Fixing device and experiment positioning device
阅读说明：本技术 固定装置及实验定位装置 (Fixing device and experiment positioning device ) 是由 陈兵 顾建文 时全星 罗灵 聂闯 赵智平 吴清新 于 2019-11-22 设计创作，主要内容包括：本发明申请公开了一种固定装置及实验定位装置,包括固定面板,承载用于实验的小鼠；定位结构,对所述小鼠进行位置固定,设置在所述固定面板上；所述固定面板被设定为,包括冲击孔,所述冲击孔对称设置在以所述固定面板的结构中心为交点的两条呈90°夹角的第一轴线上,所述冲击孔距离所述固定面板的结构中心的距离相等；所述定位结构设置在与所述第一轴线呈45°夹角的第二轴线上和/或所述第一轴线上。本发明申请解决了以解决由于常规的小鼠固定装置仅对小鼠的四肢进行捆绑,且躯体暴露在爆炸冲击波中,导致的冲击波打击部位不准确,同时实验结果受多种因素干扰,造成的实验结果精确度低的技术问题。(The invention discloses a fixing device and an experiment positioning device, which comprise a fixing panel, a positioning plate and a positioning plate, wherein the fixing panel is used for bearing a mouse for an experiment; the positioning structure is used for fixing the position of the mouse and is arranged on the fixing panel; the fixed panel is set to comprise impact holes, the impact holes are symmetrically arranged on two first axes which take the structural center of the fixed panel as an intersection point and form an included angle of 90 degrees, and the distances from the impact holes to the structural center of the fixed panel are equal; the positioning structure is arranged on a second axis which forms an included angle of 45 degrees with the first axis and/or the first axis. The invention solves the technical problems that the impact wave striking part is inaccurate and the experimental result is interfered by various factors, so that the accuracy of the experimental result is low, because the conventional mouse fixing device only binds the limbs of the mouse and the body is exposed in the explosion impact wave.)
1. A fixation device, comprising:
a fixing panel (1) for carrying a mouse for experiment;
a positioning structure (2) for fixing the position of the mouse and arranged on the fixing panel (1);
the fixed panel (1) is set to comprise impact holes (101), the impact holes (101) are symmetrically arranged on two first axes (A) which take the structural center of the fixed panel (1) as an intersection point and form an included angle of 90 degrees, and the distances from the impact holes (101) to the structural center of the fixed panel (1) are equal;
the positioning structure (2) is arranged on a second axis (B) forming an included angle of 45 degrees with the first axis (A) and/or the first axis (A).
2. The fixing device according to claim 1, characterized in that it comprises a barrier (3) connected to the positioning structure (2) and/or to the fixing panel (1) to protect the mouse from axonal damage during the experiment.
3. A fixation device according to claim 1, wherein the positioning structure (2) comprises a first positioning member (201) for positionally fixing the mouse body, the first positioning member (201) being arranged on the first axis (a).
4. A fixing device according to claim 3, characterized in that the positioning structure (2) comprises a second positioning member (202) for fixing the mouse head, and the second positioning member (202) correspondingly fixes the mouse head at the impact hole (101).
5. A fixation device according to claim 4, characterized in that the second positioning element (202) comprises at least 4 bolts (203), said bolts (203) penetrating the fixation panel (1) being connected with a fixation wire (4) wound around the head of the mouse.
6. A fixation device according to claim 5, wherein a plurality of said bolts (203) are arranged equidistantly on said second axis (B).
7. A fixture according to claim 5, characterised in that between the bolts (203) there is a stop for further fixing the mouse head.
8. A fixation device according to any one of claims 4 to 5, characterized by comprising a third positioning element (204) located in the structural centre of the fixation panel (1), said third positioning element (204) being connected to a fixation wire (4) that is wound around the teeth of the mouse.
9. The utility model provides an experiment positioner, includes fixing device (5) and support (6), its characterized in that, support (6) are height-adjustable support.
