Multifunctional experimental device for soil microbial community simulation

文档序号:1506662 发布日期:2020-02-07 浏览:36次 中文

阅读说明:本技术 一种土壤微生物群落模拟用多功能实验装置 (Multifunctional experimental device for soil microbial community simulation ) 是由 于赐刚 张振华 刘燕 董姗姗 郭晓平 章嫡妮 卢晓强 马月 胡飞龙 刘立 于 2019-12-07 设计创作,主要内容包括:本发明公开了一种土壤微生物群落模拟用多功能实验装置,包括实验台和支撑腿,实验台上沿长度方向依次设有接水池、水龙头、上端开口的培养箱、电子秤和微生物分离装置,培养箱的侧壁周圈设有加热层,培养箱的一侧面上沿高度方向依次设有不少于一个的取样孔,微生物分离装置包括两侧相互平行布置的立柱,实验台上位于两侧所述立柱之间开有放置槽,放置槽中设有容器,两侧立柱的上端之间插接有放置板,放置板上端中部设有电机,所述电机的输出轴连接有下端延伸至容器底部的搅拌轴,搅拌轴上设有搅拌叶,容器的一侧下部设有取样阀。本发明与现有技术相比的优点在于:功能多样,研究较为方便快捷,提高实验的速度进程。(The invention discloses a multifunctional experimental device for simulating soil microbial community, which comprises an experimental table and supporting legs, wherein a water receiving pool, a water faucet, an incubator with an opening at the upper end, an electronic scale and a microbial separation device are sequentially arranged on the experimental table along the length direction, a heating layer is arranged on the periphery of the side wall of the incubator, at least one sampling hole is sequentially arranged on one side surface of the incubator along the height direction, the microbial separation device comprises stand columns with two sides arranged in parallel, a placing groove is formed between the stand columns on the two sides of the experimental table, a container is arranged in the placing groove, a placing plate is inserted between the upper ends of the stand columns on the two sides, a motor is arranged in the middle of the upper end of the placing plate, an output shaft of the motor is connected with a stirring shaft with the lower end extending to the bottom of the container, stirring. Compared with the prior art, the invention has the advantages that: the function is various, and research is comparatively convenient and fast, improves the speed process of experiment.)

1. The utility model provides a multi-functional experimental apparatus is used in simulation of soil microbiota community, includes laboratory bench (1) and supporting leg (2) that are located laboratory bench (1) bottom surface four corners, its characterized in that: the water receiving pool (3), the incubator (4) with an opening at the upper end, the electronic scale (11) and the microorganism separating device are sequentially arranged on the experiment table (1) along the length direction, a faucet (5) is rotationally arranged between the water receiving pool (3) and the incubator (4) on the experiment table (1), a heating layer (8) is arranged on the periphery of the side wall of the incubator (4), at least one sampling hole (7) is sequentially arranged on one side face of the incubator (4) along the height direction, the microorganism separating device comprises stand columns (12) with two sides arranged in parallel, a placing groove (19) is formed between the stand columns (12) on the experiment table (1) at two sides, a container (17) is arranged in the placing groove (19), a placing plate (13) is inserted between the upper ends of the stand columns (12), and a motor (14) is arranged in the middle of the upper end of the placing plate (13), the output shaft of motor (14) is connected with (mixing) shaft (15) that the lower extreme extends to container (17) bottom, be equipped with stirring leaf (16) on (mixing) shaft (15), one side lower part of container (17) is equipped with sample valve (18).

2. The multifunctional experimental device for soil microbial community simulation of claim 1, wherein: the water outlet end of the water faucet (5) is connected with a shower nozzle (6).

3. The multifunctional experimental device for soil microbial community simulation of claim 1, wherein: the heating wire is uniformly laid in the heating layer (8), and the heat preservation layer (9) is arranged outside the heating layer (8).

4. The multifunctional experimental device for soil microbial community simulation of claim 1, wherein: be equipped with threely on incubator (4) sampling hole (7), and be located the upper and lower portion and the middle part of incubator (4) respectively, incubator (4) have water collector (10) of arranging with the corresponding slope of sampling hole (7) at a side lower extreme rigid coupling that is equipped with sampling hole (7), the higher one end of water collector (10) is located the extreme lower position department the lower extreme of sampling hole (7), and lower one end extends to one side top of water collector (3).

