阅读说明：本技术 一种中药组合物在制备治疗慢性酒精性脂肪性肝炎的药物中的应用 (Application of traditional Chinese medicine composition in preparation of medicine for treating chronic alcoholic steatohepatitis ) 是由 刘成 陶乐 马文婷 吴柳 张玮 杨广越 沈东晓 薛冬英 张洁 于 2019-12-10 设计创作，主要内容包括：本发明涉及一种中药组合物在制备治疗慢性酒精性脂肪性肝炎的药物中的应用,所述中药组合物由大黄、桃仁和地鳖虫制成,所述大黄、桃仁和地鳖虫的重量份配比为：大黄5-15份、桃仁5-15份、地鳖虫3-9份,所述中药组合物是按照以下方法制备而成的：分别将各原料药粉碎,分别用体积浓度10％-30％的乙醇提取1-3次,收集、合并提取液,回收乙醇,即得。本发明将下瘀血汤用于治疗慢性酒精性脂肪性肝炎,取得了良好的疗效,不仅扩大了下瘀血汤的应用范围,也为临床治疗慢性酒精性脂肪性肝炎提供了新的思路与方法。(The invention relates to an application of a traditional Chinese medicine composition in preparing a medicine for treating chronic alcoholic steatohepatitis, wherein the traditional Chinese medicine composition is prepared from rhubarb, peach kernel and ground beetle, and the weight parts of the rhubarb, the peach kernel and the ground beetle are as follows: 5-15 parts of rheum officinale, 5-15 parts of peach kernels and 3-9 parts of ground beetles, wherein the traditional Chinese medicine composition is prepared according to the following method: pulverizing the raw materials respectively, extracting with 10-30 vol% ethanol for 1-3 times, collecting extractive solutions, mixing, and recovering ethanol. The invention uses the blood stasis removing soup for treating chronic alcoholic steatohepatitis, obtains good curative effect, not only expands the application range of the blood stasis removing soup, but also provides a new thought and method for clinically treating chronic alcoholic steatohepatitis.)

1. An application of a traditional Chinese medicine composition in preparing medicines for treating chronic alcoholic steatohepatitis is characterized in that the traditional Chinese medicine composition is prepared from rhubarb, peach kernel and Chinese polyphaga.

2. The application of claim 1, wherein the rhubarb, the peach kernel and the Chinese polyphaga are prepared from the following components in parts by weight: 5-15 parts of rhubarb, 5-15 parts of peach kernel and 3-9 parts of ground beetle.

3. The application of claim 2, wherein the rhubarb, the peach kernel and the Chinese polyphaga are prepared from the following components in parts by weight: 10 parts of rhubarb, 10 parts of peach kernel and 6 parts of ground beetle.

4. The use of claim 1, wherein the Chinese medicinal composition is prepared by the following method: pulverizing the raw materials, extracting with 10-30 vol% ethanol for 1-3 times, collecting extractive solutions, mixing, and recovering ethanol.

5. The use of claim 1, wherein the dosage form of the Chinese medicinal composition is an oral dosage form.

6. The use according to claim 5, wherein the oral dosage form is a capsule, granule, tablet or mixture.

7. The use of claim 1, wherein the Chinese medicinal composition further comprises a pharmaceutically conventional carrier.

8. Use according to claim 7, wherein the pharmaceutically conventional carrier is selected from one or more of the group consisting of emulsifiers, excipients, fillers, binders, humectants, disintegrants, absorption enhancers, flavourings, colourings and cosolvents.

Technical Field

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to application of a traditional Chinese medicine composition in a medicine for treating chronic alcoholic steatohepatitis.

