阅读说明：本技术 一种化学发光免疫分析系统用磁分离机构 (Magnetic separation mechanism for chemiluminescence immunoassay system ) 是由 何宗平 于 2019-09-26 设计创作，主要内容包括：本发明公开一种化学发光免疫分析系统用磁分离机构,包括清洗盘和位于清洗盘上方并且绕自身轴线转动的清洗转盘,反应杯底端的清洗盘上端面固定安装有多组用于偏移反应杯内磁珠的吸附磁铁组,吸附磁铁组沿着清洗转盘转动方向依次包括：至少一个位于反应杯位一侧的磁铁,以及位于反应杯位另一侧的磁铁；将磁铁按一定规律安装在清洗盘上,使得在清洗分离时磁铁位于反应杯底部一侧,反应杯底面为磁珠提供了很好的支撑,保证了在对反应物清洗的过程中,磁珠既能快速分离,磁珠颗粒也不容易被清洗过程中的吸针吸走,磁珠在清洗过程中在杯子底部局部移动,从而能将沉淀于磁珠下面的杂质释放出来,从而保证了测量结果的可靠性和重复性。(The invention discloses a magnetic separation mechanism for a chemiluminescence immunoassay system, which comprises a cleaning disc and a cleaning turntable, wherein the cleaning turntable is positioned above the cleaning disc and rotates around the axis of the cleaning turntable, a plurality of groups of adsorption magnet groups for offsetting magnetic beads in a reaction cup are fixedly arranged on the upper end surface of the cleaning disc at the bottom end of the reaction cup, and the adsorption magnet groups sequentially comprise the following components in the rotation direction of the cleaning turntable: at least one magnet positioned on one side of the reaction cup position and a magnet positioned on the other side of the reaction cup position; install magnet on wasing the dish according to certain law for magnet is located reaction cup bottom one side when wasing the separation, and the reaction cup bottom surface provides fine support for the magnetic bead, has guaranteed in to the abluent in-process of reactant, and the magnetic bead can quick separation, and the magnetic bead granule is also difficult to be siphoned away by the suction needle among the cleaning process, and the magnetic bead removes at cup bottom part local in the cleaning process, thereby can release the impurity that deposits below the magnetic bead, thereby has guaranteed measuring result's reliability and repeatability.)

1. A magnetic separation mechanism for a chemiluminescence immunoassay system comprises a cleaning disc (1) and a cleaning rotary disc (2) which is positioned above the cleaning disc and rotates around the axis of the cleaning rotary disc, wherein a plurality of reaction cup positions are arranged on the cleaning rotary disc (2), and reaction cups are placed in the reaction cup positions, and the magnetic separation mechanism is characterized in that a plurality of groups of adsorption magnet groups (3) for offsetting magnetic beads in the reaction cups are fixedly arranged on the upper end surface of the cleaning disc (1) at the bottom end of each reaction cup, wherein the adsorption magnet groups (3) are sequentially arranged along the rotation direction of the cleaning rotary disc (2);

adsorption magnet group (3) include along washing carousel (2) direction of rotation in proper order: at least one magnet a (31) with a center line positioned at one side of the reaction cup position; and a magnet b (32) with the central line positioned at the other side of the reaction cup position.

2. The magnetic separation mechanism for a chemiluminescent immunoassay system of claim 1 wherein the magnets a (31) are sequentially adjacent to each other toward one side of the reaction cup.

3. The magnetic separation mechanism for a chemiluminescent immunoassay system according to claim 1, wherein the upper end surface of the cleaning disc (1) at the bottom end of the reaction cup is sequentially and fixedly provided with at least one magnet c (33) covering the bottom end surface of the reaction cup and a magnet d (34) with the center line positioned at the middle part of the reaction cup position, along the rotation direction of the cleaning turntable (2), wherein the magnet d (34) is positioned at the last reaction cup position of the first adsorption magnet group (3).

4. The magnetic separating mechanism for chemiluminescence immunoassay system according to claim 3, wherein the magnet c (33) is a rectangular strong magnet.

5. The magnetic separation mechanism for the chemiluminescence immunoassay system according to claim 1, wherein the upper end surface of the cleaning disc (1) at the bottom end of the reaction cup is sequentially provided with at least one magnet e (35) with the center line positioned at one side of the reaction cup position along the rotation direction of the cleaning turntable (2), the magnets e (35) are sequentially close to one side of the reaction cup position one by one, and the first magnet e (35) is positioned at the next reaction cup position of the last adsorption magnet group (3).

6. A magnetic separating mechanism for a chemiluminescence immunoassay system according to claim 5, wherein the magnet f (36) and at least one magnet g (37) located at one side corner of the reaction cup position are sequentially arranged on the upper end surface of the cleaning disc (1) at the bottom end of the reaction cup along the rotating direction of the cleaning rotary disc (2), wherein the magnet f (36) is located at the position next to the last magnet e (35).

