阅读说明：本技术 一种imac芯片的重生液配方 (Regeneration liquid formula of IMAC chip ) 是由 晏妮 于 2019-10-18 设计创作，主要内容包括：本发明涉及生物芯片技术领域,且公开了一种IMAC芯片的重生液配方,该重生液的配方材料为73.06克乙二胺四乙酸钠,溶于1升去离子水,调节pH值到7.0。该IMAC芯片的重生步骤,将IMAC芯片置入250mM乙二胺四乙酸钠溶液中,经过两个小时的浸泡洗脱以后,可使IMAC芯片重新恢复,可以多次重复使用,从而降低芯片的使用成本。(The invention relates to the technical field of biochips, and discloses a formula of a regeneration liquid of an IMAC chip, wherein the formula material of the regeneration liquid is 73.06 g of sodium ethylene diamine tetracetate, the sodium ethylene diamine tetracetate is dissolved in 1L of deionized water, and the pH value is adjusted to 7.0. The regeneration step of the IMAC chip comprises the steps of putting the IMAC chip into 250mM EDTA solution, and after soaking and elution for two hours, the IMAC chip can be recovered again and can be reused for many times, thereby reducing the use cost of the chip.)

1. The formula of the regeneration liquid of the IMAC chip is characterized in that the final concentration of the sodium ethylene diamine tetracetate is 250mM, and the pH value of the solution is 7.0.

2. The formulation of claim 1, wherein the reconstitution fluid is prepared by:

s1, weighing 73.06 g of sodium ethylene diamine tetracetate with proper weight;

s2, adding 73.06 g of sodium ethylene diamine tetracetate slowly into about 1L of deionized water, and stirring continuously;

s3, after the solution is dissolved, adjusting the pH value of the solution to 7.0;

and S4, filtering the solution to obtain the regeneration liquid.

3. The formulation of claim 1, wherein the reconstitution fluid is formulated for use in an IMAC chip by:

s1, soaking the IMAC chip in a regeneration solution, and culturing the regeneration solution of the IMAC chip in a shaking table for 2 hours;

s2, taking out the IMAC chip, putting the IMAC chip into purified water, and putting the IMAC chip into a shaking table for culturing for 1 hour;

and S3, taking out, and quickly drying through an air dryer.

Technical Field

The invention relates to the technical field of biochips, in particular to a formula of a regeneration liquid of an IMAC chip.

Background

The protein chip technology is a proteomics research method which is simple in operation, convenient and fast, small in sample requirement, high in sensitivity, strong in specificity and high in flux, has wide application prospects in aspects of protein function analysis, tumor marker screening, drug research and the like, is developed by combining mass spectrum according to a chromatography technology, is a solid point surface which is chemically and biologically modified on the surface and can be combined with protein, a metal ion chelating agent is fixed on the surface of an IMAC protein chip, affinity purification is carried out on the protein by utilizing the metal complexing action between metal ions on a carrier and amino acid residues exposed on the surface of the protein, and has good application prospects in separation of escherichia coli expression inclusion body protein and discovery of the tumor marker.

A key factor of the protein chip technology is the selection of the protein chip, different protein chips need to be selected according to different properties of the detected target protein, so that the protein to be detected is specifically combined, non-specific protein is washed away, the detection background is reduced, the use frequency of the protein chip is theoretically disposable, and the use cost of the chip is greatly increased.

Therefore, a regeneration liquid formula of the IMAC chip is provided.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a formula of a regeneration liquid of an IMAC chip.

The invention provides the following technical scheme: a formula of a regeneration liquid of an IMAC chip is characterized in that the regeneration liquid is a sodium ethylene diamine tetracetate solution with the final concentration of 250mM and the pH value is 7.0.

Preferably, the process for preparing the regeneration liquid is as follows:

s1, weighing 73.06 g of sodium ethylene diamine tetracetate with proper weight;

s2, adding 73.06 g of sodium ethylene diamine tetracetate slowly into about 1L of deionized water, and stirring continuously;

s3, after the solution is dissolved, adjusting the pH value of the solution to 7.0;

and S4, filtering the solution to obtain the regeneration liquid.

Preferably, the use method of the regeneration liquid comprises the following steps:

s1, soaking the IMAC chip in a regeneration solution, and culturing the regeneration solution of the IMAC chip in a shaking table for 2 hours;

s2, taking out the IMAC chip, putting the IMAC chip into purified water, and putting the IMAC chip into a shaking table for culturing for 1 hour;

and S3, taking out, and quickly drying through an air dryer.

Compared with the prior art, the invention has the following beneficial effects:

according to the regeneration liquid formula of the IMAC chip, the IMAC chip is placed in the EDTA sodium solution, and after soaking and elution for two hours, the IMAC chip can be recovered again and can be reused for multiple times, so that the use cost of the chip is reduced.

Drawings

FIG. 1 is a schematic diagram of an IMAC chip according to the present invention;

FIG. 2 is a diagram showing the effect of the regenerant of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure will be described below clearly and completely with reference to the accompanying drawings of the embodiments of the present disclosure. It is to be understood that the described embodiments are only a few embodiments of the present disclosure, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the disclosure without any inventive step, are within the scope of protection of the disclosure.

Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this disclosure belongs. The use of the word "comprising" or "comprises", and the like, in this disclosure is intended to mean that the elements or items listed before that word, include the elements or items listed after that word, and their equivalents, without excluding other elements or items.

To maintain the following description of the embodiments of the present disclosure clear and concise, detailed descriptions of known functions and known components are omitted so as to not unnecessarily obscure the concepts of the present disclosure.

A formula of a regeneration liquid of an IMAC chip is characterized in that the regeneration liquid is a sodium ethylene diamine tetracetate solution with the final concentration of 250mM and the pH value is 7.0.

The preparation process of the regeneration liquid is as follows:

s1, weighing 73.06 g of sodium ethylene diamine tetracetate with proper weight;

s2, adding 73.06 g of sodium ethylene diamine tetracetate slowly into about 1L of deionized water, and stirring continuously;

s3, after the solution is dissolved, adjusting the pH value of the solution to 7.0;

and S4, filtering the solution to obtain the regeneration liquid.

The use method of the regeneration liquid comprises the following steps:

s1, soaking the IMAC chip in a regeneration solution, and culturing the regeneration solution of the IMAC chip in a shaking table for 2 hours;

s2, taking out the IMAC chip, putting the IMAC chip into purified water, and putting the IMAC chip into a shaking table for culturing for 1 hour;

and S3, taking out, and quickly drying through an air dryer.

FIG. 1 shows a used chip, in which a large number of specifically bound polypeptide molecules are visible, and FIG. 2 shows the result of the same chip being treated with a regeneration solution, in which the bound polypeptide molecules are completely eluted, so that the IMAC chip is recovered again, and the chip can be reused many times, thereby reducing the cost of using the chip.

The above embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and the scope of the present invention is defined by the claims. Various modifications and equivalents may be made by those skilled in the art within the spirit and scope of the present invention, and such modifications and equivalents should also be considered as falling within the scope of the present invention.