阅读说明：本技术 一种便携式荧光光谱仪 (Portable fluorescence spectrometer ) 是由 吴刚 刘本康 林峰 于乃森 赵慧琳 韩星 于 2019-11-20 设计创作，主要内容包括：本发明公开了一种便携式荧光光谱仪,属于光谱分析装置技术领域。包括,积分球支架,作为被测量样品的样品池以及对激发光源和荧光光谱进行均匀化处理的积分球,光路传输所使用的第一光纤、第二光纤和第三光纤,光谱仪,光强调节器以及为荧光光谱的测量提供激发光源的激光光源。本发明的有益效果是：可以充分克服传统吸收光谱仪在液体测量方面的灵敏度、准确性等特性方面的不足,从而大大提升仪器的性能。(The invention discloses a portable fluorescence spectrometer, and belongs to the technical field of spectrum analysis devices. The device comprises an integrating sphere support, an integrating sphere, a first optical fiber, a second optical fiber and a third optical fiber, a spectrometer, a light intensity regulator and a laser light source, wherein the integrating sphere is used as a sample pool of a measured sample and used for carrying out homogenization treatment on an excitation light source and a fluorescence spectrum, the spectrometer is used for light path transmission, and the laser light source is used for providing the excitation light source for the measurement of the fluorescence spectrum. The invention has the beneficial effects that: the defects of the traditional absorption spectrometer in the aspects of sensitivity, accuracy and other characteristics in the aspect of liquid measurement can be fully overcome, and therefore the performance of the instrument is greatly improved.)

1. A portable fluorescence spectrometer is characterized by comprising an integrating sphere support (1), a sample cell used as a measured sample, an integrating sphere (2) for carrying out homogenization treatment on an excitation light source and a fluorescence spectrum, a first optical fiber (3a), a second optical fiber (3b) and a third optical fiber (3c) used for optical path transmission, a spectrometer (4), a light intensity regulator (5) and a laser light source (6) for providing the excitation light source for the measurement of the fluorescence spectrum;

the integrating sphere support (1) supports and fixes the integrating sphere (2), a height adjusting mechanism for adjusting the height of the integrating sphere (2) is arranged on the integrating sphere support (1), the integrating sphere (2) comprises a hollow sphere composed of an integrating sphere base (2b), a sample support (2a) and an incident light hole (2d) are respectively arranged at the top and the side of the hollow sphere, an emergent light hole (2f) is arranged at the bottom of the hollow sphere, an integrating sphere inner coating (2c) is arranged on the inner wall of the hollow sphere, a light barrier (2e) is arranged in the hollow sphere, the light barrier (2e) is positioned above the emergent light hole (2f), and the incident light hole (2d) is positioned above the light barrier (2 e);

the laser light source (6) is connected with the light intensity regulator (5) through the third optical fiber (3c), the light intensity regulator (5) is connected with the incident light hole (2d) through the second optical fiber (3b), and the emergent light hole (2f) is connected with the spectrometer (4) through the first optical fiber (3 a).

2. The portable fluorescence spectrometer according to claim 1, wherein the integrating sphere support (1) has a height of 10cm to 200 cm.

3. The portable fluorescence spectrometer according to claim 1, wherein the integrating-sphere inner coating (2c) is white and made of barium sulfate or polytetrafluoroethylene, and the thickness of the integrating-sphere inner coating (2c) is 0.1mm to 5 mm.

4. The portable fluorescence spectrometer according to claim 1, wherein the sample holder (2a) is detachably fixed with a cylindrical sample cell or a cuvette sample cell by a bolt, and the surface of the bolt is white.

5. The portable fluorescence spectrometer according to claim 1, wherein the surface of the light barrier (2e) is provided with a white coating, and the white coating is made of barium sulfate or polytetrafluoroethylene.

6. The portable fluorescence spectrometer of claim 1, wherein the input aperture (2d) and the output aperture (2f) have optical fiber quick connectors coupled to their covers, the optical fiber quick connectors are SMA or FC connectors, the inner surface of the optical aperture cover is coated with a white coating, and the coating is made of barium sulfate or polytetrafluoroethylene.

7. The portable fluorescence spectrometer according to claim 1, wherein the first (3a), second (3b) and third (3c) optical fibers are silica fibers, transmit a spectral range of 190nm to the infrared band, and have a core diameter of 0.01mm to 1.0 mm.

8. The portable fluorescence spectrometer according to claim 1, wherein the hollow sphere is made of aluminum alloy or stainless steel, the diameter of the hollow sphere is 20mm to 2500mm, and the outer surface of the integrating sphere substrate (2b) is coated with a black coating.

9. The portable fluorescence spectrometer according to claim 1, characterized in that the spectral detection range of the spectrometer (4) is 190nm to 1100 nm.

Technical Field

The invention relates to a spectrum analysis device, in particular to a portable fluorescence spectrometer capable of accurately measuring the fluorescence spectrum of a solution.

