阅读说明：本技术 一种提高自体脂肪移植成活率的方法 (Method for improving autologous fat transplantation survival rate ) 是由 张红芳 刘中国 任正天 于 2019-10-15 设计创作，主要内容包括：本发明涉及一种提高自体脂肪移植成活率的设备,其包括：包括混合腔体(1)以及重力辅助混合器(2)；混合腔体(1)包括碗形部分、位于碗形部分外侧的水冷腔、与碗形部分连通的加料通道(15)以及位于碗形部分内的搅拌模块(14)；重力辅助混合器(2)包括曲线回转形成的表面。能够保护脂肪细胞活性,并能够充分地将辅助成分与脂肪细胞混合,从而提高自体脂肪移植成活率。(The invention relates to a device for improving the survival rate of autologous fat transplantation, which comprises: comprises a mixing cavity (1) and a gravity-assisted mixer (2); the mixing cavity (1) comprises a bowl-shaped part, a water-cooling cavity positioned on the outer side of the bowl-shaped part, a feeding channel (15) communicated with the bowl-shaped part and a stirring module (14) positioned in the bowl-shaped part; the gravity assisted mixer (2) comprises a curved revolution forming surface. Can protect the activity of fat cells, and can fully mix auxiliary components with the fat cells, thereby improving the survival rate of autologous fat transplantation.)

1. The utility model provides an improve autologous fat transplantation survival rate's equipment which characterized in that: it includes: comprises a mixing cavity (1) and a gravity-assisted mixer (2); the mixing cavity (1) comprises a bowl-shaped part, a water-cooling cavity positioned on the outer side of the bowl-shaped part, a feeding channel (15) communicated with the bowl-shaped part and a stirring module (14) positioned in the bowl-shaped part; the gravity assisted mixer (2) comprises a curved revolution forming surface.

2. The apparatus for improving the survival rate of autologous fat transplantation according to claim 1, wherein: the mixing cavity (1) comprises a shell (11), a water cooling cavity (12), a mixing inner cavity (13), a stirring module (14), a feeding channel (15) and an output channel (16).

3. The apparatus for improving the survival rate of autologous fat transplantation according to claim 2, wherein: the shell (11) can be of a sandwich structure, the outermost layer of the sandwich structure is made of metal or high polymer materials, the middle layer of the sandwich structure is made of heat insulation materials, and the inner layer of the sandwich structure is made of stainless steel materials.

4. The apparatus for improving the survival rate of autologous fat transplantation according to claim 2, wherein: the water inlet pipeline of the water cooling cavity (12) enters the water cooling cavity (12) through an opening positioned below the side wall of the shell (11), and the water outlet pipeline of the water cooling cavity (12) leaves the water cooling cavity (12) through an opening positioned above the side wall of the shell (11).

5. The apparatus for improving the survival rate of autologous fat transplantation according to claim 4, wherein: the central axis of the opening on the side wall of the shell (11) corresponding to the water outlet pipeline of the water cooling cavity (12) does not exceed the height of the intersection of the feeding channel (15) and the outer wall of the mixing inner cavity (13).

6. The apparatus for improving the survival rate of autologous fat transplantation according to claim 1, wherein: the feed channel (15) includes an adipocyte feed channel (15) and an additive feed channel (15).

7. The apparatus for improving the survival rate of autologous fat transplantation according to claim 6, wherein: are symmetrically arranged in the two feed channels (15).

8. The apparatus for improving the survival rate of autologous fat transplantation according to claim 1, wherein: the stirring module (14) comprises a sealing shaft tube (141) which transversely penetrates through the shell (11), the water cooling cavity (12) and the mixing inner cavity (13), and a bearing and a rotating shaft (142) which are arranged on the sealing shaft tube (141).

9. The apparatus for improving the survival rate of autologous fat transplantation according to claim 8, wherein: the shape of the stirring blade (143) is semicircular; the material is an elastic rubber material, and a hydrophobic and oleophobic protective layer is arranged on the outer surface of the elastic rubber material.

10. A method for improving survival rate of autologous fat transplantation based on the apparatus for improving survival rate of autologous fat transplantation according to any one of claims 1 to 9, the method comprising: the adipocyte mass is mixed with the additive ingredients by the above-described apparatus.

Technical Field

The invention relates to the technical field of biology, in particular to equipment and a method for improving the survival rate of autologous fat transplantation.

