阅读说明：本技术 一种皮肤修复及护理组合物及其制备方法 (Skin repairing and nursing composition and preparation method thereof ) 是由 许帅辉 赵悦华 赵水 常明利 于 2019-11-16 设计创作，主要内容包括：本发明公开了一种皮肤修复及护理组合物及其制备方法,包括层粘连蛋白、纤连蛋白、血清蛋白、小分子活性肽和超氧化物歧化酶和辅助剂。本发明能够提升皮肤整体耐受力,修复受损细胞,加速老化细胞的代谢,改善皮肤微循环,预防色素沉积导致的色斑出现；而层粘连蛋白在周围神经再生过程中既发挥神经营养因子的作用又对神经生长的方向起引导作用,可为皮肤提供营养,保持皮肤水润光泽,增加皮肤弹性,具有良好的修复、吸湿、保湿、抗衰老,组合物可制作为水剂、冻干粉、凝胶剂、液体敷料,对皮肤无刺激,对人体无毒无害,是绿色环保产品。(The invention discloses a skin repairing and nursing composition and a preparation method thereof, wherein the composition comprises laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase and an auxiliary agent. The invention can improve the integral tolerance of the skin, repair damaged cells, accelerate the metabolism of aged cells, improve the microcirculation of the skin and prevent color spots caused by pigment deposition; the laminin plays a role of a neurotrophic factor and plays a role in guiding the growth direction of nerves in the process of regenerating peripheral nerves, can provide nutrition for skin, keeps the skin moist and glossy, increases the skin elasticity, has good repairing, moisture absorption, moisture retention and aging resistance, can be prepared into aqua, freeze-dried powder, gel and liquid dressing, has no stimulation to the skin, is non-toxic and harmless to human bodies, and is a green and environment-friendly product.)

1. The skin repairing and nursing composition is characterized by comprising laminin, fibronectin, serum protein, small molecular active peptide, superoxide dismutase and an auxiliary agent, wherein the specific components are prepared from the following components in parts by weight: 1-17 parts of laminin, 1-14 parts of fibronectin, 1-5 parts of serum protein, 1-3 parts of small molecule active peptide, 1-7 parts of superoxide dismutase and 1-24 parts of an auxiliary agent, and adding a proper amount of sterile physiological saline to make up to 100 parts.

2. The composition for repairing and caring skin according to claim 1, wherein the adjuvant comprises chitosan, polylysine, polyvinyl alcohol, natural antimicrobial agent and glycerin, and the weight ratio of the specific components is: 1-7 parts of chitosan, 1-5 parts of polylysine, 1-3 parts of polyvinyl alcohol, 1-4 parts of natural antibacterial agent and 1-5 parts of glycerol.

3. The skin repair and care composition according to claim 2, wherein the laminin is 17 parts, the fibronectin is 14 parts, the serum protein is 5 parts, the small molecule active peptide is 3 parts, the superoxide dismutase is 7 parts, the chitosan is 7 parts, the polylysine is 5 parts, the polyvinyl alcohol is 3 parts, the natural antibacterial agent is 4 parts, the glycerin is 5 parts, and the sterile physiological saline is 30 parts.

4. The skin repair and care composition according to claim 2, wherein the laminin is 15 parts, the fibronectin is 13 parts, the serum protein is 5 parts, the small molecule active peptide is 3 parts, the superoxide dismutase is 5 parts, the chitosan is 6 parts, the polylysine is 3 parts, the polyvinyl alcohol is 1.5 parts, the natural antibacterial agent is 2.5 parts, the glycerin is 3 parts, and the sterile physiological saline is 43 parts.

5. A skin repair and care composition and a method for preparing the same, comprising the skin repair and care composition according to any one of claims 1 to 4, comprising the steps of:

s1: accurately weighing the required formula amount, fully mixing laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase, chitosan, polylysine, natural antibacterial agent and glycerol in a container, adding a proper amount of sterile normal saline, mixing, and stirring uniformly to obtain a mixed solution for later use;

s2: sterilizing the mixed solution by a filter membrane of 0.22 mu m, and canning in a sterile bottle according to the specification requirement to obtain the laminin skin repair aqueous solution.

6. A skin repair and care composition and a method for preparing the same, comprising the skin repair and care composition according to any one of claims 1 to 4, comprising the steps of:

s1: accurately weighing the required formula amount, fully mixing laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase, chitosan, polylysine and excipient in a container, adding a proper amount of sterile normal saline, mixing, and stirring uniformly to obtain a mixed solution for later use;

s2: and (3) freezing the mixed solution in a refrigerator at the temperature of-20 ℃ for 3 to 8 hours, and then transferring the mixed solution to a vacuum freeze dryer for drying for 5 to 10 hours to obtain dry powder, namely the laminin freeze-dried powder.

