阅读说明：本技术 一种小分子鞣花酸溶液的制备方法 (Preparation method of micromolecular ellagic acid solution ) 是由 敖德平 于 2019-10-25 设计创作，主要内容包括：本发明属于化妆品原料技术领域,具体公开了一种小分子鞣花酸溶液的制备方法。该方法包括以下步骤：发酵菌悬液制备、石榴皮的固态发酵、萃取和鞣花酸溶液制备,本发明的鞣花酸经过了特殊工艺处理,改变了鞣花酸吸收不好,不稳定的特点,使溶液中的鞣花酸变成小分子,容易吸收,效果见效快,且性质稳定,改进的小分子鞣花酸美白效果是普通鞣花酸五倍,将其应用于制备具有抗氧化、美白、抗衰老功效的护肤品具有显著的应用的前景。(The invention belongs to the technical field of cosmetic raw materials, and particularly discloses a preparation method of a micromolecule ellagic acid solution. The method comprises the following steps: the ellagic acid is treated by a special process, so that the characteristics of poor absorption and instability of the ellagic acid are changed, the ellagic acid in the solution is changed into small molecules which are easy to absorb, quick in effect and stable in property, the improved small-molecule ellagic acid whitening effect is five times that of the common ellagic acid, and the application of the improved small-molecule ellagic acid whitening effect in preparing skin care products with the effects of resisting oxidation, whitening and resisting aging has a remarkable application prospect.)

1. A preparation method of a small molecule ellagic acid solution is characterized by comprising the following steps:

(1) hair-like devicePreparing a yeast suspension: inoculating Aspergillus niger spore and Candida yeast suspension into nutrient medium, stirring, and mixing to obtain zymocyte suspension containing Aspergillus niger spore number not less than 1 × 10⁸Per mL, Candida greater than or equal to 1 × 10⁷The nutrient medium comprises the following components in percentage by volume: NaNO₃ 2～4g/L、K₂HPO₄ 1～2g/L、MgSO₄0.5-1 g/L, KCl 0.5.5-1 g/L, 25-35 g/L glucose, and water as solvent;

(2) solid-state fermentation of pomegranate rind: drying and crushing fresh pomegranate rind to obtain pomegranate rind powder; adding a fermentation bacteria suspension into the pomegranate peel powder, wherein the using amount of the fermentation bacteria suspension accounts for 35-50% of the weight of the pomegranate peel powder, stirring and uniformly mixing to obtain a fermentation material, placing the fermentation material into a fermentation tank, fermenting for 5-6 days at the temperature of 28-35 ℃ and the relative humidity of 85-90%, stopping fermentation to obtain a fermentation product, and drying the fermentation product for later use;

(3) and (3) extraction: crushing the dried fermentation product again, adding anhydrous methanol which is 8-12 times of the total weight of the fermentation product, soaking for 30-60 minutes, extracting under reflux for 2-3 times, filtering for 1-3 hours each time, combining filtrates, performing rotary evaporation on the filtrate to recover methanol, washing the obtained solid with deionized water, and freeze-drying to obtain ellagic acid freeze-dried powder;

(4) preparation of ellagic acid solution: weighing 1.03g of ellagic acid freeze-dried powder, adding 20mL of deionized water, introducing nitrogen, carrying out the whole reaction process under the nitrogen atmosphere, placing a reaction container containing an ellagic acid aqueous solution in a water bath at 15-30 ℃ for reaction for 3-5 h, carrying out shading treatment on the reaction container, and ensuring that the reaction is carried out under the condition of keeping out of the sun, thereby obtaining the micromolecular ellagic acid solution.

2. The method for preparing a small molecule ellagic acid solution according to claim 1, wherein the inoculum size of the suspension of aspergillus niger spores and candida albicans is 1% v/v.

3. The method for preparing a small molecule ellagic acid solution of claim 1, wherein the nutrient medium consists of: n is a radical ofaNO₃ 3g/L、K₂HPO₄ 1.5g/L、MgSO₄0.5g/L, KCl 0.5.5 g/L, glucose 35g/L, and water as solvent.

4. The method for preparing the small molecule ellagic acid solution according to claim 1, wherein a reaction vessel containing the ellagic acid aqueous solution is placed in a water bath at 25 ℃ for reaction for 4 hours.

5. A skin care product comprising 10 to 30 wt.% of the small molecule ellagic acid solution produced by the method for producing a small molecule ellagic acid solution according to any one of claims 1 to 4.

6. The skin care product of claim 5, wherein the skin care product is a facial mask, essence, cream or lotion with antioxidant, whitening and/or anti-aging effects.

Technical Field

The invention belongs to the technical field of cosmetic raw materials, and particularly relates to a preparation method of a small-molecule ellagic acid solution.

