阅读说明：本技术 离体器官灌注滤过系统 (Isolated organ perfusion filtration system ) 是由 陈静瑜 卫栋 于 2019-08-26 设计创作，主要内容包括：本申请涉及一种离体器官灌注滤过系统,包括：储液器；储液器的输入端用于与离体器官灌注系统的出液口贯通连接；滤过子系统,滤过子系统的输入端与储液器的输出端贯通连接,输出端用于与离体器官灌注系统的进液口贯通连接；补液子系统,补液子系统用于与离体器官灌注系统的进液口贯通连接；控制子系统,控制子系统分别与滤过子系统、补液子系统通信连接；其中,控制子系统用于驱动滤过子系统；控制子系统还用于处理采集到的储液器中待过滤灌注液的液位数据,得到补充液的补充数据,以及基于补充数据,驱动补液子系统向离体器官灌注系统传输补充液。本申请能够实现系统的灌注补液平衡,提高了离体器官的灌注效果。(The present application relates to an ex vivo organ perfusion filtration system comprising: a reservoir; the input end of the liquid storage device is used for being communicated with a liquid outlet of the isolated organ perfusion system; the input end of the filtering subsystem is communicated with the output end of the liquid storage device, and the output end of the filtering subsystem is communicated with the liquid inlet of the isolated organ perfusion system; the fluid infusion subsystem is used for being communicated with a fluid inlet of the isolated organ perfusion system; the control subsystem is respectively in communication connection with the filtering subsystem and the fluid infusion subsystem; wherein the control subsystem is used for driving the filtering subsystem; the control subsystem is also used for processing the acquired liquid level data of the perfusate to be filtered in the liquid storage device to obtain the supplement data of the supplement liquid, and driving the supplement liquid subsystem to transmit the supplement liquid to the isolated organ perfusion system based on the supplement data. The application can realize the perfusion fluid infusion balance of the system and improve the perfusion effect of isolated organs.)

1. An ex vivo organ perfusion filtration system, comprising:

the input end of the liquid storage device is used for being communicated with the liquid outlet of the isolated organ perfusion system; the liquid storage device stores perfusate to be filtered of the isolated organ perfusion system;

the input end of the filtering subsystem is communicated with the output end of the liquid storage device, and the output end of the filtering subsystem is communicated with the liquid inlet of the isolated organ perfusion system; the filtration subsystem filters the perfusate to be filtered;

the fluid infusion subsystem is used for being communicated with a fluid inlet of the isolated organ perfusion system; the fluid infusion subsystem transmits a supplementary fluid to the isolated organ perfusion system;

the control subsystem is in communication connection with the filtering subsystem and the fluid infusion subsystem respectively;

wherein the control subsystem is used for driving the filtering subsystem; the control subsystem is further used for processing the acquired liquid level data of the perfusate to be filtered in the liquid storage device, obtaining supplement data of the supplement liquid, and driving the supplement liquid subsystem to transmit the supplement liquid to the isolated organ perfusion system based on the supplement data.

2. The isolated organ perfusion filtration system of claim 1, wherein the control subsystem comprises a master control device and a first level sensor disposed in the reservoir;

the first liquid level sensor is in communication connection with the main control equipment.

3. The ex vivo organ perfusion filtration system of claim 1, further comprising a waste fluid collection device;

and the waste liquid collecting equipment is communicated with the waste liquid discharge end of the filtering subsystem.

4. The isolated organ perfusion filtration system of claim 3, wherein the waste fluid collection apparatus comprises a waste fluid collection device connected through a discharge end of the filtration subsystem, and a second fluid level sensor disposed in the waste fluid collection device;

the second level sensor is in communication with the control subsystem.

5. The ex vivo organ perfusion filtration system of claim 3, wherein the filtration subsystem comprises a first tubing assembly, a second tubing assembly, a filtration device, and a power pump communicatively connected to the control subsystem;

the power pump is connected between the liquid reservoir and the filtering equipment in a penetrating way through the first pipeline assembly; the filtering equipment is communicated with the isolated organ perfusion system through the second pipeline assembly.

6. The ex vivo organ perfusion filtration system of claim 5, wherein the filtration subsystem further comprises a pressure sensor disposed in the first tubing assembly;

the pressure sensor is in communication with the control subsystem.

7. The isolated organ perfusion filtration system of claim 5, wherein the filtration device comprises a waste fluid cavity in communication with the waste fluid collection device, a perfusate cavity in communication with the isolated organ perfusion system, and a semi-permeable membrane device in communication between the perfusate cavity and the waste fluid cavity.