7. A skin repair and care composition and a method for preparing the same, comprising the skin repair and care composition according to any one of claims 1 to 4, comprising the steps of:

s1: accurately weighing the required formula amount, fully mixing laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase, chitosan, polylysine, natural antibacterial agent and glycerol in a container, adding a proper amount of sterile normal saline, mixing, and stirring uniformly to obtain a mixed solution for later use;

s2: and (2) freezing the mixed solution in a refrigerator at the temperature of-20 ℃ for 3 to 8 hours, then unfreezing at the temperature of-4 ℃ to 0 ℃, adding 10 parts by weight of carbomer 940, uniformly mixing, and then transferring to a vacuum freeze dryer for drying for 3 to 6 hours to obtain the gel, namely the laminin gel.

8. A skin repair and care composition and a method for preparing the same, comprising the skin repair and care composition according to any one of claims 1 to 4, comprising the steps of:

s1: accurately weighing the required formula amount, fully mixing superoxide dismutase, chitosan, polylysine, polyvinyl alcohol and glycerol in a container, adding a proper amount of sterile normal saline, mixing, and uniformly stirring to obtain a mixed solution for later use;

s2: adding the mixed solution into a batching tank, heating to ensure that the temperature of a finished product liquid in the batching tank is 110-120 ℃ to obtain a finished product liquid, cooling the finished product liquid to 50-60 ℃, adding a natural antibacterial agent, and cooling to normal temperature to obtain a normal-temperature mixed liquid;

s3: adding laminin, fibronectin, serum protein and micromolecular active peptide into the normal-temperature mixed solution to obtain finished solution;

s4: filtering the obtained finished product liquid through a filtering system under the condition of keeping the normal temperature to obtain a dosing liquid;

s5: and filling the prepared mixture by filling equipment, and performing irradiation sterilization to obtain the laminin liquid dressing.

9. The skin rejuvenation and care composition according to claim 6 wherein, according to step S1, said excipient is mannitol.

Technical Field

The invention relates to the technical field of skin care products, in particular to a skin repairing and nursing composition and a preparation method thereof.

Background

The skin aging phenomenon is mainly manifested in two aspects: decline of skin tissue and physiological function of skin. The thickness of the skin changes obviously with the age, the epidermis of the human body is thickest at the age of 20 years and becomes thinner gradually later, the granular layer can shrink to disappear in the old period, the survival period of the spine cells is shortened, the division of the nucleus of the epidermis is increased, so that the melanin is also increased, and the skin color of the old is brownish black mostly. The aged cells adhere to the stratum corneum of the epidermis, which hardens the skin surface and loses its luster. The dermis, which is thickest at age of 30 years, becomes thinner and shrinks with atrophy, has a decrease in subcutaneous fat, and gradually loses skin elasticity and tension due to changes in elastic fibers and collagen fibers, further resulting in skin sagging and wrinkle formation. Meanwhile, sebaceous glands and sweat glands are in failure, the discharge of sweat and sebum is reduced, and the skin gradually loses luster and becomes dry.

On the other hand, in the actual life of people, the phenomena that the skin is wounded, an instrument is damaged, a fresh wound surface, a refractory ulcer wound surface and the like are often encountered, and the skin wound surface is difficult to repair.

Along with the development of economy, the pace of work and life is faster and faster, the mental stress is larger and larger, and in addition, the skin is damaged by a plurality of factors such as bad living habits, bad environments, ultraviolet rays and incorrect skin care, more and more people are troubled by the problem of skin aging.

Disclosure of Invention

The present invention is directed to solving the above problems and providing a skin repair and care composition and a method for preparing the same.

The invention realizes the purpose through the following technical scheme:

a skin repairing and nursing composition comprises laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase and an auxiliary agent, and the specific components are as follows in parts by weight: 1-17 parts of laminin, 1-14 parts of fibronectin, 1-5 parts of serum protein, 1-3 parts of small molecule active peptide, 1-7 parts of superoxide dismutase and 1-24 parts of an auxiliary agent, and adding a proper amount of sterile physiological saline to make up to 100 parts.

The invention is preferable that the auxiliary agent comprises chitosan, polylysine, polyvinyl alcohol, natural antibacterial agent and glycerin, and the weight parts of the specific components are as follows: 1-7 parts of chitosan, 1-5 parts of polylysine, 1-3 parts of polyvinyl alcohol, 1-4 parts of natural antibacterial agent and 1-5 parts of glycerol.

In the present invention, it is preferable that 17 parts of the laminin, 14 parts of the fibronectin, 5 parts of the serum protein, 3 parts of the small molecule active peptide, 7 parts of the superoxide dismutase, 7 parts of the chitosan, 5 parts of the polylysine, 3 parts of the polyvinyl alcohol, 4 parts of the natural antibacterial agent, 5 parts of the glycerol, and 30 parts of the sterile physiological saline be used.

