Multi-marker detection magnetic particle luminous micro-fluidic chip and detection device
阅读说明：本技术 一种多标志物检测的磁微粒发光微流控芯片以及检测装置 (Multi-marker detection magnetic particle luminous micro-fluidic chip and detection device ) 是由 王东 江荣香 范玉霞 陈泳鹏 李泉 于 2019-10-11 设计创作，主要内容包括：本发明属于微流控芯片发光免疫检测技术领域,尤其涉及一种多标志物检测的磁微粒发光微流控芯片以及检测装置,包括：顶板,顶板上设有加样部、混合区以及设在混合区中的多个标记配体；底板,底板包括导流区、多个反应区、多个与对应的反应区相互连通的检测区、与检测区相互连通的清洗液存储部和发光液存储部；各个反应区设有磁颗粒配体,各个磁颗粒配体的配体不同；清洗液存储部内部设有清洗液,发光液存储部内存储有发光液；设置在顶板上并用于驱动加样部中的样本流经混合区的气泵。由于反应区设有多个,各个磁颗粒相互不会发生交叉干扰的现象,能够大大提高检测结果的准确性,并且对磁铁的移动速度也没有要求,有利于实现装置的控制。(The invention belongs to the technical field of micro-fluidic chip luminescence immunodetection, and particularly relates to a multi-marker detection magnetic particle luminescence micro-fluidic chip and a detection device, wherein the detection device comprises: the top plate is provided with a sample adding part, a mixing area and a plurality of marking ligands arranged in the mixing area; the bottom plate comprises a flow guide area, a plurality of reaction areas, a plurality of detection areas which are mutually communicated with the corresponding reaction areas, a cleaning liquid storage part and a luminous liquid storage part which are mutually communicated with the detection areas; each reaction zone is provided with a magnetic particle ligand, and the ligands of the magnetic particle ligands are different; the cleaning liquid storage part is internally provided with cleaning liquid, and the luminous liquid storage part stores luminous liquid; and the air pump is arranged on the top plate and used for driving the sample in the sample adding part to flow through the mixing area. Because the reaction zone is provided with a plurality of reaction zones, the phenomenon of cross interference among magnetic particles can not occur, the accuracy of the detection result can be greatly improved, the moving speed of the magnet is not required, and the control of the device is favorably realized.)
1. A magnetic particle luminous micro-fluidic chip for multi-marker detection is characterized by comprising:
the top plate is provided with at least one sample adding part, a mixing region communicated with the sample adding part and a plurality of labeling ligands arranged in the mixing region, and the labeling ligands are different from one another;
the bottom plate comprises a flow guide area communicated with the mixing area, a plurality of reaction areas communicated with the flow guide area, a plurality of detection areas communicated with the corresponding reaction areas, a cleaning liquid storage part and a luminous liquid storage part which are communicated with the detection areas;
each reaction zone is provided with a magnetic particle ligand, and the ligands of the magnetic particle ligands are different;
the cleaning liquid storage part is internally provided with cleaning liquid, and the luminous liquid storage part is internally provided with luminous liquid;
and the air pump is arranged on the top plate and used for driving the sample in the sample adding part to flow through the mixing area.
2. The magnetic particle luminescent microfluidic chip according to claim 1, wherein the mixing region is provided with a labeled ligand storage portion, and the labeled ligand is stored in the labeled ligand storage portion.
3. The magnetic particle luminescent microfluidic chip of claim 2, wherein the number of the sample addition parts and the number of the labeled ligand storage parts are consistent with each other.
4. The magnetic particle luminescent microfluidic chip of claim 1, wherein the sample application part comprises a sample application port and a cover for opening or closing the sample application port, and the sample application part further comprises a rubber ring disposed on the sample application port.
5. The magnetic particle luminescent microfluidic chip according to claim 1, wherein the top plate is provided with a first snap or a first snap, and the bottom plate is provided with a second snap or a second snap, so that the top plate and the bottom plate are fastened to each other by the cooperation of the first snap and the second snap, or the cooperation of the first snap and the second snap.
6. The magnetic particle luminescent microfluidic chip of claim 1, wherein the top plate has magnetically attractive relief holes on corresponding communication tracks with the reaction zone and the detection zone.
7. The magnetic particle luminescent microfluidic chip according to claim 1, wherein the top plate is provided with a cleaning solution relief hole and a luminescent solution relief hole at corresponding positions of the cleaning solution storage part and the luminescent solution storage part.
8. The magnetic particle luminescent microfluidic chip of claim 1, wherein the flow guide region has a groove therein with a height lower than the bottom wall of the reaction region and a flow guide portion disposed on the groove and connected to the reaction region.
9. The magnetic particle luminescent microfluidic chip according to any one of claims 1 to 8, wherein the detection region comprises a plurality of washing regions and a plurality of luminescent regions, which are communicated with each other, and the reaction region, the washing region and the luminescent regions are arranged in one-to-one correspondence.
10. A multi-marker detection magnetic particle luminescence microfluidic detection device, comprising:
the magnetic particle luminescent microfluidic chip of any one of claims 1 to 9;
the magnet unit is used for driving the magnetic particles to move;
a pressing unit for pressing the cleaning liquid storage part, the luminous liquid storage part and the air pump;
a detection unit for detecting a luminescence signal in the detection zone.
Technical Field
The invention belongs to the technical field of micro-fluidic chip luminescence immunodetection, and particularly relates to a multi-marker detection magnetic particle luminescence micro-fluidic chip and a detection device.
Background
Currently, there are two major trends In Vitro Diagnostics (IVD): one is automatic and integrated, namely, the high-precision disease analysis and diagnosis is realized by utilizing full-automatic and high-sensitivity large-scale instruments and equipment of a central laboratory matched with a large-scale hospital; the other type of miniaturization and bedside miniaturization is realized, namely, the rapid analysis and diagnosis on site is realized through handheld small simple equipment. However, small hospitals are not capital intensive, have a small sample size, and are not suitable for purchasing expensive large equipment. Therefore, most of the rapid detection equipment adopted by hospitals at the present stage mainly comprises test strips and corollary equipment thereof, but the test strips can only realize qualitative or semi-quantitative detection, and have the advantages of low detection sensitivity, poor specificity, poor repeatability and obvious interference. Due to the fact that China has a large population, aging is aggravated, the disease incidence is increased sharply, and dependence on large hospitals is overwhelmed. Therefore, the development of a rapid detection method and equipment which are simple and convenient to operate, high in sensitivity, good in repeatability and accurate in quantification becomes urgent.
Chemiluminescence refers to the phenomenon in which chemical energy is converted into light energy by reaction intermediates, reaction products or an additional luminescent reagent in a chemical reaction process. Compared with fluorescence and absorption light, chemiluminescence has no interference of external excitation light source background signals, has small cross interference, and has the advantages of high sensitivity, wide linear range and the like. The chemiluminescence analysis established by the method is widely applied to the fields of clinical diagnosis and the like. The chemiluminescence apparatus is a main large-scale IVD analysis and detection device.
The micro-fluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the biological, chemical and medical analysis process into a micron-scale chip, and automatically completes the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine, etc., it has been developed into a research field with multiple interdisciplinary disciplines of biology, chemistry, medicine, fluid, material, machinery, etc., and is applied to the fields of biomedical research, biochemical detection, judicial appraisal, etc.
For the existing microfluidic chip, when the labeled ligand required by the sample is different, the sample enters a reaction area reacted with the magnetic particle ligand after being mixed with the labeled ligand, and then enters a detection area for quantitatively analyzing and detecting the sample after the reaction. However, all the magnetic particle ligands are placed in the same reaction region, and because the magnetic particle volumes, weights and nuclear-to-mass ratios of different magnetic particle ligands are different, different magnetic particles can be driven to move sequentially by controlling the moving speed of the external magnet, so that the different magnetic particles enter the detection region. Although the moving speed of the magnetic particles is different, especially under the condition that the moving speed of the magnet is higher, the stroke of the magnetic particles is shorter, the phenomenon that the magnetic particles are mutually crossed and interfered is still easily caused, and different labeled ligand solutions are mixed, so that the problem of poor stability exists in the mixing of different magnetic particle ligands, and the accuracy of a detection result is greatly reduced.
