阅读说明：本技术 一种植物有机营养液 (plant organic nutrient solution ) 是由 刘庆义 于 2019-10-23 设计创作，主要内容包括：本发明涉及农业种植领域,更具体地,本发明涉及一种植物有机营养液。所述植物有机营养液的制备原料包括A组分和B组分；所述A组分按体积百分数计,由3～6％益生菌发酵剂和余量的益生菌培养液组成；所述B组分按重量份计,由0.05～0.5份营养元素和1000份水组成；所述A组分占所述B组分的0.5～1wt％。本发明提供一种植物有机营养液,可以有效抑菌,且提供植物生长必要的元素,从而提高植物的成活率和生长,可用于水果、蔬菜、花卉等多种植物的种植；另外,本发明所述植物有机营养液具有高的铁含量,且通过所述益生菌的作用,可以促进植物对铁的吸收,从而增加植物的品质,可进一步增加对植物的生长和成熟的促进作用。(The invention relates to the field of agricultural planting, in particular to plant organic nutrient solution, which comprises a component A and a component B, wherein the component A comprises 3-6% of probiotic leavening agent and the balance of probiotic culture solution in percentage by volume, the component B comprises 0.05-0.5 part of nutrient elements and 1000 parts of water in parts by weight, and the component A accounts for 0.5-1 wt% of the component B.)

1, plant organic nutrient solution, characterized in that, the preparation raw material of the plant organic nutrient solution comprises A component and B component;

the component A consists of 3-6% of probiotic starter culture and the balance of probiotic culture solution in percentage by volume;

the component B comprises 0.05-0.5 part of nutrient elements and 1000 parts of water in parts by weight;

the component A accounts for 0.5-1 wt% of the component B.

2. The phyto-organic nutrient solution of claim 1, wherein the probiotic starter is selected from or more of lactic acid bacteria starter, yeast bacteria starter, nitrogen-fixing bacteria starter, acetic acid bacteria starter, bifidobacteria starter, photosynthetic bacteria starter and actinomycetes starter.

3. The plant organic nutrient solution as claimed in claim 2, wherein the probiotic starter comprises a lactic acid bacteria starter, a yeast bacteria starter and an acetic acid bacteria starter, and the volume ratio of the lactic acid bacteria starter to the yeast bacteria starter to the acetic acid bacteria starter is 1: (2-3): (2-3).

4. The plant organic nutrient solution as claimed in claim 1, wherein the raw materials for preparing the probiotic culture solution comprise, by weight, 0.5-1% of tea leaves, 2-10% of carbon source and the balance of water, wherein the carbon source is or more selected from glucose, fructose, xylose and starch.

5. The plant organic nutrient solution as claimed in claim 4, wherein the raw materials for preparing the probiotic culture solution further comprise iron source selected from or more of beans, mushrooms and grains.

6. The plant organic nutrient solution as claimed in claim 5, wherein the weight ratio of the iron source to the carbon source is (2-3): (2-4).

7. The plant organic nutrient solution as claimed in claim 5, wherein the iron source is selected from mushrooms selected from or more of flammulina velutipes, shiitake mushrooms, agrocybe aegerita and black fungus.

8. The plant organic nutrient solution of any of claims 1-7, wherein the nutrient elements are selected from or more of potassium salt, magnesium salt, calcium salt and sodium salt.

9, A method for preparing the plant organic nutrient solution according to any of claims 1-8, which comprises the following steps:

preparation of a component A: adding a probiotic starter into a probiotic culture solution, and culturing for 4-6 days at an initial pH of 4.5-5.5 and at a temperature of 30-40 ℃ to obtain the component A;

b, preparation of a component: mixing the nutrient elements with water to obtain the component B;

preparing a plant organic nutrient solution: and mixing the component A and the component B to obtain the plant organic nutrient solution.

The use of plant organic nutrient solutions according to any of claims 1-8 , characterized in that the solutions are used for planting vegetables, fruits and flowers.

Technical Field

The invention relates to the field of agricultural planting, in particular to plant organic nutrient solution.