In the present invention, it is preferable that the laminin is 15 parts, the fibronectin is 13 parts, the serum protein is 5 parts, the small molecule active peptide is 3 parts, the superoxide dismutase is 5 parts, the chitosan is 6 parts, the polylysine is 3 parts, the polyvinyl alcohol is 1.5 parts, the natural antibacterial agent is 2.5 parts, the glycerol is 3 parts, and the sterile physiological saline is 43 parts.

A skin repair and care composition and a method of preparing the same, comprising the steps of:

s1: accurately weighing the required formula amount, fully mixing laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase, chitosan, polylysine, natural antibacterial agent and glycerol in a container, adding a proper amount of sterile normal saline, mixing, and stirring uniformly to obtain a mixed solution for later use;

s2: sterilizing the mixed solution by a filter membrane of 0.22 mu m, and canning in a sterile bottle according to the specification requirement to obtain the laminin skin repair aqueous solution.

A skin repair and care composition and a preparation method thereof comprise the following steps:

s1: accurately weighing the required formula amount, fully mixing laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase, chitosan, polylysine and excipient in a container, adding a proper amount of sterile normal saline, mixing, and stirring uniformly to obtain a mixed solution for later use;

s2: and (3) freezing the mixed solution in a refrigerator at the temperature of-20 ℃ for 3 to 8 hours, and then transferring the mixed solution to a vacuum freeze dryer for drying for 5 to 10 hours to obtain dry powder, namely the laminin freeze-dried powder.

A skin repair and care composition and a method of preparing the same, comprising the steps of:

s1: accurately weighing the required formula amount, fully mixing laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase, chitosan, polylysine, natural antibacterial agent and glycerol in a container, adding a proper amount of sterile normal saline, mixing, and stirring uniformly to obtain a mixed solution for later use;

s2: and (2) freezing the mixed solution in a refrigerator at the temperature of-20 ℃ for 3 to 8 hours, then unfreezing at the temperature of-4 ℃ to 0 ℃, adding 10 parts by weight of carbomer 940, uniformly mixing, and then transferring to a vacuum freeze dryer for drying for 3 to 6 hours to obtain the gel, namely the laminin gel.

A skin repair and care composition and a method of preparing the same, comprising the steps of:

s1: accurately weighing the required formula amount, fully mixing superoxide dismutase, chitosan, polylysine, polyvinyl alcohol and glycerol in a container, adding a proper amount of sterile normal saline, mixing, and uniformly stirring to obtain a mixed solution for later use;

s2: adding the mixed solution into a batching tank, heating to ensure that the temperature of a finished product liquid in the batching tank is 110-120 ℃ to obtain a finished product liquid, cooling the finished product liquid to 50-60 ℃, adding a natural antibacterial agent, and cooling to normal temperature to obtain a normal-temperature mixed liquid;

s3: adding laminin, fibronectin, serum protein and micromolecular active peptide into the normal-temperature mixed solution to obtain finished solution;

s4: filtering the obtained finished product liquid through a filtering system under the condition of keeping the normal temperature to obtain a dosing liquid;

s5: and filling the prepared mixture by filling equipment, and performing irradiation sterilization to obtain the laminin liquid dressing.

Preferably, the excipient is mannitol.

The invention has the beneficial effects that:

the invention can improve the integral tolerance of the skin, repair damaged cells, accelerate the metabolism of aged cells, improve the microcirculation of the skin and prevent color spots caused by pigment deposition; the laminin plays a role of a neurotrophic factor and plays a role in guiding the growth direction of nerves in the process of regenerating peripheral nerves, can provide nutrition for skin, keeps the skin moist and glossy, increases the skin elasticity, has good repairing, moisture absorption, moisture retention and aging resistance, can be prepared into aqua, freeze-dried powder, gel and liquid dressing, has no stimulation to the skin, is non-toxic and harmless to human bodies, and is a green and environment-friendly product.

Detailed Description

The invention is further illustrated below:

a skin repairing and nursing composition comprises laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase and an auxiliary agent, and the specific components are as follows in parts by weight: 1-17 parts of laminin, 1-14 parts of fibronectin, 1-5 parts of serum protein, 1-3 parts of small molecule active peptide, 1-7 parts of superoxide dismutase and 1-24 parts of an auxiliary agent, and adding a proper amount of sterile physiological saline to make up to 100 parts.