10. Experiment positioning device according to claim 9, characterized in that it comprises a shielding member (7), said shielding member (7) being arranged around the outside of the fixture (5), said shielding member (7) being provided with structural holes (701) corresponding to the impingement holes of the fixture (5).
Technical Field
The invention relates to the field of biomedical experimental instruments, in particular to a fixing device and an experimental positioning device.
Background
The mouse craniocerebral injury model is an important tool for researching a brain trauma injury mechanism and evaluating an intervention effect, wherein the explosive shock wave craniocerebral injury animal model is one of common brain trauma models. When the mouse explodes the craniocerebral injury, the shock wave can be ensured to accurately strike the craniocerebral position of the mouse, and the left and right or front and back large-amplitude swing of the head of the mouse is avoided. There is therefore a need for a device that secures the mouse head and exposes the head to shock waves.
However, the existing explosive shock wave craniocerebral injury mouse fixing device has some defects: firstly, the operation is complicated, the modeling efficiency is low, four limbs of a mouse need to be bound, the teeth of the mouse need to be fixed so as to achieve the purpose of fixing the head of the mouse, and each explosion shock wave can only damage one mouse, so that a long time is needed; secondly, some mouse fixing devices expose the whole body of the mouse in the explosion shock wave, so that the craniocerebral injury of the mouse is caused, and other parts of the mouse, such as the abdomen, the chest, the eyes and the like, are also damaged to a certain extent, which brings a plurality of interference factors to the research of the craniocerebral injury of the mouse. Therefore, the novel fixing device for craniocerebral injury caused by the experimental mouse explosion shock wave is designed, and has important significance for experimental research of craniocerebral injury
Disclosure of Invention
The invention mainly aims to provide a fixing device to solve the technical problems that the impact part of shock waves is inaccurate and experimental results are interfered by various factors, so that the accuracy of the experimental results is low, wherein the conventional mouse fixing device only binds the limbs of a mouse and exposes the body of the mouse to the explosion shock waves.
Meanwhile, the invention can simultaneously carry out experiments on a plurality of mice through the arrangement of the structure, thereby improving the efficiency of the experiments, ensuring that the impact force of the plurality of mice in the experiment process is the same through the setting of the symmetrical structure, and further improving the accuracy of the experiment data.
In order to achieve the above object, according to one aspect of the present application, a fixing device is provided.
The fixing device according to the present application comprises:
a fixing panel for bearing a mouse for experiment;
the positioning structure is used for fixing the position of the mouse and is arranged on the fixing panel;
the fixed panel is set to comprise impact holes, the impact holes are symmetrically arranged on two first axes which take the structural center of the fixed panel as an intersection point and form an included angle of 90 degrees, and the distances from the impact holes to the structural center of the fixed panel are equal;
the positioning structure is arranged on a second axis which forms an included angle of 45 degrees with the first axis and/or the first axis.
Further, the isolation layer is used for preventing the mouse from being damaged by the axle cable in the experimental process and is connected with the positioning structure and/or the fixing panel.
Further, the positioning structure comprises a first positioning piece for fixing the position of the mouse body, and the first positioning piece is arranged on the first axis.
Furthermore, the positioning structure comprises a second positioning part for fixing the head of the mouse, and the head of the mouse is correspondingly fixed at the impact hole by the second positioning part.
Furthermore, the second positioning piece comprises at least 4 bolts, and the bolts penetrate through the fixing panel and are connected with a fixing line wound around the head of the mouse.
Further, a plurality of the bolts are arranged on the second axis at equal intervals.
Furthermore, a baffle plate for further fixing the head of the mouse is arranged between the bolts.
Furthermore, the mouse fixing device comprises a third positioning piece positioned at the structural center of the fixing panel, and the third positioning piece is connected with a fixing line wound around teeth of the mouse.
In order to achieve the above object, according to another aspect of the present application, an experimental positioning device is provided.
The experimental positioning device according to the invention comprises: the support is a height-adjustable support.
