阅读说明：本技术 一种多色荧光阅读仪 (Multicolor fluorescence reader ) 是由 车团结 徐红 李春 沈颂东 张莹 李潇玲 杨涛 于 2019-08-09 设计创作，主要内容包括：本发明涉及荧光检测技术领域,具体涉及一种多色荧光阅读仪,旨在解决现有技术中的不能进行快速准确定量检测的缺陷,从而提供一种多色荧光阅读仪,包括图像光学收集模块,包括具有图像传感器的摄像头和光学元件；数据采集模块,包括信号放大器和模数转换单元,通过图像光学收集模块收集的信号进入信号放大器放大后,再输入模数转换单元进行模数转换；数据处理模块,与数据采集模块连接,包括中央处理器和显示屏,顺次连接的图像光学收集模块、数据采集模块、数据处理模块,将其很好的连接起来充分发挥对高速并行数据的处理优势,能够很好的实现高速并行采集和同步远程传输存储,同样的在保证有较快的操作速度的同时也具有很高的灵活性。(The invention relates to the technical field of fluorescence detection, in particular to a multicolor fluorescence reader, aiming at solving the defect that rapid and accurate quantitative detection cannot be carried out in the prior art, and providing the multicolor fluorescence reader, which comprises an image optical collection module, a camera with an image sensor and an optical element, wherein the image optical collection module comprises a first lens and a second lens; the data acquisition module comprises a signal amplifier and an analog-to-digital conversion unit, and signals collected by the image optical collection module enter the signal amplifier to be amplified and then are input into the analog-to-digital conversion unit to be subjected to analog-to-digital conversion; the data processing module is connected with the data acquisition module and comprises a central processing unit and a display screen, the image optical collection module, the data acquisition module and the data processing module are connected in sequence, the processing advantages of the image optical collection module, the data acquisition module and the data processing module to high-speed parallel data are fully exerted by well connecting the data processing module, high-speed parallel acquisition and synchronous remote transmission storage can be well realized, and the data processing module has high flexibility while ensuring higher operation speed.)

1. A multi-color fluorescence reader, comprising:

an image optical collection module (1) comprising a camera (11) with an image sensor and an optical element (12);

the data acquisition module (2) comprises a signal amplifier (21) and an analog-to-digital conversion unit (22), and signals collected by the image optical collection module (1) enter the signal amplifier (21) for amplification and then are input into the analog-to-digital conversion unit (22) for analog-to-digital conversion;

and the data processing module (3) is connected with the data acquisition module (2) and comprises a central processing unit (31) and a display screen (32).

2. The multicolor fluorescence reading instrument according to claim 1, wherein the optical elements (12) are provided in plurality and placed on both sides of the camera head (11), the optical elements (12) are an excitation light source (121) with strong light and an analyzing portion (122), the analyzing portion (122) comprises a filter (1221), a light equalizing sheet (1222) and a concave lens (1223), and the centers of the optical elements (12) are on the same horizontal line.

3. The multicolor fluorescence reader according to claim 2, wherein the optical filter (1221) filters the light emitted from the excitation light source (121) to define a wavelength range of the light passing through the optical filter (1221), the light uniformizer uniformizes the light passing through the light uniformizer, and the concave lens (1223) diffuses the light passing through the light uniformizer.

4. A multicolor fluorescence reader according to claim 3, wherein the object to be analyzed undergoes fluorescence reaction by illumination and reflects light to the camera (11), a reflector (111) is added in front of the camera (11), and the reflector (111) is arranged at the bottom of the object to be analyzed.

5. A multicolor fluorescence reader according to claim 4, wherein said camera (11) transmits the collected light signals to said data acquisition module (2), said data acquisition module (2) in turn transmitting the signals to said data processing module (3).

6. The multi-color fluorescence reader of claim 5, wherein the signal amplification unit comprises a programmable amplification circuit (211), a differential amplification circuit (212) and a filter circuit (213), and the reflected signal is amplified by the programmable amplification circuit (211) and the differential amplification circuit (212), filtered by the filter circuit (213), and input to the analog-to-digital conversion unit (22) for analog-to-digital conversion.

7. A multicolor fluorescence reader according to claim 6, wherein said data acquisition module (2) further comprises a compensation unit (23) and said data processing module (3) further comprises a memory module.

8. The multicolor fluorescence reader according to claim 7, further comprising a power module (4), wherein said power module (4) comprises a connection port (41) and a voltage conversion unit (42), and wherein said voltage conversion unit (42) adjusts the voltage value output of the power board.

Technical Field

The invention relates to the technical field of fluorescence detection, in particular to a multicolor fluorescence reader.

Background

The flow cytometer is a novel high-tech instrument integrating laser technology, fluid mechanics technology, photoelectric measurement technology, electronic computer technology, cell fluorescence chemical technology and cell immunity technology, and has the functions of analyzing and sorting cells. The single cells or other particles flowing in a high speed and straight line in the suspension are collected and measured by the scattered light and the marked fluorescence generated when passing through the detection region, so that high-speed one-by-one multi-parameter quantitative analysis is realized. Has wide application in the fields of cell biology, cell dynamics, immunology, hematology, oncology and the like.

