阅读说明：本技术 绿色氨基酸肥料的制备方法 (Preparation method of green amino acid fertilizer ) 是由 李学朋 王伟 田永红 卢松 伏广好 王斌 刘路 于 2019-10-31 设计创作，主要内容包括：本发明属于肥料制备技术领域,公开了绿色氨基酸肥料的制备方法,其特征在于,所述制备方法包括：将氨基酸缓释肥料和无机肥料按照1:5-10的质量比混匀,包装,即得。本发明制备方法中主要使用了发酵废弃物,操作工艺简单,实现了变废为宝。(The invention belongs to the technical field of fertilizer preparation, and discloses a preparation method of a green amino acid fertilizer, which is characterized by comprising the following steps: and uniformly mixing the amino acid slow-release fertilizer and the inorganic fertilizer according to the mass ratio of 1:5-10, and packaging to obtain the fertilizer. The preparation method mainly uses fermentation waste, has simple operation process and realizes changing waste into valuable.)

1. The preparation method of the green amino acid fertilizer is characterized by comprising the following steps: and uniformly mixing the amino acid slow-release fertilizer and the inorganic fertilizer according to the mass ratio of 1:5-10, and packaging to obtain the fertilizer.

2. The method according to claim 1, wherein the inorganic fertilizer is a nitrogen-phosphorus-potassium compound fertilizer.

3. The preparation method of claim 1, wherein the amino acid slow release fertilizer is prepared by the following steps:

separating amino acid fermentation liquor: separating amino acid fermentation liquor, collecting mycoprotein and liquid, extracting amino acid from the liquid, and reserving fermentation wastewater generated in the extraction process for later use;

preparing amino acid metal chelating solution in step (2): drying mycoprotein at 80 ℃, grinding, sieving with a 50-100 mesh sieve, adding an aqueous solution containing calcium salt, manganese salt and zinc salt, keeping the mycoprotein content at 40-100g/L, treating by ultrasonic waves for 8-10min, shearing in a high-speed shearing machine at 10000rpm for 60-80s, stopping shearing, adding neutral protease and papain, keeping the temperature at 50 ℃ and the time at 8-9 h; then heating to 60 ℃, and preserving heat for 1-2 hours to obtain amino acid metal chelating liquid;

preparing carbonized particles in step (3): crushing crop straws to obtain straw powder, sequentially adding the straw powder and bentonite into fermentation wastewater, uniformly mixing, standing for 12 hours, then placing the mixture into a granulator for granulation, placing the granules into a tubular furnace, carbonizing the granules in a nitrogen atmosphere at the temperature of 500 ℃ for 30min, taking out, and naturally cooling to obtain carbonized granules;

and (4) spraying, stirring and packaging: spraying the amino acid metal chelating solution obtained in the step (2) to the carbonized particles obtained in the step (3), uniformly stirring, and drying at 55-65 ℃ to obtain the amino acid metal chelating agent.

4. The method according to claim 3, wherein the concentrations of the calcium salt, the manganese salt and the zinc salt are each 0.03 to 0.05 mol/L.

5. The method of claim 3, wherein the calcium salt is selected from calcium nitrate, calcium chloride, or a mixture thereof.

6. The method as claimed in claim 3, wherein the manganese salt is selected from manganese sulfate, manganese nitrate or a mixture thereof.

7. The preparation method according to claim 3, wherein the zinc salt is zinc sulfate, zinc nitrate or a mixture of the two.

8. The method according to claim 3, wherein the amount of the neutral protease added is 1200U/L, and the amount of the papain added is 1000U/L.

9. The method according to claim 3, wherein the ratio of the amino acid metal chelate solution to the carbonized particles is 1L:4 to 5 kg.

10. The production method according to claim 3, wherein the fermentation wastewater: straw powder: bentonite = 1L: 200-300 g: 150- & ltwbr/& gt 200g.

Technical Field

The invention belongs to the technical field of fertilizer preparation, and particularly relates to a preparation method of a green amino acid fertilizer.

Background

Amino acids in the amino acid fertilizer can be directly used by crops to synthesize protein, nucleic acid and individual endogenous hormones, so that the amino acid fertilizer is good fertilizers with quick effect and good effect, can replace partial nitrogen fertilizers, can enhance photosynthesis, and can change leaf color to dark green, different amino acid types have different functions, wherein aspartic acid can improve seed germination, protein synthesis and provide nitrogen for growth in a stress period, isoleucine and leucine can improve salt stress resistance and improve pollen vitality and germination and aromatic precursor substances, phenylalanine can increase lignification, tip control and anthocyanin of plants, arginine can improve root development, is a precursor for synthesizing plant endogenous hormone polyamine and can improve salt stress resistance of the crops, alanine can increase synthesis of chlorophyll to regulate open photosynthetic pores and resist germs, glycine has a unique effect on crop action, is beneficial to crop growth and increases the content of sugar, tryptophan is a precursor for synthesizing endogenous hormone indole acetic acid and can improve synthesis of aromatic compounds, histidine can regulate open pores and provide a precursor for carbon skeleton hormone to catalyze germination of cells, lysine can catalyze germination of plant growth and improve drought resistance to tyrosine, and can improve drought stress resistance of the plants.