Background

The production process of the existing plants such as vegetables, fruits, flowers and the like pursues yield maximization, uses a large amount of chemical fertilizers, inhibits the growth of soil microorganisms, destroys the micro-ecological environment, and is easy to generate plant diseases and insect pests, biological stress inhibited by microorganisms and chemical stress caused by overuse of pesticides and chemical fertilizers cause plant organism cell gene mutation, and steps affect the healthy growth of human beings and other animals, and the factors such as pesticide residue for resisting biological diseases, heavy metal pollution caused by industrial development and the like also easily cause chemical pollution to affect food safety.

The nutrient solution is kinds of novel nutrient solution synthesized by adopting an environmental biological and ecological symbiotic technology and a mycorrhizal symbiotic principle through processes of biological fermentation, chemical chelation, physical activation and the like.

Disclosure of Invention

In order to solve the above problems, an th aspect of the present invention provides plant organic nutrient solution, which is prepared from a raw material comprising a component a and a component B;

the component A consists of 3-6% of probiotic starter culture and the balance of probiotic culture solution in percentage by volume;

the component B comprises 0.05-0.5 part of nutrient elements and 1000 parts of water in parts by weight;

the component A accounts for 0.5-1 wt% of the component B.

According to preferable technical schemes, the probiotic leavening agent is selected from or more of lactic acid bacteria leavening agent, yeast leavening agent, nitrogen-fixing bacteria leavening agent, acetic acid bacteria leavening agent, bifidobacterium leavening agent, photosynthetic bacteria leavening agent and actinomycete leavening agent.

According to preferable technical schemes, the probiotic leavening comprises a lactic acid bacteria leavening, a yeast leavening and an acetic acid bacteria leavening, wherein the volume ratio of the lactic acid bacteria leavening, the yeast leavening and the acetic acid bacteria leavening is 1 (2-3) to 2-3.

According to preferable technical schemes, the preparation raw materials of the probiotic culture solution comprise, by weight, 0.5-1% of tea leaves, 2-10% of carbon sources and the balance of water, wherein the carbon sources are or more of glucose, fructose, xylose and starch.

According to preferable technical schemes, the preparation raw materials of the probiotic culture solution also comprise iron sources, and the iron sources are selected from or more of beans, mushrooms and grains.

According to preferable technical schemes, the weight ratio of the iron source to the carbon source is (2-3) to (2-4).

According to preferable technical schemes, the iron source is selected from mushrooms, and the mushrooms are selected from or more of flammulina velutipes, shiitake mushrooms, agrocybe aegerita and black fungus.

According to preferred technical solutions of the invention, the nutrient elements are selected from or more of potassium salt, magnesium salt, calcium salt and sodium salt.

The second aspect of the invention provides methods for preparing the plant organic nutrient solution, which comprises the following steps:

preparation of a component A: adding a probiotic starter into a probiotic culture solution, and culturing for 4-6 days at an initial pH of 4.5-5.5 and at a temperature of 30-40 ℃ to obtain the component A;

b, preparation of a component: mixing the nutrient elements with water to obtain the component B;

preparing a plant organic nutrient solution: and mixing the component A and the component B to obtain the plant organic nutrient solution.

The third aspect of the invention provides applications of the plant organic nutrient solution, which is applied to the planting of vegetables, fruits and flowers.

Compared with the prior art, the plant organic nutrient solution provided by the invention has the beneficial effects that the organic nutrient solution prepared by the invention has high bacteriostatic components such as acetic acid and caffeine and can effectively inhibit bacteria by the combined action of the component A containing probiotics and the component B containing nutrient elements, and elements necessary for plant growth are provided, so that the survival rate and the growth of plants are improved.

Drawings

Fig. 1 is a Py-GCMS map of the plant organic nutrient solution provided in example 3, wherein the retention time RT of fig. 1 is 1.593min on the left, and the retention time RT of fig. 1 is 16.060min on the right.

FIG. 2 is the MS diagram of the plant organic nutrient solution provided in example 3.

Figure 3 is an FTIR plot of the plant organic nutrient solution provided in example 3.

Detailed Description

The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.

The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.

When an equivalent, concentration, or other value or parameter is expressed as a range, preferred range, or as a range defined by the series of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pairing of any upper range limit or preferred value with any lower range limit or preferred value, regardless of whether ranges are separately disclosed.

The singular forms "optional" and "any " include plural referents unless the context clearly dictates otherwise, and it is intended that the items or events described hereinafter may or may not occur, and that the description includes instances where the event occurs and instances where it does not.