5. The multifunctional experimental device for soil microbial community simulation of claim 1, wherein: the electronic scale (11) is positioned between the incubator (4) and the microorganism separation device.

6. The multifunctional experimental device for soil microbial community simulation of claim 1, wherein: slots are formed in the upper ends of the stand columns (12), insertion rods (20) inserted into the slots are fixedly connected to the lower ends of the two ends of the placing plate (13), and the insertion rods (20) are in clearance fit with the slots.

7. The multifunctional experimental device for soil microbial community simulation of claim 1, wherein: the lateral cabinet (21) and the drawer cabinet (22) which are fixedly connected to the two adjacent supporting legs (2) on the corresponding side are respectively arranged at two ends of the bottom surface of the experiment table (1), and the lower portion of the water receiving tank (3) is located in the lateral cabinet (21).

Technical Field

The invention relates to the technical field of soil microbial experiment, in particular to a multifunctional experimental device for simulating soil microbial community.

Background

Soil microorganisms are a general term for all microorganisms in soil that are invisible or unclear to the naked eye, and shall strictly include bacteria, archaea, fungi, viruses, protozoa, and microscopic algae. The number of individuals is small, generally measured in microns or nanometers, and is usually hundreds of millions to billions in 1 gram of soil, and the types and the number of the individuals vary with the soil environment and the depth of the soil layer. The organic fertilizer can be oxidized, nitrified, ammoniated, nitrogen-fixed, sulfurized and the like in soil to promote the decomposition of soil organic matters and the conversion of nutrients.

To have more understanding to soil microorganism, just need to experiment it, know the environment of soil and to the concrete influence factor of microorganism, this just needs to use experimental apparatus, and present traditional experimental apparatus structure is comparatively simple, can only carry out single experimental processing on it, and it is comparatively troublesome to study, influences the speed process of experiment.

Disclosure of Invention

The invention aims to overcome the technical defects and provide a multifunctional experimental device for simulating the microbial community in the soil, which has various functions, is convenient and quick to research and improves the speed and progress of the experiment.

In order to solve the technical problems, the technical scheme provided by the invention is as follows: a multifunctional experimental device for simulating soil microbial community comprises an experimental table and supporting legs positioned at four corners of the bottom surface of the experimental table, wherein a water receiving pool, an incubator with an opening at the upper end, an electronic scale and a microbial separation device are sequentially arranged on the experimental table along the length direction, a faucet is rotationally arranged between the water receiving pool and the incubator on the experimental table, a heating layer is arranged on the periphery of the side wall of the incubator, at least one sampling hole is sequentially arranged on one side surface of the incubator along the height direction, the microbial separation device comprises stand columns with two sides arranged in parallel, a placing groove is arranged between the stand columns on the two sides of the experimental table, a container is arranged in the placing groove, a placing plate is inserted between the upper ends of the stand columns on the two sides, a motor is arranged in the middle of the upper end of the placing plate, and an output shaft of the motor is connected with a stirring shaft with the lower, the stirring shaft is provided with stirring blades, and the lower part of one side of the container is provided with a sampling valve.

Compared with the prior art, the invention has the advantages that: get the soil sample in same place and place in the incubator, the accessible rotates tap and waters to the soil sample in the incubator, change soil sample's humidity, also can change its temperature for the heating of incubator soil through the zone of heating, can also take out the soil sample of the different degree of depth through the thief hole of co-altitude not, and weigh the soil sample of the same quality through the electronic scale, put in the container, and add the liquid of the same quantity in to the container, and drive the (mixing) shaft at the motor, make the soil suspension under the circumstances of stirring leaf pivoted, and collect the soil suspension sample through the sample valve with the test tube, separate microorganism and soil, research different humidity that can be fine, the temperature, the quantity and the kind of microorganism in the soil degree of depth, the function is various, research is comparatively comprehensive and convenient and fast, the speed process of experiment is improved.