Background

Autologous fat transplantation techniques have been widely used, for example: enlarging breast, enlarging nose or repairing scar. Compared with the traditional artificial tissue substitute, the autologous fat has the advantages of good compatibility, simple and convenient operation, good filling appearance, small surgical wound, rich source and the like. However, the problem of low survival rate of fat transplantation has always plagued the relevant field of fat transplantation. Although the complications of autologous fat transplantation have been significantly reduced as the technology matures, the survival rate of cell transplantation has been improved in the prior art by improving the liposuction method and the cryopreservation conditions. In recent years, it has been shown that the survival rate of fat transplantation is improved by improving the activity of transplanted adipocytes by adding an auxiliary component, such as a vascular stroma component, platelet-rich plasma, or adipose-derived stem cells, and mixing the auxiliary component with adipocytes.

However, in the process of adding the auxiliary components, the procedure of processing the adipocytes is increased, so that the risk of damage to the adipocytes and deterioration of performance is increased, and in addition, the auxiliary components are not uniformly mixed with the adipocytes, and thus the effect of the auxiliary components is not sufficiently exerted due to improper mixing methods, which causes a problem that the improvement of the survival rate of transplantation is not significant.

Therefore, it is required to provide an apparatus and a method for protecting the activity of adipocytes and sufficiently mixing the auxiliary components with adipocytes, thereby improving the survival rate of autologous fat transplantation.

Disclosure of Invention

The technical problem to be solved by the invention is that in the prior art: in the process of adding the auxiliary components to the adipocytes, the adipocytes are easily damaged, the performance is deteriorated, and the problem of uneven mixing of the auxiliary components and the adipocytes occurs.

The technical scheme adopted by the invention for solving the technical problems is as follows:

an apparatus for increasing the survival rate of autologous fat transplantation, comprising: comprises a mixing cavity 1 and a gravity-assisted mixer 2; the mixing cavity 1 comprises a bowl-shaped part, a water-cooling cavity positioned at the outer side of the bowl-shaped part, a feeding channel 15 communicated with the bowl-shaped part and a stirring module 14 positioned in the bowl-shaped part; the gravity assisted mixer 2 comprises a curved revolution forming surface.

Further, the mixing chamber 1 comprises a shell 11, a water cooling chamber 12, a mixing inner chamber 13, a stirring module 14, a feeding channel 15 and an output channel 16.

Further, the housing 11 may be a sandwich structure, the outermost layer of the sandwich structure is made of metal or polymer material, the middle layer is made of heat insulating material, and the inner layer is made of stainless steel material.

Furthermore, the water inlet pipeline of the water cooling cavity 12 enters the water cooling cavity 12 through an opening positioned below the side wall of the shell 11, and the water outlet pipeline of the water cooling cavity 12 leaves the water cooling cavity 12 through an opening positioned above the side wall of the shell 11.

Furthermore, the central axis of the opening on the side wall of the shell 11 corresponding to the water outlet pipeline of the water cooling cavity 12 does not exceed the height of the intersection of the feeding channel 15 and the outer wall of the mixing inner cavity 13.

Further, the charging passage 15 includes an adipocyte charging passage 15 and an additive component charging passage 15.

Further, the two charging channels 15 are symmetrically arranged.

Further, the stirring module 14 includes a sealed shaft tube 141 transversely penetrating the housing 11, the water cooling chamber 12 and the mixing chamber 13, and a bearing and a rotating shaft 142 mounted on the sealed shaft tube 141

Further, the stirring blade 143 has a semicircular shape; the material is an elastic rubber material, and a hydrophobic and oleophobic protective layer is arranged on the outer surface of the elastic rubber material.

A method for improving the survival rate of autologous fat transplantation based on the device for improving the survival rate of autologous fat transplantation, which comprises the following steps: the adipocyte mass is mixed with the additive ingredients by the above-described apparatus.

The equipment and the method for improving the survival rate of autologous fat transplantation, provided by the invention, have the following beneficial effects: can protect the activity of fat cells, and can sufficiently mix auxiliary components with fat cells.

Drawings

Fig. 1 is a schematic structural diagram of a mixing device provided by the present invention.

Fig. 2 is a schematic structural diagram of a gravity-assisted mixer 2 of the mixing apparatus provided by the present invention.

Detailed Description

The present invention will now be described in more detail with reference to the accompanying drawings, in which preferred embodiments of the invention are shown, it being understood that one skilled in the art may modify the invention herein described while still achieving the beneficial results of the present invention. Accordingly, the following description should be construed as broadly as possible to those skilled in the art and not as limiting the invention.

