阅读说明：本技术 一种利用谷氨酸发酵废弃物制备的复合肥料 (composite fertilizer prepared by glutamic acid fermentation waste ) 是由 程文焕 庄严 卢松 徐娜 杜鹏 周敬 高雷 王斌 于 2019-10-28 设计创作，主要内容包括：本发明属于氨基酸废弃物处理的环保技术领域,公开了一种利用谷氨酸发酵废弃物制备的复合肥料,其按照如下步骤制备而得：步骤1)制备氨基酸螯合颗粒；步骤2)制备尿素-腐植酸络合物；步骤3)制备复合肥料。本发明复合肥料和常规的复合肥搭配,养分配比合理,增产明显,商品性好,并且减少了常规无机肥的使用量,节约了成本,可显著提高经济效益；同时,避免了无机肥大量使用造成的土壤恶化。(The invention belongs to the technical field of environmental protection of amino acid waste treatment, and discloses compound fertilizers prepared by utilizing glutamic acid fermentation waste, which are prepared according to the following steps of 1) preparing amino acid chelate particles, 2) preparing urea-humic acid complex, and 3) preparing compound fertilizers.)

1, kinds of compound fertilizer prepared by using glutamic acid fermentation waste, which is prepared according to the following steps:

step 1) preparing amino acid chelate particles;

step 2) preparing a urea-humic acid complex;

and 3) preparing the compound fertilizer.

2. The compound fertilizer according to claim 1, which is prepared by the following steps:

step 1) preparation of amino acid chelate particles: spraying the amino acid metal chelating solution to the carbonized particles, uniformly stirring, drying at 60 ℃, and naturally cooling to room temperature to obtain amino acid chelating particles;

step 2) preparing a urea-humic acid complex, namely adding urea and water into a stirrer according to the weight ratio of 1:2, heating to 35 ℃, stirring at 200rpm for 15min under the condition of heat preservation, stopping stirring, then adding parts of humic acid accounting for five parts of urea by weight, heating to 85 ℃, stirring at 100rpm for 5min under the condition of heat preservation, and obtaining the urea-humic acid complex;

step 3) preparing a compound fertilizer: spraying the urea-humic acid complex onto the amino acid chelated particles, uniformly stirring, drying at 60 ℃, cooling to room temperature, and packaging to obtain the compound fertilizer.

3. The compound fertilizer according to claim 2, wherein in the step 1), the ratio of the amino acid metal chelating solution to the carbonized particles is 1L:3-10 kg.

4. The compound fertilizer as claimed in claim 2, wherein in the step 3), the mass ratio of the urea-humic acid complex to the amino acid chelated particles is 1L:3-8 kg.

5. The compound fertilizer according to claim 2, characterized in that the amino acid metal chelating solution is prepared by a method comprising:

drying mycoprotein at 80 ℃, grinding, sieving with a 100-mesh sieve, adding an aqueous solution containing calcium salt, manganese salt and zinc salt, keeping the mycoprotein content at 70g/L, treating with 20kHz ultrasonic waves for 10min, shearing in a high-speed shearing machine at 10000rpm for 80s, stopping shearing, adding neutral protease and papain in the addition amounts of 1200U/L and 1000U/L respectively, at 50 ℃ and for 8 h; then heating to 60 ℃, and preserving heat for 1h to obtain the amino acid metal chelating solution.

6. The compound fertilizer as claimed in claim 2, wherein the preparation method of the carbonized particles comprises the following steps: crushing corn straws to obtain straw powder, sequentially adding the straw powder and bentonite into fermentation wastewater, uniformly mixing, standing for 12 hours, then placing the mixture into a granulator for granulation, placing the granules into a tubular furnace, carbonizing the granules under the nitrogen atmosphere at the temperature of 500 ℃ for 30min, taking out, and naturally cooling to obtain carbonized granules.

7. The compound fertilizer according to claim 5 or 6, wherein the mycoprotein and the fermentation wastewater are obtained by: preparing glutamic acid fermentation liquor by utilizing corynebacterium glutamicum fermentation, centrifuging the fermentation liquor, collecting mycoprotein and filtrate, using the filtrate for extracting glutamic acid, and using fermentation wastewater produced in the extraction process for later use.

