阅读说明：本技术 一种桡足类样品鉴定前的整形方法 (Reshaping method before copepod sample identification ) 是由 周超 金扬湖 于 2019-08-02 设计创作，主要内容包括：本发明公开了一种桡足类样品鉴定前的整形方法,涉及桡足类的实验方法技术领域；步骤如下：步骤a：将需要整形的桡足类放入中性福尔马林溶液中,存放于阴凉干燥处；步骤b：过滤得到桡足类；步骤c：将过滤得到的桡足类重新放入新的中性福尔马林溶液中,使水分子进入桡足类机体组织的缝隙内,从而使桡足类的体态复原。本发明能将蜷缩的桡足类体态复原,方便观察。(The invention discloses a shaping method before identification of a copepod sample, and relates to the technical field of experimental methods of copepods; the method comprises the following steps: step a: putting copepods to be shaped into neutral formalin solution, and storing in a cool and dry place; step b: filtering to obtain copepods; step c: and putting the filtered copepods into a new neutral formalin solution again, so that water molecules enter gaps of tissues of copepods, thereby restoring the physique of copepods. The invention can recover the physical state of the crouched copepods, and is convenient to observe.)

1. A reshaping method before identification of a copepod sample is characterized by comprising the following steps:

step a: putting copepods to be shaped into neutral formalin solution, and storing in a cool and dry place;

step b: filtering to obtain copepods;

step c: and putting the filtered copepods into a new neutral formalin solution again, so that water molecules enter gaps of tissues of copepods, thereby restoring the physique of copepods.

2. The method according to claim 1, wherein the neutral formalin solution is prepared from 100ml of formaldehyde, 900ml of distilled water, 4 g of sodium dihydrogen phosphate and 6.5 g of sodium monohydrogen phosphate.

3. The method of claim 1, wherein in step a, the sample is stored for 30 days.

4. The method of claim 1, wherein step c is performed at 40 ℃.

5. The method of claim 4, wherein the copepod sample is placed in a neutral formalin solution on a shaker and shaken for 50 minutes at 60 r/min.

6. The method of claim 1, wherein step b is performed by 90 mesh bolting.

7. The method for shaping a copepod sample before its identification as claimed in claim 1, 2, 3, 4 or 5, wherein the filtering device is used in step b, the filtering device comprises a base, a filtering cup is fixed on top of the base, a bolting silk is arranged on the bottom of the inner side of the filtering cup, a flow guiding pipe is connected to the bottom of the filtering cup, a support is fixedly connected to the base, a sliding block is slidably connected to the support, one end of the flow guiding pipe away from the filtering cup is fixedly connected to the sliding block, a water cup is arranged below one end of the flow guiding pipe away from the filtering cup, the inner diameter of the water cup is consistent with the inner diameter of the filtering cup, a floating ball is arranged in the water cup, the support is rotatably connected to a guiding wheel above the sliding block, a support is fixedly connected to the support, a second guiding wheel is rotatably connected to the support above the floating ball, one end of the stay wire, which is far away from the slide block, bypasses the guide wheel and the second guide wheel in sequence and is connected to the floating ball.

Technical Field

The invention belongs to the technical field of experimental methods of copepods, and particularly relates to a shaping method before copepod sample identification.

Background

Copepods belonging to the phylum Arthropoda, Crustacea, and copepoda, there are a total of 7500 copepods worldwide, of which about 4500 species have been identified as marine copepods. Copepods are large in number, wide in distribution, short in growth cycle, small in size and difficult in species identification. Along with the vigorous promotion of water ecological civilization construction in China, the water quality condition of a water body is objectively evaluated, and the copepods identification work is particularly important. The crunch of the appendages and the antennae of the copepods after long-time preservation of the fixative is not favorable for observation, and a pretreatment recovery method is urgently needed.

Disclosure of Invention

The invention aims to overcome the defect that the short-time storage of copepods is not beneficial to observation, and provides a shaping method before copepods sample identification, which can restore the coiled copepods and is convenient to observe.

In order to achieve the purpose, the invention adopts the following technical scheme:

a shaping method before identification of a copepod sample comprises the following steps: step a: putting copepods to be shaped into neutral formalin solution, and storing in a cool and dry place; step b: filtering to obtain copepods; step c: and putting the filtered copepods into a new neutral formalin solution again, so that water molecules enter gaps of tissues of copepods, thereby restoring the physique of copepods. Storing in shady and dry place to reduce oxidation of neutral formalin solution, slowly adjusting acidity and alkalinity of copepods, storing for one month, and then replacing with new neutral formalin solution to make water molecules enter into gaps of copepods organism tissue, thereby restoring the physique of copepods.

Preferably, the neutral formalin solution is prepared from 100ml of formaldehyde, 900ml of distilled water, 4 g of sodium dihydrogen phosphate and 6.5 g of sodium monohydrogen phosphate. The preparation is easy.

Preferably, in step a, the storage time is 30 d.

Preferably, step c is carried out at 40 ℃. Accelerating recovery in high temperature environment.

Preferably, the neutral formalin solution placed in copepod is placed on a shaker and shaken for 50 minutes at 60r/min on the shaker. In the rocking state, the recovery is further accelerated.

Preferably, step b is performed by 90 mesh bolting. The 90-mesh bolting silk can filter water fleas.

Preferably, the filtering device is adopted for filtering in the step b and comprises a base, a filtering cup is fixed above the base, a bolting silk is arranged at the bottom of the inner side of the filtering cup, a flow guide pipe is connected to the bottom of the filtering cup, a support is fixedly connected to the base, a sliding block is slidably connected to the support, one end, away from the filtering cup, of the flow guide pipe is fixedly connected to the sliding block, a water cup is arranged below one end, away from the filtering cup, of the flow guide pipe, the inner diameter of the water cup is consistent with that of the filtering cup, a floating ball is arranged in the water cup, the support is rotatably connected to the upper portion of the sliding block and is fixedly connected to the support, a second guide wheel is rotatably connected to the upper portion of the floating ball, a stay wire is connected to the sliding block, and one end, away from the. The traditional filtering device is adopted for filtering, after the water fleas abut against the bolting silk, the neutral formalin solution above the water fleas can extrude the water fleas, so that the water fleas can be seriously extruded and damaged, and the later-period observation is inconvenient; the filter device can not destroy water fleas.

The invention has the beneficial effects that: the shaping method before the copepod sample identification can restore the body state of the crouched copepods and is convenient to observe. In addition, the copepods are not easily crushed by the filter device.

Drawings

Fig. 1 is a schematic view of a filtration apparatus.

In the figure: the device comprises a filter cup 1, a bolting silk 2, a base 3, a draft tube 4, a support 5, a slide block 6, a water cup 7, a guide wheel 8, a floating ball 9, a stay wire 10, a bracket 11, a second guide wheel 12 and a clamp 13.

Detailed Description

The invention is explained in further detail below with reference to the figures and the detailed description: