阅读说明：本技术 一种便捷肠道静式替代实验装置 (Convenient intestinal static substitution experimental device ) 是由 范维林 张贵祥 黄旭升 于 2019-11-20 设计创作，主要内容包括：本发明提供一种便捷肠道静式替代实验装置,包括一个模拟人类或动物大肠形状的肠道总成,其肠腔底板连接着有若干凸起的类环状袋和类环状襞的穹状肠壁,穹状肠壁上有进气接口充入氮气,排出气口排出氧气形成无氧环境,穹状肠壁的肠腔上口配装磨边瓶盖,磨边瓶盖有进样管路和取样管路向肠道总成里灌注实验样品或者抽吸出实验样品,穹状肠壁的下边有排样下口和安装带有多种探头的下口胶塞,肠道总成周围包裹着有水浴密舱的控温水套,通过水浴进口和水浴出口连接外部的水泵和水加热器来控制温度,控温水套外表面紧密包裹隔温软垫,本发明的有益效果是：本装置可以替代活体实验低成本高效率对肠道内消化功能进行便捷快速体外静式替代实验,满足标准化的科研和应用。(The invention provides a convenient intestinal static substitution experimental device, which comprises an intestinal assembly simulating the shape of a human or animal large intestine, wherein a bottom plate of an intestinal cavity of the intestinal assembly is connected with a dome-shaped intestinal wall provided with a plurality of convex ring-like bags and ring-like folds, an air inlet port is arranged on the dome-shaped intestinal wall and filled with nitrogen, an air outlet port discharges oxygen to form an anaerobic environment, an upper opening of the intestinal cavity of the dome-shaped intestinal wall is provided with an edging bottle cap, the edging bottle cap is provided with a sample inlet pipeline and a sampling pipeline for filling an experimental sample into the intestinal assembly or sucking the experimental sample, a lower opening of the dome-shaped intestinal wall is provided with a sample discharge lower opening and a lower opening rubber plug provided with a plurality of probes, a temperature control water jacket with a water bath sealed chamber is wrapped around the intestinal assembly, an external water pump and a water heater are connected through a water bath inlet and a water bath outlet to control the temperature, and a temperature insulation cushion: the device can replace a living body experiment to carry out convenient and quick in-vitro static substitution experiment on the digestion function in the intestinal tract with low cost and high efficiency, and meets the requirements of standardized scientific research and application.)

1. The utility model provides a quiet formula of convenient intestinal replaces experimental apparatus, characterized by: comprises an intestinal assembly (26) simulating the shape of a human or animal large intestine, wherein a circular intestinal cavity bottom plate (34) at the bottommost end is arranged on the upper surface of a temperature control water jacket (4), a dome-shaped intestinal wall (3) which is seamlessly connected with the intestinal cavity bottom plate (26) and is provided with a plurality of circular ring-shaped continuous convex ring-like bags (24) and ring-like folds (23) at the connection parts is provided, an air inlet port (10) for filling nitrogen and an air outlet port (18) for discharging oxygen are arranged on the dome-shaped intestinal wall (3) so as to form an anaerobic environment in the intestinal inner cavity (25) for carrying out a substitution experiment, an edging bottle cap (16) is arranged on an upper opening (11) of the intestinal cavity at the topmost end of the dome-shaped intestinal wall (3) to seal the whole intestinal inner cavity (25), a sampling pipeline (20) and a sampling pipeline (9) are arranged on the edging bottle cap (16), and an experimental sample is filled into the intestinal assembly (26) or is pumped out for testing, observation and analysis at any time, the lower edge of the dome-shaped intestinal wall (3) is provided with a sample discharging lower opening (30) for discharging an internal sample of an experiment, a lower opening rubber plug (29) with various probes (28) can be installed to detect the experiment, the temperature control water jacket (4) with a water bath sealed cabin (6) is wrapped around the intestinal assembly (26), the temperature is controlled by a water pump and a water heater which are connected with the outside through a water bath inlet (8) and a water bath outlet (22) to ensure that the test temperature in the intestinal inner cavity (25) is always 37 ℃ or required stable temperature so as to conveniently and rapidly carry out an intestinal substitute experiment, and the temperature insulation cushion (31) tightly wrapped on the outer surface of the temperature control water jacket (4) can ensure that the loss and change of the water bath temperature can be reduced and the safety and stability of the whole device can be protected under the condition that the sight line is not affected.

