阅读说明：本技术 基于聚合物复合膜快速制备单层dmf芯片及制备方法 (Single-layer DMF (dimethyl formamide) chip quickly prepared based on polymer composite membrane and preparation method ) 是由 叶尊忠 王振 应义斌 吴翠 应圣纳 于 2019-09-30 设计创作，主要内容包括：本发明公开了一种基于聚合物复合膜快速制备单层DMF芯片及制备方法。芯片包括下上层叠的电极阵列层、介电层和疏水层,介电层包括上下层叠的聚合物复合膜基材层和聚合物复合膜粘着剂层；电极阵列层包括印刷电路板基板和电极阵列,电极阵列是由多个驱动电极单元阵列布置构成,驱动电极单元中间设电路板过孔,电路板过孔贯穿于印刷电路板基板周围布置焊盘；介电疏水复合层贴附于电极阵列上面；介电疏水复合层上设有试剂液滴,向各个驱动电极单元施加不同/相同的电压带动试剂液滴在介电疏水复合层上快速运动。本发明提出的工艺可以大大降低数字微流芯片制备成本,避免材料表面吸附生物试剂带来交叉感染的可能,提高微流控的灵活性和稳定性。(The invention discloses a monolayer DMF chip prepared rapidly based on a polymer composite membrane and a preparation method thereof. The chip comprises an electrode array layer, a dielectric layer and a hydrophobic layer which are stacked from bottom to top, wherein the dielectric layer comprises a polymer composite film base material layer and a polymer composite film adhesive layer which are stacked from top to bottom; the electrode array layer comprises a printed circuit board substrate and an electrode array, the electrode array is formed by arranging a plurality of driving electrode unit arrays, a circuit board through hole is arranged in the middle of each driving electrode unit, and the circuit board through hole penetrates through a bonding pad arranged around the printed circuit board substrate; the dielectric hydrophobic composite layer is attached to the electrode array; reagent droplets are arranged on the dielectric hydrophobic composite layer, and different/same voltages are applied to all the driving electrode units to drive the reagent droplets to rapidly move on the dielectric hydrophobic composite layer. The process provided by the invention can greatly reduce the preparation cost of the digital microfluidic chip, avoid the possibility of cross infection caused by biological reagent adsorbed on the surface of the material, and improve the flexibility and stability of the microfluidic.)

1. A fast preparation monolayer DMF chip based on polymer composite membrane is characterized in that: the electrode array layer structure comprises an electrode array layer (5), a dielectric layer (9) and a hydrophobic layer (6) which are sequentially stacked from bottom to top, wherein the hydrophobic layer (6) is arranged on the dielectric layer (9), the dielectric layer (9) is arranged on the electrode array layer (5), and the dielectric layer (9) comprises a polymer composite film base material layer (7) and a polymer composite film adhesive layer (8) which are stacked from top to bottom; the electrode array layer (5) comprises a printed circuit board substrate (3) and an electrode array (10) arranged on the upper surface of the printed circuit board substrate (3), the electrode array (10) is formed by arranging a plurality of driving electrode units (4) in an array manner, an electrode gap is formed between every two adjacent driving electrode units (4), a circuit board through hole (1) is formed in the middle of each driving electrode unit (4), and after the circuit board through hole (1) penetrates through the printed circuit board substrate (3), a bonding pad (2) is arranged on the lower surface of the printed circuit board substrate (3) around the circuit board through hole (1); the dielectric layer (9) is completely attached to the electrode array (10), and the hydrophobic layer (6) is completely attached to the dielectric layer (9); reagent droplets (11) are arranged on the upper surface of the hydrophobic layer (6), each driving electrode unit (4) of the electrode array (10) is led out through a circuit board through hole (1) to be connected to an external voltage control end, and the voltage control end applies different/same voltages to each driving electrode unit (4) at different moments in real time to drive the reagent droplets (11) to rapidly move on the hydrophobic layer (6), so that the micro-fluidic chip works.

2. The single-layer DMF chip based on the polymer composite membrane of claim 1, which is characterized in that: the driving electrode unit (4) is a square electrode plate, and the electrode array (10) is formed by arranging a plurality of square electrode plates in a square array.

3. The single-layer DMF chip based on the polymer composite membrane of claim 1, which is characterized in that: the driving electrode unit (4) is a square electrode plate with four straight edges replacing a zigzag edge or a wavy edge, and the electrode array (10) is formed by arranging a plurality of square electrode plates in a square array.