Furthermore, the shielding component is arranged outside the fixing device in a surrounding mode, and structural holes are formed in the shielding component corresponding to the impact holes of the fixing device.
Further, the shielding component and the fixing device are designed integrally.
In the embodiment of the invention, a new fixing device is set, the head of the mouse is limited on the impact hole of the fixing panel through the fixing panel and the setting of the impact hole and the positioning structure of the fixing panel, the exposure of the body of the mouse is avoided, and the mouse is hit at a specified position through the impact hole on the fixing surface, so that the fixing reliability of the mouse in the experiment process is improved, the technical effect of multiple interference factors in the experiment process is reduced, and the technical problems that the hitting position of the impact wave is inaccurate, the experiment result is interfered by multiple factors and the accuracy of the experiment result is low due to the fact that the conventional mouse fixing device only binds the limbs of the mouse and the body is exposed in the explosion impact wave are solved.
Meanwhile, the fixed panel and the impact holes thereof and the positioning structure are distributed in an axisymmetric structure, so that the fixing device can fix a plurality of mice simultaneously, and the positions of the mice are distributed relatively close to the positions of the explosion points, therefore, the stress is also the same, the experiment efficiency is improved, and the experiment quality is further improved.
The frame is a height-adjustable support.
Furthermore, the shielding component is arranged outside the fixing device in a surrounding mode, and structural holes are formed in the shielding component corresponding to the impact holes of the fixing device.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, serve to provide a further understanding of the invention and to make further features, objects, and advantages of the invention apparent. The drawings and their description illustrate exemplary embodiments of the invention and do not limit it inappropriately. In the drawings:
FIG. 1 is a schematic view showing the overall structure of a fixing device according to an embodiment of the present invention;
FIG. 2 is a schematic view of the position of the spacer of the fastening device according to an embodiment of the present invention;
FIG. 3 is a schematic front view of an experimental positioning device according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a back side structure of an experimental positioning device according to an embodiment of the present invention;
FIG. 5 is a schematic side view of an experimental positioning device according to an embodiment of the present invention;
FIG. 6 is a schematic side view B of an experimental positioning device according to an embodiment of the present invention.
Reference numerals
1: fixing the panel; 101: an impingement hole; 2: a positioning structure; 201: a first positioning member; 202: a second positioning member; 203: a bolt; 204: a third positioning member; 3: an interlayer; 301: a first barrier layer; 302: a second barrier layer; 4: fixing the wire; 5: a fixing device; 6: a support; 7: a shielding member; 701: structural holes; a: a first axis; b: a second axis.
Detailed Description
In order to make the person skilled in the art better understand the application scheme of the present invention, the technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any inventive step based on the embodiments in the present application, shall fall within the scope of protection of the present application.
It should be noted that the terms "first," "second," and the like in the description and claims of the present application and in the above-described drawings are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged under appropriate circumstances such that embodiments of the present application are described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
In the present application, the terms "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings. These terms are used primarily to better describe the present application and its embodiments, and are not intended to limit the indicated devices, elements or components to a particular orientation or to be constructed and operated in a particular orientation.
Moreover, some of the above terms may be used to indicate other meanings besides the orientation or positional relationship, for example, the term "on" may also be used to indicate some kind of attachment or connection relationship in some cases. The specific meanings of these terms in the present application can be understood by those of ordinary skill in the art as appropriate.
Furthermore, the terms "mounted," "disposed," "provided," "connected," and "sleeved" are to be construed broadly. For example, it may be a fixed connection, a removable connection, or a unitary construction; can be a mechanical connection, or an electrical connection; may be directly connected, or indirectly connected through intervening media, or may be in internal communication between two devices, elements or components. The specific meanings of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the drawings.
