阅读说明：本技术 一种黑腹果蝇引诱剂及其应用 (Drosophila melanogaster attractant and application thereof ) 是由 刘永强 王忠跃 蔡欢欢 胡西旦·买买提 张晓芳 于 2021-03-12 设计创作，主要内容包括：本发明公开了一种黑腹果蝇引诱剂及其应用。该黑腹果蝇引诱剂,其活性成分包括醋酸异戊酯,优选的,其活性成分由醋酸异戊酯和棕榈酸乙酯组成,其中醋酸异戊酯与棕榈酸乙酯的重量份数比为1～10：1～10。本发明果蝇引诱剂配方,具有专一性强,引诱效果好,组分释放持效期长等优点。本发明的实施可以降低黑腹果蝇对我国水果生产的为害程度,有效控制害虫虫口密度,具有良好的经济效益和环境效益。(The invention discloses a drosophila melanogaster attractant and application thereof. The drosophila melanogaster attractant comprises active ingredients of isoamyl acetate, preferably, the active ingredients of the drosophila melanogaster attractant consist of isoamyl acetate and ethyl palmitate, wherein the weight part ratio of the isoamyl acetate to the ethyl palmitate is 1-10: 1 to 10. The fruit fly attractant disclosed by the invention has the advantages of strong specificity, good attracting effect, long component release duration and the like. The implementation of the invention can reduce the damage degree of the drosophila melanogaster to the fruit production in China, effectively control the population density of pests and has good economic benefit and environmental benefit.)

1. An attractant for Drosophila melanogaster comprises isoamyl acetate as active ingredient.

2. The drosophila melanogaster attractant according to claim 1, wherein: the active ingredients of the compound comprise isoamyl acetate and ethyl palmitate, wherein the weight part ratio of the isoamyl acetate to the ethyl palmitate is 1-10: 1 to 10.

3. The drosophila melanogaster attractant according to claim 2, wherein: the weight part ratio of the isoamyl acetate to the ethyl palmitate is 5-10: 1 to 5.

4. The drosophila melanogaster attractant according to claim 2, wherein: the weight part ratio of the isoamyl acetate to the ethyl palmitate is 1: 1.

5. a drosophila melanogaster trapper comprises a blue insect sticking plate and a sealed and stored drosophila melanogaster trapping core; the drosophila melanogaster lure is the drosophila melanogaster attractant of any one of claims 1-4.

6. The drosophila melanogaster trap according to claim 5, wherein the drosophila melanogaster trap comprises a blue insect sticking plate, the sealed drosophila melanogaster attracting core and a slow release container, the blue insect sticking plate is made of plastic and coated with insect sticking glue, and the insect sticking glue is non-toxic high-viscosity waterproof glue.

7. The drosophila melanogaster trap according to claim 6, wherein the insect sticking plate is provided with a hollow groove or hole, and the shape and size of the hollow groove or hole are the same as those of the slow release container.

8. The drosophila melanogaster trap according to claim 7, wherein the blue mythimna separata panel is rectangular in shape with sides 25 x 20 cm; preferably, the empty groove and the hole are formed in the middle of the pest sticking plate.

9. Use of the drosophila melanogaster attractant of any one of claims 1 to 4 and the drosophila melanogaster trap of any one of claims 5 to 8 in the preparation of a device for trapping drosophila melanogaster or in trapping drosophila melanogaster.

10. The method for using the drosophila melanogaster trap of any one of claims 5 to 8, wherein the drosophila melanogaster trapping core is detached from the sealed package and placed on the blue armyworm plate to trap the drosophila melanogaster at a suitable period for the adult drosophila melanogaster trapping and controlling.

Technical Field

The invention relates to a trapping technology of crop pest drosophila melanogaster, in particular to an attractant for drosophila melanogaster and an application method thereof.

Background

Drosophila melanogaster, Drosophila of the family Drosophilaceae of the order Diptera, is a major pest that has been damaging to fruits in recent years. The pests are distributed in all continents of the world, and Drosophila melanogaster is one of the main species harmful to fruit trees. In recent years, the harm of drosophila melanogaster is on a trend of increasing, and hosts are wide, including more than 60 plants such as grapes, cherries, strawberries, blueberries, blackberries and the like. In addition, the drosophila melanogaster is easy to cause pathogenic bacteria infection in the wounds caused by fruit damage, for example, the drosophila melanogaster plays an important role in the occurrence of grape acid rot, and the secondary damage of the drosophila melanogaster seriously affects the yield and quality of fruits and causes great economic loss, so that the effective prevention and control of the drosophila melanogaster is very urgent.

