阅读说明：本技术 仔猪用饲料添加剂 (Feed additive for piglets ) 是由 陈春林 翟少钦 闫志强 付文贵 朱买勋 唐红梅 郑刚 于 2019-12-11 设计创作，主要内容包括：本发明属于含有来自被子植物亚门的植物的药物制剂技术领域,具体涉及一种仔猪用饲料添加剂。仔猪用饲料添加剂,其特征在于,由杜仲叶、松针、女贞子和菟丝子依次经烘干、粉碎、过筛、灭菌,然后经微生物菌剂发酵而得,所述微生物菌剂包括地衣芽胞杆菌、丁酸梭菌和枯草芽孢杆菌。本发明的饲料添加剂对于仔猪消化道疾病的疗效快,用量少,成本低。(The invention belongs to the technical field of medicinal preparations containing plants from angiosperm subgenus, and particularly relates to a piglet feed additive. The feed additive for the piglets is characterized by being prepared by drying, crushing, sieving and sterilizing eucommia leaves, pine needles, glossy privet fruits and semen cuscutae in sequence and then fermenting by using a microbial agent, wherein the microbial agent comprises bacillus licheniformis, clostridium butyricum and bacillus subtilis. The feed additive has the advantages of quick curative effect, small dosage and low cost on piglet digestive tract diseases.)

1. The feed additive for the piglets is characterized by being prepared by drying, crushing, sieving and sterilizing eucommia leaves, pine needles, glossy privet fruits and semen cuscutae in sequence and then fermenting by using a microbial agent, wherein the microbial agent comprises bacillus licheniformis, clostridium butyricum and bacillus subtilis.

2. The piglet feed additive according to claim 1, wherein the mixture ratio of the eucommia ulmoides leaves, the pine needles, the glossy privet fruits and the semen cuscutae in parts by mass is as follows: 30-50 parts of eucommia leaves, 20-30 parts of pine needles, 10-20 parts of glossy privet fruits and 5-10 parts of semen cuscutae.

3. The piglet feed additive according to claim 2, wherein the mixture ratio of the eucommia ulmoides leaves, the pine needles, the glossy privet fruits and the semen cuscutae in parts by mass is as follows: 50 parts of eucommia leaves, 30 parts of pine needles, 15 parts of glossy privet fruits and 5 parts of south dodder seeds.

4. The piglet feed additive according to any one of claims 1 to 3, wherein the drying is specifically drying at 40-60 ℃ for 24-36 h.

5. The piglet feed additive according to any one of claims 1 to 4, wherein the sieving is specifically 80-100 mesh sieving.

6. The piglet feed additive according to any one of claims 1 to 5, wherein the sterilization is specifically performed at 115-123 ℃ for 20-30min and then cooled to 35-40 ℃.

7. The piglet feed additive according to any one of claims 1 to 6, wherein the total number of bacteria in the microbial agent is 0.5X 10⁸-1.0×10⁸cfu/ml。

8. The piglet feed additive according to any one of claims 1 to 7, wherein the microbial inoculant comprises Bacillus licheniformis, Clostridium butyricum and Bacillus subtilis in a volume ratio of 0.8-1.2:0.8-1.2: 0.8-1.2.

9. The piglet feed additive according to any one of claims 1 to 8, wherein the fermentation is specifically at 30-37 ℃ for 48-72 h.

10. The piglet feed additive according to any one of claims 1 to 9, wherein the piglet feed additive is prepared by drying 30 to 50 parts by mass of eucommia ulmoides leaves, 20 to 30 parts by mass of pine needles, 10 to 20 parts by mass of glossy privet fruits and 5 to 10 parts by mass of semen cuscutae at 40 to 60 ℃ for 24 to 36 hours, crushing, sieving with a 80 to 100-mesh sieve, sterilizing at 123 ℃ for 20 to 30 minutes, cooling to 35 to 40 ℃, and fermenting with a microbial agent at 30 to 37 ℃ for 48 to 72 hours, wherein the microbial agent comprises bacillus licheniformis, clostridium butyricum and bacillus subtilis, and the total bacterial number in the microbial agent is 0.5 x 10⁸-1.0×10⁸cfu/ml, wherein the volume ratio of the bacillus licheniformis to the clostridium butyricum to the bacillus subtilis in the microbial agent is 0.8-1.2:0.8-1.2: 0.8-1.2.

Technical Field

The invention belongs to the technical field of medicinal preparations containing plants from angiosperm subgenus, and particularly relates to a piglet feed additive.