Accordingly, numerical values modified by "about", etc. are intended to mean that the invention is not limited to the precise numerical value.

Thus, "" or "" should be read to include or at least , and elements or components in the singular also include the plural unless the number expressly indicates that the singular is meant.

The present invention is illustrated by the following specific embodiments, but is not limited to the specific examples given below.

According to an th aspect of the invention, plant organic nutrient solution is provided, and the preparation raw materials of the plant organic nutrient solution comprise a component A and a component B;

the component A consists of 3-6% of probiotic starter culture and the balance of probiotic culture solution in percentage by volume;

the component B comprises 0.05-0.5 part of nutrient elements and 1000 parts of water in parts by weight;

the component A accounts for 0.5-1 wt% of the component B.

In preferred embodiments, the raw material for preparing the plant organic nutrient solution comprises a component A and a component B;

the component A consists of 5 percent of probiotic starter and the balance of probiotic culture solution according to volume percentage;

the component B comprises 0.5 part of nutrient elements and 1000 parts of water in parts by weight;

the weight ratio of the component A to the component B is 0.8 wt%.

[ A component ]

Probiotic leavening agent

The probiotics are fungi and bacteria beneficial to human body, animals, plants, etc., and can be exemplified by lactic acid bacteria, yeast, acetic acid bacteria, nitrogen-fixing bacteria, bifidobacteria, photosynthetic bacteria, actinomycetes.

In embodiments, the probiotic starter culture of the present invention is selected from the group consisting of or more of a lactic acid bacteria starter culture, a yeast bacteria starter culture, a nitrogen-fixing bacteria starter culture, an acetic acid bacteria starter culture, a bifidobacteria starter culture, a photosynthetic bacteria starter culture, and an actinomycetes starter culture.

Preferably, the probiotic leavening agent comprises a lactic acid bacteria leavening agent, a yeast bacteria leavening agent and an acetic acid bacteria leavening agent, wherein the volume ratio of the lactic acid bacteria leavening agent to the yeast bacteria leavening agent to the acetic acid bacteria leavening agent is 1 (2-3) to (2-3), and is carried out, wherein the volume ratio of the lactic acid bacteria leavening agent to the yeast bacteria leavening agent to the acetic acid bacteria leavening agent is 1: 3: 3.

More preferably, the preparation method of the lactic acid bacteria starter comprises the following steps:

inoculating lactobacillus into the lactobacillus seed solution, and culturing at 30-40 ℃ and 160-230 r/min for 36-60 h to obtain the lactobacillus leavening.

preferably, the preparation method of the lactobacillus leaven of the invention comprises the following steps:

inoculating lactobacillus into the lactobacillus seed solution, and culturing at 35 deg.C and 200r/min for 48 hr to obtain lactobacillus starter.

The lactic acid bacteria seed solution is a lactic acid bacteria culture solution commonly used by those skilled in the art, and includes, but is not limited to, a BCG culture solution, an M17 culture solution, an MRS culture solution and a TJA culture solution.

preferably, the yeast starter of the present invention is prepared by a method comprising the steps of:

the yeast is inoculated into the yeast seed liquid, and cultured for 36-60 h at 30-40 ℃ and 160-230 r/min to obtain the yeast starter.

In preferred embodiments, the yeast starter culture of the present invention is prepared by a method comprising the steps of:

inoculating yeast into yeast seed liquid, and culturing at 35 deg.C and 180r/min for 48 hr to obtain yeast starter.

The yeast seed solution of the present invention is a commonly used yeast culture solution for those skilled in the art, and includes, but is not limited to, YEPD culture solution (yeast extract peptone glucose culture solution), PDA culture solution (potato glucose agar PDA culture solution).

In more preferred embodiments, the method for preparing the acetic acid bacteria starter comprises the following steps:

inoculating acetic acid bacteria into acetic acid bacteria seed solution, and culturing at 35 deg.C and 220r/min for 50h to obtain acetic acid bacteria starter.

The acetic acid bacteria seed solution is an acetic acid bacteria culture solution commonly used by a person skilled in the art, and is prepared by adding water into 30g of glucose, 5.0g of yeast extract, 10g of peptone and 3g of calcium carbonate to a constant volume of 1000mL, subpackaging the obtained mixture into 250mL triangular bottles in 100 mL/bottle, and sterilizing the obtained product at 110-121 ℃ for 10-25 min.

In a preferred embodiment of steps, steps include that the lactobacillus is selected from or more of lactobacillus casei, streptococcus lactis, lactobacillus plantarum, lactobacillus bulgaricus, lactobacillus acidophilus, lactobacillus fermentum, streptococcus thermophilus, leuconostoc mesenteroides and pediococcus acidilactici, steps include lactobacillus plantarum, lactobacillus acidophilus and lactobacillus casei, steps include that the ratio of the lactobacillus plantarum starter culture to the lactobacillus acidophilus starter culture to the lactobacillus casei starter culture is (2-3) to (2-3), and steps include that the ratio of the lactobacillus plantarum starter culture to the lactobacillus acidophilus starter culture to the lactobacillus casei starter culture is 1: 1: 1.

In more preferred embodiments in steps, the yeast of the invention is selected from or more of saccharomyces cerevisiae, saccharomyces inexorable, yeast of the genus leyder, schizosaccharomyces pombe, candida tropicalis, candida krusei, debaryomyces hansenii, brettanomyces, kluyveromyces and zygosaccharomyces bailii, the yeast of the invention comprises saccharomyces cerevisiae and candida krusei in steps, the volume ratio of the saccharomyces cerevisiae starter to the candida krusei starter is (3-4): 1 in steps, and the volume ratio of the saccharomyces cerevisiae starter to the candida krusei starter is 3: 1 in steps.

In further preferred embodiments, the acetic acid bacteria of the present invention are selected from or more of acetobacter xylinum, gluconobacter, acetobacter ketogenes, acetobacter suboxydans, acetobacter gluconicum, acetobacter aceti and acetobacter pasteurianus, and further further, the acetic acid bacteria of the present invention include acetobacter xylinum.

Probiotic culture solution

According to embodiments, the probiotic culture solution provided by the invention comprises raw materials, by weight, 0.5-1% of tea leaves, 2-10% of a carbon source and the balance of water, wherein the carbon source is or more selected from glucose, fructose, xylose and starch.

The tea leaves can be black tea and green tea, wherein the tea leaves contain tea polyphenol, tea pigment, such as chlorophyll, β -carotene, theanine, tea polysaccharide and other substances, and can be used for culturing probiotics.

Preferably, the raw materials for preparing the probiotic culture solution comprise, by weight, 0.7% of tea leaves, 8% of a carbon source and the balance of water.

The applicant finds that when the nutrient solution prepared by adding the component A and the component B of the invention act together, the survival rate and the growth of plants can be promoted, which is probably because when the lactic acid bacteria, the yeast and the acetic acid bacteria are cultured in the probiotic culture solution, the lactic acid bacteria are dominant bacteria, a carbon source is converted into ethanol due to high oxygen content in the early fermentation stage, the ethanol promotes the growth of the acetic acid bacteria, so that the acetic acid bacteria gradually become dominant bacteria, and the lactic acid bacteria can generate a bacterial film, so that the contact between oxygen and the culture solution is prevented in step , the fermentation process mainly adopts anaerobic fermentation, the acetic acid bacteria change ethanol into acetic acid, so that the pH value is reduced, and the adverse effect of harmful bacteria on the growth of the plants is prevented due to the difficulty in the survival of microorganisms under acidic conditions, and in addition, the survival rate of the harmful bacteria is further inhibited in step due to the inclusion of caffeine and other substances in the tea leaves by adding the probiotic culture solution, so that the survival rate of the plants is improved, and the growth of the plants is promoted.

More preferably, the carbon source is starch, the starch is or more of sweet potato starch, corn starch and potato starch, and further the starch is sweet potato starch.

, preferably, the preparation raw materials of the probiotic culture solution also comprise an iron source, wherein the iron source is selected from or more of beans, mushrooms and grains, , the iron source is the mushrooms, the mushrooms are selected from or more of flammulina velutipes, shiitake mushrooms, agrocybe cylindracea and black fungus, and , the mushrooms are the flammulina velutipes.

The needle mushroom is rich in protein, vitamins such as vitamin B and vitamin C, minerals such as iron, amino acids such as arginine and lysine, and polysaccharides such as intracellular polysaccharide.

Further , the weight ratio of the iron source and the carbon source is preferably (2-3): (2-4), and further the weight ratio of the iron source and the carbon source is 3: 4.

Iron is also a trace element essential to plants and the largest trace element required, and is a constituent of cytochrome and non-heme ferritin in photosynthesis, biological nitrogen fixation, and respiration. Slight iron deficiency leads to reduced chlorophyll synthesis and reduced photosynthetic rate; when the iron is seriously deficient, the chlorophyll synthesis is stopped, new leaves turn yellow, and the biomass is greatly reduced. The applicant finds that by adding an iron source into the probiotic culture solution, particularly when the mushrooms are used as the iron source, the mushrooms, particularly the needle mushrooms, contain more iron and can promote the plants to absorb the iron under the action of probiotics, so that the quality and the survival rate of the plants can be improved when the nutrient solution is adopted, and the plants such as fruits, vegetables and flowers are bright in color, fresh green in leaves and full in fruits.

In addition, the applicant finds that when the plant organic nutrient solution obtained by adding the iron source and the carbon source and preparing the component A and the component B to act together is used for culturing plants, the growth and the propagation of the plants can be promoted in steps, which is probably because the iron source, such as flammulina velutipes, contains various saccharides, proteins, vitamins and the like besides the iron element, wherein the saccharides of the flammulina velutipes can act together with the sugar source as the energy of probiotics, when anaerobic fermentation is mainly performed in the later period, the pH value is further reduced in steps along with the increase of acetic acid generated by acetic acid bacteria, and lactic acid bacteria become dominant bacteria and can generate lactic acid, succinic acid and the like, and in addition, yeasts such as saccharomyces cerevisiae and the like can generate succinic acid under the anaerobic condition, so that the growth of the plants is promoted under the combined action of the succinic acid and the lactic acid.

In preferred embodiments, the method of preparing the probiotic culture fluid of the present invention comprises the steps of:

heating water to 100 ℃, cooling to 80-90 ℃, adding tea leaves, preserving heat for 30-40 min, filtering to obtain tea water, adding a carbon source, and mixing to obtain the probiotic culture solution.

In more preferred embodiments, in the method for preparing the probiotic culture solution, the mushroom is pulverized, added with water, ultrasonically treated, filtered to obtain a filtrate, the filtrate, a carbon source and tea water are mixed, and sterilized to obtain the probiotic culture solution.

[ B component ]

In embodiments, the nutrient elements of the present invention are selected from or more of potassium, magnesium, calcium, and sodium salts.

Examples of potassium salts include, but are not limited to, potassium nitrate, potassium iodide.

Examples of magnesium salts include, but are not limited to, magnesium nitrate, magnesium sulfate.

Examples of calcium salts include, but are not limited to, calcium nitrate, calcium superphosphate.

Examples of sodium salts include, but are not limited to, sodium nitrate, sodium dihydrogen phosphate.

Preferably, the nutrient elements comprise potassium salt, magnesium salt, calcium salt and sodium salt, the weight ratio of the potassium salt to the magnesium salt to the calcium salt to the sodium salt is (3-4): (1-2): 1, and the weight ratio of the potassium salt to the magnesium salt to the calcium salt is 3: 1: 1: 1 further .

The applicant finds that when the component B and the component A prepared by adding the nutrient elements disclosed by the invention act together, the prepared plant organic nutrient solution has better effect on the growth of plants and the like, probably because the elements such as potassium, magnesium, calcium and the like are added, and the trace elements such as iron and the like provided by the component A form proper concentration and proportion, so that the growth of the plants can be further promoted .

The second aspect of the present invention provides a method for preparing the plant organic nutrient solution, comprising the following steps:

preparation of a component A: adding a probiotic starter into a probiotic culture solution, and culturing for 4-6 days at an initial pH of 4.5-5.5 and at a temperature of 30-40 ℃ to obtain the component A;

b, preparation of a component: mixing the nutrient elements with water to obtain the component B;

preparing a plant organic nutrient solution: and mixing the component A and the component B to obtain the plant organic nutrient solution.

The third aspect of the invention provides the application of the plant organic nutrient solution, which is applied to the planting of vegetables, fruits and flowers.

The plant organic nutrient solution can be used for planting various plants, such as vegetables, fruits and flowers, can promote the growth and the survival of the plants, and can improve the quality of the plants, so that the fruits, the vegetables and the flowers have bright colors and are full.