8. The compound fertilizer according to claim 5, wherein the concentrations of the calcium salt, the manganese salt and the zinc salt are all 0.03 to 0.05 mol/L.

9. The compound fertilizer according to claim 5, wherein the calcium salt is selected from calcium nitrate or calcium chloride; manganese sulfate or manganese nitrate is selected as the manganese salt; the zinc salt is zinc sulfate and zinc nitrate.

10. The compound fertilizer according to claim 6, wherein the fermentation wastewater: straw powder: bentonite = 1L: 300 g: 200 g.

Technical Field

The invention belongs to the technical field of environmental protection of amino acid waste treatment, and particularly relates to compound fertilizers prepared by utilizing glutamic acid fermentation waste.

Background

Glutamic acid is acidic amino acids, contains two carboxyl groups in the molecule, has the chemical name of α -aminoglutaric acid, is discovered in 1856 by Lixon, is colorless crystal, has delicate flavor, is greatly present in cereal protein, and has more content in animal brain.

The fermentation process of producing glutamic acid with corynebacterium glutamicum is one conventional process for producing and consuming gourmet powder, but the production process of gourmet powder has great amount of waste water, and the mother liquid after isoelectrical extraction of glutamic acid from the gourmet powder fermenting liquid has the features of high CODCr, BOD5, high thallus content, high sulfate radical content, high ammonia nitrogen content, low pH value (1.5-3.2), five-high -low.

The applicant's prior patent technology ' methods for producing liquid fertilizer by using glutamic acid wastewater ' discloses that fermentation waste liquid is dried, crushed into powder by a crusher, then placed in a reaction kettle, 8mol/L hydrochloric acid is added, stirring and hydrolyzing are carried out at 60 ℃ for 12 hours under the condition of no raw material, the stirring speed is 100 r/min, after the reaction is finished, residual hydrochloric acid is neutralized by potassium hydroxide to obtain amino acid aqueous solution, then metal ions are added according to the molar ratio of amino acid to metal ions of 3: 1, the temperature is controlled to be 40 ℃, the time is 30min, the pH is 7.0 to carry out chelating reaction, finally the chelate product is concentrated, dried and crushed to obtain amino acid chelate, the amino acid chelate, chitosan, humic acid, urea, potassium dihydrogen phosphate and the concentrated mother liquor obtained in the step 3) are added into a stirring tank according to the mass ratio of 1: 5: 8: 30: 35: 100, stirring is carried out for 10 minutes at 200 r/min, the method adopts strong acid hydrolysis, tryptophan is completely destroyed, amino acid containing hydroxyl such as serine or threonine has small part of 6332, glutamine and side chain is easily hydrolyzed into glutamine, and the amino acid is easily lost along with the hydrolysis, and the total amino acid activity is quickly lost.

Disclosure of Invention

The invention aims to solve the technical problem of providing compound fertilizers prepared by utilizing glutamic acid fermentation wastes.

The invention is realized by the following technical scheme.

kinds of compound fertilizer prepared by using glutamic acid fermentation waste, which is prepared according to the following steps:

step 1) preparing amino acid chelate particles;

step 2) preparing a urea-humic acid complex;

and 3) preparing the compound fertilizer.

, the compound fertilizer is prepared by the following steps:

step 1) preparation of amino acid chelate particles: spraying the amino acid metal chelating solution to the carbonized particles, uniformly stirring, drying at 60 ℃, and naturally cooling to room temperature to obtain amino acid chelating particles;

step 2) preparing a urea-humic acid complex, namely adding urea and water into a stirrer according to the weight ratio of 1:2, heating to 35 ℃, stirring at 200rpm for 15min under the condition of heat preservation, stopping stirring, then adding parts of humic acid accounting for five parts of urea by weight, heating to 85 ℃, stirring at 100rpm for 5min under the condition of heat preservation, and obtaining the urea-humic acid complex;

step 3) preparing a compound fertilizer: spraying the urea-humic acid complex onto the amino acid chelated particles, uniformly stirring, drying at 60 ℃, cooling to room temperature, and packaging to obtain the compound fertilizer.

Preferably, in the step 1), the ratio of the amino acid metal chelating solution to the carbonized particles is 1L:3-10 kg.

