阅读说明：本技术 一种鱼种期鉴别杂交黄颡鱼分离群体的方法 (Method for identifying separation group of hybrid pelteobagrus fulvidraco in fish breeding stage ) 是由 周华兴 江河 段国庆 汪焕 胡玉婷 凌俊 陈小雷 潘庭双 于 2019-11-04 设计创作，主要内容包括：本发明提供一种鱼种期鉴别杂交黄颡鱼分离群体的方法,该方法通过大量形态学数据的比对分析,选择了判别力最高的尾柄长、眼径2个形态学特征进行计算,判别鱼是属于偏母群体还是偏父群体,该方法可直接在生产一线通过简单的形态学测量,区分开杂交黄颡鱼分离群体,无需购买复杂的分子试剂和精密的仪器,只需要一把游标卡尺即可,操作简单,成本投入小；在杂交黄颡鱼鱼种时期即可区分分离群体,避免了养殖过程中的成本投入,且准确率可达90％以上；对鱼种伤害小,仅需测量少数的形态学数据,测量过程迅速,测量时间短,测量后不会影响鱼种的成活率。(The invention provides a method for identifying a separation group of hybrid pelteobagrus fulvidraco in a fish breeding period, which selects 2 morphological characteristics of tail handle length and eye diameter with highest discrimination to calculate through comparison and analysis of a large amount of morphological data, and discriminates whether the fish belongs to a partial parent group or a partial parent group, can directly distinguish the separation group of the hybrid pelteobagrus fulvidraco through simple morphological measurement at the production line, does not need to purchase complex molecular reagents and precise instruments, only needs a vernier caliper, is simple to operate and has low cost investment; the separated groups can be distinguished in the fish breeding period of the hybrid pelteobagrus fulvidraco, the cost investment in the breeding process is avoided, and the accuracy can reach more than 90%; the damage to the fingerlings is small, only a few morphological data need to be measured, the measuring process is rapid, the measuring time is short, and the survival rate of the fingerlings cannot be influenced after the measurement.)

1. A method for identifying a separation group of hybrid pelteobagrus fulvidraco in a fish breeding period is characterized by comprising the following steps:

1) selecting a hybrid pelteobagrus fulvidraco as a measurement object individual, and measuring the tail handle length L1 and the eye diameter length L2 of the hybrid pelteobagrus fulvidraco;

2) the measured index parameters of the length L1 of the tail handle and the eye diameter L2 of the yellow catfish are respectively substituted into a formula

Partial population of parents: y1 is 0.933 × L1+65.755 × L2-14.754,

partial father group: y2 is 7.744 XL 1+33.086 XL 2-10.416,

3) comparing Y1 and Y2, such as Y1> Y2, wherein the measured individuals are partial maternal populations; if Y1< Y2, the individual is a partial paternal population.

2. The method for identifying the separation group of the hybrid pelteobagrus fulvidraco in the fish breeding stage according to claim 1, which is characterized in that: the length of the individual to be measured of the hybrid pelteobagrus fulvidraco in the step 1) is 5-8 cm.

3. The method for identifying the separation group of the hybrid pelteobagrus fulvidraco in the fish breeding stage according to claim 1, which is characterized in that: measuring the length L1 of the tail handle and the length L2 of the eye diameter of the hybrid pelteobagrus fulvidraco by using a vernier caliper in the step 2).

Technical Field

The invention relates to a method for identifying aquaculture offspring seeds, in particular to a method for identifying a separation group of hybrid pelteobagrus fulvidraco in a fish breeding period.

Background

The yellow catfish breeding in China starts in the nineties of the last century, and the breeding varieties of the yellow catfish are changed from common yellow catfish to full-male yellow catfish and even to hybrid yellow catfish which is mainly bred at present in more than twenty years of industrial development.

The hybrid pelteobagrus fulvidraco is a hybrid produced by artificial induced spawning, insemination and the like by using pelteobagrus fulvidraco (Tachysurus fulvidraco) as a female parent and pelteobagrus vachellii (Tachysurus vachellii) as a male parent. The parent pelteobagrus fulvidraco is small in size and slow in growth speed, but is favored by the consumer market due to the bright body color and tender meat quality. The pelteobagrus vachelli as the male parent has relatively large body type and high growth speed, but the market acceptance degree is low. The hybrid yellow catfish variety is developed by combining excellent properties of parents so as to obtain hybrid filial generation with high growth speed, bright body color and tender meat. However, as the parent and the maternal plant belong to different species, the genetic relationship is far, the genetic background difference is large, the hybrid progeny inevitably has character differentiation, and separate populations are generated, namely, the body color of most progeny inherits the maternal plant, and the body color of a small part of progeny inherits the paternal plant. The population with the differentiated character is easy to distinguish in the middle and later period of cultivation. The commercial fish is irregular in size, a small part of the commercial fish is like male parent pelteobagrus vachelli, the market acceptance is low, and the commercial fish is not easy to sell and causes loss. Therefore, if the separated groups can be identified in the fish breeding period, the specification uniformity of the commercial fish can be improved, the breeding cost can be reduced, and the breeding benefit can be improved.

At present, common methods for group identification comprise morphological clustering, microsatellite typing, SNP analysis and the like, and the methods usually need a large amount of data analysis and are high in cost investment and difficult to develop in production practice.

Disclosure of Invention

In view of the above-mentioned disadvantages of the prior art, the present invention aims to provide a method capable of accurately identifying the segregating population of the hybrid pelteobagrus fulvidraco in the fingerling period.

In order to achieve the above objects and other related objects, the present invention provides a method for identifying a separation group of hybrid pelteobagrus fulvidraco in a fish breeding stage, which is characterized by comprising the following steps:

1) selecting a hybrid pelteobagrus fulvidraco as a measurement object individual, and measuring the tail handle length L1 and the eye diameter length L2 of the hybrid pelteobagrus fulvidraco;

2) the measured index parameters of the length L1 of the tail handle and the eye diameter L2 of the yellow catfish are respectively substituted into a formula

Partial population of parents: y1 is 0.933 × L1+65.755 × L2-14.754,

partial father group: y2 is 7.744 XL 1+33.086 XL 2-10.416,

3) comparing Y1 and Y2, such as Y1> Y2, wherein the measured individuals are partial maternal populations; if Y1< Y2, the individual is a partial paternal population.

Preferably, the length of the individual measuring object of the hybrid pelteobagrus fulvidraco in the step 1) is 5-8 cm.

Preferably, the length of the tail handle of the hybrid pelteobagrus fulvidraco L1 and the length of the eye diameter L2 are measured by a vernier caliper in the step 2).

As described above, the present invention has the following advantageous effects: 1) the separation groups of the hybrid pelteobagrus fulvidraco can be directly distinguished in the production line through simple morphological measurement, a complex molecular reagent and a precise instrument do not need to be purchased, only a vernier caliper is needed, the operation is simple, and the cost investment is low; 2) the separated groups can be distinguished in the fish breeding period of the hybrid pelteobagrus fulvidraco, the cost investment in the breeding process is avoided, and the accuracy can reach more than 90%; 3) the damage to the fingerlings is small, only a few morphological data need to be measured, the measuring process is rapid, the measuring time is short, and the survival rate of the fingerlings cannot be influenced after the measurement.

Drawings

Fig. 1 is a schematic diagram of morphological characteristics of pelteobagrus fulvidraco.

Wherein L1 is caudal peduncle length, and L2 is eye diameter.

Detailed Description

The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.