Disclosure of Invention
The embodiment of the invention provides a magnetic particle luminous micro-fluidic chip for multi-marker detection and a detection device, and aims to solve the problems that magnetic particles are easy to cross interfere with each other, the stability is poor and the accuracy of a detection result is greatly reduced when the existing magnetic particle luminous micro-fluidic chip is used for detection.
The embodiment of the invention is realized in such a way that a magnetic particle luminous micro-fluidic chip for multi-marker detection is provided, which comprises: the top plate is provided with at least one sample adding part, a mixing region communicated with the sample adding part and a plurality of labeling ligands arranged in the mixing region, and the labeling ligands are different from one another; the bottom plate comprises a flow guide area communicated with the mixing area, a plurality of reaction areas communicated with the flow guide area, a plurality of detection areas communicated with the corresponding reaction areas, a cleaning liquid storage part and a luminous liquid storage part which are communicated with the detection areas; each reaction zone is provided with a magnetic particle ligand, and the ligands of the magnetic particle ligands are different; the cleaning liquid storage part is internally provided with cleaning liquid, and the luminous liquid storage part is internally provided with luminous liquid; and the air pump is arranged on the top plate and used for driving the sample in the sample adding part to flow through the mixing area.
Further, the mixing region is provided with a labeled ligand storage part, and the labeled ligand is stored in the labeled ligand storage part.
Further, the number of the sample addition parts and the number of the labeled ligand storing parts are equal to each other.
Furthermore, the sample adding part comprises a sample adding port and a sealing cover for opening or sealing the sample adding port, and the sample adding part also comprises a rubber ring arranged on the sample adding port.
Furthermore, a first buckle or a first clamping groove is arranged on the top plate, a second clamping groove or a second buckle is arranged on the bottom plate, and the top plate and the bottom plate are buckled with each other through the mutual matching of the first buckle and the second clamping groove or the mutual matching of the first clamping groove and the second buckle.
Furthermore, the top plate is provided with a magnetic attraction abdicating hole on the corresponding communication track of the reaction area and the detection area.
Furthermore, the top plate is provided with a cleaning liquid relief hole and a luminous liquid relief hole at corresponding positions of the cleaning liquid storage part and the luminous liquid storage part.
Furthermore, a groove with the height lower than the bottom wall of the reaction area and a flow guide part which is arranged on the groove and connected with the reaction area are arranged inside the flow guide area.
Furthermore, the detection area comprises a plurality of cleaning areas and a plurality of light emitting areas which are communicated with each other, and the reaction area, the cleaning areas and the light emitting areas are arranged in a one-to-one correspondence manner.
The invention also provides a multi-marker detection magnetic particle luminescence microfluidic detection device, which comprises: the magnetic particle light-emitting microfluidic chip as described above; the magnet unit is used for driving the magnetic particles to move; a pressing unit for pressing the cleaning liquid storage part, the luminous liquid storage part and the air pump; a detection unit for detecting a luminescence signal in the detection zone.
Compared with the prior art, the magnetic particle luminous micro-fluidic chip for multi-marker detection and the detection device provided by the invention have the beneficial effects that a sample enters from the sample adding part of the top plate, the sample and each labeled ligand are mixed in the mixing region, then enter the flow guide region, enter different reaction regions of the bottom plate from the flow guide region, and enter different detection regions from the reaction regions, at the moment, an external magnet drives magnetic particles of the magnetic particle ligands to enter different detection regions, and the luminous detection is realized in the detection regions.
Drawings
FIG. 1 is a schematic diagram of a magnetic particle luminescent microfluidic chip provided with a plurality of labeled ligand storage portions, reaction regions, cleaning regions and luminescent regions according to an embodiment of the present invention;
fig. 2 is a schematic diagram of a magnetic particle luminescent microfluidic chip provided with a plurality of sample adding parts, labeled ligand storage parts, reaction regions, cleaning regions and luminescent regions according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The invention provides a magnetic particle luminous micro-fluidic chip for multi-marker detection and a detection device, wherein a sample enters from a