The suspension biochip technology is also called flow technology, and is a great improvement on the microarray technology. In the prior art, the method is technically characterized in that: microspheres with different colors are used as carriers of different biological probes, the colors of the microspheres correspond to the types of the biological probes one by one, the microspheres are subjected to bicolor fluorescent markers with different intensities, and the microspheres are arranged into a line on a determined track through a liquid flow field during detection and sequentially pass through a detection area. Two beams of laser are irradiated on the detection area in a crossing way, one beam is used for detecting the marking color of the microsphere, and the other beam is used for measuring the fluorescence intensity of the component to be detected.

Due to the particularity of the fluorescence detection light path, the sensitivity of the camera to fluorescence is weak, and the precision of the detection result is easy to be insufficient according to the conventional structural design, although the method for realizing the rapid quantitative detection of multiple analytes simultaneously exists in the prior art, a fluorescence detection system capable of simultaneously performing the rapid and accurate quantitative detection of the multiple analytes does not exist at present.

Disclosure of Invention

Therefore, the technical problem to be solved by the invention is to overcome the defect that the rapid and accurate quantitative detection cannot be carried out in the prior art, thereby providing a multicolor fluorescence reader.

The technical purpose of the invention is realized by the following technical scheme:

a multi-color fluorescent reader comprising:

an image optical collection module including a camera having an image sensor and an optical element;

the data acquisition module comprises a signal amplifier and an analog-to-digital conversion unit, and signals collected by the image optical collection module enter the signal amplifier to be amplified and then are input into the analog-to-digital conversion unit to be subjected to analog-to-digital conversion;

and the data processing module is connected with the data acquisition module and comprises a central processing unit and a display screen.

Furthermore, a plurality of optical elements are arranged on two sides of the camera, the optical elements are respectively an excitation light source with strong light and an analysis portion, the analysis portion comprises an optical filter, a light equalizing sheet and a concave lens, and the centers of the optical elements are on the same horizontal line.

Further, the optical filter filters the light emitted by the excitation light source, the wavelength range of the light passing through the optical filter is limited, the light homogenizing sheet makes the light passing through the light homogenizing sheet uniform, and the concave lens diffuses the light passing through the light homogenizing sheet.

Furthermore, the object to be detected to be analyzed is subjected to fluorescence reaction through illumination, light is reflected to the camera, a reflector is additionally arranged in front of the camera, and the reflector is arranged at the bottom of the object to be detected.

Further, the camera transmits the collected optical signals to the data acquisition module, and the data acquisition module transmits the signals to the data processing module.

Furthermore, the signal amplifying unit comprises a program-controlled amplifying circuit, a differential amplifying circuit and a filter circuit, and the reflected signal is amplified by the program-controlled amplifying circuit and the differential amplifying circuit, then filtered by the filter circuit, and input into the analog-to-digital conversion unit for analog-to-digital conversion.

Furthermore, the data acquisition module further comprises a compensation unit, and the data processing module further comprises a storage unit.

The power supply module comprises a connection port and a voltage conversion unit, and the voltage conversion unit adjusts the voltage value output of the power supply plate.

The technical scheme of the invention has the following advantages:

1. the multicolor fluorescence reader provided by the invention is characterized in that the image optical collection module, the data acquisition module and the data processing module are sequentially connected, the image optical collection module, the data acquisition module and the data processing module are well connected, the processing advantage of high-speed parallel data is fully exerted, high-speed parallel acquisition and synchronous remote transmission and storage can be well realized, and high flexibility is realized while higher operation speed is ensured.

2. According to the multicolor fluorescence reader provided by the invention, the optical element can enhance the aim of accurately acquiring the fluorescence signal by the camera, so that the detection precision is improved, and the arrangement of the optical filter, the light homogenizing sheet and the concave lens, and the centers of the excitation light source, the optical filter, the light homogenizing sheet and the concave lens are on the same horizontal line, so that the aim of improving the detection precision is fulfilled, the floor area of the whole multicolor fluorescence reader is reduced, and the aim of performing quick and accurate quantitative fluorescence detection on various analytes while being light and handy is achieved.

3. The multicolor fluorescence reader provided by the invention utilizes the reflector to enable the camera to detect double fluorescence, thereby improving the sensitivity and the signal-to-noise ratio of fluorescence detection, and simultaneously can package the camera, the reflector, the optical element, the light analysis part, the data acquisition module and the data processing module together, has portability, small volume and low cost, and can be separated from a special laboratory for field detection.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic diagram of a multicolor fluorescence reader provided in one embodiment of the present invention;

FIG. 2 is a schematic view of the internal module connections of a multicolor fluorescence reader provided in one embodiment of the present invention;

FIG. 3 is a flow chart of fluorescence detection for a multicolor fluorescence reader provided in one embodiment of the present invention.