Background

With the development of economy and the continuous improvement of the quality of life of people, the analysis technology, especially the fluorescence spectrum technology, has very important application in the scientific research fields, such as material science, physics and chemistry, biology and medicine and other high-end fields, and plays an increasingly important role in various civil fields such as food sanitation, environmental safety and the like which are closely related to people.

When a substance is excited to its own excited state by a laser beam of a short wavelength, an energy radiation process for transition from the excited state of the substance to the base occurs, and the accompanying radiation light during this transition from a high energy level to a low energy level is called fluorescence. The fluorescence spectrum technology can analyze the concentration information of the substance according to the characteristic spectrum of the measured substance by contrasting the fluorescence intensity of the measured substance, and can make final diagnosis on the analyzed substance, has the outstanding advantages of high efficiency, high speed, low cost and the like, and is widely applied to the related fields of scientific research and daily life. However, with the development of technology, the level of technology associated with absorption spectroscopy technology is also increasing, and the requirements for the corresponding technical standards are becoming more stringent.

The traditional liquid phase measurement fluorescence spectrum technology is usually excited by laser, a light path design scheme for collecting fluorescence in the vertical direction is adopted, and the sensitivity of an analyzed substance is limited to a certain extent by the fluorescence spectrum obtained based on the scheme. Due to the anisotropy of the physicochemical properties of the substance to be measured, especially the liquid substance, the optical path excitation vertical sampling mode loses the rationality of completely and accurately measuring the substance, so that the result of the measured substance is not a real result.

Disclosure of Invention

The present invention has been made in view of the above circumstances, and it is an object of the present invention to provide a portable fluorescence spectrometer which can have more excellent sensitivity, accuracy, and convenience than a conventional fluorescence spectrometer in measuring a liquid substance.

The technical scheme of the invention is as follows: a portable fluorescence spectrometer comprises an integrating sphere support, an integrating sphere, a first optical fiber, a second optical fiber and a third optical fiber, a spectrometer and a light intensity regulator, wherein the integrating sphere is used as a sample cell of a measured sample and used for homogenizing an excitation light source and a fluorescence spectrum;

the integrating sphere support supports and fixes the integrating sphere, a height adjusting mechanism for adjusting the height of the integrating sphere is arranged on the integrating sphere support, the integrating sphere comprises a hollow sphere consisting of an integrating sphere substrate, a sample support and an incident light hole are respectively arranged at the top and the side of the hollow sphere, an emergent light hole is arranged at the bottom of the hollow sphere, an integrating sphere inner coating is arranged on the inner wall of the hollow sphere, a light barrier is arranged in the hollow sphere and is positioned above the emergent light hole, and the incident light hole is positioned above the side of the light barrier;

the laser light source is connected with the light intensity regulator through a third optical fiber, the light intensity regulator is connected with the incident light hole through a second optical fiber, and the emergent light hole is connected with the spectrometer through a first optical fiber.

The height of the integrating sphere support is 10cm-200 cm.

The inner coating of the integrating sphere is white, the material of the inner coating is barium sulfate or polytetrafluoroethylene, and the thickness of the inner coating of the integrating sphere is 0.1mm to 5 mm.

Cylindrical sample cell or cell sample cell are fixed on the sample support through bolt detachable, the bolt surface is white.

The surface of the light barrier is provided with a white coating, and the white coating is made of barium sulfate or polytetrafluoroethylene.

The optical hole covers of the incident optical hole and the emergent optical hole are coupled with optical fiber quick connectors, the optical fiber quick connectors are SMA or FC connectors, the inner surface of each optical hole cover is coated with a white coating, and the coating is made of barium sulfate or polytetrafluoroethylene.

The first optical fiber, the second optical fiber and the third optical fiber are all quartz optical fibers, the transmission spectrum range is 190nm to an infrared band, and the fiber core diameter is 0.01mm to 1.0 mm.

The hollow sphere is made of aluminum alloy or stainless steel, the diameter of the hollow sphere is 20mm to 2500mm, and the outer surface of the integrating sphere substrate is coated with a black coating.

The spectral detection range of the spectrometer is 190nm to 1100 nm.

The invention has the beneficial effects that: the defects of the traditional absorption spectrometer in the aspects of sensitivity, accuracy and other characteristics in the aspect of liquid measurement can be fully overcome, and therefore the performance of the instrument is greatly improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is a graph showing fluorescence spectra of cadmium selenide quantum dots measured according to the present invention.

The reference numbers in the figures are as follows: 1-integrating sphere support, 2-integrating sphere, 2 a-sample support, 2 b-integrating sphere substrate, 2 c-integrating sphere internal coating, 2 d-incident light hole, 2 e-light barrier, 2 f-emergent light hole, 3 a-first optical fiber, 3 b-second optical fiber, 3 c-third optical fiber, 4-spectrometer, 5-light intensity regulator and 6-laser light source.

