阅读说明：本技术 小蓟炭量子点（纳米颗粒/纳米粒子）的制备及其应用 (Preparation and application of Cirsium setosum carbon quantum dots (nanoparticles/nanoparticles) ) 是由 屈会化 赵琰 王庆国 孔慧 罗娟 熊威 朱雅凡 张越 成金俊 鲁放 孙紫薇 张 于 2018-06-22 设计创作，主要内容包括：本发明涉及从小蓟炭中提取分离纯化出的量子点(纳米颗粒/纳米粒子)的方法,该方法包括：小蓟炭,经溶剂萃取或者提取,再用分子量大于1000Da的透析袋透析,或者超滤,得到小蓟炭量子点,该量子点在紫外灯365nm下呈蓝色荧光、透射电镜测量粒径＜100nm,主要由C、H、O和N等元素构成；本发明还涉及此量子点在医药领域的应用,通过注射、口服或外用等途径给药后产生的止血、镇痛、镇静、抗炎、抗过敏、抗氧化、解热、解痉、降糖、降压、对抗肿瘤化疗药物的副反应等。(The invention relates to a method for extracting, separating and purifying quantum dots (nano particles/nano particles) from Cirsium setosum charcoal, which comprises the following steps: extracting herba Cephalanoploris carbon with solvent, dialyzing with dialysis bag with molecular weight greater than 1000Da, or ultrafiltering to obtain herba Cephalanoploris carbon quantum dot which exhibits blue fluorescence under ultraviolet lamp 365nm and has particle diameter less than 100nm measured by transmission electron microscope, and mainly comprises C, H, O and N; the invention also relates to the application of the quantum dot in the field of medicine, and the quantum dot can be used for stopping bleeding, relieving pain, tranquilizing, resisting inflammation, resisting allergy, resisting oxidation, relieving fever, relieving spasm, reducing blood sugar, reducing blood pressure, resisting side reaction of tumor chemotherapy drugs and the like after being administrated by injection, oral administration or external application and other ways.)

1. A method for extracting and purifying quantum dots from herba Cephalanoploris charcoal.

The preparation method of herba Cephalanoploris charcoal comprises parching, and calcining. Extracting with solvent, dialyzing with dialysis bag with molecular weight of 1-10 KDa, and collecting the fraction retained in the dialysis bag, or ultrafiltering with ultrafiltration tube of 10KD to obtain filtrate. The quantum dots have the following characteristics: under an ultraviolet lamp of 365nm, the particle size is 1-100nm measured by a transmission electron microscope, and the particle size mainly comprises C, O and N elements.

2. Application of herba Cephalanoploris carbon quantum dots in the field of medicine is provided.

Including but not limited to hemostasis, analgesia, sedation, anti-inflammation, anti-allergy, anti-oxidation, antipyretic, spasmolysis, hypoglycemic, antihypertensive, anti-side effects of tumor chemotherapy drugs.

3. The method for preparing Cirsium setosum charcoal according to claim 1, wherein the temperature of the dry distillation method is 100-1500 ℃. The extraction solvent is one or more of water, methanol, ethanol, ethyl acetate, ethyl formate, methyl acetate, acetone, diethyl ether, chloroform, and petroleum ether.

4. The method for preparing Cirsium setosum charcoal according to claim 3, wherein the temperature of the dry distillation method is 200-500 ℃.

5. The quantum dot of claim 1 having a particle size of 1-20 nm. The elemental composition of quantum dots includes, but is not limited to C, H, O and the N element.

6. The use of claim 2, wherein the dosage form is injection, powder for injection, oral liquid, hard capsule, soft capsule, tablet, suppository, water-paste pill, honeyed pill, concentrated pill, ointment, powder, emulsion, paste, and liniment.

7. The use according to claim 2 for the treatment or prevention of a condition which is: various bleeding diseases, blood vessel burst, cerebral hemorrhage, liver hemorrhage, pulmonary hemorrhage, renal hemorrhage, splenic hemorrhage, biliary hemorrhage, retinal hemorrhage, nasal hemorrhage, oral hemorrhage, digestive hemorrhage, gastric hemorrhage, intestinal hemorrhage, rectal hemorrhage, puerperal hemorrhage, metrorrhagia, postoperative hemorrhage, traumatic hemorrhage, etc.; pain due to internal or external injury, cancer pain, headache, eye pain, ear pain, nasal pain, oral pain, skin pain, heart pain, liver pain, stomach pain, lung pain, visceral pain, limb pain; allergic rhinitis, asthma, eczema, urticaria, purpura, drug allergy, food allergy, ultraviolet allergy, allergic constitution, various allergies and anaphylactoid; mania, insomnia, anxiety, depression; fever, high fever of unknown cause, low fever; burn, scald, convulsion, epilepsy, hypertension, diabetes, headache, nausea, emesis, edema, and apoplexy caused by tumor chemotherapy medicine.

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to preparation of Cirsium setosum charcoal quantum dots and application of the Cirsium setosum charcoal quantum dots in the field of medicines.

Background

The herba Cephalanoploris charcoal is carbide prepared from herba Cephalanoploris. The book records "the color changes from red to black. Herba Cephalanoploris is sweet, bitter and cool in nature. Meridian tropism enters heart and liver meridians. It is mainly used to cool blood, dispel stasis and stop bleeding. It is used to treat hematemesis, epistaxis, hematuria, stranguria with blood, hematochezia, metrorrhagia, acute infectious hepatitis, traumatic hemorrhage, furuncle, and carbuncle. The preparation method mainly comprises the following steps: 1. putting clean field thistle sections into a pot, frying until the field thistle sections are brownish black, paying attention to the storage property, spraying a little clear water, taking out and airing.

2. High temperature calcined charcoal deposit property

Herba Cephalanoploris mainly contains linarin, rutin, protocatechuic acid, chlorogenic acid (chlorogenic acid), caffeic acid, and potassium chloride.

Disclosure of Invention

The invention extracts and separates quantum dots (nano particles/nano particles) from the common thistle charcoal for the first time, and discovers that the common thistle charcoal quantum dots have strong biological effects of hemostasis, sedation, analgesia, anti-inflammation and the like for the first time.

The technical scheme is as follows: the invention provides a method for extracting and separating carbon quantum dots from Cirsium setosum charcoal. The invention also provides the biological activity of the Cirsium setosum charcoal quantum dots and the application thereof in the field of medicine.

The preparation method specifically comprises the following steps: calcining to prepare the Cirsium setosum charcoal, extracting quantum dots from the Cirsium setosum charcoal by using a solvent, and further separating and purifying by dialysis, ultrafiltration, chromatography and other technical methods to obtain the high-purity carbon quantum dots.

Drawings

FIG. 1 is a transmission electron microscope image of Cirsium setosum charcoal quantum dots

FIG. 2 is a graph of ultraviolet, fluorescence and infrared spectra of Cirsium setosum charcoal quantum dots

FIG. 3 is a chart of hemostatic effect of Cirsium setosum charcoal quantum dots

Detailed Description