2. The portable intestinal static substitution experimental device according to claim 1, which is characterized in that: the intestinal assembly (26) is an irregular thin-wall container which is made of transparent material with good heat conductivity and acid and alkali resistance and simulates the shape of a human or animal large intestine, the bottommost part of the intestinal assembly is an intestinal cavity bottom plate (34) with a circular plane base, the intestinal cavity bottom plate is arranged on the upper surface of a temperature control water jacket (4) below the intestinal cavity bottom plate, a dome-shaped intestinal wall (3) is seamlessly connected with the intestinal cavity bottom plate (34), the dome-shaped intestinal wall (3) is provided with a plurality of circular ring-shaped bags (24) with continuous bulges and annular folds (23) at the connection parts, one side of the ring-shaped bag (24) at the uppermost edge of the dome-shaped intestinal wall (3) is provided with an air inlet port (10) which can be internally and externally ventilated and is provided with a sealing cover B (19) for filling nitrogen, the other side of the ring-shaped bag (24) at the uppermost edge of the dome-shaped intestinal wall (3) is also provided with an exhaust port (18) which can be internally and externally ventilated and is provided with a sealing, so as to form the anaerobic environment in the intestinal lumen (25) and carry on the substitution experiment, it has upper mouth of intestinal lumen (11) of the edging (17) to equip with one edging bottle lid (16) with edging bottle lid (12) to seal the whole intestinal lumen (25) at the very top of the dome-shaped intestinal wall (3), there is a lower mouth of discharging (30) used for discharging the inside sample of the experiment with lower mouth edging (27) on one side of lower edge of the dome-shaped intestinal wall (3), can also install the lower mouth rubber stopper (29) with multiple probes (28), wrap up the temperature-controlled water jacket (4) that a ring can control the temperature around the whole intestinal assembly (26).

3. The portable intestinal static substitution experimental device according to claim 1, which is characterized in that: edging bottle lid (16) are by the cylindrical to the interface lid that has the intercommunication pipeline of material of acid and alkali corrosion resistance making, have round bottle lid edging (12) on edging bottle lid (16) outer edge, make edging bottle lid (16) and intestinal assembly (26) fornix form intestinal wall (3) the top one have intestinal chamber upper shed (11) of edging (17) and assemble and seal whole intestinal inner chamber (25), guarantee that the internal environment is stabilized in the experimental condition of substitution experiment requirement in intestinal inner chamber (25) of intestinal assembly (26), pass in edging bottle lid (16) the middle sampling pipeline (9) that has a less sealed flap A (14) of internal diameter, the length of sampling pipeline (9) is close the height of fornix form intestinal wall (3) of intestinal assembly (26), the sample upper shed (13) access straw of sampling pipeline (9) can aspirate the sample that substitutes the experiment at any time through sample lower mouth (35) on being close intestinal chamber bottom plate (34) and carry out and test with the sample that replaces the experiment Observation and analysis, still pass a great injection pipeline (20) that have sealed flap A (14) of internal diameter in the middle of edging bottle lid (16), the upper portion of injection pipeline (20) has injection pipe mouth (15) to insert outside container that holds the sample and pours the test sample of substitution experiment into intestinal inner chamber (25) of intestinal assembly (26) through injection pipeline (20), can both guarantee through closing up sealed flap A (14) through covering tightly on sample upper port (13) and the injection pipe mouth (15) on edging bottle lid (16) that the internal environment is stable in the experimental conditions in intestinal inner chamber (25) of intestinal assembly (26) at any time.

4. The portable intestinal static substitution experimental device according to claim 1, which is characterized in that: the temperature control water jacket (4) is a closed container made of a firm transparent heat-resistant material, the uppermost edge of the temperature control water jacket (4) is a circular sheet-shaped water jacket upper surface (21) with a round opening at the center, the center round opening of the water jacket upper surface (21) is in seamless connection with a dome-shaped intestinal wall (3) of an intestinal assembly (26), the excircle of the water jacket upper surface (21) is in seamless connection with a circle of water jacket outer wall (7) on the side wall, the lower end side wall of the water jacket outer wall (7) is provided with a round opening with the same outer diameter as a sample discharge lower opening (30) of the intestinal assembly (26) and a drainage lower opening (30) in seamless connection, the circle of the lower end of the water jacket outer wall (7) is in seamless connection with a circle of sheet-shaped water jacket lower surface (32), an inner closed cavity-shaped water bath sealed cabin (6) is formed between the temperature control water jacket (4) and the intestinal assembly (26), and one side, close to the water jacket upper surface (21), of the water jacket outer wall ( The device comprises a water bath inlet (8), a water bath inlet pipe (7), a water bath lower opening (2) which is bent and downwards connected with a water bath guide pipe (5) until the water bath lower opening is close to the lower surface (32) of the water jacket, introduced temperature-control hot water is injected into a water bath sealed chamber (6) of a temperature-control water jacket (4), a water bath outlet (22) which extends outwards and has the same shape is also arranged on the other side of the water jacket outer wall (7) close to the upper surface (21) of the water jacket, and hot water which is cooled and changed in the water bath sealed chamber (6) is led out to an external water heater to be reheated and continuously controlled in the temperature-control water jacket (4) through the water bath inlet.