8. The isolated organ perfusion filtration system according to claim 1, wherein the fluid infusion subsystem comprises a fluid storage part, an infusion pump connected between a fluid inlet of the isolated organ perfusion system and the fluid storage part, and a pinch valve arranged between the fluid storage part and the infusion pump;

the infusion pump is in communication connection with the control subsystem.

9. The isolated organ perfusion filtration system of claim 8, wherein the fluid replacement subsystem further comprises a third fluid level sensor disposed in the fluid reservoir;

the third liquid level sensor is in communication with the control subsystem.

10. The ex vivo organ perfusion filtration system of claim 9, further comprising an alarm device connected to the control subsystem.

11. The ex vivo organ perfusion filtration system of any one of claims 1-10, further comprising a server communicatively coupled to the control subsystem, and a mobile monitoring device communicatively coupled to the control subsystem and the server, respectively.

Technical Field

The application relates to the technical field of medical instruments, in particular to an isolated organ perfusion filtration system.

Background

In organ transplantation, the development of organ transplantation is severely limited by the shortage of donor organs. At present, organs used for transplantation are mainly well-conditioned donor organs, a large number of marginal donor organs are generally abandoned, and development of ex vivo Mechanical Perfusion (MP) technology makes it possible to apply the marginal donor organs in clinics.

However, long-time isolated organ perfusion can make isolated organ produce a large amount of metabolites, such as water, lactic acid, inorganic salts and urea, and these metabolites stay in isolated organ's perfusate, can follow extracorporeal circulation pipe-line system reentry isolated organ in, when these metabolites accumulate when a certain amount, if can not discharge in time, can cause isolated organ's further damage, can't save marginal donor organ to the possibility of transplantation has been reduced.

In the implementation process, the inventor finds that at least the following problems exist in the conventional technology: the traditional perfusate liquid changing process of the isolated organ is only simple replacement, the price of the perfusate is often expensive, great waste is caused, the operation is complex, and meanwhile, the pollution is easy.

Disclosure of Invention

Based on this, it is necessary to provide an isolated organ perfusion filtration system to the traditional perfusate liquid change process to isolated organ, and the liquid change is only simply replaced the perfusate, causes very big waste and complex operation, takes place secondary pollution's problem easily.

In order to achieve the above object, an embodiment of the present invention provides an isolated organ perfusion filtration system, including:

the input end of the liquid storage device is used for being communicated with a liquid outlet of the isolated organ perfusion system; the liquid storage device stores perfusate to be filtered of the isolated organ perfusion system;

the input end of the filtering subsystem is communicated with the output end of the liquid storage device, and the output end of the filtering subsystem is communicated with the liquid inlet of the isolated organ perfusion system; the filtration subsystem filters the perfusate to be filtered;

the fluid infusion subsystem is used for being communicated with a fluid inlet of the isolated organ perfusion system; the fluid infusion subsystem transmits the supplementary fluid to the isolated organ perfusion system;

the control subsystem is respectively in communication connection with the filtering subsystem and the fluid infusion subsystem;

wherein the control subsystem is used for driving the filtering subsystem; the control subsystem is also used for processing the acquired liquid level data of the perfusate to be filtered in the liquid storage device to obtain the supplement data of the supplement liquid, and driving the supplement liquid subsystem to transmit the supplement liquid to the isolated organ perfusion system based on the supplement data.

In one embodiment, the control subsystem comprises a main control device and a first liquid level sensor arranged on the liquid storage device;

the first liquid level sensor is in communication connection with the main control device.

In one embodiment, the device further comprises a waste liquid collecting device; the waste liquid collecting device is communicated with the waste liquid discharge end of the filtering subsystem.

In one embodiment, the waste liquid collecting device comprises a waste liquid collecting device which is communicated with the discharge end of the filtering subsystem, and a second liquid level sensor which is arranged on the waste liquid collecting device;

the second level sensor is in communication with the control subsystem.

In one embodiment, the filtration subsystem comprises a first tubing assembly, a second tubing assembly, a filtration device, and a power pump communicatively coupled to the control subsystem;

the power pump is communicated and connected between the liquid reservoir and the filtering equipment through a first pipeline assembly; the filtering equipment is communicated with the isolated organ perfusion system through a second pipeline assembly.

In one embodiment, the filtration subsystem further comprises a pressure sensor disposed in the first tube assembly;

the pressure sensor is in communication with the control subsystem.