7. The magnetic separation mechanism of claim 6, wherein the magnets f (36) and g (37) are circular magnets.

8. The magnetic separation mechanism for a chemiluminescent immunoassay system of claim 7 wherein the substrate addition site in the reaction cup is the site of the last magnet g (37) next to the reaction cup.

Technical Field

The invention relates to the field of magnetic separation, in particular to a magnetic separation mechanism for a chemiluminescence immunoassay system.

Background

In a luminescence immunoassay system, how to effectively and rapidly and effectively separate an antigen-antibody compound combined with magnetic beads from impurities is very important work, the magnetic separation in the current market generally places a magnet at the side of a reaction cup, in the cleaning process, the magnetic force is perpendicular to the wall of the reaction cup and parallel to the horizontal direction, so that the magnetic beads are adsorbed on the wall of the reaction cup, but the magnetic beads are lost due to the fact that the tension generated by the liquid level reduction caused by the self gravity of the magnetic beads and the waste liquid extraction is vertical and downward; meanwhile, in order to completely release impurities accumulated below the magnetic beads in the cleaning process, some systems adopt modes such as vibration and the like to resuspend the magnetic beads, but the structures are complex, and the measurement speed is influenced.

Disclosure of Invention

The invention aims to provide a magnetic separation mechanism for a chemiluminescence immunoassay system, wherein a magnet is arranged on a cleaning disc according to a certain rule, so that the magnet is positioned on one side of the bottom of a reaction cup during cleaning and separation, the bottom surface of the reaction cup provides good support for magnetic beads, the magnetic beads can be quickly separated during the process of cleaning reactants, magnetic bead particles are not easily sucked away by a suction needle during the cleaning process, the magnetic beads can move for a distance of about 3mm at the bottom of the cup during the cleaning process, impurities deposited below the magnetic beads can be released, and the reliability and the repeatability of a measurement result are ensured.

The purpose of the invention can be realized by the following technical scheme:

the utility model provides a chemiluminescence is magnetic separation mechanism for immunoassay system, including wasing the dish and being located washing dish top and around self axis pivoted washing carousel, it has a plurality of reaction cup position to open on the washing carousel, has placed the reaction cup in the reaction cup position, the washing dish up end fixed mounting of reaction cup bottom end has the absorption magnet group of multiunit magnetic bead in being used for the skew reaction cup, and wherein absorption magnet group installs along wasing the carousel rotation direction in proper order.

The adsorption magnet group comprises along the rotation direction of the cleaning turntable in sequence: at least one magnet a with the central line positioned at one side of the reaction cup position; and a magnet b with the central line positioned at the other side of the reaction cup position.

Furthermore, the magnets a are sequentially close to one another towards one side angle of the reaction cup position.

Further, the upper end face of the cleaning disc at the bottom end of the reaction cup is sequentially and fixedly provided with at least one magnet c covering the bottom end face of the reaction cup and a magnet d with the center line positioned in the middle of the reaction cup position, wherein the magnet d is positioned at the last reaction cup position of the first adsorption magnet group.

Further, the magnet c is a rectangular strong magnet.

Further, the upper end face of the cleaning disc at the bottom end of the reaction cup is sequentially provided with at least one magnet e with a central line positioned on one side of the reaction cup position along the rotation direction of the cleaning rotary disc, the magnets e are sequentially close to one side of the reaction cup position one by one, and the first magnet e is positioned on the next reaction cup position of the tail adsorption magnet group.

Furthermore, magnet f and at least one magnet g that is located a reaction cup position side angle are installed along wasing the carousel rotation direction in proper order to the washing dish up end of reaction cup bottom, and magnet f is located last magnet e next reaction cup position.

Further, the magnet f and the magnet g are both circular magnets.

Further, the substrate adding position in the reaction cup is the position of the last magnet g next to the reaction cup.

The invention has the beneficial effects that:

1. according to the invention, the magnetic beads in the reaction cup are adsorbed at the bottom of the reaction cup through the magnet, so that the loss of magnetism in the cleaning process is reduced;

2. according to the invention, through the deviation of the magnet mounting position of the reaction cup position, the magnetic beads move for many times in the cleaning process, and impurities precipitated under the magnetic beads are released, so that the cleaning is more thorough, the reliability and the repeatability of the measurement result are ensured, the cleaning times can be reduced, and the measurement speed is improved;

3. the invention finally adsorbs the original linearly distributed magnetic beads to the vicinity of the circle center through the round magnet to form a small magnetic bead point, which is convenient for a substrate to break the magnetic beads and obtain a good measurement result.

Drawings

The invention will be further described with reference to the accompanying drawings.