Background

The piglet digestive tract diseases seriously restrict the development of healthy breeding of live pigs (the epidemic situation and prevention and control measures of systemic diseases of pigs, rejuvenation, guidance of veterinarians, 11 th year 2017, 1 st line of abstract page 6, and 30 th year 2017, 06 months 2017). According to statistics, the incidence rate of digestive tract diseases of piglets reaches 50.47%, the mortality rate of bacterial diseases reaches more than 50%, and the mortality rate of viral diarrhea reaches 100% every year. In recent 30 years, the detection, prevention and control of piglet digestive tract diseases in China have obvious breakthrough, but the effect is unsatisfactory, and livestock and poultry epidemic diseases are continuously outbreak and new diseases are continuously appeared. In the period, a large amount of antibiotics are used for disease resistance, growth promotion and disease prevention and control in the live pig breeding process. The long-term use of the antibiotics in large quantities causes drug resistance and drug residue of bacteria, which not only harms the healthy breeding of live pigs, but also causes pollution to the ecological environment. In 2017, the department of agricultural rural areas issues a notice of action plans for inhibiting animal-derived bacteria drug resistance in China (2017 + 2020), and provides important animal antibacterial risk assessment and early warning prompts to be enhanced, so that the security risk assessment strength is increased, a time schedule and a technical route map are definitely assessed, the elimination of potential risk varieties is accelerated, and the gradual exit of antibacterial drugs for growth promotion is promoted. Therefore, the search for a novel green, ecological and efficient preparation for preventing and controlling piglet digestive tract diseases and a matched prevention and control technology thereof is the fundamental way for solving the problem.

The veterinary drug preparation plays an important role in preventing and controlling piglet digestive tract diseases. The traditional Chinese medicine has the characteristics of greenness, health, safety and no toxicity, contains a plurality of effective active ingredients and abundant nutrient substances, also contains a large amount of essential amino acids, trace elements and the like, can fully play the effects of preventing, treating and protecting health in the live pig breeding, efficiently prevents and controls the digestive tract diseases of piglets, and ensures the green, healthy, ecological and sustainable development of the live pigs. The ministry of agricultural rural areas issues guidance opinions on promoting the health development of veterinary drug industry (Nongjiafa [2016] 15) in the ministry of agricultural rural areas, and clearly indicates that the development of the veterinary drug industry is to be accelerated. In 4 months in 2018, the national institutes of health and medicine made and published 'the first catalog of ancient classic famous-square' (catalog) ', in 6 months in 2018, the national institutes of health and medicine issued' simplified registration approval management regulations of ancient classic famous-square compound Chinese medicine '(management regulations)', in 6 months in 2018, and the veterinary bureau in the agricultural rural area drafted 'Chinese veterinary medicine, natural medicine classification and registration data requirements (revised draft)', and the guidance of the policies promotes the development of the novel Chinese veterinary medicine industry. Therefore, the development of a novel traditional Chinese veterinary medicine preparation is enhanced, the popularization of a prevention and control matching technology is promoted, the tissue structure damage and the healthy development of the early piglet digestive tract caused by the use of chemical medicines are protected, the healthy reproduction of intestinal flora is promoted, the nonspecific immunity of piglets is improved, the growth and the development of the piglets can be promoted, and the development of the live pig breeding industry is promoted.

At present, people carry out related research on traditional Chinese medicines for preventing and treating piglet digestive tract diseases. However, the medicines have slow curative effect, large dosage and high cost on piglet digestive tract diseases. The development of traditional Chinese medicine fermentation products can reduce the consumption of traditional Chinese medicine resources, expand the application scope of traditional Chinese medicines and promote the significant reduction and synergism of the traditional Chinese medicine feed additive for animals.

Disclosure of Invention

In view of the above, the invention aims to provide a piglet feed additive which has the advantages of quick curative effect, small dosage and low cost on piglet digestive tract diseases.

The parts are parts by mass unless otherwise specified.

In order to achieve the purpose, the technical scheme of the invention is as follows:

the piglet feed additive is prepared by sequentially drying, crushing, sieving and sterilizing eucommia ulmoides leaves, pine needles, glossy privet fruits and semen cuscutae, and then fermenting by using a microbial agent, wherein the microbial agent comprises bacillus licheniformis, clostridium butyricum and bacillus subtilis.