In one embodiment, the filtering device comprises a waste liquid cavity communicated with the waste liquid collecting device, a perfusate cavity communicated with the isolated organ perfusion system, and a semipermeable membrane device communicated between the perfusate cavity and the waste liquid cavity.

In one embodiment, the fluid infusion subsystem comprises a fluid storage part, an infusion pump which is connected between a fluid inlet of the isolated organ perfusion system and the fluid storage part in a penetrating manner, and a pinch valve which is arranged between the fluid storage part and the infusion pump;

the infusion pump is in communication with the control subsystem.

In one embodiment, the fluid infusion subsystem further comprises a third fluid level sensor arranged on the fluid storage part;

the third level sensor is in communication with the control subsystem.

In one embodiment, the system further comprises an alarm device connected with the control subsystem.

In one embodiment, the system further comprises a server which is in communication connection with the control subsystem, and mobile monitoring equipment which is in communication connection with the control subsystem and the server respectively.

One of the above technical solutions has the following advantages and beneficial effects:

the filter subsystem is connected between the isolated organ perfusion system and the liquid storage device in a penetrating way; the control subsystem is respectively in communication connection with the filtering subsystem and the fluid infusion subsystem; the control subsystem can drive the filtering subsystem to enable the perfusate to be filtered of the isolated organ perfusion system to flow to the filtering subsystem, and the filtering subsystem can filter the received perfusate to be filtered and transmit the filtered perfusate back to the isolated organ perfusion system; the control subsystem can also acquire liquid level data of perfusate to be filtered in the liquid storage device and obtain supplement data of the supplement liquid according to the liquid level data; and driving the fluid infusion subsystem to deliver the supplementary fluid to the isolated organ perfusion system according to the supplementary data. The isolated organ perfusion filtration system of each embodiment of this application has realized filtering the micromolecule metabolite that isolated organ produced among the isolated organ perfusion process, harmful metabolite in the perfusate has in time been filtered effectively, and supplement the required supplementary liquid of isolated organ perfusion automatically, it is balanced to keep entire system's perfusate, prevent that harmful metabolite from causing irreversible damage to isolated organ, the perfusion effect of isolated organ has been improved, further save marginal donor organ, improve the transplantable rate of organ.

Drawings

FIG. 1 is a schematic diagram of a first configuration of an ex vivo organ perfusion filtration system in one embodiment;

FIG. 2 is a schematic diagram of a second configuration of an ex vivo organ perfusion filtration system in accordance with an embodiment;

FIG. 3 is a schematic diagram of a third configuration of an ex vivo organ perfusion filtration system in one embodiment;

FIG. 4 is a fourth schematic diagram of an ex vivo organ perfusion filtration system according to one embodiment;

FIG. 5 is a schematic diagram of a fifth configuration of an ex vivo organ perfusion filtration system in accordance with an embodiment;

FIG. 6 is a sixth schematic diagram of an ex vivo organ perfusion filtration system in accordance with an embodiment;

FIG. 7 is a seventh configuration of an ex vivo organ perfusion filtration system in accordance with an embodiment.

Detailed Description

To facilitate an understanding of the present application, the present application will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present application are shown in the drawings. This application may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

In one embodiment, as shown in fig. 1, there is provided an ex vivo organ perfusion filtration system comprising:

the input end of the liquid storage device 110 is used for being communicated with a liquid outlet of the isolated organ perfusion system; the reservoir 110 stores perfusate to be filtered of the isolated organ perfusion system;

the input end of the filtering subsystem 120 is communicated with the output end of the liquid storage device 110, and the output end of the filtering subsystem 120 is communicated with the liquid inlet of the isolated organ perfusion system; the filtration subsystem 120 filters the perfusate to be filtered;

the fluid infusion subsystem 130 is used for communicating with a fluid inlet of the isolated organ perfusion system; the fluid infusion subsystem 130 transmits the fluid infusion to the isolated organ perfusion system;

the control subsystem 140, the control subsystem 140 is connected with the filtration subsystem 120, the fluid infusion subsystem 130 in communication;

wherein, the control subsystem 140 is used for driving the filtration subsystem 120; the control subsystem 140 is further configured to process the acquired liquid level data of the perfusate to be filtered in the reservoir 110, obtain supplement data of the supplement liquid, and drive the fluid replacement subsystem 130 to transmit the supplement liquid to the isolated organ perfusion system based on the supplement data.