At present, the control of the drosophila melanogaster is mainly based on chemical control, the chemical control is quick and effective, but the pest period of the drosophila melanogaster is mainly the fruit maturation period of fruit trees, and the pesticide is easy to remain in the fruit, thereby causing unnecessary influence on the production and eating of the fruit trees. The environmental pollution, residue and the defect of the pesticide on animals and pollinating insects are the biggest obstacles for the safe and effective prevention and control of the insects.

Physical control is a main research direction in China in recent years, but the existing physical control effect is still poor. How to provide a black belly drosophila attractant which has good control effect, no pollution to fruits and no pollution to the environment, and the problem to be solved in the control of the black belly drosophila is urgent.

Disclosure of Invention

The invention aims to provide a novel attractant for drosophila melanogaster by utilizing the characteristic of insect tropism to smell aiming at the defects in the field, the attractant is prepared and processed into an attracting core, and the attracting core is combined with an insect sticking plate to improve the continuous trapping capability of the drosophila melanogaster.

The invention aims to provide a drosophila melanogaster attractant which is prepared and processed into an attractant core by taking grape volatile matters, namely isoamyl acetate and ethyl palmitate as raw materials, wherein the active ingredient of the attractant core comprises isoamyl acetate.

The drosophila melanogaster attractant also comprises ethyl palmitate.

In one technical scheme of the invention, the active ingredients of the drosophila melanogaster attractant consist of isoamyl acetate and ethyl palmitate, wherein the weight part ratio of the isoamyl acetate to the ethyl palmitate is 1-10: 1-10.

In a preferred technical scheme of the invention, the ratio of the isoamyl acetate to the ethyl palmitate in parts by weight is 5-10: 1-5.

In another preferred embodiment of the present invention, the ratio of isoamyl acetate to ethyl palmitate is 1:1 by weight.

Another object of the invention is to provide a drosophila melanogaster trap, which comprises a blue armyworm plate and a sealed and preserved drosophila melanogaster trapping core;

preferably, the drosophila melanogaster trap consists of a blue insect sticking plate, a sealed and preserved drosophila melanogaster attracting core and a core attracting container.

When in use, the drosophila melanogaster lure is arranged in a lure container.

The core inducing container is a slow release container.

The slow release container is a slow release bottle or other slow release containers.

The slow release container is preferably a slow release plastic small bottle (the bottle height is 3cm, the bottle body diameter is 1cm, and the bottle mouth diameter is 0.15 cm).

Preferably, the blue insect sticking plate is made of a plastic material and is coated with insect sticking glue, and the insect sticking glue is non-toxic high-viscosity waterproof glue.

Preferably, the pest sticking plate is provided with empty grooves or holes, and the shape and the size of the empty grooves or the holes are the same as those of the slow release container.

Preferably, the blue insect sticking plate is rectangular in shape, and the side length is 25 x 20 cm; preferably, the empty groove and the hole are formed in the middle of the pest sticking plate.

The drosophila melanogaster luring core in the drosophila melanogaster trapper is stored at a low temperature in a sealed manner, and in the suitable period for trapping and controlling adult drosophila melanogaster, the drosophila melanogaster luring core is detached from a sealed package and placed in a luring core container, the luring core container filled with the drosophila melanogaster luring core is placed on the blue pest sticking plate, and the drosophila melanogaster is trapped.

The drosophila melanogaster attractant and the application of the drosophila melanogaster trapper in preparing a device for trapping drosophila melanogaster or trapping drosophila melanogaster also belong to the protection scope of the invention.

In particular to a novel drosophila melanogaster attractant and application thereof. The drosophila melanogaster attractant, the slow-release plastic small bottle and the armyworm backboard are combined into the trap, the drosophila melanogaster attractant in an orchard can be trapped, attractant components in the attracting core can be continuously and effectively released, and the service cycle of the trap is prolonged. The method is realized by the following steps:

(1) Preparing a fruit fly attractant from grape volatile matters according to the mass ratio of isoamyl acetate and ethyl palmitate, and adding the fruit fly attractant into a slow-release plastic bottle to prepare a lure core;

(2) selecting a blue sticky board, filling the lure core into a slow-release plastic small bottle (the bottle height is 3cm, the bottle body diameter is 1cm, and the bottle mouth diameter is 0.15cm), and embedding the lure core on a central hollow groove of the sticky board to form the drosophila melanogaster trapper. The trapper is trapped in the appropriate trapping and controlling period of the drosophila melanogaster imagoes, and the trapper is hung in the orchard at the height close to the height of fruits, so that the quantity of the drosophila melanogaster openings can be effectively reduced.

Adding grape volatile substances into a slow-release plastic vial according to the mass ratio, sealing, and storing at low temperature for later use. The blue sticky board is made of plastic, the insect sticking glue is non-toxic high-viscosity waterproof glue, the blue sticky board is a rectangular board, and the side length is 25 x 20 cm.