5. The portable intestinal static substitution experimental device according to claim 1, which is characterized in that: separate temperature cushion (31) and be the U-shaped sheath of a transparent flexible scalable insulation material preparation, separate temperature cushion (31) bottom and be the same cushion collet (33) of appearance and temperature control water jacket (4) water jacket below (32) shape, cushion collet (33) excircle connection round internal diameter and temperature control water jacket (4) water jacket outer wall (7) external diameter the same cushion lateral wall (1), cushion lateral wall (1) lower part is opened has and is led out from with the round mouth that the stock form end opening (30) external diameter the same of intestinal assembly (26) lets stock form end opening (30) from it, cushion collet (33) paste tightly below the water jacket (32) of temperature control water jacket (4), same cushion lateral wall (1) paste tightly outside water jacket outer wall (7) of temperature control water jacket (4), whole separate temperature cushion (31) paste tightly and form a inseparable parcel to it in the outside of temperature control water jacket (4), because separate temperature cushion (31) so can not influence the sight by the transparent flexible scalable insulation material preparation The temperature control water jacket (4) can reduce the loss and the change of the water bath temperature and simultaneously protect the safety and the stability of the whole device.

Technical Field

The invention relates to a convenient intestinal static substitution experimental device.

Background

As the research of digestive absorption is needed in more and more scientific researches in natural science researches such as food nutrition, functional active substance metabolism research, drug release power research, probiotics and prebiotics, food toxicology research, animal nutrition and feed research and the like for predicting or evaluating the digestibility, bioavailability, release kinetic characteristics, structural change and the like of compounds, in the digestive absorption experiments of the prior scientific researches, the human or animal living bodies are often adopted for experiments, firstly the ethical morals are violated, secondly, because the living body experiments have great individual differences, and the intestinal environment of the living bodies in the experimental process can not completely ensure the normal environment, and the experimental samples taken out for the scientific researches after the experiments can not correctly reflect the digestive absorption condition of the intestinal tract and can not ensure the repeatability and the accuracy of the experiments, influences the conclusion of scientific research to a certain extent and can not meet the requirements of the current scientific research, thereby restricting the development of related scientific research. Therefore, the development of a convenient intestinal tract static type substitution experimental device for simulating the shape and the internal environmental conditions of the large intestine of a human or an animal and completely substituting the living body for carrying out the intestinal tract digestion function experiment safely and stably becomes the current urgent need.

Disclosure of Invention

In order to solve the problems, the invention aims to provide a convenient intestinal static substitution experimental device which is used for simulating the shape and the internal environmental conditions of the large intestine of a human or an animal, completely substituting the living body for safely and stably carrying out an intestinal digestion function experiment and improving the repeatability and the accuracy of the experiment.

In order to achieve the above objects, the present invention provides a portable intestinal tract static substitution experimental device, which comprises an intestinal tract assembly simulating the shape of human or animal large intestine, wherein a circular intestinal cavity bottom plate at the bottom end is arranged on the upper surface of a temperature control water jacket, a dome-shaped intestinal wall which is seamlessly connected with the intestinal cavity bottom plate and is provided with a plurality of circular continuous convex ring-like bags and ring-like folds at the connection parts is provided, an air inlet port for filling nitrogen and an air outlet port for discharging oxygen are arranged on the dome-shaped intestinal wall so as to form an anaerobic environment in the intestinal tract inner cavity for substitution experiments, an edging bottle cap is arranged at the upper port of the intestinal cavity at the top end of the dome-shaped intestinal wall to seal the whole intestinal tract inner cavity, the edging bottle cap penetrates through a sampling pipeline and an experiment sample feeding pipeline to the intestinal tract assembly at any time or sucking the experiment sample for testing and observation analysis, and an internal sample probe which is provided with a sample discharging lower port for discharging experiments is arranged at the lower edge of the dome-shaped intestinal wall The lower mouthful plug detect the experiment, and the intestinal assembly is surrounding to be wrapped up the accuse temperature water jacket that has the water bath cabin of sealing, and the outside water pump and the water heater of water bath import and water bath exit linkage come the controlled temperature to be used for guaranteeing that the test temperature of intestinal intracavity is 37 degrees centigrade or the stable temperature that requires all the time so that carry out convenient quick intestinal substitution experiment. The temperature-insulating soft cushion tightly wrapped on the outer surface of the temperature-control water jacket ensures that the temperature-control water jacket can reduce the loss and change of the water bath temperature and simultaneously protects the whole device to be safe and stable under the condition of not influencing the sight.