The auxiliary agent comprises chitosan, polylysine, polyvinyl alcohol, a natural antibacterial agent and glycerol, and the auxiliary agent comprises the following specific components in parts by weight: 1-7 parts of chitosan, 1-5 parts of polylysine, 1-3 parts of polyvinyl alcohol, 1-4 parts of natural antibacterial agent and 1-5 parts of glycerol.

Composition example one:

17 parts of the laminin, 14 parts of the fibronectin, 5 parts of the serum protein, 3 parts of the small molecule active peptide, 7 parts of the superoxide dismutase, 7 parts of the chitosan, 5 parts of the polylysine, 3 parts of the polyvinyl alcohol, 4 parts of the natural antibacterial agent, 5 parts of the glycerol and 30 parts of the sterile physiological saline.

Composition example two:

15 parts of laminin, 13 parts of fibronectin, 5 parts of serum protein, 3 parts of small molecule active peptide, 5 parts of superoxide dismutase, 6 parts of chitosan, 3 parts of polylysine, 1.5 parts of polyvinyl alcohol, 2.5 parts of natural antibacterial agent, 3 parts of glycerol and 43 parts of sterile physiological saline.

Preparation method embodiment one:

a skin repair and care composition and a method of preparing the same, comprising the steps of:

s1: accurately weighing the required formula amount, fully mixing laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase, chitosan, polylysine, natural antibacterial agent and glycerol in a container, adding a proper amount of sterile normal saline, mixing, and stirring uniformly to obtain a mixed solution for later use;

s2: sterilizing the mixed solution by a filter membrane of 0.22 mu m, and canning in a sterile bottle according to the specification requirement to obtain the laminin skin repair aqueous solution.

Preparation method example two:

a skin repair and care composition and a preparation method thereof comprise the following steps:

s1: accurately weighing the required formula amount, fully mixing laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase, chitosan, polylysine and excipient in a container, adding a proper amount of sterile normal saline, mixing, and stirring uniformly to obtain a mixed solution for later use;

s2: and (3) freezing the mixed solution in a refrigerator at the temperature of-20 ℃ for 3 to 8 hours, and then transferring the mixed solution to a vacuum freeze dryer for drying for 5 to 10 hours to obtain dry powder, namely the laminin freeze-dried powder.

The excipient is mannitol.

Preparation method example three:

a skin repair and care composition and a method of preparing the same, comprising the steps of:

s1: accurately weighing the required formula amount, fully mixing laminin, fibronectin, serum protein, small molecule active peptide, superoxide dismutase, chitosan, polylysine, natural antibacterial agent and glycerol in a container, adding a proper amount of sterile normal saline, mixing, and stirring uniformly to obtain a mixed solution for later use;

s2: and (2) freezing the mixed solution in a refrigerator at the temperature of-20 ℃ for 3 to 8 hours, then unfreezing at the temperature of-4 ℃ to 0 ℃, adding 10 parts by weight of carbomer 940, uniformly mixing, and then transferring to a vacuum freeze dryer for drying for 3 to 6 hours to obtain the gel, namely the laminin gel.

Preparation method example four:

a skin repair and care composition and a method of preparing the same, comprising the steps of:

s1: accurately weighing the required formula amount, fully mixing superoxide dismutase, chitosan, polylysine, polyvinyl alcohol and glycerol in a container, adding a proper amount of sterile normal saline, mixing, and uniformly stirring to obtain a mixed solution for later use;

s2: adding the mixed solution into a batching tank, heating to ensure that the temperature of a finished product liquid in the batching tank is 110-120 ℃ to obtain a finished product liquid, cooling the finished product liquid to 50-60 ℃, adding a natural antibacterial agent, and cooling to normal temperature to obtain a normal-temperature mixed liquid;

s3: adding laminin, fibronectin, serum protein and micromolecular active peptide into the normal-temperature mixed solution to obtain finished solution;

s4: filtering the obtained finished product liquid through a filtering system under the condition of keeping the normal temperature to obtain a dosing liquid;

s5: and filling the prepared mixture by filling equipment, and performing irradiation sterilization to obtain the laminin liquid dressing.

In conclusion, the invention can improve the integral tolerance of the skin, repair damaged cells, accelerate the metabolism of aged cells, improve the microcirculation of the skin and prevent color spots caused by pigment deposition; the laminin plays a role of a neurotrophic factor and plays a role in guiding the growth direction of nerves in the process of regenerating peripheral nerves, can provide nutrition for skin, keeps the skin moist and glossy, increases the skin elasticity, has good repairing, moisture absorption, moisture retention and aging resistance, can be prepared into aqua, freeze-dried powder, gel and liquid dressing, has no stimulation to the skin, is non-toxic and harmless to human bodies, and is a green and environment-friendly product.

As will be realized, the invention is capable of other and different modifications and its several details are capable of modifications in various obvious respects, all without departing from the spirit and scope of the present invention.