阅读说明：本技术 一种羽毛粉制备氨基酸水溶肥的工艺方法 (Process method for preparing amino acid water-soluble fertilizer from feather meal ) 是由 黄国林 洪家胜 于 2019-11-13 设计创作，主要内容包括：本发明涉及水溶肥制备技术领域,且本发明公开了一种羽毛粉制备氨基酸水溶肥的工艺方法,包括以下步骤,首先准备以实验室筛选出的产角蛋白酶的地衣芽孢杆菌为出发菌株,以羽毛粉为原料,通过单因子实验研究含水量、接种量、发酵温度、加酶量等对水解度的影响。该羽毛粉制备氨基酸水溶肥的工艺方法,以羽毛粉为原料,再通过发酵和酶解相结合的方法进行制备氨基酸水溶肥,不仅提高羽毛废弃物的加工利用,减少羽毛废弃物对环境造成污染,降低羽毛废弃物传播疾病的概率,还开发出一种新的氨基酸液态肥的制备方法,利用羽毛粉内部含有的在85%以上的粗蛋白含量,包含的17种氨基酸,减少和降低在施用氨基酸水溶肥料而影响土壤的情况发生。(The invention relates to the technical field of water-soluble fertilizer preparation, and discloses a process method for preparing amino acid water-soluble fertilizer from feather meal. The process method for preparing the amino acid water-soluble fertilizer from the feather powder comprises the steps of taking the feather powder as a raw material, preparing the amino acid water-soluble fertilizer by a method combining fermentation and enzymolysis, improving the processing and utilization of feather waste, reducing the pollution of the feather waste to the environment, and reducing the probability of disease spread of the feather waste, and developing a novel preparation method of the amino acid liquid fertilizer.)

1. A process method for preparing an amino acid water-soluble fertilizer from feather meal is characterized by comprising the following steps:

s1: firstly, preparing a bacillus licheniformis which is screened out in a laboratory and produces keratinase as an original strain, and feather meal as a raw material;

s2: the influence of water content, inoculation amount, fermentation temperature, enzyme addition amount and the like on the hydrolysis degree is researched through a single-factor experiment, and an orthogonal experiment is used for optimization on the basis of the single-factor experiment, so that the prepared feather meal is fermented;

s3: selecting common enzymes such as neutral protease, alkaline protease, papain and pancreatin, under the condition of consistent factors such as feed-liquid ratio, enzyme addition amount and enzymolysis time, screening out the most suitable enzyme by comprehensively comparing indexes such as amino nitrogen content and soluble protein content of enzymolysis liquid after enzymolysis, and carrying out liquid enzymolysis with the feather powder;

s4: selecting the most appropriate enzymolysis liquid from the third step as a raw material, adding the trace elements of manganese, zinc and copper, and determining the optimal chelation process conditions by researching the influence of factors such as pH, temperature, time and the like on the chelation rate of the trace elements for many times;

s5: then measuring the content of free amino acid, the composition analysis of amino acid, the content of trace elements, the content of water-insoluble substances and the like of the prepared amino acid water-soluble fertilizer, and laying a foundation for the establishment of enterprise standards of the amino acid water-soluble fertilizer;

s6: a field plot experiment is carried out, the influence of the amino acid water-soluble fertilizer on the yield and the growth speed of vegetables such as hot peppers, flowering cabbages and the like is analyzed, the field effect of the amino acid water-soluble fertilizer is determined, and the application procedure of the amino acid water-soluble fertilizer is determined;

s7: the preparation process of the functional protein is determined through the preparation steps, the parameter conditions and the matched equipment of the pilot plant production are researched, the industrial production is carried out on the basis of the pilot plant production process, and the enterprise standard of the product is formulated.

2. The process method for preparing the amino acid water-soluble fertilizer from the feather meal as claimed in claim 1, wherein the enzyme research in the third step further comprises the step of researching the influence of factors such as enzyme adding amount, enzymolysis time, feed-liquid ratio and pH on enzymolysis results, and performing an orthogonal test under the results to determine the optimal enzymolysis process conditions.

3. The process for preparing amino acid water-soluble fertilizer from feather meal as claimed in claim 1, wherein the fermented feather meal described in step three is fermented feather meal without drying treatment.

4. The process for preparing amino acid water-soluble fertilizer from feather meal as claimed in claim 1, wherein the enzymes in step three are screened while adjusting temperature and pH value to the optimum conditions for each enzyme.

5. The process for preparing amino acid water-soluble fertilizer from feather meal as claimed in claim 1, wherein the feather meal selected in the first step has crude protein content of more than 85%, contains 17 amino acids, and has complete essential amino acids, and animal feathers are prepared by high temperature and high pressure hydrolysis.

Technical Field

The invention relates to the technical field of water-soluble fertilizer preparation, in particular to a process method for preparing amino acid water-soluble fertilizer from feather meal.