Disclosure of Invention

In order to overcome the defects in the prior art, the invention provides the preparation method of the green amino acid fertilizer, which mainly uses fermentation waste, has simple operation process and low cost, and realizes the purpose of changing waste into valuable.

The invention is realized by the following technical scheme.

The preparation method of the green amino acid fertilizer is characterized by comprising the following steps: and uniformly mixing the amino acid slow-release fertilizer and the inorganic fertilizer according to the mass ratio of 1:5-10, and packaging to obtain the fertilizer.

Preferably, the inorganic fertilizer is a nitrogen-phosphorus-potassium compound fertilizer.

, the amino acid slow release fertilizer is prepared according to the following steps:

separating amino acid fermentation liquor: separating amino acid fermentation liquor, collecting mycoprotein and liquid, extracting amino acid from the liquid, and reserving fermentation wastewater generated in the extraction process for later use;

preparing amino acid metal chelating solution in step (2): drying mycoprotein at 80 ℃, grinding, sieving with a 50-100 mesh sieve, adding an aqueous solution containing calcium salt, manganese salt and zinc salt, keeping the mycoprotein content at 40-100g/L, treating by ultrasonic waves for 8-10min, shearing in a high-speed shearing machine at 10000rpm for 60-80s, stopping shearing, adding neutral protease and papain, keeping the temperature at 50 ℃ and the time at 8-9 h; then heating to 60 ℃, and preserving heat for 1-2 hours to obtain amino acid metal chelating liquid;

preparing carbonized particles in step (3): crushing crop straws to obtain straw powder, sequentially adding the straw powder and bentonite into fermentation wastewater, uniformly mixing, standing for 12 hours, then placing the mixture into a granulator for granulation, placing the granules into a tubular furnace, carbonizing the granules in a nitrogen atmosphere at the temperature of 500 ℃ for 30min, taking out, and naturally cooling to obtain carbonized granules;

and (4) spraying, stirring and packaging: spraying the amino acid metal chelating solution obtained in the step (2) to the carbonized particles obtained in the step (3), uniformly stirring, and drying at 55-65 ℃ to obtain the amino acid metal chelating agent.

Preferably, the concentrations of the calcium salt, the manganese salt and the zinc salt are all 0.03-0.05 mol/L.

Preferably, the calcium salt is selected from calcium nitrate, calcium chloride or a mixture of the two.

Preferably, the manganese salt is manganese sulfate, manganese nitrate or a mixture of the manganese sulfate and the manganese nitrate.

Preferably, the zinc salt is zinc sulfate, zinc nitrate or a mixture of the two.

Preferably, the addition amount of the neutral protease is 1200U/L, and the addition amount of the papain is 1000U/L.

Preferably, the ratio of the amino acid metal chelating solution to the carbonized particles is 1L:4-5 kg.

Preferably, the fermentation wastewater: straw powder: bentonite = 1L: 200-300 g: 150- & ltwbr/& gt 200g.

Preferably, the frequency of the ultrasonic wave is 20-25 kHz.

Compared with the prior art, the invention has the advantages that the following aspects are mainly included but not limited:

the invention adopts ultrasonic treatment and high-speed shearing treatment, can fully crush the cell walls of the thalli, and simultaneously leads partial protein peptide bonds to be broken, thereby being beneficial to the subsequent enzymolysis reaction; according to the invention, metal salt ions are added into the mycoprotein solution, so that the osmotic pressure is improved, the thallus cracking is promoted, the enzyme activity is improved, the metal ions can be used as amino acid chelating metal ions for preparing amino acid chelates, a plurality of technical problems are solved, the enzymolysis and the chelating are carried out simultaneously, and the cost and the operation flow are saved; experiments show that the hydrolysis rate and the amino acid yield can be improved by the compatibility of the neutral protease and the papain.

The method has the advantages that the amino acid wastewater is difficult to treat, the investment cost of enterprises is high, the traditional stacking and burning disposal mode is improved by utilizing the process, the negative influence on the environment is reduced, and the nutrient substances contained in the amino acid wastewater are fully utilized; the carbonized particles prepared by the invention utilize waste water and agricultural wastes, the adsorption effect is enhanced by adding bentonite, amino acid chelates can be adsorbed by coordination, hydrogen bonds, coulomb force and other modes, the defects of amino acid loss and too fast release along with water are avoided, and the carbonized particles can be finally and fully utilized by plants to provide nutrients for the plants.

Drawings

FIG. 1: influence of enzymolysis temperature on amino acid content;

FIG. 2: influence of enzymolysis time on amino acid content.

Detailed Description

In order that those skilled in the art will better understand the technical solutions of the present application, the technical solutions of the present application will be clearly and completely described below in conjunction with the specific embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application , rather than all embodiments.