The invention has the beneficial effects that: the device simulates the in-vivo digestion and absorption condition under the in-vitro condition by simulating the shape of the large intestine of a human or an animal and the internal environment condition, and carries out convenient and quick in-vitro static substitution experiments on the digestion function in the intestinal tract with low cost and high efficiency, thereby meeting the standardized scientific research and application.

Drawings

FIG. 1 is a schematic diagram of the structure of the present invention.

In the figure:

1. side wall of soft pad 2, lower mouth of water bath 3, dome-shaped intestinal wall 4, temperature-controlled water jacket 5, water bath leading pipe 6, water bath sealed chamber 7, water jacket outer wall

8. A water bath inlet 9, a sampling pipeline 10, an air inlet connector 11, an upper opening of an intestinal cavity 12, a bottle cap edge grinding 13 and an upper sampling opening

14. Sealing cover A15, injection pipe port 16, edge-polished bottle cap 17, upper port edge-polished 18, air exhaust port 19 and sealing cover B

20. Sample introduction pipeline 21, water jacket upper surface 22, water bath outlet 23, similar annular plica 24, similar annular bag 25, intestinal tract inner cavity

26. Intestinal assembly 27, lower opening edge grinding 28, various probes 29, lower opening rubber plug 30, sample discharging lower opening 31 and heat insulation cushion

32. The lower part 33 of the water jacket, the soft pad bottom support 34, the intestinal cavity bottom plate 35 and the lower sampling opening.

Detailed Description

The invention is described with reference to the accompanying drawings and examples.