The inventor finds that the feed additive prepared by sequentially drying, crushing, sieving and sterilizing eucommia leaves, pine needles, glossy privet fruits and semen cuscutae and then fermenting by microbial agents (the microbial agents comprise bacillus licheniformis, clostridium butyricum and bacillus subtilis) has quick curative effect on piglet digestive tract diseases, small dosage and low cost in the research process.

The piglets refer to pigs from birth to 30kg (the clinical diagnosis and treatment of piglet diarrhea, Liuxing Yuan, Chinese veterinary abstracts for animal husbandry, No. 3 at volume 32 in 2016, page 201, and 12 months and 31 days in 2016).

The strains used in the application are purchased strains and are provided by China industrial microorganism strain preservation management center. The bacillus licheniformis preservation number is CICC 10831; the clostridium butyricum has a preservation number of CICC 10390; the bacillus subtilis has a preservation number of CICC 20445.

Further, the mixture ratio of the eucommia ulmoides leaves, the pine needles, the glossy privet fruits and the south dodder seeds in parts by mass is as follows: 30-50 parts of eucommia leaves, 20-30 parts of pine needles, 10-20 parts of glossy privet fruits and 5-10 parts of semen cuscutae.

Further, the mixture ratio of the eucommia ulmoides leaves, the pine needles, the glossy privet fruits and the south dodder seeds in parts by mass is as follows: 50 parts of eucommia leaves, 30 parts of pine needles, 15 parts of glossy privet fruits and 5 parts of south dodder seeds.

Further, the drying is specifically drying for 24-36h at 40-60 ℃.

Further, the sieving is specifically 80-100 mesh sieving.

Further, the sterilization is specifically performed at the temperature of 115-123 ℃ for 20-30min, and then the temperature is cooled to 35-40 ℃.

Further, the total number of bacteria in the microbial agent is 0.5 × 10⁸-1.0×10⁸cfu/ml。

Further, the volume ratio of the bacillus licheniformis to the clostridium butyricum to the bacillus subtilis in the microbial agent is 0.8-1.2:0.8-1.2: 0.8-1.2.

Further, the mass ratio of the microbial agent to the traditional Chinese medicine obtained through sterilization treatment is 0.8:1-1.2: 1.

Further, the fermentation is specifically carried out for 48-72h at 30-37 ℃.

Further, the piglet feed additive is prepared by drying 30-50 parts of eucommia ulmoides leaves, 20-30 parts of pine needles, 10-20 parts of glossy privet fruits and 5-10 parts of semen cuscutae at 40-60 ℃ for 24-36 hours, crushing, sieving with 80-100 meshes of sieves, sterilizing at 115-123 ℃ for 20-30 minutes, then cooling to 35-40 ℃, and fermenting by using a microbial agent at 30-37 ℃ for 48-72 hours to obtain the piglet feed additive in parts by mass, wherein the microbial agent comprises bacillus licheniformis, clostridium butyricum and bacillus subtilis, and the total bacterial count in the microbial agent is 0.5 multiplied by 10⁸-1.0×10⁸cfu/ml, and the volume ratio of the bacillus licheniformis to the clostridium butyricum to the bacillus subtilis in the microbial agent is 0.8-1.2:0.8-1.2: 0.8-1.2.

The invention has the beneficial effects that:

the feed additive of the invention has quick curative effect on piglet digestive tract diseases, less dosage and low cost.

The feed additive has good effect of preventing and treating piglet diarrhea.

The feed additive has the functions of disease resistance and growth promotion for growing piglets.

The feed additive can improve the immunity of piglets.

Detailed Description

The examples are provided for better illustration of the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art should make insubstantial modifications and adaptations to the embodiments of the present invention in light of the above teachings and remain within the scope of the invention.

The following bacillus licheniformis is purchased from China industrial microorganism strain preservation management center with the preservation number of CICC 10831;

the following clostridium butyricum is purchased from China industrial microorganism strain preservation management center with the preservation number of CICC 10390;

the following bacillus subtilis is purchased from China industrial microorganism strain preservation management center with the preservation number of CICC 20445.

The following biogenic sources of life and benefit-the special-purpose appetizer biological feed Limited company for pigs, the production batch number is; 20190801

The following immunoglobulin A (IgA) kit is purchased from TSZ Co., Ltd, and the production batch number is CG 2019070188;

the following immunoglobulin g (igg) kits were purchased from TSZ ltd, usa, and the production lot number is CG 2019070193;

immunoglobulin m (igm) kit was purchased from TSZ ltd, usa, and manufactured under the batch number CG 2019070199.