As shown in fig. 1, the present application relates to a fixing device, which includes a fixing panel 1 and a positioning structure 2, wherein the fixing panel 1 carries a mouse for experiment, the positioning structure 2 is disposed on the fixing panel 1, and the positioning structure 2 fixes the position of the mouse; the fixed panel 1 time is set to comprise impact holes 101, the impact holes 101 are symmetrically arranged on two first axes A which take the structural center of the fixed panel 1 as a focus and form an included angle of 90 degrees, and the distances between the impact holes 101 and the structural center of the fixed panel 1 are equivalent; the positioning structure 2 is arranged on a second axis B forming an included angle of 45 degrees with the first axis A and/or the first axis A; specifically, the second axis B and the first axis a form an included angle of 45 degrees, that is, the second axis B is a bisector of an included angle formed by the two first axes a.
In the preferred embodiment of the present invention, two first axes a are included, the first axes a are respectively arranged along a horizontal direction and a vertical direction, the fixed panel 1 includes 4 impact holes 101, and the impact holes 101 are symmetrically arranged around the structural center of the fixed panel 1 and are arranged on the first axes a at equal distances from the structural center. Further preferably, the impact holes 101 are round openings with a diameter of 1 cm, so that brains of experimental mice are exposed to the shock waves, the shock waves received by each impact hole 101 are the same in size, and only the heads of the mice are exposed to the shock waves.
In a preferred embodiment of the present application, the positioning structure includes a first positioning element 201, a second positioning element 202, and a third positioning element 204, the first positioning element 201 is disposed on the first axis a, and is configured to fix a position of the body of the mouse, and preferably, the first positioning element 201 is a hook-and-loop fastener; utilize the thread gluing to carry out the fixed of mouse body, can simply operate fast, reduce the fixed degree of difficulty of experiment mouse, improve experimental efficiency, it is specific, the thread gluing is fixed the belly of mouse. The second fixing part 202 is used for fixing the position of the head of the mouse corresponding to the impact hole 101 of the fixing plate surface 1, so that the head of the mouse can be hit through the impact hole 101 in the experiment process, and the success rate of the experiment is improved. Specifically, the second fixing member 202 at least includes 4 bolts 203, and the bolts 203 are equidistantly arranged on the second axis; the bolt 203 penetrates through the fixing panel 1, preferably, the bolt penetrating through the fixing panel 1 is fixed in position, and the bolt is prevented from moving in a through hole of the fixing panel 1; and winding a fixing wire 4 on 4 bolts 203, wherein the fixing wire 4 is wound on the head of the mouse and the bolts 203 at intervals, and finally, the heads of the mice are fixed by taking the bolts 203 as positioning points. In a further preferred embodiment of the present application, a third positioning member 204 is included, the third positioning member 204 is disposed at the structural center of the fixing panel 1, and the third positioning member 204 fixes the teeth of the mouse together with the fixing wire 4, so as to further fix the head of the mouse.
In a further preferred embodiment of the present application, a baffle plate for further fixing the head of the mouse is disposed between the plurality of bolts 203 of the second positioning member 202, as shown in fig. 1, in the preferred embodiment of the present application, the baffle plate disposed between the bolts 203 at least includes two baffle plates, which are respectively disposed on two sides of the impact hole 101, for fixing the position of the head of the mouse, so as to avoid the occurrence of the situation that the final experimental effect is affected due to inaccurate experimental data caused by the change of the position of the head of the mouse during the experimental process.
In a further preferred embodiment of the present application, the device comprises a barrier layer 3 connected to the positioning structure 2 and/or the fixing plate 1 for preventing the mouse from being damaged by axons during the experiment; as shown in fig. 2, the partition layer 3 includes a first partition layer 301 disposed on the fixed panel 1 and a second partition layer 302 sleeved on the second positioning member 202 and/or the third positioning member 204, the first partition layer 301 is disposed between the mouse and the fixed panel 1, so as to prevent the mouse from directly contacting the fixed panel 1, which may cause discomfort to the body of the mouse due to too low or too high temperature of the fixed panel 1; the first interlayer 301 and the second interlayer 302 are both made of a cushion layer made of a flexible material, specifically, the flexible material may be felt cloth or other materials with good heat insulation performance and poor temperature conduction performance. In the preferred embodiment of the present invention, the second interlayer 302 is a cushion layer made of a flexible material with a circular outer contour; specifically, the second interlayer 302 is sleeved on the bolt 203 of the second positioning element 202, and the position of the second interlayer 302 is fixed through a nut, so that the second interlayer 302 is in contact with the head of a mouse, and the damage to an axle cable caused by the extrusion of a first fixing component when the position of the mouse is fixed is avoided. The height of gasket can be adjusted through the nut on the bolt, makes the not mouse head of equidimension can both laminate completely, firmly fixes the head to the one side of mouse contact is flexible material, guarantees that the mouse can normally breathe and prevent to hang down or too high discomfort that brings for the mouse because of the temperature with the one side of mouse contact.