Description of reference numerals:

1. an image optical collection module; 11. a camera; 111. a mirror; 12. an optical element; 121. an excitation light source; 122. a light analyzing section; 1221. an optical filter; 1222. homogenizing a light sheet; 1223. a concave lens; 2. a data acquisition module; 21. a signal amplifier; 211. a program-controlled amplifying circuit; 212. a differential amplifier circuit; 213. a filter circuit; 22. an analog-to-digital conversion unit; 23. a compensation unit; 3. a data processing module; 31. a central processing unit; 32. a display screen; 33. a storage unit; 4. a power supply module; 41. a connection port; 42. a voltage conversion unit.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

A multicolor fluorescence reading instrument is disclosed, as shown in figure 1, an image optical collection module 1 comprises a camera 11 with an image sensor and an optical element 12, the optical element 12 is provided with a plurality of excitation light sources 121 with strong light and an analysis portion 122 with strong light, the analysis portion 122 comprises an optical filter 1221, a light homogenizing sheet 1222 and a concave lens 1223, the centers of the optical elements 12 are on the same horizontal line, the optical filter 1221 filters the light emitted by the excitation light sources 121, the wavelength range of the light passing through the optical filter 1221 is limited to 365 +/-10 nm, the light passing through the light homogenizing sheet is homogenized, the concave lens 1223 diffuses the light passing through the light homogenizing sheet, the optical element 12 has the purpose of enhancing the accurate collection of fluorescence signals by the camera 11, thereby improving the detection precision, and the arrangement of the optical filter 1221, the light homogenizing sheet and the concave lens 1223, and the excitation light source 121, the optical filter 1221, the light homogenizing sheet and the concave lens 1223 are positioned on the same horizontal line, so that the detection precision is improved, the occupied area of the whole multicolor fluorescence reader is reduced, and the aim of performing rapid and accurate quantitative fluorescence detection on multiple analytes while being light and handy can be achieved.

As shown in fig. 1, the object to be analyzed undergoes a fluorescence reaction by light irradiation, and reflects the light to the camera 11, the reflector 111 is added in front of the camera 11, the reflector 111 is arranged at the bottom of the object to be analyzed, and the reflector 111 is utilized to enable the camera 11 to detect double fluorescence, so that the sensitivity and the signal-to-noise ratio of fluorescence detection are improved, and meanwhile, the camera 11, the reflector 111, the optical element 12, the light analyzing part 122, the data acquisition module 2 and the data processing module 3 can be packaged together, so that the portable detection device has portability, small volume and low cost, and can be separated from a special laboratory for field detection.

As shown in fig. 2, the camera 11 transmits the collected optical signal to the data acquisition module 2, the data acquisition module 2 transmits the signal to the data processing module 3, the data acquisition module 2 comprises a signal amplifier 21 and an analog-to-digital conversion unit 22, the data processing module 3 is connected with the data acquisition module 2, the data acquisition module comprises a central processing unit 31 and a display screen 32, the image optical acquisition module 1, the data acquisition module 2 and the data processing module 3 which are connected in sequence are connected well, the processing advantages of high-speed parallel data are fully exerted, high-speed parallel acquisition and synchronous remote transmission and storage can be well realized, and the same high flexibility is realized while the higher operation speed is ensured.

As shown in fig. 2, the signal amplifying unit includes a program-controlled amplifying circuit 211, a differential amplifying circuit 212, and a filter circuit 213, the reflected signal is amplified by the program-controlled amplifying circuit 211 and the differential amplifying circuit 212, then filtered by the filter circuit 213, and input to the analog-to-digital conversion unit 22 for analog-to-digital conversion, and the data acquisition module 2 further includes a compensation unit 23, thereby avoiding the influence of the micro-pulse caused by the debris and the like on the data acquisition.

As shown in fig. 2, the data processing module 3 further includes a storage unit 33 and a power module 4, the storage unit 33 can prepare for collecting a large amount of data, store all collected original signals, and can ensure the integrity of information, and when data analysis is performed in a later period, the result is more accurate, the power module 4 includes a connection port 41 and a voltage conversion unit 42, and the voltage conversion unit 42 adjusts the voltage value of the power board block to output.

As shown in fig. 3, it is a flowchart of the collection and analysis of the whole working process, and the signal is amplified after being acquired, then analog-to-digital conversion is performed, and signal compensation is performed simultaneously to reduce the influence of optical noise and electrical noise on the pulse electrical signal, further improve the signal-to-noise ratio of the pulse electrical signal, and then data analysis processing is performed, and finally quantitative batch output is performed.

The working principle of the multicolor fluorescence reader is as follows: an analyte to be detected is placed above the reflecting mirror 111 below the camera 11, is irradiated by the excitation light source 121, passes through the light analyzing part 122, is reflected into the camera 11 for signal collection, is amplified, is subjected to analog-to-digital conversion and signal compensation, is subjected to data analysis processing, and is output in a quantitative batch.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.