In particular, an ex vivo organ perfusion system refers to a preservation system that perfuses an ex vivo organ. The reservoir 110 refers to a container storing perfusate to be filtered; for example, the reservoir 110 may be a liquid storage tank. The filtration subsystem 120 may be used to filter the perfusate to be filtered and to provide power for the transfer of the filtered perfusate. The in-vitro organ perfusion system is connected between the in-vitro organ perfusion system and the liquid storage device 110 in a penetrating manner through the filtering subsystem 120, the in-vitro organ perfusion system can transmit perfusate to be filtered to the liquid storage device 110, the filtering subsystem 120 is driven through the controller subsystem 140, the perfusate to be filtered can be pumped into the filtering subsystem 120, the perfusate to be filtered can be filtered through the filtering subsystem 120, and the filtered perfusate can be transmitted back to the in-vitro organ perfusion system. For example, filtration subsystem 120 may be used to filter out unwanted small molecule metabolites (e.g., water, lactic acid, and inorganic salts, etc.) from the perfusate. It should be noted that the perfusate to be filtered refers to the perfusate output from the liquid outlet of the isolated organ perfusion system.

Based on the control subsystem 140 being communicatively connected to the filtration subsystem 120, the control subsystem 140 may drive the filtration subsystem 120 such that the perfusate to be filtered in the reservoir 110 flows to the filtration subsystem 120 and transfers the filtered perfusate back to the isolated organ perfusion system. Based on the control subsystem 140 being in communication connection with the fluid infusion subsystem 130, the control subsystem 140 can collect the fluid level data of the perfusate to be filtered in the reservoir 110, and obtain the supplement data of the supplement fluid according to the fluid level data. The control subsystem 140 may then drive the fluid infusion subsystem 130 such that the fluid infusion subsystem 130 delivers a corresponding amount of the fluid infusion to the isolated organ perfusion system, thereby achieving perfusion fluid balance of the isolated organ perfusion system.

In one example, the control subsystem collects liquid level data of the perfusate to be filtered in the liquid reservoir, and compares the collected liquid level data with the target liquid level data to obtain the outflow amount of the perfusate to be filtered in the liquid reservoir within a preset time. The control subsystem can obtain the supplement amount of the supplement liquid according to the outflow amount of the perfusate to be filtered, and then the control subsystem can drive the fluid infusion subsystem to ensure that the fluid infusion subsystem transmits the supplement liquid with the supplement amount to the isolated organ perfusion system, so that the perfusate of the isolated organ perfusion system is balanced, and further, the error caused by the environment and the like in the supplement process is reduced; wherein the make-up liquid is supplemented in an amount equal to the amount of the filtered waste liquid and the amount of the environmental error.

It should be noted that, when the system is started, the control subsystem may record an initial liquid level in the liquid reservoir as a baseline, and the initial liquid level is the target liquid level data.

In one example, the filtration subsystem can receive the perfusate to be filtered in the liquid storage device, continuously filter the micromolecule metabolites in the perfusate to be filtered, and automatically and continuously supplement the supplementary liquid required by the perfusion of the new isolated organ through the liquid supplement subsystem, so as to keep the perfusate balance of the whole system; the isolated organ preservation method has the advantages that the isolated organ can be preserved for a long time under the normal temperature environment, organ failure and damage caused by accumulation of harmful metabolites are prevented, thrombi, inflammatory factors and other harmful substances in the isolated organ are effectively removed, the preservation state of the isolated organ is improved, the physiological state of the isolated organ is maintained or improved, the problems of pulmonary edema, renal toxicity, abnormal liver function, poor cardio-pulmonary oxygenation and the like are improved, isolated edge donor organs are effectively preserved, and the utilization rate of the isolated organ is improved.

The isolated organ may be an isolated lung, an isolated kidney, an isolated heart, an isolated pancreas, or the like. The perfusate refers to a nutrient solution which can be used for preserving isolated organs; for example, the perfusate may be Steen solution, LPD solution, Perfadex solution, UW solution, cell preservation solution, blood, etc.

In the isolated organ perfusion and filtration system, the control subsystem can drive the filtration subsystem to enable the perfusate to be filtered of the isolated organ perfusion system to flow to the filtration subsystem, so that the filtration subsystem can filter the received perfusate to be filtered and transmit the filtered perfusate back to the isolated organ perfusion system; the control subsystem can also acquire liquid level data of perfusate to be filtered in the liquid storage device and obtain supplement data of the supplement liquid according to the liquid level data; and driving the fluid infusion subsystem to deliver the supplementary fluid to the isolated organ perfusion system according to the supplementary data. Realize filtering the micromolecule metabolite in waiting to filter the perfusate, and supplement the required replenisher of isolated organ perfusion automatically, it is balanced to keep entire system's perfusate, and then the operation process of perfusate replenisher has been simplified, isolated organ edema in pipeline transmission process of perfusate has been reduced, the error that reservoir evaporation etc. caused, prevent that harmful metabolite from causing irreversible damage to isolated organ, the perfusion effect of isolated organ has been improved, further save marginal donor organ, the transplantable rate of organ is improved.