In a further preferred embodiment of the application of the invention, 4 impact holes with the diameter of 1 cm are punched at a certain distance from the center of the panel, four small holes are punched at the center of the panel and other positions, screws are installed, a first interlayer made of soft material is adhered to the panel, and thread gluing is installed at the position 2 cm right below the four round holes; the second interlayer 302 comprises a round sheet made of steel sheet and having a diameter of 7 cm, a layer of soft material is adhered on the round sheet, and four small holes are drilled in the center of the round sheet and at four positions 2.5 cm away from the center and correspond to the four small holes on the panel.
In order to achieve the above object, according to another aspect of the present application, an experimental positioning device is provided.
As shown in fig. 3 and 4, the experimental positioning device according to the present invention includes: the device comprises a
further, the shielding device comprises a
In the preferred embodiment of the present invention, as shown in fig. 5, the
In other embodiments, the shielding
In the actual operation process, a mouse lies on the back on a panel, whether the cranium of the experimental mouse is exposed outside is observed firstly, then the abdomen of the mouse is fixed by a first positioning piece, namely a sticky buckle in the preferred embodiment, after the abdomen of the mouse is fixed, a fixing line is used for surrounding four different bolts for two circles, the brain of the mouse is fixed by the surrounding fixing line, and the whole body and the head of the mouse are fixed firmly. The second interlayer is mounted by adjusting the height of the nut on the bolt, and the second interlayer is tightly attached to the head of the mouse, so that the mouse is prevented from being damaged by the axle cable instantly when being struck by shock waves. The first interlayer on the fixed panel contacting the mouse body and the second interlayer connected with the bolt are both paved with a layer of soft material, so that the pain of the mouse and the discomfort of the mouse caused by too low or too high temperature of the panel in the experimental process can not be caused. The fixing panel in the fixing device of the experimental positioning device can fix four experimental mice at a time. After the mouse is fixed, the fixing panel is installed on the shielding box, and the fixing panel is firmly fixed with the iron buckles on the two sides of the shielding box. And the whole device is firmly fixed on the horizontal ground at the bottom of the fixing frame by using screws, so that the whole device is prevented from generating certain displacement due to overlarge shock waves.
In the embodiment of the invention, a new fixing device is set, the head of the mouse is limited on the impact hole of the fixing panel through the fixing panel and the setting of the impact hole and the positioning structure of the fixing panel, the exposure of the body of the mouse is avoided, and the mouse is hit at a specified position through the impact hole on the fixing surface, so that the fixing reliability of the mouse in the experiment process is improved, the technical effect of multiple interference factors in the experiment process is reduced, and the technical problems that the hitting position of the impact wave is inaccurate, the experiment result is interfered by multiple factors and the accuracy of the experiment result is low due to the fact that the conventional mouse fixing device only binds the limbs of the mouse and the body is exposed in the explosion impact wave are solved.
Meanwhile, the fixed panel and the impact holes thereof and the positioning structure are distributed in an axisymmetric structure, so that the fixing device can fix a plurality of mice simultaneously, and the positions of the mice are distributed relatively close to the positions of the explosion points, therefore, the stress is also the same, the experiment efficiency is improved, and the experiment quality is further improved.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
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