25 to-30 ℃, then vacuumizing to below 5Pa, maintaining the vacuum degree unchanged, heating to 30-35 ℃, and preserving heat for 8-10 hours.

Preferably, the specific method of packaging is as follows: subpackaging with a penicillin bottle of 10mL specification, and sealing with a gland.

The whitening freeze-dried powder for inhibiting melanin generation is obtained by the preparation method.

The invention has the following beneficial effects:

the invention takes scorpion extract, leech extract, tobacco leaf extract, okra extract, egg white enzymolysis liquid, camellia seed oil, cow saliva extract and the like as raw materials, and freeze-dried powder products are obtained by freeze drying twice, and the freeze-dried powder products have good skin absorbability, can inhibit melanin generation, simultaneously have good moisturizing effect, fundamentally realize skin whitening, and are ideal skin-care and beauty-care products. The product of the invention is mainly used for emergency remedial beauty treatment (quick whitening) without long-term use. The advantages of the invention are specifically analyzed as follows:

1. the method comprises the steps of mixing a scorpion extract, a leech extract, a tobacco leaf extract, an okra extract and egg white enzymolysis liquid, carrying out primary freeze drying, adding the mixture into camellia seed oil to generate small oil drops of fat-soluble coating powder, slowly dripping the small oil drops into a cattle saliva extracting solution to microscopically present an oil-in-water state, and carrying out secondary freeze drying after full dispersion. When the obtained freeze-dried powder product is mixed with a solvent, the freeze-dried powder product still can be oil-in-water, the skin feeling is fresh, the moisture retention of the conventional oil-in-water is inferior to that of water-in-oil, but the cattle saliva extract component with the oil-in-water outer layer structure is constructed in the invention, and the cattle saliva extract component contains abundant mucopolysaccharide and has good moisture retention.

2. The scorpion extract contains rich mineral substances, betaine, palmitic acid, oleic acid and the like, and the mineral substances can enable the skin to absorb water better and faster and enhance the absorption capacity of the skin; the betaine allows water molecules to be completely absorbed and utilized by the whole living cells, has a unique moisturizing effect and has an antiallergic effect; the palmitic acid is excellent in skin softness and moisture, has good permeability, can permeate keratinocytes and can promote the absorption of other components by the skin; oleic acid can complex copper, and copper ions are required for the activity of tyrosinase, so that the existence of oleic acid inhibits the activity of tyrosinase, inhibits melanin synthesis and has the whitening effect.

3. The leech extract contains hirudin which is polypeptide consisting of 65 amino acids, has small molecular weight, can be quickly absorbed by skin, can quickly dilute blood viscosity, accelerate blood circulation and dredge capillary vessels, can promote deeper absorption below the skin horny layer on the basis that the palmitic acid in the scorpion extract promotes the penetration of the horny layer, and synergistically improves the absorbability of the skin by combining the improvement of mineral substances in the scorpion extract on the absorption capacity of the skin, thereby exerting the maximum efficacy of moisturizing and whitening components. The skin is not smooth in local microcirculation, skin nutrition is derived from blood transportation, and the leech extract can directly provide nutrition, improve microcirculation of skin capillaries, improve utilization rate of nutritional ingredients such as the scorpion extract, the tobacco leaf extract, the okra extract and egg white, and better play whitening and moisturizing effects.

4. The tobacco leaf extract contains suberic acid and polysaccharide substances, and the suberic acid has certain structural similarity with a substrate tyrosine of tyrosinase, so that the activity of the tyrosinase can be competitively inhibited, the formation of melanin is influenced, and the whitening effect is achieved; the polysaccharides have moisture keeping and antioxidant effects. Because the tobacco leaf extract contains nicotine, solanesol and other substances which are not good for human bodies, the substances are respectively removed by a chloroform and acetone dissolving method in the process of extracting the tobacco leaves.

5. The okra extract contains abundant pectin components, has good film forming property, forms a natural barrier for skin, avoids ultraviolet ray damage, prevents water molecules from escaping, and enhances the moisturizing property of the moisturizing components.

6. The egg white contains active sulfydryl, which can inhibit the activity of tyrosinase, thereby inhibiting the generation of melanin and whitening skin. Because the egg white contains macromolecular proteins and cannot be absorbed by the skin, the invention degrades the macromolecular proteins through enzymolysis treatment to obtain the polypeptide or amino acid which can be absorbed by the skin, thereby being beneficial to the skin absorption.

The whitening effect of the invention mainly depends on the synergistic effect of the scorpion extract, the tobacco leaf extract and the egg white. The existing research proves that tyrosinase is the rate-limiting enzyme for regulating and controlling melanin generation, so that the whitening can be fundamentally realized by inhibiting the activity of tyrosinase. The suberic acid in the tobacco leaf extract has certain similarity with the tyrosine structure, so that the combination process of tyrosinase and tyrosine is interfered, the activity of the tyrosinase on a substrate to generate melanin is greatly inhibited, but a part of the tyrosinase can be combined with the tyrosine. The tyrosinase contains hydroxyl and amino, and sulfhydryl in egg white can form hydrogen bond with hydroxyl, amino and the like to prevent the residual tyrosinase from being combined with tyrosine. Because the hydrogen bond energy formed by sulfydryl is smaller, in order to strengthen the inhibition effect on the tyrosinase activity, the scorpion extract is introduced, the oleic acid contained in the scorpion extract has a binding effect on copper ions, and the activity of tyrosinase requires the copper ions, so that the activity of the tyrosinase is comprehensively inhibited.

The moisturizing effect of the invention mainly depends on the synergistic effect of the scorpion extract, the tobacco leaf extract and the okra extract. Mineral substances in the scorpion extract promote water absorption, water molecules are completely absorbed and utilized by living cells under the action of betaine, hydroxyl contained in polysaccharide in the tobacco leaf extract can be combined with the water molecules to form hydrogen bonds, the water molecules are retained under the action of the hydrogen bonds, the internal moisturizing effect is achieved, the water molecules which cannot form the hydrogen bonds escape in time can be shielded by pectin films in the okra extract and return again to be absorbed and utilized by the living cells, all parts cooperate with each other, and the moisture retention effect is good. 7. The camellia seed oil is similar to skin oil, so that the freeze-dried powder product has better compatibility with the skin, plays a pioneer guiding role, opens an absorption channel for the absorption of minerals, hirudin and the like, promotes the absorbability of other components, and on the other hand, constructs an oil core of oil-in-water and plays a good role in wrapping water-insoluble components.

8. Besides containing a large amount of mucopolysaccharide, the cattle saliva extract also contains urea, free amino acids, mineral substances and the like, wherein the urea is beneficial to reducing the water loss of the skin, has good water locking capacity and enhancing the moisture retention performance, and the amino acids, the mineral substances and the like can provide nutrition support for the skin and improve the skin condition.

In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below.

Detailed Description

The following is a detailed description of embodiments of the invention, but the invention can be implemented in many different ways, as defined and covered by the claims.