4. The single-layer DMF chip based on the polymer composite membrane of claim 1, which is characterized in that: the driving electrode unit (4) is a triangular electrode plate with three linear edges replacing fold line edges or wave edges, and the electrode array (10) is formed by arranging a plurality of triangular electrode plates in a triangular array.

5. The single-layer DMF chip based on the polymer composite membrane of claim 1, which is characterized in that: the driving electrode unit (4) is a hexagonal electrode plate with six linear edges replacing fold line edges or wave edges, and the electrode array (10) is formed by arranging a plurality of hexagonal electrode plates in a honeycomb array.

6. The single-layer DMF chip based on the polymer composite membrane of claim 1, which is characterized in that: the hydrophobic layer (6) adopts paint with hydrophobic property.

7. The single-layer DMF chip based on the polymer composite membrane of claim 1, which is characterized in that: and a layer of oil film is further coated on the hydrophobic layer (6), and the oil film material is selected from silicone oil, paraffin oil or edible oil and the like.

8. The single-layer DMF chip based on the polymer composite membrane of claim 1, which is characterized in that: in the dielectric layer (9), the polymer composite film base material layer (7) is made of polymerized materials such as BOPP (polypropylene), PET (polyester), PVC (polyvinyl chloride), PE (polyethylene) and the like, and the polymer composite film adhesive layer (8) is made of materials such as release paper, release film and the like.

9. A method for preparing a single-layer digital microfluidic chip according to any one of claims 1 to 8, comprising: the preparation method comprises the following steps: an industrial printed circuit board preparation method is adopted by an electrode array layer (5), a polymer composite film adhesive layer (8) and a polymer composite film base material layer (7) are sequentially attached to the upper surface of the electrode array layer (5) through an attaching method, and then a single-layer hydrophobic material prepared in advance is flatly attached to the upper surface of the polymer composite film base material layer (7) to form a hydrophobic layer (6).

10. The method for preparing the single-layer DMF chip based on the polymer composite membrane according to claim 9, which is characterized in that: and a layer of oil film is further coated on the upper surface of the hydrophobic layer (6), and the oil film material is selected from silicone oil, paraffin oil or edible oil and the like.

Technical Field

The invention relates to a digital microfluidic chip and a preparation method thereof in the field of microfluidics, in particular to a single-layer DMF (digital microfluidic) chip quickly prepared based on a polymer composite film and a preparation method thereof.

Background

At present, when related biochemical experiments are involved in the fields of drug research and development, disease detection, gene detection and the like, experimenters are required to use tools such as pipette guns, kits, test tubes and the like to carry out experiments. The use of a large number of repeated steps and test reagents results in a great waste of test resources. Different channels are designed on a chip by the micro-fluidic technology to realize the mixed reaction function of liquid, reaction and detection steps can be concentrated on one chip by combining a certain detection means, the volume of reaction liquid drops is reduced to nano-liter or even pico-liter level, the reaction liquid drops are controlled by controlling the electric field change of the chip to independently complete the experiment, the experiment steps, the reagent consumption and the labor input are greatly reduced, and the method is an effective means for reducing the experiment cost.

The digital microfluidic technology can independently control liquid drops by using an electric field, and is different from the traditional continuous microfluidic technology which uses a micro-valve micropump to control liquid or air pressure to realize reaction liquid conveying. The digital microfluidic chip can realize the transportation, fusion, division and distribution of liquid drops, and can realize the automation of complex biochemical tests by controlling the electric field of the electrode array and combining with a proper detection means.

The traditional preparation method of the digital microfluidic chip adopts an MEMS (micro electro mechanical systems) manufacturing process, which has strict requirements on preparation environment, expensive price of required equipment and complex manufacturing process, and is not suitable for mass production and application. In addition, the digital microfluidic chip prepared by the traditional MEMS process has some problems in chip recycling. In the experimental process, the dielectric layer is easy to break down or biological pollution occurs to cause that the chip can not be used continuously, the experimental cost is further improved, and the subsequent practical application is not facilitated.

The preparation process of the existing digital microfluidic chip needs to realize the preparation of electrodes by a photoetching technology, and a matched instrument and a super clean room are needed, so that the preparation condition of the chip is improved. The traditional chip respectively comprises a dielectric layer and a hydrophobic layer, wherein each layer is prepared by a spin coating process, the steps are complex, and if the thickness of an electrode is too large, the dielectric layer and the hydrophobic layer can form a gully, which affects the stability of droplet movement.