Detailed Description

The invention is further illustrated with reference to the accompanying figures 1-2:

a portable fluorescence spectrometer comprises an integrating sphere support 1, a sample cell used as a measured sample, an integrating sphere 2 for carrying out homogenization treatment on an excitation light source and a fluorescence spectrum, a first optical fiber 3a, a second optical fiber 3b and a third optical fiber 3c used for optical path transmission, a spectrometer 4, a light intensity regulator 5 and a laser light source 6 for providing an excitation light source for the measurement of the fluorescence spectrum; the integrating sphere support 1 supports and fixes the integrating sphere 2, a height adjusting mechanism for adjusting the height of the integrating sphere 2 is arranged on the integrating sphere support 1, the height of the integrating sphere support 1 is 10cm-200cm, the integrating sphere 2 comprises a hollow sphere composed of an integrating sphere substrate 2b, a sample support 2a and an incident light hole 2d are respectively arranged at the top and the side of the hollow sphere, an emergent light hole 2f is arranged at the bottom of the hollow sphere, an integrating sphere inner coating 2c is arranged on the inner wall of the hollow sphere, a light barrier 2e is arranged in the hollow sphere, the light barrier 2e is positioned above the emergent light hole 2f, and the incident light hole 2d is positioned above the light barrier 2 e; the laser source 6 is connected with the light intensity regulator 5 through the third optical fiber 3c, the light intensity regulator 5 is connected with the incident light hole 2d through the second optical fiber 3b, and the emergent light hole 2f is connected with the spectrometer 4 through the first optical fiber 3 a. The integrating sphere inner coating 2c is white, is made of barium sulfate or polytetrafluoroethylene, and has a thickness of 0.1mm to 5 mm. Cylindrical sample cell or cell sample cell are fixed on the sample support 2a through bolt detachable, the bolt surface is white. The surface of the light barrier 2e is provided with a white coating, and the white coating is made of barium sulfate or polytetrafluoroethylene. The unthreaded hole covers of the incident unthreaded hole 2d and the emergent unthreaded hole 2f are coupled with optical fiber quick connectors, the optical fiber quick connectors are SMA or FC connectors, the inner surfaces of the unthreaded hole covers are coated with white coatings, and the coatings are made of barium sulfate or polytetrafluoroethylene. The first optical fiber 3a, the second optical fiber 3b and the third optical fiber 3c are all quartz optical fibers, the transmission spectrum range is 190nm to an infrared band, and the fiber core diameter is 0.01mm to 1.0 mm. The hollow sphere is made of aluminum alloy or stainless steel, the diameter of the hollow sphere is 20mm to 2500mm, and the outer surface of the integrating sphere substrate 2b is coated with a black coating. The spectral detection range of the spectrometer 4 is 190nm to 1100 nm.

The light intensity adjuster 5 can adjust the light intensity by changing the effective area of the incident light, or can also change the incident light intensity by introducing an attenuation medium; the presence of the intensity adjuster 5 ensures that the spectrum detected by the spectrometer is in the non-saturated region, thereby ensuring that a true and relatively good signal-to-noise spectrum result is obtained. The light barrier 2e completely shields the collection angle of the optical fiber in the emergent pupil, thereby ensuring that the emergent pupil can only receive the light component which is scattered from the inner coating of the integrating sphere and enters the emergent pupil; the integrating sphere 2 is divided into two parts by a light barrier 2e, wherein the sample holder 2a and the entrance pupil 2d are located on the same side of the integrating sphere interior, and the exit pupil 2f is located on the other side of the light barrier of the integrating sphere 2. The range of excitation wavelengths that the laser source 6 can provide is selected according to the absorption spectrum of a specific sample, and in order to ensure the portability of the fluorescence spectrometer, a small semiconductor laser can be used as the typical laser source 6.

As an example of the present invention, the integrating sphere 2 was designed to have a diameter of 15 cm, and was fabricated as a hollow sphere from an aluminum alloy, in which the outer side was treated with metal oxide black and the inner side was coated with a barium sulfate coating, the coating thickness being 0.8 mm. The integrating sphere is provided with 3 through holes, one of the through holes is connected with the sample support, and the other two through holes are respectively connected with the incident optical fiber and the emergent optical fiber. Wherein, an SMA905 optical fiber quick connector is arranged between the pipeline and the unthreaded hole cover, and the sample cell fixed by the sample support used in the embodiment is a cylindrical sample cell with the diameter of 5mm and the height of 30 mm. The adopted laser light source is a 400nm semiconductor laser, the power is 50mW, and the light intensity is adjusted by adjusting the size of a light through hole of the attenuation adjuster. In order to improve the portability of the instrument, the adopted spectrometer is a fiber spectrometer, the resolution is 0.2nm, and the sampling rate per second is not lower than 50 times. Based on the above arrangement, the fluorescence spectrum of the cadmium selenide quantum dot is tested for the tested object by using the cadmium selenide quantum dot, and the fluorescence spectrum of the cadmium selenide quantum dot shown in fig. 2 is obtained specifically.

The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.