In the interest of clarity, not all features of an actual implementation are described. In the following description, well-known functions or constructions are not described in detail since they would obscure the invention in unnecessary detail. It will of course be appreciated that in the development of any such actual embodiment, numerous implementation-specific details must be set forth in order to achieve the developer's specific goals.

In order to make the objects and features of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. It is to be noted that the drawings are in a very simplified form and are intended to use non-precision ratios for the purpose of facilitating and clearly facilitating the description of the embodiments of the invention.

The invention provides a mixing device, which is improved to protect fat cells and improve mixing efficiency in the process of mixing the fat cells and auxiliary components, thereby improving the survival rate of fat transplantation.

Fig. 1 is a schematic structural diagram of a mixing device provided by the present invention. The mixing device comprises a mixing chamber 1 and a gravity assisted mixer 2.

The mixing cavity 1 comprises a shell 11, a water cooling cavity 12, a mixing inner cavity 13, a stirring module 14, a feeding channel 15 and an output channel 16.

The housing 11 is used to protect the entire mixing chamber 1, and may be made of a metal material such as stainless steel, or a polymer material with a certain rigidity such as resin or plastic. Preferably, the housing 11 may be a sandwich structure, the outermost layer of the sandwich structure is made of metal or polymer material, the middle layer is made of heat insulating material, and the inner layer is made of stainless steel material. Therefore, the chemical stability of the inner wall of the shell 11 is ensured, the damage of low temperature and cooling water to the inner wall of the shell 11 is avoided, and the temperature of the cooling water is not easy to drift by adding heat insulating materials.

Between the inner wall of the housing 11 and the outer wall of the mixing chamber is a water cooling chamber 12 which maintains the mixing chamber 13 at a suitable process temperature by storing circulating flowing cooling water, thereby avoiding performance degradation of fat cells or other active substances due to excessive temperatures. Preferably, the water cooling chamber 12 is at a temperature greater than 0 degrees and less than 4 degrees. The water inlet line (not shown) of the water cooling chamber 12 enters the water cooling chamber 12 through an opening located below the side wall of the housing 11, and the water outlet line (not shown) of the water cooling chamber 12 exits the water cooling chamber 12 through an opening located above the side wall of the housing 11, so that the circulation of cooling water is realized.

Preferably, in order to provide a reasonable level of cooling water, the central axis of the opening of the side wall of the housing 11 corresponding to the water outlet pipeline of the water cooling cavity 12 does not exceed the height of the intersection of the feed channel 15 and the outer wall of the mixing cavity 13. If the opening position is too high, unnecessary cooling water is wasted, increasing the power of the apparatus, and if the opening position is too low, the whole fat cells cannot be completely cooled.

The mixing chamber 13 has an outer wall formed of an aluminum material, and preferably, an inner wall of the outer wall facing the inside of the mixing chamber 13 may be formed with an aluminum oxide passivation layer, thereby ensuring chemical stability of the chamber wall.

The inner surface shape of the outer wall includes an upper cylindrical portion and a lower bowl portion. The cylindrical part is mainly used for snap-fit mounting with the device housing 11, while the bowl-shaped part is used for receiving and mixing fat cells and additional ingredients. The feed channel 15 meets the outer wall of the mixing chamber 13 at two ends of the upper opening of the bowl. So that the adipocytes and additive components in a semi-viscous state flowing out of the feed channel 15 can flow to the lower part of the mixing chamber 13 along the inner wall of the bowl-shaped portion, thereby avoiding unnecessary adipocyte damage caused by the impact of the adipocytes falling freely to the bottom of the mixing chamber under the action of gravity.

Preferably, the height of the bowl-shaped part is not more than 20 cm, included angles between tangents of points on an arc line of the longitudinal section shape of the side wall of the bowl-shaped part and a horizontal plane are changed along with the height, wherein the included angles are changed to 5-20 degrees every 1 cm, and the selection of the parameters is combined with the physical states of the fat cell mass and the added components, so that the probability that the fat cell mass slides down along the side wall of the bowl-shaped part can be improved, and the protection of fat cells in the processing process is improved.

The charging channel 15 includes an adipocyte charging channel 15 and an additive component charging channel 15. In one embodiment, the two charging channels 15 are symmetrically arranged; the extending direction of the tail end of the charging channel 15 forms an included angle with the horizontal plane; preferably, the included angle is greater than 60 degrees and less than 80 degrees. Too large or too small an angle also tends to cause the fat cells to flow out with too great a horizontal or vertical velocity to slide down the side walls of the mixing chamber. In another embodiment, the angle of the two feed channels 15 can also be set differently depending on the viscosity of the two components, if there is a large difference between the viscosity of the additive component and the viscosity of the fat cell mass.