Disclosure of Invention

The invention aims to provide a single-layer digital microfluidic chip prepared quickly based on a polymer composite film and a preparation method thereof, aiming at overcoming the defects of high manufacturing cost, complex processing steps and the like of the traditional digital microfluidic manufacturing process. The chip adopts the polymer composite film as the dielectric layer, and has the characteristics of quick preparation and low cost.

The technical scheme of the invention is as follows:

one is based on the quick preparation monolayer DMF chip of polymer composite film:

the chip comprises an electrode array layer, a dielectric layer and a hydrophobic layer which are sequentially arranged in a laminated manner from bottom to top, the hydrophobic layer is arranged on the dielectric layer, the dielectric layer is arranged on the electrode array layer, and the dielectric layer comprises a polymer composite film base material layer and a polymer composite film adhesive layer which are arranged in a laminated manner from top to bottom; the electrode array layer comprises a printed circuit board substrate and an electrode array arranged on the upper surface of the printed circuit board substrate, the electrode array is formed by arranging a plurality of driving electrode unit arrays, an electrode gap is formed between every two adjacent driving electrode units, a circuit board through hole is arranged in the middle of each driving electrode unit, and after the circuit board through hole penetrates through the printed circuit board substrate, a bonding pad is arranged on the lower surface of the printed circuit board substrate around the circuit board through hole; the dielectric layer is completely attached to the electrode array, and the hydrophobic layer is completely attached to the dielectric layer; reagent droplets are arranged on the upper surface of the hydrophobic layer, each driving electrode unit of the electrode array is led out through a circuit board via hole to be connected to an external voltage control end, and the voltage control end applies different/same voltages to each driving electrode unit at different moments in real time to drive the reagent droplets to rapidly move on the hydrophobic layer, so that the work of the microfluidic chip is realized.

The driving electrode unit is a square electrode plate, and the electrode array is formed by arranging a plurality of square electrode plates in a square array.

The driving electrode unit is a square electrode plate with four linear edges replacing fold line edges or wave edges, and the electrode array is formed by arranging a plurality of square electrode plates in a square array.

The driving electrode unit is a triangular electrode plate with three linear edges replacing fold line edges or wave edges, and the electrode array is formed by arranging a plurality of triangular electrode plates in a triangular array.

The drive electrode unit is a hexagonal electrode plate with six linear edges replacing fold line edges or wave edges, and the electrode array is formed by arranging a plurality of hexagonal electrode plates in a honeycomb array.

The hydrophobic layer is made of paint with hydrophobic property, such as Teflon, Cytop and the like.

And a layer of oil film is further coated on the hydrophobic layer, and the oil film material is selected from silicone oil, paraffin oil or edible oil and the like.

In the dielectric layer, the polymer composite film base material layer is made of polymerized materials such as BOPP (polypropylene), PET (polyester), PVC (polyvinyl chloride) and PE (polyethylene), and the polymer composite film adhesive layer is made of materials such as release paper and release film.

The adhesive layer is adhered to the base material layer to form a layer and has certain viscosity.

Secondly, a preparation method of the single-layer digital microfluidic chip comprises the following steps: the electrode array layer is prepared by adopting an industrial printed circuit board preparation method, a polymer composite film adhesive layer and a polymer composite film base material layer are sequentially attached to the upper surface of the electrode array layer by an attaching method, and then a single-layer hydrophobic material prepared in advance is flatly attached to the upper surface of the polymer composite film base material layer to form a hydrophobic layer.

And further coating a layer of oil film on the upper surface of the hydrophobic layer, wherein the oil film is made of silicone oil, paraffin oil or edible oil.

The digital microfluidic chip provided by the invention has a single-layer structure, namely, a reagent droplet only moves on one chip.

The invention has the beneficial effects that:

compared with the traditional MEMS processing technology, the preparation method of the digital microfluidic chip provided by the invention is not required to be finished in a clean room, and equipment such as sputtering coating and the like is not required. The dielectric layer of the digital microfluidic chip is formed by attaching a polymer composite film, and complex operations such as spin coating, vapor deposition and the like are not needed. The chip manufacturing steps are greatly simplified, the chip material is easy to obtain, and the manufacturing cost is reduced. The digital microfluidic chip prepared by the process can be reused, and the experiment cost can be further reduced.