In one embodiment, as shown in fig. 2, an isolated organ perfusion filtration system is provided, comprising a reservoir 210, a filtration subsystem 220, a fluid replacement subsystem 230, and a control subsystem 240; the control subsystem 240 includes a main control device 242 and a first liquid level sensor 244 disposed on the liquid reservoir 210; the first level sensor 244 is communicatively coupled to the master control device 242.

Wherein the first level sensor 244 may be an ultrasonic level sensor; the first level sensor 244 may be used to measure level data (e.g., level) of the perfusate to be filtered stored in the reservoir 210.

Specifically, a first liquid level sensor 244 may be disposed in the reservoir 210, and the master control device 242 may periodically collect liquid level data measured by the first liquid level sensor 244 based on the first liquid level sensor 244 being communicatively connected to the master control device 242. For example, the master control device 242 may collect the height of the initial fluid level in the reservoir 210 as target fluid level data at system start-up; in the system operation in-process, the periodic collection liquid level data, and compare the processing to the liquid level data who gathers with target liquid level data, when the liquid level descends to predetermineeing when high (or when predetermineeing the time), drive fluid infusion subsystem 230 transmits the make-up fluid to isolated organ perfusion system, liquid level height until reservoir 210 reaches target liquid level data, the realization keeps entire system's perfusate balanced, the isolated organ edema of perfusate in pipeline transmission process has been reduced, the error that causes such as reservoir evaporation, prevent that harmful metabolite from causing irreversible damage to isolated organ, the perfusion effect of isolated organ has been improved, further save the marginal organ, improve the transplantable rate of organ.

In one example, the master device may include an upper computer apparatus, and a lower computer apparatus connected to the upper computer apparatus. The lower computer device is respectively in communication connection with the pipeline filtering subsystem and the fluid infusion subsystem.

The lower computer device may have a processing device of an ARM (Advanced RISC Machines, RISC microprocessor) processor, and the upper computer device may be, but is not limited to, a computer, a notebook computer, a smart phone, a tablet computer, a portable wearable device, and the like.

Specifically, based on the communication connection between the upper computer device and the lower computer device, the upper computer device can transmit the collected operation data of each subsystem to the lower computer device, and meanwhile, the received data can be displayed by the external display device. The lower computer device is respectively in communication connection with the filtering subsystem and the fluid infusion subsystem, can be used for transmitting (collecting, receiving or issuing and the like) operation parameters among the subsystems and is in communication with the upper computer device, so that the operation state of the whole system is monitored in real time.

In one embodiment, as shown in fig. 3, an ex vivo organ perfusion filtration system is provided, comprising a reservoir 310, a filtration subsystem 320, a fluid replacement subsystem 330, and a control subsystem 340; also included is waste liquid collection apparatus 350; the waste fluid collection device 350 is in communication with the waste fluid discharge end of the filtration subsystem 320.

Specifically, the waste collection device 350 may be used to collect material (i.e., waste) filtered by the filtration subsystem. Based on waste liquid collection equipment through connection filters the waste liquid discharge end of subsystem, waste liquid collection equipment can collect the processing with the material that filters the subsystem and filter.

In one embodiment, as shown in FIG. 3, waste collection apparatus 350 includes a waste collection device 352 connected through the discharge end of the filtration subsystem, and a second level sensor 354 disposed on waste collection device 352; the second fluid level sensor 354 is communicatively coupled to the control subsystem 340.

Wherein waste collection device 352 is configured to collect waste; waste collection 352 may be a waste tank. The second liquid level sensor 354 may be an ultrasonic liquid level sensor; second level sensor 354 may be used to measure level data (e.g., liquid level) of waste collected in waste collection device 352.

Specifically, based on second liquid level sensor 354 being communicatively connected to control subsystem 340, second liquid level sensor 340 may measure a waste liquid collecting condition of waste liquid collecting device 352 in real time, and control subsystem 340 may collect a remaining capacity of waste liquid measured by second liquid level sensor 354, and then control subsystem 340 may monitor a remaining capacity condition of waste liquid in waste liquid collecting device 352 in real time. For example, when monitoring that waste liquid collection device 352 is overfilled, control subsystem 340 generates an early warning to prompt the user to timely dispose of the waste liquid collected in waste liquid collection device 352, thereby preventing overfilling of waste liquid in waste liquid collection device 352 and resulting in overflow of the waste liquid.