In the lower space of the mixing chamber 13 (the height of the bowl-shaped portion is 5-10 cm), a stirring module 14 is provided, and the stirring module 14 includes a sealed shaft tube 141 transversely passing through the housing 11, the water cooling chamber 12, and the mixing chamber 13, and a bearing (not shown) mounted on the sealed shaft tube 141 and a rotating shaft 142 connected to a rotating motor for rotation. On a portion of the rotating shaft 142 located in the mixing chamber 13, a plurality of stirring blades 143 are further installed. The plurality of stirring blades 143 may be fixedly attached to the rotating shaft 142 by a hollow sleeve, or may be individually riveted to the rotating shaft 142.

The stirring blade 143 is preferably semicircular in shape. The material is elastic rubber material, and preferably, a hydrophobic and oleophobic protective layer is arranged on the outer surface of the elastic rubber material of the stirring blade 143. The number of vanes may be 6, 8, 10, etc. The rotation speed of the stirring blade 143 is 10 to 25 revolutions per minute. If the rotation speed is too fast, the fat cells are easily damaged, and if the rotation speed is too slow, the stirring effect is affected, which may reduce the uniformity of the mixture of the additive components and the fat cells.

The central axes of the two feeding channels 15 and the central axis of the transmission shaft of the stirring module 14 intersect at the middle point of the central axis of the transmission shaft of the stirring module 14, so that most of falling fat particles can be fully contacted with the stirring blades 143, and full mixing is realized.

At the bottom of the mixing chamber 13, there is a discharge spout 131, and the discharge spout 131 is a funnel-shaped structure with a gradually decreasing diameter. Through the discharge nozzle 131, the preliminary-mixed fat cells and additive component mixture are output. An electromagnetic control valve is arranged between the upper end of the discharging nozzle 131 and the mixing inner cavity 13, when the valve is closed in the mixing process, the mixing inner cavity is a closed space, and when the mixture is output, the valve is controlled to be opened through an external controller to form an output path. The discharging mouth 131 is sleeved with the output channel 16, and the length of the output channel 16 is not more than 4 cm. Below the outlet channel 16 a gravity assisted mixer 2 is arranged.

As shown in fig. 2, the gravity-assisted mixer 2 has a cylindrical structure as a whole. It includes: a cylindrical housing 3 and a rotator fixed to an inner surface of the cylindrical housing 3. The body of revolution comprises a flat surface conforming in shape to the inner surface of the cylindrical shell 3, and a curved revolution surface facing inwards. The curved surface of revolution is formed by a 360 degree rotation of the curve 4 about the central axis O. The curve 4 includes, in order from top to bottom, a first curve 41 in which the slope of the tangent line gradually increases, a second curve 42 in which the slope of the tangent line gradually decreases, a first straight line portion 43, a second straight line portion 44, and a third straight line portion 45. In the curved rotating body, the opening formed by rotating the topmost end of the first curve 41 is larger than the opening formed by rotating the bottommost end of the second curve 42, and the size of the opening formed by rotating the topmost end of the first curve 41 is 1.1-1.5 times of the diameter of the output channel 16. The inclination (slope) of the first straight line portion 43 is smaller than the inclination (slope) of the second straight line portion 44; the inclination (slope) of the second straight line portion 44 is smaller than the inclination (slope) of the third straight line portion 45.

The elastic through hole 5 is provided at a position where the first curve 41 and the second curve 42 are joined, that is, at a position where the opening size is smallest in the bodies of revolution of the first curve 41 and the second curve 42.

The elastic through hole 5 comprises a clamping groove 51 positioned at the corresponding position of the curve revolving body and a circular through hole part 52 arranged inside the clamping groove 51, and the side wall of the inner ring of the opening of the through hole part 52 is chamfered or rounded, so that the fat particles are prevented from being scratched. The diameter of the inner ring of the opening hole is 2-4 mm. The material of the through hole part is elastic rubber material, and an oleophobic and hydrophobic material layer is arranged on the surface.