Background

The amino acid water-soluble fertilizer is a new generation amino acid liquid fertilizer which is produced by taking agricultural and sideline product resources as main raw materials and utilizing the biological fermentation technology and the film concentration technology and integrates the characteristics of multiple functions, high technology, environmental protection and the like, and has the advantages of stimulating the growth of plants and improving the yield of crops; the quality of crops is improved, and the content of protein and vitamin is increased; promoting the activation of soil microbial activity, enhancing crop stress resistance and the like. The large-scale application of the fertilizer plays an important role in the development of agriculture in China.

The existing amino acid water-soluble fertilizer can not only cause soil hardening, acidification, organic matter reduction and water eutrophication, but also seriously affect the ecological environment when being applied for a long time and applied in an excessive and unscientific way.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a process method for preparing an amino acid water-soluble fertilizer by feather meal, which solves the problems that the existing amino acid water-soluble fertilizer is applied for a long time and is applied in an excessive and unscientific way, so that the soil is hardened and acidified, organic matters are reduced, water eutrophication is caused, and the ecological environment is seriously influenced.

(II) technical scheme

In order to achieve the purpose, the invention provides the following technical scheme: a process method for preparing an amino acid water-soluble fertilizer from feather meal comprises the following steps:

s1: firstly, bacillus licheniformis which is screened out in a laboratory and produces keratinase is taken as an original strain, and feather meal is taken as a raw material.

S2: the influence of water content, inoculation amount, fermentation temperature, enzyme addition amount and the like on the hydrolysis degree is researched through a single-factor experiment, and the prepared feather meal is fermented by using an orthogonal experiment on the basis of the single-factor experiment.

S3: the above fermented feather powder is used as raw material, and the neutral protease, alkaline protease, papain and pancreatin are selected, under the condition of consistent factors of material-liquid ratio, enzyme adding quantity and enzymolysis time, the most suitable enzyme is selected by comprehensively comparing indexes of amino nitrogen content, soluble protein content and the like of the enzymolysis liquid after enzymolysis, and is subjected to liquid enzymolysis together with the feather powder.

S4: and (3) selecting the most appropriate enzymolysis liquid from the third step as a raw material, adding the trace elements of manganese, zinc and copper, and determining the optimal chelation process conditions by researching the influence of factors such as pH, temperature, time and the like on the chelation rate of the trace elements for many times.

S5: and then the content of free amino acid, the composition analysis of amino acid, the content of trace elements, the content of water-insoluble substances and the like in the prepared amino acid water-soluble fertilizer are determined, and a foundation is laid for the establishment of the enterprise standard of the amino acid water-soluble fertilizer.

S6: a field plot experiment is carried out, the influence of the amino acid water-soluble fertilizer on the yield and the growth speed of vegetables such as hot peppers, flowering cabbage and the like is analyzed, the field effect of the amino acid water-soluble fertilizer is determined, and the application rule of the amino acid water-soluble fertilizer is determined.

S7: the preparation process of the functional protein is determined through the preparation steps, the parameter conditions and the matched equipment of the pilot plant production are researched, the industrial production is carried out on the basis of the pilot plant production process, and the enterprise standard of the product is formulated.

Preferably, the research on the enzyme in the third step further includes the research on the influence of factors such as enzyme adding amount, enzymolysis time, feed-liquid ratio, pH and the like on the enzymolysis result. And performing orthogonal test under the result to determine the optimal enzymolysis process condition.

Preferably, the fermented feather powder described in the third step is fermented feather powder which is not subjected to drying treatment.

Preferably, the enzymes in step three are screened while adjusting the temperature and pH to the optimum conditions for each enzyme.

Preferably, the crude protein content of the feather powder selected in the step one is more than 85%, the feather powder comprises 17 amino acids, essential amino acids are complete, and animal feathers are prepared by a high-temperature high-pressure hydrolysis method.

(III) advantageous effects

Compared with the prior art, the invention provides a process method for preparing an amino acid water-soluble fertilizer from feather meal, which has the following beneficial effects:

1. the process method for preparing the amino acid water-soluble fertilizer from the feather powder comprises the steps of taking the feather powder as a raw material, preparing the amino acid water-soluble fertilizer by a method combining fermentation and enzymolysis, improving the processing and utilization of feather wastes, reducing the pollution of the feather wastes to the environment, and reducing the probability of disease spreading of the feather wastes, and developing a novel preparation method of the amino acid liquid fertilizer.

2. The process method for preparing the amino acid water-soluble fertilizer from the feather meal integrates the advantages of solid fermentation and liquid enzymolysis by adopting a method of combining the solid fermentation with the liquid enzymolysis, and fully hydrolyzes the feather meal by utilizing the synergistic action of keratinase and protease.

3. The process method for preparing the amino acid water-soluble fertilizer from the feather powder determines the final chelating process of the trace elements and the enzymolysis liquid by integrating the chelating rate of the trace elements and the enzymolysis liquid and the requirements of the trace elements and crops on the trace elements by the trace element amino acid water-soluble fertilizer.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.