Preferably, in the step 3), the mass ratio of the urea-humic acid complex to the amino acid chelated particles is 1L:3-8 kg.

Preferably, the preparation method of the amino acid metal chelating solution comprises the following steps:

drying mycoprotein at 80 ℃, grinding, sieving with a 100-mesh sieve, adding an aqueous solution containing calcium salt, manganese salt and zinc salt, keeping the mycoprotein content at 70g/L, processing by adopting 20kHz ultrasonic waves for 10min, then placing in a high-speed shearing machine to shear for 80s at the speed of 10000rpm, stopping shearing, adding neutral protease (12 ten thousand U/g) and papain (10 ten thousand U/g), wherein the adding amounts are 1200U/L and 1000U/L respectively, the temperature is 50 ℃, and the time is 8 h; then heating to 60 ℃, and preserving heat for 1h to obtain the amino acid metal chelating solution.

Preferably, the preparation method of the carbonized particles comprises the following steps: crushing corn straws to obtain straw powder, sequentially adding the straw powder and bentonite into fermentation wastewater, uniformly mixing, standing for 12 hours, then placing the mixture into a granulator for granulation, placing the granules into a tubular furnace, carbonizing the granules under the nitrogen atmosphere at the temperature of 500 ℃ for 30min, taking out, and naturally cooling to obtain carbonized granules.

Preferably, the mycoprotein and the wastewater are obtained by the following steps: preparing glutamic acid fermentation liquor by utilizing corynebacterium glutamicum fermentation, centrifuging the fermentation liquor, collecting mycoprotein and filtrate, using the filtrate for extracting glutamic acid, and using fermentation wastewater produced in the extraction process for later use.

More preferably, the concentrations of the calcium salt, the manganese salt and the zinc salt are all 0.03-0.05 mol/L.

More preferably, the calcium salt is selected from calcium nitrate or calcium chloride; manganese sulfate or manganese nitrate is selected as the manganese salt; the zinc salt is zinc sulfate and zinc nitrate.

Preferably, the fermentation wastewater: straw powder: bentonite = 1L: 300 g: 200 g.

Compared with the prior art, the invention has the advantages that the following aspects are mainly included but not limited:

the invention adopts ultrasonic treatment and high-speed shearing treatment, can fully crush the cell walls of the thalli, and simultaneously leads partial protein peptide bonds to be broken, thereby being beneficial to the subsequent enzymolysis reaction; according to the invention, firstly, the metal salt is added into the mycoprotein solution, so that the osmotic pressure is improved, the thallus cracking is promoted, the enzyme activity is improved, the metal salt can be used as metal ions for amino acid chelation for preparing amino acid chelates, a plurality of technical problems are solved, the enzymolysis and the chelation are carried out simultaneously, and the cost and the operation flow are saved; experiments show that the hydrolysis rate and the amino acid yield can be improved by the compatibility of the neutral protease and the papain.

The method has the advantages that the amino acid wastewater is difficult to treat, the investment cost of enterprises is high, the traditional stacking and burning disposal mode is improved by utilizing the process, the negative influence on the environment is reduced, and the nutrient substances contained in the amino acid wastewater are fully utilized; the carbonized particles prepared by the invention utilize waste water and agricultural wastes, the adsorption effect is enhanced by adding bentonite, amino acid chelates can be adsorbed by coordination, hydrogen bonds, coulomb force and other modes, the defects of amino acid loss and too fast release along with water are avoided, and the carbonized particles can be finally and fully utilized by plants to provide nutrients for the plants.

Humic acid and urea can generate a complex, gradually decompose and release nitrogen, prolong the fertilizer efficiency, reduce the loss of ammonium nitrogen and have obvious synergistic effect on urea; the invention coats the outer layer of the carbonized particles with the urea-humic acid complex, firstly releases the urea on the outer layer as a base fertilizer which can be applied in the early stage of crops, and then gradually releases the amino acid fertilizer, thereby having good slow release effect and comprehensive and more lasting fertilizer efficiency.

Detailed Description

In order that those skilled in the art will better understand the technical solutions of the present application, the technical solutions of the present application will be clearly and completely described below in conjunction with the specific embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application , rather than all embodiments.