Meanwhile, the invention can lower and simplify the cost of replacing the unit when the voltage breaks down the film, and realize low-cost reuse.

Drawings

FIG. 1 is a cross-sectional view of a single-layer digital microfluidic chip according to the present invention;

FIG. 2 is a top view of a single-layer digital microfluidic chip according to the present invention;

FIG. 3 is a structural diagram of a driving electrode unit formed by a fold line-shaped edge; in FIG. 3, from (a) to (c) are respectively a regular triangle, a square and a regular hexagon composed of zigzag lines;

FIG. 4 is a view of a driving electrode unit constructed with wavy edges; in fig. 4, from (a) to (c) are respectively a regular triangle, a square and a regular hexagon composed of wavy lines;

FIG. 5 is an electrode array formed by regular triangles, squares and regular hexagons of the zigzag edge-driven electrode units;

fig. 6 shows an electrode array consisting of regular triangles, squares and hexagons of the wave-shaped edge driving electrode unit.

In the figure: the circuit board comprises a circuit board through hole 1, a bonding pad 2, a printed circuit board substrate 3, a driving electrode unit 4, an electrode array layer 5, a hydrophobic layer 6, a polymer composite film substrate layer 7, a polymer composite film adhesive layer 8, a dielectric layer 9, an electrode array 10 and reagent droplets 11.

Detailed Description

The invention will be further described with reference to the accompanying drawings, but the invention is not limited to the following examples.

As shown in fig. 1, the specific implementation includes an electrode array layer 5, a dielectric layer 9 and a hydrophobic layer 6 which are sequentially stacked from bottom to top, wherein the hydrophobic layer 6 is disposed on the dielectric layer 9, the dielectric layer 9 is disposed on the electrode array layer 5, and the dielectric layer 9 includes a polymer composite film base material layer 7 and a polymer composite film adhesive layer 8 which are stacked from top to bottom; the electrode array layer 5 comprises a printed circuit board substrate 3 and an electrode array 10 arranged on the upper surface of the printed circuit board substrate 3, the electrode array 10 is formed by arranging a plurality of driving electrode units 4 in an array manner, an electrode gap is formed between every two adjacent driving electrode units 4, a circuit board through hole 1 is arranged in the middle of each driving electrode unit 4, after the circuit board through hole 1 penetrates through the printed circuit board substrate 3, a bonding pad 2 is arranged on the lower surface of the printed circuit board substrate 3 around the circuit board through hole 1, and the driving electrode units 4 are connected to a through hole bonding pad 2 below the printed circuit board substrate 3 through the circuit board through holes 1; the dielectric layer 9 is completely attached to the electrode array 10, and the hydrophobic layer 6 is completely attached to the dielectric layer 9; reagent droplets 11 are arranged on the upper surface of the hydrophobic layer 6, each driving electrode unit 4 of the electrode array 10 is led out through a circuit board via hole 1 and connected to an external voltage control end, and the voltage control end applies different/same voltages to each driving electrode unit 4 at different moments in real time to drive the reagent droplets 11 to rapidly move on the hydrophobic layer 6, so that the work of the microfluidic chip is realized.

The reagent droplets 11 were prepared from a 0.2mol/L KCl solution, and the volume of each droplet was 5. mu.L.

A plurality of reagent droplets 11 may be provided on the hydrophobic layer 6. In the case that the reagent droplet 11 may be a plurality of droplets composed of a plurality of components, a plurality of reagents may be selected as different droplets to participate in the experiment according to the experiment requirement. The plurality of reagent droplets 11 may each control the movement of the droplet by applying an electric field across electrodes in the vicinity of the droplet. After an electric field is applied to the electrode unit 14 near the droplet 11, the droplet moves toward the electrode unit 14 under the action of the electric field, i.e., a transport step in the droplet manipulation step. In this way, the two liquid drops can be controlled to move to the same electrode to complete the fusion step. After the electric field is applied simultaneously by the electrode driving units 14, 15, 16 near the droplet 11, the step of splitting the droplet, i.e. splitting one droplet into two droplets, can be realized by turning off the electric field of the driving unit 15. The design of complex experimental schemes can be realized through the mutual cooperation of the liquid drop conveying, fusing and splitting steps.

The electrode array layer 5 adopts an industrial printed circuit board process; the dielectric layer 9 and the hydrophobic layer 6 are sequentially attached on the electrode array layer 5 by an attaching method.