In one embodiment, as shown in fig. 4, an ex vivo organ perfusion filtration system is provided, comprising a reservoir 410, a filtration subsystem 420, a fluid replacement subsystem 430, and a control subsystem 440; wherein filtration subsystem 420 includes a first tubing assembly 422, a second tubing assembly 424, a filtration device 426, and a power pump 428 communicatively coupled to the control subsystem. A power pump 428 is connected through the first conduit assembly 422 between the reservoir 410 and the filter apparatus 426; the filter device 426 is in communication with the isolated organ perfusion system via a second tubing assembly 424.

In particular, the filtering device 426 can filter the perfusate to be filtered, so as to effectively filter the metabolites generated by the isolated organ, such as excessive water, lactic acid, inorganic salts, harmful renal toxicants and the like. The first pipe assembly 422 can be used for conveying perfusate to be filtered output by the reservoir 410; first tubing assembly 422 includes, but is not limited to, tubing, two-way joints, three-way joints, micro-plug filters, leukocyte-reduction devices, and bridge tubing. The second tubing assembly 424 may be used to deliver filtered perfusate to an ex vivo organ perfusion system; the second tubing assembly 424 includes, but is not limited to, tubing, two-way joints, three-way joints, micro-plug filters, leukocyte reduction devices, and bridge tubing. The first tubing assembly 422 and the second tubing assembly 424 form a transfer path for the perfusion fluid. The power pump 428 may be used to provide transmission power to the first and second pipeline assemblies 422 and 424, so as to continuously output the perfusate to be filtered to the isolated organ perfusion system, and automatically and continuously transmit the filtered perfusate and the supplementary fluid required for supplementing new isolated organ perfusion back to the isolated organ perfusion system, so as to keep the perfusate balance of the isolated organ perfusion system. Alternatively, the power pump 428 is a centrifugal pump, a roller pump, or a peristaltic pump.

In one particular embodiment, as shown in fig. 4, filtration subsystem 420 further includes a pressure sensor 432 disposed in first conduit assembly 422; pressure sensor 432 is communicatively coupled to a control subsystem 440.

Wherein the pressure sensor 432 may be used to measure the pressure of the perfusate to be filtered during the flow of the first tubing assembly 422. Subsystem 440 is controlled based on pressure sensor 432 communication link. The control subsystem 440 can collect the pipeline pressure measured by the pressure sensor 432, and can control the rotating speed of the power pump in a linkage manner according to the pipeline pressure, so that the outflow speed of the waste liquid in the filtering equipment can be dynamically controlled.

In a specific embodiment, as shown in fig. 4, the filtering device comprises a waste liquid cavity communicated with the waste liquid collecting device, a perfusate cavity communicated with the isolated organ perfusion system, and a semipermeable membrane device communicated between the perfusate cavity and the waste liquid cavity.

Wherein, the semipermeable membrane device can be used for filtering metabolites in the perfusate; the waste liquid cavity can be used for containing metabolites filtered by the semipermeable membrane device; the perfusate chamber can be used to contain the perfusate to be filtered.

Specifically, through perfusate cavity through connection between separation organ perfusion system and pellicle device, can carry out filtration treatment in the to-be-filtered perfusate flow direction pellicle device with the transmission of separation organ perfusion system to and can flow back separation organ perfusion system with the perfusate after filtering in the pellicle device. Through waste liquid cavity through-connection between pellicle device and waste liquid collecting equipment, the pellicle device can pass through waste liquid cavity flow direction waste liquid collecting equipment with the waste liquid that the filtration obtained. The semi-permeable membrane device is connected between the perfusate cavity and the waste liquid cavity in a penetrating manner, so that harmful metabolites in the isolated organ perfusate can be effectively removed, the safety of the perfusate is ensured, the using amount of the perfusate is greatly saved, the liquid changing process is simple and safe to operate, and the manual liquid changing error is reduced.

In one example, the semi-permeable membrane device comprises a functional semi-permeable membrane, and different semi-permeable membranes can be selected according to different types of isolated organs, so that different metabolites can be filtered. The perfusate to be filtered flows through the perfusate cavity from the isolated organ perfusion system, is filtered by the semipermeable membrane device, can be mixed with the added supplementary liquid, and is pumped into the isolated organ perfusion system again; the filtered waste liquid is discharged to the waste liquid collecting device through the waste liquid cavity.