Preferably, tap's play water end is connected with the gondola water faucet shower nozzle, waters for the soil sample in the incubator through the gondola water faucet shower nozzle, and the accuracy of experiment at last is improved to more even of watering.

Preferably, the heater strip has evenly been laid to the inside of zone of heating, the outside of zone of heating is equipped with the heat preservation, reduces thermal scattering and disappearing speed, and is comparatively energy-conserving.

Preferably, be equipped with threely on the incubator the thief hole, and be located the upper and lower part and the middle part of incubator respectively, the incubator is equipped with the water collector that the corresponding slope of thief hole was arranged at a side lower extreme rigid coupling that is equipped with the thief hole, the higher one end of water collector is located the lower extreme of extreme lower position department the lower extreme of thief hole, and lower one end extends to one side top of water collector, and the laboratory can be got the sample of the upper portion of soil respectively, middle part and lower part and study, increases the variety of study, and when watering simultaneously, on water and the water collector can be fallen to from the water that the thief hole flows and small part of soil, and flow to in the water collector, keep the clean health of laboratory bench.

Preferably, the electronic scale is located between the incubator and the microorganism separation device, and a sample taken out from a sampling hole of the incubator can be directly placed on the electronic scale for weighing, so that the electronic scale is convenient and reasonable in layout.

Preferably, both sides the slot has all been opened to the upper end of stand, the equal rigid coupling in both ends lower extreme of placing the board has the inserted bar of pegging graft in the slot, be clearance fit between inserted bar and the slot, the connecting plate is pegged graft through the inserted bar and is connected with both sides stand detachable in the slot, when the container needs to wash, can take off connecting plate and motor, (mixing) shaft area stirring leaf from taking out the container, then can take out the container from the standing groove, makes things convenient for the washing and the change of container.

Preferably, the bottom surface both ends of laboratory bench are equipped with the side of rigid coupling on the adjacent two supporting legs of corresponding side respectively and open cabinet and drawer cabinet, the water receiving pond lower part is located the side and opens the cabinet, and some laboratory utensils can be receive and release to the drawer cabinet, and the laboratory is conveniently taken, saves time, and water receiving pond lower part and connecting pipe simultaneously, tap's connecting pipe all is located the side and opens the cabinet in addition, and the outward appearance is comparatively pleasing to the eye.

Drawings

FIG. 1 is a schematic structural diagram of a multifunctional experimental apparatus for soil microbial community simulation according to the present invention.

As shown in the figure: 1. experiment table, 2, supporting leg, 3, water receiving tank, 4, incubator, 5, tap, 6, gondola water faucet shower nozzle, 7, thief hole, 8, zone of heating, 9, heat preservation, 10, water collector, 11, electronic scale, 12, stand, 13, place the board, 14, motor, 15, (mixing) shaft, 16, stirring leaf, 17, container, 18, sample valve, 19, standing groove, 20, inserted bar, 21, side switch cabinet, 22, drawer cabinet.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

A multifunctional experimental device for simulating soil microbial community comprises an experimental table 1 and supporting legs 2 located at four corners of the bottom surface of the experimental table 1, wherein a water receiving pool 3, an incubator 4 with an opening at the upper end, an electronic scale 11 and a microbial separation device are sequentially arranged on the experimental table 1 along the length direction, a faucet 5 is rotationally arranged on the experimental table 1 between the water receiving pool 3 and the incubator 4, the rotating faucet 5 adopts the existing rotatable faucet, meanwhile, a drain pipe at the lower end of the water receiving pool 3 is communicated with a pre-reserved drainage channel, a heating layer 8 is arranged on the periphery of the side wall of the incubator 4, at least one sampling hole 7 is sequentially arranged on one side surface of the incubator 4 along the height direction, the microbial separation device comprises stand columns 12 with two sides arranged in parallel, a placing groove 19 is formed between the stand columns 12 on the experimental table 1 at two sides, be equipped with container 17 in the standing groove 19, container 17 is the transparent glass container of the area scale for the experiment, both sides it has place board 13 to peg graft between the upper end of stand 12, it is equipped with motor 14 to place board 13 upper end middle part, and motor 14 passes through the screw rigid coupling and is placing the middle part of board 13, and the power input end of motor 14 passes through the plug and is connected the power supply with external socket, there is (mixing) shaft 15 that the lower extreme extends to container 17 bottom through the coupling joint on the output shaft of motor 14, be equipped with stirring leaf 16 on the (mixing) shaft 15, one side lower part of container 17 is equipped with sample valve 18.