The trapping is implemented in the suitable period of the trapping and controlling of the drosophila melanogaster imagoes, and the vineyard imagoes can be effectively trapped and killed.

The drosophila melanogaster attractant disclosed by the invention has the advantages of strong specificity, good attracting effect, long component release duration and the like. The implementation of the invention can reduce the damage degree of the drosophila melanogaster to the fruit production in China, effectively control the population density of pests and has good economic benefit and environmental benefit.

Research results show that the attractant on the lure can be released continuously, the service cycle is prolonged, and a better trapping and killing effect is achieved. The application in the orchard has the advantages of convenient use, safety and no pollution.

Drawings

FIG. 1 trapping effect of different attractant component formulations on Drosophila melanogaster.

FIG. 2 is the effect of the trap made of the insect sticking plates with different colors on trapping the drosophila melanogaster.

FIG. 3 evaluation of the effective period of the trap of Drosophila melanogaster lure.

Detailed Description

In order that the invention may be better understood, the invention will now be further described with reference to specific examples.

The materials and reagents used in the following examples are commercially available unless otherwise specified.

Example 1 screening attractant component formulation for field trapping trials

Respectively mixing grape volatile components isoamyl acetate and ethyl palmitate in a mass ratio of 1: 0. 9:1, 1:9 and 0:1 are respectively prepared into drosophila melanogaster attractants with different concentrations, and the drosophila melanogaster attractants are added into slow-release plastic vials (Beijing Zhongjie Sifang Limited, 3cm in length, 1cm in diameter and 0.15cm in diameter of a bottle mouth), wherein each vial is … … g to prepare the drosophila melanogaster lure core. The five proportions are respectively processing one, processing two, processing three, processing four and processing five.

Placing the drosophila melanogaster attracting core into a central hollow groove of a blue insect sticking plate, wherein the blue insect sticking plate is rectangular and has the side length of 25 x 20 cm; the black drosophila melanogaster trapper is formed, trapping tests are carried out in a trial base in scientific research of a plant protection department of a Chinese academy of agricultural sciences before grapes are ripe for 8 months, and component distribution ratios with good trapping effects under field conditions are screened.

Five drosophila melanogaster lures with different proportions are combined with a blue insect sticking plate to prepare the drosophila melanogaster trapper, and an empty slow-release plastic small bottle and the blue plate are combined to be used as a control, and each treatment is repeated for 3 times. Hanging all traps in the vineyard randomly, wherein the height of each trap is equal to the height of a grape fruit ear, and the distance between each trap and each trap is 5 m. The number of the drosophila melanogaster population 1, 2-4 and 5-7 days after the trapper is hung is counted and investigated.

The results are shown in fig. 1, and the field trapping data shows that the insect trapping amount of the five treatments is higher than that of the blank control. Day 2-4, day 4-7 traps indicated isoamyl acetate: ethyl palmitate was the best trap at 1:1, isoamyl acetate: the trapping effect was also better when the ethyl palmitate was 9:1 and 1: 0. And, when isoamyl acetate: the effect is obviously better than that of isoamyl acetate when the ethyl palmitate is 1: the effect of ethyl palmitate was 9:1 and 1:0, with significant differences.

Example 2 optimal color screening of Pepper plates

The method of example 1 was followed to produce a drosophila melanogaster lure using isoamyl acetate and ethyl palmitate 1:1, which was combined with colored plates of different colors (insect-sticking plates) to produce a drosophila melanogaster trap. The color of the insect sticking plate is selected from white, red, blue, black, green and yellow, and the method is the same as that of the embodiment 1 except that the color of the insect sticking plate is different. Hanging all traps in the vineyard randomly, wherein the height of each trap is equal to the height of a grape fruit ear, and the distance between each trap and each trap is 5 m. The cumulative population of drosophila melanogaster on the color plates was statistically investigated 1, 4, and 7 days after the traps were hung, respectively.

The results are shown in fig. 2, and the test results show that the combination of the lure and the blue color plate has the best trapping effect on the drosophila melanogaster.

Example 3 study of the duration of attracting the Drosophila melanogaster

The method is the same as that of example 1, except that isoamyl acetate and ethyl palmitate are adopted to manufacture the fruit fly luring core, and the luring core and the blue color plate are combined to manufacture the fruit fly trap. This was repeated three times. Hanging all traps in the vineyard randomly, wherein the height of each trap is equal to the height of a grape fruit ear, and the distance between each trap and each trap is 5 m. After the trap is hung, the number of the drosophila melanogaster mouths trapped by the color plates for 1 day, 2-4 days, 5-7 days, 8-10 days, 11-14 days and 15-18 days is respectively investigated.

The result is shown in fig. 3, and the test result shows that the trap still has a good trapping effect on drosophila melanogaster after being hung for 10 days, which indicates that the attractant has long lasting period.