In one example, the perfusate cavity may comprise a first cavity and a second cavity, the first cavity is connected between the isolated organ perfusion system and the semipermeable membrane device; the first cavity is communicated and connected between the isolated organ perfusion system and the semipermeable membrane device; the first cavity and the second cavity are isolated from each other. The perfusate to be filtered transmitted by the body organ perfusion system can flow to the semipermeable membrane device through the first cavity; the filtered perfusate transmitted by the semipermeable membrane device can flow to the isolated organ perfusion system through the second cavity.

In one embodiment, as shown in fig. 5, an ex vivo organ perfusion filtration system is provided, comprising a reservoir 510, a filtration subsystem 520, a fluid replacement subsystem 530, and a control subsystem 540; the fluid infusion subsystem 530 comprises a fluid storage part 532, an infusion pump 534 which is connected between a fluid inlet of the isolated organ perfusion system and the fluid storage part 532 in a penetrating manner, and a pinch valve 536 which is arranged between the fluid storage part 532 and the infusion pump 534; infusion pump 534 is communicatively coupled to control subsystem 540.

Wherein the reservoir 532 may be used to store a refill fluid. The fluid reservoir 532 may be, but is not limited to, a fluid reservoir (e.g., a graduated fluid reservoir) or a fluid reservoir bag (e.g., a 3L medical bag). The infusion pump 534 may be used to power the delivery of the refill fluid. The infusion pump 534 may be an intelligent infusion pump with flow control and bubble detection functions; when the intelligent infusion pump detects abnormal flow or detects bubbles in the replenishing liquid, an alarm is triggered to remind. Pinch valve 536 refers to a pipeline switch, and pinch valve 536 can be used to control the on/off of a pipeline; pinch valve 536 may be an automatic pinch valve controlled by a control subsystem or a manually controlled valve switch.

Specifically, the liquid storage member 532, the infusion pump 534, the pinch valve 536 and the isolated organ perfusion system can be in through connection through a pipeline, and the supplementary liquid in the liquid storage member 532 can be pumped into the isolated organ perfusion system through the power provided by the infusion pump 534, so that the supplementary liquid required by the isolated organ perfusion can be automatically supplemented, the perfusate balance of the whole system is kept, the operating process of perfusate supplementation is simplified, and the fluid supplementation efficiency is improved.

Further, when the flow rate of the replenishing liquid is detected to be abnormal or the replenishing liquid contains air bubbles, the controllable liquid stopping valve is closed, and early warning is generated.

In one example, the fluid replacement subsystem further includes a flow sensor disposed between the infusion pump and the fluid reservoir. The flow sensor is in communication with the control subsystem.

The flow sensor can be used for measuring the flow rate of the supplementing liquid in the measuring pipeline and the bubble content of the supplementing liquid. Optionally, the flow sensor is an ultrasonic flow sensor, an optical flow sensor or an electrical flow sensor. The flow sensor may be a flow sensor integrated in the infusion pump or may be a flow sensor independent from the infusion pump.

Specifically, the control subsystem may collect a replenishment flow of the replenishment solution measured by the flow sensor based on the flow sensor communication link. The control subsystem can monitor the supplement flow and the bubble content of the supplement liquid output by the liquid storage part in real time; for example, the control subsystem can achieve error early warning on the actually acquired supplement flow, adjust the flow of the supplement liquid in time and improve the accuracy of perfusion of the supplement liquid; the control subsystem can also trigger alarm reminding when the actually acquired bubble content reaches an early warning value.

It should be noted that, depending on the type of the flow sensor, the connection mode of the flow sensor is different, and a non-contact connection is common.

In the isolated organ perfusion filtration system, the infusion pump provides power to pump the supplementary liquid into the filtration subsystem, the filtration subsystem filters the perfusate to be filtered to obtain filtered perfusate, and the filtered perfusate and the supplementary liquid provided by the supplementary liquid subsystem are pumped and returned to the isolated organ perfusion system, so that the perfusate of the isolated organ perfusion system is always in a balanced state.

In one embodiment, as shown in FIG. 5, the fluid infusion subsystem 530 further includes a third fluid level sensor 538 disposed in the fluid reservoir 532; the third level sensor 538 is communicatively coupled to the control subsystem 540.