FIG. 1 is a schematic view of a magnetic separation mechanism for a chemiluminescent immunoassay system of the present invention;

fig. 2 is a partial perspective structural schematic view of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "opening," "upper," "lower," "thickness," "top," "middle," "length," "inner," "peripheral," and the like are used in an orientation or positional relationship that is merely for convenience in describing and simplifying the description, and do not indicate or imply that the referenced component or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present invention.

As shown in fig. 1 and fig. 2, a magnetic separation mechanism for a chemiluminescence immunoassay system comprises a cleaning disc 1 and a cleaning turntable 2 which is arranged above the cleaning disc and rotates around the axis of the cleaning turntable, wherein a plurality of reaction cup positions are arranged on the cleaning turntable 2, reaction cups are arranged in the reaction cup positions, a plurality of groups of adsorption magnet groups 3 used for offsetting magnetic beads in the reaction cups are fixedly arranged on the upper end surface of the cleaning disc 1 at the bottom end of each reaction cup, and the adsorption magnet groups 3 are sequentially arranged along the rotation direction of the cleaning turntable 2.

The adsorption magnet group comprises along the 2 rotating directions of the cleaning turntable in sequence: at least one magnet a31 with its center line on one side of the reaction cup position; and a magnet b32 with its centerline on the other side of the reaction cup.

The magnets a31 are sequentially close to one side corner of the reaction cup position one by one, in the embodiment of the invention, the center line of the magnet a31 is positioned at the left side of the reaction cup position, the number of the magnets a31 is two, and the magnets a31 are close to the left side corner of the reaction cup position one by one.

When in use, the cleaning turntable rotates to rotate the reaction cup with the sample to the position of the magnet a31 at one side of the position of the reaction cup, at the moment, the magnet a31 enables the magnetic beads in the reaction cup to deviate towards one side of the bottom of the reaction cup, so that impurities below the magnetic beads are released, meanwhile, the plurality of magnets a31 are sequentially shifted to one side angle of the reaction cup position to realize the multi-time shift of the magnetic beads, so that impurities below the magnetic beads are released for a plurality of times until the magnetic beads are adsorbed to the bottom angle of the reaction cup, the rotary table 2 is continuously rotated to clean one reaction cup position, the magnetic beads positioned at the bottom angle of the reaction cup are adsorbed to the other side of the bottom of the reaction cup through the magnets b32, the stroke is about 2-3 mm, and the magnetic beads move for a long distance in the liquid relative to the diameter of 1 micron of the magnetic beads, therefore, impurities precipitated under the magnetic beads can be completely released, and the reliability and repeatability of the measurement result are ensured.

The upper end face of the cleaning disc 1 at the bottom end of the reaction cup is sequentially and fixedly provided with at least one magnet c33 covering the bottom end face of the reaction cup and a magnet d34 with the central line positioned at the middle part of the reaction cup position along the rotating direction of the cleaning turntable 2, wherein the magnet d34 is positioned at the last reaction cup position of the first adsorption magnet group 3.

Among them, the magnet c33 is preferably a rectangular strong magnet.

When the magnetic beads are used, all the magnetic beads floating in the reaction cup are adsorbed to the bottom of the reaction cup by the strong adsorption of the magnet c33, and the magnetic beads are adsorbed to the middle position of the bottom of the reaction cup by the magnet d 34.

The upper end surface of the cleaning disc 1 at the bottom end of the reaction cup is sequentially provided with at least one magnet e35 with the center line positioned at one side of the reaction cup position along the rotating direction of the cleaning turntable 2, wherein the center line of the magnet e35 and the center line of the magnet a31 are positioned at the same side of the reaction cup, the magnets e35 are sequentially close to one side of the reaction cup position one by one, and the first magnet e35 is positioned at the next reaction cup position of the tail adsorption magnet group 3.

When the reaction cup rotates to the end to adsorb the magnet b32 of the magnet group 3, the magnetic beads in the reaction cup are adsorbed at the bottom end of the reaction cup close to one side of the magnet b32, the reaction cup continues to rotate, and the magnetic beads in the reaction cup are positioned at the bottom corner of the reaction cup through the magnet e 35.

The upper end face of the cleaning disc 1 at the bottom end of the reaction cup is sequentially provided with a magnet f36 and at least one magnet g37 at one side corner of the reaction cup position along the rotating direction of the cleaning turntable 2, wherein the magnet f36 is positioned at the position next to the last magnet e 35.

The magnet f36 and the magnet g37 are preferably circular magnets, the original magnetic beads which are linearly distributed are finally adsorbed near the center of a circle to form a small magnetic bead point, when the reaction cup rotates to the position of the next reaction cup of the magnet g37, the magnetic beads in the reaction cup are gathered at one side corner of the bottom end of the reaction cup, substrate adding is carried out at the moment, namely the position of the substrate adding in the reaction cup is the position of the next reaction cup of the last magnet g37, the gathered magnetic beads are washed away by the substrate, the magnetic beads are enabled to be more fully contacted with the substrate, and the measuring effect is improved.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.