Through the design of the curve revolving body and the elastic through hole, in the falling process of the fat from the mixing inner cavity 13, along with the falling of the fat, the space between the fat and the elastic through hole in the output channel 16 is gradually reduced, and the passing rate of the elastic through hole is smaller, so that the air pressure between the fat and the elastic through hole in the output channel 16 is slightly increased, and the opening of the first curve revolving body is gradually reduced, and the air pressure is further increased. When the cellulite further falls, the gas with slightly higher air pressure can pass through the elastic through hole, enters the curve revolving body part with gradually increased opening, the gas containing space is increased suddenly, the gas pressure is reduced slightly, namely, the air pressure at the lower part of the elastic through hole is lower, the air pressure at the upper part is high, the air pressure can be realized to drive the fat cells with certain viscosity and the mixture of the added ingredients to pass through the fine elastic through hole, the further fall is realized, the further granulation of the fat is realized, the contact area in the mixing of the fat and the added ingredients is improved, and the mixing effect is improved.

The method for improving the survival rate of autologous fat transplantation based on the equipment comprises the following steps:

in the first step, the fat particle group and the additive component, which may be a vascular stroma component, platelet rich plasma, or adipose-derived stem cells, are added through two feeding channels 15, respectively. The above feeding is carried out in such a manner that the feeding rate of the fat particles does not exceed 10 ml/min.

And secondly, the fat-containing particle clusters and the added components fall into the mixing cavity 13 and are stirred by the stirring module 14, the rotating speed of the stirring blade 143 is 10-25 circles/minute, and the stirring time is not more than 3 minutes.

Step three, after stirring, opening a valve positioned above the output channel, and outputting the mixture;

fourthly, the mixture falls into the gravity assisted mixer 2 through the output channel 16, and in the falling process of the fat from the mixing inner cavity 13, along with the falling of the fat, the space between the fat and the elastic through holes in the output channel 16 is gradually reduced, and the passing rate of the elastic through holes is smaller, so that the gas pressure between the fat and the elastic through holes in the output channel 16 is slightly increased, and the opening of the first curve revolving body is gradually reduced, and the gas pressure is further increased. When the cellulite further whereabouts, the slightly higher gas of atmospheric pressure can pass the elasticity through-hole, gets into the curve solid of revolution part that the opening increases gradually, and gas accommodation space increases suddenly, causes gaseous atmospheric pressure to reduce slightly, and the atmospheric pressure of elasticity through-hole lower part is lower promptly, and upper portion atmospheric pressure is high, can realize that atmospheric pressure drives the adipocyte that has certain viscidity and adds the composition mixture and pass through tiny elasticity through-hole, further whereabouts.

Comparative test:

the animal experiments were performed by treating the apparatus provided by the invention (panel 1) and mixing the fat particles and additive components (platelet rich plasma, prepared by centrifugation at 1100 × g for 10min with addition of calcium chloride activation using gradient centrifugation) by conventional hand (panel 2), mixing autologous adipose tissue with platelet rich plasma at a ratio of 9: 1. A total of 30 healthy rats were selected, and the number of males and females was not limited. The mass of the body is (350 +/-10) g; mice were aged (3.0. + -. 0.5) months. Groups were randomized into 2, and subcutaneous fat grafts were performed all in the upper abdomen, with 0.5 g of fat mix per rat.

Immediately after the fat graft was taken out 2 weeks after the fat transplantation, the wet weight was measured with an electronic balance; then performing paraffin section HE staining, respectively taking transplanted fat from the experimental group and the control group, observing the section of capillary blood vessels to be dark red, performing blood vessel density measurement, observing the HE stained section under a high power lens (x 400), and moving the section for 1 time every 1mm by adopting an equidistant and random sampling method for each section; the average was calculated by taking 4 fields per slice.

Performing statistical analysis by using software, comparing the quality of the transplanted fat of the experimental group 1 and the experimental group 2 with the density of blood vessels, wherein the quality of the transplanted fat of the experimental group 1 is (0.3123 +/-0.0239) g 2 weeks after the transplantation; the mass of experimental group 2 was (0.1340 ± 0.0297) g; 2 weeks after transplantation, the density of the fat microvasculature of the experimental group 1 was (4.08 + -0.49) pieces/mm 2; experimental group 2 the fat microvascular density was (2.79. + -. 0.35) pieces/mm 2. The survival rate of fat transplantation in experimental group 1 was high compared with that in experimental group 2.

The equipment and the method for improving the survival rate of autologous fat transplantation, provided by the invention, have the following beneficial effects: can protect the activity of fat cells, and can sufficiently mix auxiliary components with fat cells.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.