The water outlet end of the water faucet 5 is connected with a shower nozzle 6, the lower end of the water faucet 5 is provided with a water inlet pipe and is connected with a reserved water supply pipe, and the water inlet pipe is positioned inside the side cabinet 21.

The heater strip has evenly been laid to zone of heating 8's inside, zone of heating 8's outside is equipped with heat preservation 9, zone of heating 8 is the cavity that forms between zone of heating 8's the lateral wall and incubator 4's the lateral wall, through the fixed heater strip of little pipe strap on the cavity inner wall, the heater strip power supply end passes through the plug and is connected the power supply with external saddle, install the temperature controller on the connecting circuit of heater strip simultaneously, can be so that zone of heating 8 can keep constant temperature, zone of heating 8's outer wall rubberizing is stained with one deck heat preservation 9 in addition, thief hole 7 runs through zone of heating 8, the outer wall of heat preservation 9 and incubator 4, the aperture of thief hole 7 should not be too big, avoid soil to follow a large amount of overflows of thief hole, incubator 4 adopts the.

Be equipped with threely on incubator 4 sampling hole 7, and be located incubator 4's upper and lower portion and middle part respectively, incubator 4 has water collector 10 of arranging with the corresponding slope of sampling hole 7 at a side lower extreme rigid coupling that is equipped with sampling hole 7, the higher one end of water collector 10 runs through the welding of heat preservation 9 on the outer wall of zone of heating 8 that is located lowest position department below sampling hole 7, and lower one end extends to one side top of water collector 3.

The electronic scale 11 is located between the incubator 4 and the microorganism separation apparatus.

Slots are formed in the upper ends of the upright columns 12, inserting rods 20 inserted into the slots are fixedly connected to the lower ends of the two ends of the placing plate 13, and the inserting rods 20 and the slots are in clearance fit.

The two ends of the bottom surface of the experiment table 1 are respectively provided with a side cabinet 21 and a drawer cabinet 22 which are fixedly connected to two adjacent supporting legs 2 on the corresponding sides, the lower part of the water receiving tank 3 is positioned in the side cabinet 21, and the side cabinet 21 is a cabinet with a side door which is often seen by people.

When the invention is implemented concretely, a soil sample in the same place is taken and placed in an incubator, a water faucet can be rotated to open a water valve, water is uniformly watered to the soil sample in the incubator through a shower nozzle to change the humidity of the soil sample, a water receiving disc can receive water and less soil flowing out of three sampling holes and flows into a water receiving pool to be discharged through a drain pipe at the lower end, the cleanness of an experiment table is kept, a heating wire in a heating layer can be electrified, the temperature required to be heated is adjusted through a set temperature controller to heat the soil in the incubator to change the temperature of the soil, samples with different depths at the upper part, the middle part and the lower part of the soil sample can be taken out through the three sampling holes with different heights, the soil sample with the same mass is weighed through an electronic scale and placed in a container, the same amount of liquid is added into the container, and a stirring shaft is driven, Make soil suspension under the circumstances that the stirring leaf rotated to collect the soil suspension sample through the sample valve with the test tube, then put and diluting on the culture dish, make the bacterial colony of developing in the culture dish disperse well, these divided microbial cell grow into dispersed bacterial colony on the nutrition agar plate, convert the quantity of microorganism in the soil of unit weight according to the bacterial colony number, can observe the kind of microorganism according to the microscope simultaneously. The quantity and the kind of microorganism in different humidity, temperature, the soil degree of depth in research that can be fine, the function is various, and research is comparatively comprehensive and convenient and fast, improves the speed process of experiment.

The present invention and its embodiments have been described above, and the description is not intended to be limiting, and the drawings are only one embodiment of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention as defined by the appended claims.

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