As shown in the attached drawings, the invention provides a convenient intestinal static replacement experimental device, which comprises an intestinal assembly 26 simulating the shape of a human or animal large intestine, wherein the intestinal assembly 26 is an irregular thin-wall container which is made of transparent material with good heat conductivity and acid and alkali corrosion resistance and is made of an irregular thin-wall container simulating the shape of the human or animal large intestine, an intestinal cavity bottom plate 34 of which the bottommost part is a circular plane base is arranged on the upper surface of a temperature-controlled water jacket 4 below the intestinal assembly, a dome-shaped intestinal wall 3 is seamlessly connected with the intestinal cavity bottom plate 34, a plurality of circular ring-shaped bags 24 which are continuously raised and connected with the dome-shaped intestinal wall 3 are arranged on the dome-shaped intestinal wall 3, an air inlet port 10 which can be internally and externally ventilated and is provided with a sealing cover B19 is arranged on one side of the ring-shaped bag 24 on the uppermost edge of the dome-shaped intestinal wall 3 for filling nitrogen, an exhaust port 18 which can be internally and externally ventilated and is provided with a sealing cover B19 is arranged on the other side of the ring-shaped bag 24 on, so as to form an anaerobic environment in the intestinal canal inner cavity 25 for carrying out a substitution experiment, the uppermost end of the dome-shaped intestinal wall 3 is provided with an intestinal canal upper opening 11 with an upper opening edging 17, an edging bottle cap 16 with a bottle cap edging 12 is assembled to seal the whole intestinal canal inner cavity 25, the edging bottle cap 16 is a cylindrical butt joint cover which is made of an acid-resistant and alkali-resistant material and is provided with a communicating pipeline, the outer edge of the edging bottle cap 16 is provided with a circle of bottle cap edging 12, so that the edging bottle cap 16 and the intestinal canal upper opening 11 with the upper opening edging 17 at the uppermost end of the dome-shaped intestinal wall 3 of the intestinal canal assembly 26 are assembled to seal the whole intestinal canal inner cavity 25, the internal environment in the intestinal canal inner cavity 25 of the intestinal canal assembly 26 is ensured to be stable in the experiment condition required by the substitution experiment, a sampling pipeline 9 with a sealing opening cover A14 with a smaller inner diameter passes through the middle of the edging bottle cap 16, the length of the sampling, a sampling upper port 13 of the sampling pipeline 9 is connected with a suction pipe, a sample of a substitution experiment can be sucked at any time through a sampling lower port 35 close to the intestinal cavity bottom plate 34 for assay and observation analysis, a sampling pipeline 20 with a sealing opening cover A14 and a larger inner diameter passes through the middle of the edging bottle cap 16, a sampling pipe port 15 arranged at the upper part of the sampling pipeline 20 is connected with a container for externally containing the sample, the sample of the substitution experiment is filled into an intestinal cavity 25 of the intestinal assembly 26 through the sampling pipeline 20, and the sampling upper port 13 and the sampling pipe port 15 on the edging bottle cap 16 can be tightly covered with the sealing opening cover A14 at any time to ensure that the internal environment in the intestinal cavity 25 of the intestinal assembly 26 is stabilized in an experimental condition; a sample discharging lower opening 30 with a lower opening edge grinding 27 is arranged at one side of the lower edge of the dome-shaped intestinal wall 3 and used for discharging internal samples of experiments, a lower opening rubber plug 29 with various probes 28 can be installed, and a circle of temperature control water jacket 4 capable of controlling the temperature is wrapped around the whole intestinal assembly 26; the temperature control water jacket 4 is a closed container made of firm transparent heat-resisting material, the uppermost edge of the temperature control water jacket 4 is a circular sheet-shaped water jacket upper surface 21 with a round opening at the center, the central round opening of the water jacket upper surface 21 is seamlessly connected with the dome-shaped intestinal wall 3 of the intestinal assembly 26, the excircle of the water jacket upper surface 21 is seamlessly connected with a circle of water jacket outer wall 7 on the side wall, the side wall at the lower end of the water jacket outer wall 7 is provided with a round opening with the same outer diameter as the drainage lower opening 30 of the intestinal assembly 26 and is seamlessly connected with the drainage lower opening 30, the circle at the lower end of the water jacket outer wall 7 is seamlessly connected with the wafer-shaped water jacket lower surface 32, an inner closed cavity-shaped water bath sealed cabin 6 is formed between the temperature control water jacket 4 and the intestinal assembly 26, one side of the water jacket outer wall 7 close to the water jacket upper surface 21 is provided with an outwardly extending thin tubular water bath inlet 8 connected with an external water pump and a water heater, the water bath inlet 2, injecting the introduced temperature-controlled hot water into a water bath sealed chamber 6 of the temperature-controlled water jacket 4, and leading the hot water which is cooled and changed in the water bath sealed chamber 6 out to an external water heater for reheating through a water pump and a water bath inlet 8 to continuously control the temperature of the water bath in the temperature-controlled water jacket 4, wherein the other side of the outer wall 7 of the water jacket, which is close to the upper surface 21 of the water jacket, is also provided with a water bath outlet 22 which extends outwards and has the same shape; the temperature control water jacket 4 is connected with an external water pump and a water heater through a water bath inlet 8 and a water bath outlet 22 to control the temperature so as to ensure that the test temperature in the intestinal tract inner cavity 25 is always 37 ℃ or the required stable temperature so as to conveniently and rapidly carry out an intestinal tract substitution experiment; the outer surface of the temperature control water jacket 4 is tightly wrapped by the temperature insulation cushion 31, the temperature insulation cushion 31 is a U-shaped sheath made of transparent soft telescopic heat insulation materials, the bottom of the temperature insulation cushion 31 is a cushion base 33 with the same shape as the lower surface 32 of the temperature control water jacket 4, the outer circle of the cushion base 33 is connected with a cushion side wall 1 with the same inner diameter as the outer diameter of the water jacket outer wall 7 of the temperature control water jacket 4, the lower part of the cushion side wall 1 is provided with a round opening with the same outer diameter as the lower opening 30 of the sample arrangement of the intestinal assembly 26, so that the lower opening 30 of the sample arrangement is led out from the round opening, the cushion base 33 is tightly attached to the lower side of the lower surface 32 of the water jacket of the temperature control water jacket 4, the cushion side wall 1 is tightly attached to the outer side wall 7 of the temperature control water jacket 4, the whole temperature insulation cushion 31 is tightly attached to the outer side of the temperature control water jacket 4 to form a tight wrapping, and the temperature insulation cushion 31 Meanwhile, the whole device is well protected to be safe and stable.