Background

Alcoholic Liver Disease (ALD) refers to liver damage caused by long-term excess of alcohol, initially manifested as significant adipocyte steatosis, which may later progress to steatohepatitis, hepatic fibrosis and cirrhosis. According to the difference of liver damage degree of patients, the liver damage treatment method is divided into the following types: mild alcoholic liver disease: slight abnormalities in one or more of liver biochemical markers, imaging and histological findings; alcoholic fatty liver: fatty liver can be seen in the imaging examination, and the biochemical indexes of the liver can be slightly abnormal; alcoholic hepatitis: mainly shows that the level of serum transaminase and glutamyl transpeptidase is increased, which is usually accompanied by the increase of serum bilirubin and fever and peripheral blood neutrophilic granulocytosis; alcoholic liver fibrosis: symptoms and signs of a patient and common laboratory and ultrasonic examination and the like are not changed characteristically, the serum hepatic fibrosis index and liver instantaneous imaging examination elasticity value can be increased, and further diagnosis is confirmed through liver biopsy; alcoholic cirrhosis: the liver cirrhosis has symptoms and signs of liver cirrhosis, the elasticity value of liver instantaneous imaging examination is obviously increased, and the imaging changes of ultrasound, CT and the like accord with the liver cirrhosis. Alcoholic liver disease belongs to the category of 'wine injury', 'wine addiction', 'wine drum' and 'alcoholic jaundice' in traditional Chinese medicine. The cause of the disease is the pathogenic factors of alcohol-toxin dampness-heat. The disease is first in the liver, gallbladder, spleen and stomach, and then in the kidney. The pathogenesis of the disease is that damp-heat and alcohol-toxin damage a human body, which causes liver depression and qi stagnation, water-dampness failure, phlegm turbidity generation, liver and spleen blood stasis and viscera deficiency.

The decoction for removing blood stasis comes from Furen recorded in Zhang Zhongjing jin Jian Yao L ü e. The original prescription is rhubarb, radix et rhizoma Rhei, radix Et rhizoma Rhei, semen Persicae, 20 Eupolyphaga seu steleophaga, feet are decocted, the three flavors are added, the powder is added, honey is refined, and the mixture is made into four pills, one liter of wine is used for decocting one pill, and the eight pills are taken. It can be used for treating abdominal pain due to stagnation of blood. Modern medicine popularizes and uses the decoction for removing blood stasis, for example, hepatitis with blood stasis symptom, liver cirrhosis, cerebral concussion sequelae, apoplexy sequelae, etc. However, the application of the blood stasis removing decoction to the treatment of chronic alcoholic steatohepatitis is not reported. Although there are reports in the literature that rhubarb, peach kernel and/or Chinese polyphaga in the decoction for removing blood stasis are used for treating alcoholic liver disease, only 1-2 of the Chinese medicines are contained and are often combined with other traditional Chinese medicines, such as Jiaxiuqin (Jiawei Wendan decoction for treating alcoholic fatty liver 38 cases [ J xiuqin]Chinese and Western medicine combined liver disease journal, 1996,6(2):34) and modified Wendan decoction (20 g each of fructus Aurantii Immaturus, purified pinellia Tuber, Coptidis rhizoma, 15g each of Poria, etc., 12g each of pericarpium Citri Tangerinae, caulis Bambusae in Taenia, semen Persicae, bupleuri radix, radix Paeoniae Rubra, radix Paeoniae alba, 30g each of Saviae Miltiorrhizae radix and fructus crataegi, 24g of carapax Trionycis) for treating alcoholic fatty liver; zhoubao et al (Zhoubao, Liujian, Fangjiang Yong, etc.. Jiekang granule for treating chronic alcoholic liver disease 30 cases [ J]Journal of liver disease combining traditional Chinese and western medicine 2002,12(3): 181-. In addition, an article published earlier in this subject group (Cheng Liu, Xia Yuan, Le Tao. Xia-yu-xue-xuedeCotion (XYXD)) reduce carbon tetrachloride (CCl)₄) -induced blood stasis activating by targeting NF-. kappa.B and dTGF- β 1signaling pathways BMC Complementary and Alternative Medicine 2015,15:201) discloses that ecchymoma-discharging decoction is effective in treating CCl₄Induced liver fibrosis. An article published in the early stage of this subject group (Wuliu, Zhang Jie, mahencing, et al. decoction for removing blood stasis in mice for inhibition of non-alcoholic steatohepatitis induced by choline methionine deficiency [ J]Chinese journal of liver diseases (electronic edition), 2018,10(03):54-61) reported that the decoction for removing blood stasis has a significant improvement effect on MCD-induced nonalcoholic steatohepatitis (NASH) in mice. In this article, the MCD-induced NASH model is an 8-week mouse, which is accompanied by liver fibrosis, and it is difficult to determine whether the effect of the decoction for removing blood stasis on the improvement of the mouse model is based on the treatment of liver fibrosisThe application is as follows. Furthermore, the causes and mechanisms of chronic alcoholic steatohepatitis and non-alcoholic steatohepatitis are greatly different, so that it is difficult to predict the effect of the stagnant blood-draining decoction on chronic alcoholic steatohepatitis.