Wherein the third level sensor 538 may be an ultrasonic level sensor; the third level sensor 538 may be used to measure level data (e.g., a fluid level) of the refill fluid stored in the reservoir 532.

Specifically, based on the third level sensor 538 being communicatively coupled to the control subsystem 510, the control subsystem 510 may collect the remaining volume of the replenishment liquid measured by the third level sensor 538, and the control subsystem 510 may monitor the remaining volume of the replenishment liquid in the reservoir 532 in real time. For example, when the third level sensor 538 monitors that the fluid level in the reservoir 432 is below a predetermined threshold, an alarm alert is triggered to prevent insufficient replenishment fluid from the reservoir 532, which may cause the perfusion fluid of the system to lose balance.

The supplementary liquid contains nutrients required for normal metabolism of the isolated organ, such as antibiotics, vitamins, drugs, and buffers for adjusting the pH of the perfusate.

In one embodiment, as shown in fig. 6, an ex vivo organ perfusion filtration system is provided, comprising a reservoir 610, a filtration subsystem 620, a fluid replacement subsystem 630, and a control subsystem 640; an alarm 650 is also included and is connected to the control subsystem 640.

The alarm 650 may be a buzzer or a flashing light, or a combination of the buzzer and the flashing light.

Specifically, based on the connection between the alarm device 650 and the control subsystem 640, when the control subsystem 640 monitors an abnormality (such as, but not limited to, an abnormal flow rate of the replenishment liquid, an abnormal residual volume of the replenishment liquid, a bubble in the pipeline, an abnormal system or abnormal storage), the alarm device 650 is triggered, so that the alarm device 650 sends out an alarm message.

In one embodiment, an ex vivo organ perfusion filtration system is provided, comprising a reservoir 710, a filtration subsystem 720, a fluid replacement subsystem 730, and a control subsystem 740; also included is a server 750 communicatively coupled to the control subsystem 740, and a mobile monitoring device 760 communicatively coupled to the control subsystem 740 and the server 750, respectively.

Wherein, the server 750 can be used to store data generated by the system (such as the liquid level data of the perfusion fluid to be filtered and the supplement data of the supplement fluid); the server 750 may store data and share data through the cloud; the server 750 may be implemented as a stand-alone server or a server cluster composed of a plurality of servers. The mobile monitoring device 760 may be, but is not limited to, a cell phone, a tablet or a computer, etc. The mobile monitoring device 760 may be used to transmit relevant data, and may also be used to display data and remotely operate various subsystems.

The filtering subsystem 720 is connected between the isolated organ perfusion system and the reservoir 710 in a penetrating way; the control subsystem 740 is respectively in communication connection with the filtering subsystem 720 and the fluid infusion subsystem 730; the control subsystem 740 may drive the filtration subsystem 720 such that the perfusate to be filtered of the isolated organ perfusion system flows to the filtration subsystem 720, and the filtration subsystem 720 may filter the received perfusate to be filtered and transmit the filtered perfusate back to the isolated organ perfusion system; the control subsystem 740 may also collect liquid level data of the perfusate to be filtered in the reservoir 710, and obtain supplement data of the supplementary liquid according to the liquid level data; and according to the supplementary data, drive fluid replacement subsystem forms the circulation fluid replacement to isolated organ perfusion system transmission supplementary solution, realizes keeping entire system's perfusate balanced, prevents that harmful metabolite from causing irreversible damage to isolated organ, has improved the perfusion effect of isolated organ, further saves marginal donor organ, improves the transplantable rate of organ. The control subsystem 740 is in communication with the filtration subsystem 720 and the fluid replacement subsystem 730, respectively, and the control subsystem 740 receives and analyzes the operational parameter data from the filtration subsystem 720 and the fluid replacement subsystem 730, and may issue control commands to the filtration subsystem 720 and the fluid replacement subsystem 730 to adjust the operational parameters thereof. Meanwhile, the control subsystem 740 may also upload the real-time monitored data to the server 750 for storage. The control subsystem 740 can read the relevant operating parameters in the server 750 or receive the instructions sent by the mobile monitoring device 760, control and adjust the operating parameters of the system, so as to realize remote real-time monitoring of the filtering operation of the perfusate and simplify the operation process of filtering the perfusate.

In one example, the communication connection among the control subsystem, the server and the mobile monitoring device may be a cable connection, a wireless connection or an internet of things connection; for example, the communication connection mode may be WIFI communication, mobile communication, optical fiber communication, or the like.

It should be noted that the relevant data of perfusion fluid replacement for different isolated organs may share the same server, or may be stored in different servers.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.