阅读说明：本技术 一种白皮杉醇的制备方法及应用 (Preparation method and application of piceatannol ) 是由 钟华 欧淑影 钟荣 于 2019-09-29 设计创作，主要内容包括：本发明属于生物技术领域,具体涉及一种白皮杉醇的制备方法及应用,所述制备方法包括将百香果清洗后选取果肉,葡萄和蓝莓清洗后与百香果的果肉一起进行粉碎、过滤,得滤汁和滤渣,分别对滤汁和滤渣进行微波-丙酮协同提取,合并提取液,减压浓缩,将浓缩液过硅胶柱,无水丙酮洗脱,冷冻干燥或二氧化碳超临界萃取,即得。本发明提供的提取方法,分别对百香果、葡萄和蓝莓的汁和渣进行提取,大大提高了产品收率,通过微波和丙酮协同提取的方法,进一步提高了产品收率,白皮杉醇的提取量达到2200mg/kg,且该制备方法耗时短,能耗低,适于工业化生产。(The invention belongs to the technical field of biology, and particularly relates to a preparation method and application of piceatannol, wherein the preparation method comprises the steps of cleaning passion fruit, selecting pulp, cleaning grapes and blueberries, crushing and filtering the pulp and the pulp of the passion fruit to obtain filtered juice and filter residue, respectively carrying out microwave-acetone synergistic extraction on the filtered juice and the filter residue, combining extracting solutions, concentrating the extracting solutions under reduced pressure, passing the concentrated solution through a silica gel column, eluting the concentrated solution with anhydrous acetone, and carrying out freeze drying or carbon dioxide supercritical extraction.)

1, A method for preparing piceatannol, which is characterized in that the method comprises the following steps:

(1) selecting passion fruit, grape and blueberry, cleaning with sterile ionized water, taking pulp of the passion fruit, the grape and the blueberry , crushing, and filtering to obtain filtrate and filter residue;

(2) adding 60-80% acetone into the filtrate, microwave extracting for 6-10min, and cooling to room temperature to obtain extractive solution;

(3) adding 80-100% acetone into the filter residue, microwave extracting for 4-6min, cooling to room temperature, and filtering to obtain crude extract;

(4) mixing the extracting solution and the crude extracting solution, and concentrating under reduced pressure to obtain a concentrated solution;

(5) passing the concentrated solution through a silica gel column, and eluting with anhydrous acetone to obtain an eluent;

(6) and (3) freeze-drying the eluent to obtain piceatannol powder, or performing carbon dioxide supercritical extraction on the eluent to obtain piceatannol liquid.

2. The method for preparing piceatannol according to claim 1, wherein in step (1), the weight ratio of passion fruit, grape and blueberry is 6-8:1-2: 1-2.

3. The method of claim 1, wherein in the step (2), the weight ratio of the filtered juice to the 60-80% acetone is 1: 6-8.

4. The method for preparing piceatannol according to claim 1, wherein in step (3), the weight ratio of the residue to the 80-100% acetone is 1: 4-6.

5. The method for preparing piceatannol according to claim 1, wherein the power of the microwave in steps (2) and (3) is 200-250W.

6. The method of claim 1, wherein in step (4), the volume of the concentrated solution is 1/20-1/15 of the combined volume of the extractive solution and the crude extractive solution.

7. The use of the piceatannol powder prepared by the method of claim 1 in anti-leukemia drugs.

8. The use of the piceatannol liquid prepared by the method of claim 1 in health products or cosmetics.

Technical Field

The invention belongs to the technical field of biology, and particularly relates to a preparation method and application of piceatannol.

Background

The research shows that the piceatannol has a plurality of pharmacological activities, such as cancer resistance, cell proliferation resistance, inflammation resistance, immunoregulation, lipid oxidation resistance, bacteria resistance and the like, and the research shows that the piceatannol is a good antioxidant and a good cardiovascular protective agent, and the piceatannol is applied and approved in the fields of health care products, cosmetics, medicines and the like by along with the continuous and deep development.

The passion fruit is also called as eggfruit, is spherical or long-sphere in shape, contains yellow fruit juice and black seeds, contains protein, fat, sugar, multiple vitamins, amino acid, piceatannol and the like, is rich in nutrition, is sour, sweet and delicious, has the fruit fragrance of lemon, mandarin orange, strawberry, smoked plum, pineapple, pomegranate, banana and the like, is vegetarian with the name of beverage king and fruit monosodium glutamate, and is an important raw material and additive for producing fruit juice beverages. Can be processed into fruit juice, jam, fruit jelly and buccal tablet, and can be made into aromatic and delicious beverage, or added into other beverages to improve the quality of the beverage. In addition, piceatannol is found in plants such as grapes, blueberries, sugar cane and the like.

At present, the patent documents for extracting piceatannol from fruits are less, and the content of piceatannol extracted by the existing method for extracting piceatannol from passion fruit is lower.

Disclosure of Invention

The invention aims to provide preparation methods of piceatannol with low cost, simple steps and high content, so as to solve the problems in the background technology.

In order to achieve the technical purpose, the technical scheme of the invention is as follows:

the preparation method of piceatannol comprises the following steps:

(1) selecting passion fruit, grape and blueberry, cleaning with sterile ionized water, taking pulp of the passion fruit, the grape and the blueberry , crushing, and filtering to obtain filtrate and filter residue;

(2) adding 60-80% acetone into the filtrate, microwave extracting for 6-10min, and cooling to room temperature to obtain extractive solution;

(3) adding 80-100% acetone into the filter residue, microwave extracting for 4-6min, cooling to room temperature, and filtering to obtain crude extract;

(4) mixing the extracting solution and the crude extracting solution, and concentrating under reduced pressure to obtain a concentrated solution;

(5) passing the concentrated solution through a silica gel column, and eluting with anhydrous acetone to obtain an eluent;

(6) and (3) freeze-drying the eluent to obtain piceatannol powder, or performing carbon dioxide supercritical extraction on the eluent to obtain piceatannol liquid.

As improvements, in the step (1), the weight ratio of the passion fruit to the grape to the blueberry is 6-8:1-2: 1-2.

As modifications, in step (2), the weight ratio of the filtered juice to the 60-80% acetone is 1: 6-8.

As improvements, in the step (3), the weight ratio of the filter residue to the 80-100% acetone is 1: 4-6.

As modifications, in steps (2) and (3), the power of the microwave is 200-250W.

As modifications, in step (4), the volume of the concentrated solution is 1/20-1/15 of the combined volume of the extracting solution and the crude extracting solution.

Due to the adoption of the technical scheme, the invention has the beneficial effects that:

the preparation method of piceatannol provided by the invention is simple to operate, low in raw material cost, capable of extracting juice and residues of passion fruit, grape and blueberry respectively, greatly improving the product yield, improving the product yield by steps through a microwave and acetone synergistic extraction method, short in time consumption and low in energy consumption, and suitable for industrial production.

Detailed Description

The present invention is described in further detail in with reference to specific embodiments, but it should not be understood that the scope of the above subject matter is limited to the following examples, and that any technique realized based on the teachings of the present invention is within the scope of the present invention.