Background

Ellagic acid is a polyphenol dilactone, a dimeric derivative of gallic acid. Ellagic acid is a natural polyphenol component widely existing in plant tissues such as soft fruit and nut, and has blue color development reaction with ferric trichloride, yellow color when meeting sulfuric acid, and positive Greiss-meger reaction, and is also easy to react with metal cation such as Ca²⁺、Mg²⁺And (4) combining. Through the laboratory research of HSI (health Sciences institute) and the recent laboratory research of south Korean University (Ca tholic University of south Korea), the C element in the ellagic acid is found to increase the efficacy of the ellagic acid under the condition of adding the catalyst at high temperature, and the C element in the ellagic acid does not change the ellagic acidThe original molecular structure. Ellagic acid prepared by the prior art has large molecular weight, is not easy to be absorbed by skin, has unstable property, and limits the use of the ellagic acid as a cosmetic raw material.

Disclosure of Invention

The invention aims to provide a preparation method of a small-molecule ellagic acid solution. The ellagic acid is treated by a special process, so that the characteristics of poor absorption and instability of the ellagic acid are changed, the ellagic acid in the solution is changed into small molecules, the absorption is easy, the effect is quick, and the improved small-molecule ellagic acid whitening effect is five times that of the common ellagic acid.

The invention is realized by the following technical scheme:

a preparation method of a small molecule ellagic acid solution comprises the following steps:

(1) preparing a zymocyte suspension: inoculating Aspergillus niger spore and Candida yeast suspension into nutrient medium, stirring, and mixing to obtain zymocyte suspension containing Aspergillus niger spore number not less than 1 × 10⁸Per mL, Candida greater than or equal to 1 × 10⁷The nutrient medium comprises the following components in percentage by volume: NaNO₃ 2～4g/L、K₂HPO₄ 1～2g/L、MgSO₄0.5-1 g/L, KCl 0.5.5-1 g/L, 25-35 g/L glucose, and water as solvent;

(2) solid-state fermentation of pomegranate rind: drying and crushing fresh pomegranate rind to obtain pomegranate rind powder; adding a fermentation bacteria suspension into the pomegranate peel powder, wherein the using amount of the fermentation bacteria suspension accounts for 35-50% of the weight of the pomegranate peel powder, stirring and uniformly mixing to obtain a fermentation material, placing the fermentation material into a fermentation tank, fermenting for 5-6 days at the temperature of 28-35 ℃ and the relative humidity of 85-90%, stopping fermentation to obtain a fermentation product, and drying the fermentation product for later use;

(3) and (3) extraction: crushing the dried fermentation product again, adding anhydrous methanol which is 8-12 times of the total weight of the fermentation product, soaking for 30-60 minutes, extracting under reflux for 2-3 times, filtering for 1-3 hours each time, combining filtrates, performing rotary evaporation on the filtrate to recover methanol, washing the obtained solid with deionized water, and freeze-drying to obtain ellagic acid freeze-dried powder;

(4) preparation of ellagic acid solution: weighing 1.03g of ellagic acid freeze-dried powder, adding 20mL of deionized water, introducing nitrogen, carrying out the whole reaction process under the nitrogen atmosphere, placing a reaction container containing an ellagic acid aqueous solution in a water bath at 15-30 ℃ for reaction for 3-5 h, carrying out shading treatment on the reaction container, and ensuring that the reaction is carried out under the condition of keeping out of the sun, thereby obtaining the micromolecular ellagic acid solution.

Further, the inoculation amount of the Aspergillus niger spore and Candida yeast suspension is 1% v/v.

Further, the nutrient medium comprises the following components: NaNO₃ 3g/L、K₂HPO₄ 1.5g/L、MgSO₄0.5g/L, KCl 0.5.5 g/L, glucose 35g/L, and water as solvent.

Preferably, in the step (4), the reaction vessel containing the ellagic acid aqueous solution is placed in a water bath at 25 ℃ for reaction for 4 hours.

The invention also provides a skin care product, which contains 10-30 wt.% of the small-molecule ellagic acid solution prepared by the preparation method of the small-molecule ellagic acid solution. Preferably, the skin care product is a facial mask liquid, essence, cream or emulsion with antioxidant, whitening and/or anti-aging effects.

Compared with the prior art, the invention has the following beneficial effects:

the ellagic acid is treated by a special process, so that the characteristics of poor absorption and instability of the ellagic acid are changed, the ellagic acid in the solution is changed into small molecules, the ellagic acid is easy to absorb, the effect is quick to take effect, the property is stable, the improved small-molecule ellagic acid has a whitening effect five times that of the common ellagic acid, and the application of the ellagic acid in preparing skin care products with the effects of resisting oxidation, whitening and resisting aging has a remarkable application prospect.

Detailed Description

The following examples are further illustrative of the present invention and are not intended to be limiting thereof. The components of the formulations in the following examples are, unless otherwise specified, conventional commercial products.