Disclosure of Invention

The invention aims to provide the application of a traditional Chinese medicine composition in preparing medicines for treating chronic alcoholic steatohepatitis, aiming at the defects in the prior art.

In order to achieve the purpose, the invention adopts the technical scheme that:

an application of a Chinese medicinal composition in preparing a medicament for treating chronic alcoholic steatohepatitis is disclosed, wherein the Chinese medicinal composition is prepared from rhubarb, peach kernel and Chinese polyphaga.

As a preferred example of the invention, the ratio of the rhubarb, the peach kernel and the ground beetle in parts by weight is as follows: 5-15 parts of rhubarb, 5-15 parts of peach kernel and 3-9 parts of ground beetle.

More preferably, the weight parts of the rhubarb, the peach kernel and the Chinese polyphaga are as follows: 10 parts of rhubarb, 10 parts of peach kernel and 6 parts of ground beetle.

As another preferred example of the present invention, the Chinese medicinal composition is prepared according to the following method: pulverizing the raw materials, extracting with 10-30 vol% ethanol for 1-3 times, collecting extractive solutions, mixing, and recovering ethanol.

As another preferred example of the present invention, the dosage form of the traditional Chinese medicine composition is an oral dosage form.

More preferably, the oral dosage form is a capsule, granule, tablet or mixture.

As another preferred example of the present invention, the Chinese medicinal composition further comprises a pharmaceutically conventional carrier.

More preferably, the pharmaceutically conventional carrier is selected from one or more of emulsifiers, excipients, fillers, binders, humectants, disintegrating agents, absorption promoters, flavoring agents, coloring agents and solubilizing agents.

The invention has the advantages that:

the decoction for removing blood stasis consists of rhubarb, peach kernel and ground beetle, has the efficacies of removing blood stasis, invigorating blood circulation, purging and stimulating the menstrual flow, and is used for treating postpartum abdominal pain caused by blood stasis and blood stasis stagnation of women. In the recipe, rhubarb, bitter in flavor and cold in flavor, is mainly used for removing blood stasis, breaking abdominal mass and accumulating, and has new effect of aging; peach kernel, semen Persicae is bitter and neutral in taste, is mainly used for treating blood stasis and blood stasis, and has the effects of promoting blood circulation, removing blood stasis and moistening dryness; the ground beetle is good at breaking stasis and eliminating abdominal mass, attacking blood stasis and opening blood stasis, and has the combined effects of promoting blood circulation and removing blood stasis, and softening hardness and dissipating stagnation. The invention uses the blood stasis removing soup for treating the chronic alcoholic steatohepatitis in the alcoholic liver disease, obtains good curative effect, not only expands the application range of the blood stasis removing soup, but also provides a new thought and method for clinically treating the chronic alcoholic steatohepatitis.

Drawings

FIG. 1: example 1 main technical roadmap for animal experiments.

FIG. 2: 10 days chronic alcoholic steatohepatitis model mice liver ratio, serum ALT, AST and TG histograms.

FIG. 3: 4 weeks chronic alcoholic steatohepatitis model mice liver ratio, serum ALT, AST and TG histograms.

FIG. 4: histopathological changes in liver (HE, × 200 fold).

FIG. 5: liver tissue was oil red stained (x 400).

FIG. 6 is a bar graph showing the relative expression amounts of TNF- α and IL-1 β mRNA in mouse liver tissue.

FIG. 7: immunohistochemistry measured MPO expression in liver tissue (. times.400).

Detailed Description

The following detailed description of the present invention will be made with reference to the accompanying drawings.