阅读说明：本技术 一种全自动程控型模拟仿生胃肠消化装置 (Full-automatic program-controlled simulated bionic gastrointestinal digestion device ) 是由 田金虎 叶兴乾 刘东红 陈士国 郑瑜雪 于 2019-09-12 设计创作，主要内容包括：本发明公开了全自动程控型模拟仿生胃肠消化装置,属于食品科学实验技术领域。该装置包括无级变速电机、传动设备、软质反应罐、固定装置、蠕动泵、阀门、蠕动滚轮、隔热中空箱、酸碱溶液导管、加热系统、有机玻璃面板、酶溶液导管、封口盖、电脑程控终端、pH/温度探头。本发明提供了一种可实时监控,方便取样的人体全自动仿生胃肠模拟消化设备。(The invention discloses a full-automatic program-controlled simulated bionic gastrointestinal digestion device, and belongs to the technical field of food science experiments. The device comprises a stepless speed change motor, transmission equipment, a soft reaction tank, a fixing device, a peristaltic pump, a valve, a peristaltic roller, a heat insulation hollow box, an acid-base solution conduit, a heating system, an organic glass panel, an enzyme solution conduit, a sealing cover, a computer program control terminal and a pH/temperature probe. The invention provides a full-automatic bionic gastrointestinal simulated digestion device for a human body, which can be monitored in real time and is convenient for sampling.)

1. The utility model provides a bionical stomach and intestine digestion device of full-automatic programme-controlled type simulation, its characterized in that, equipment includes infinitely variable speed motor (1), transmission (2), belt, soft retort (3), fixing device (4), peristaltic pump (5), valve (6), peristaltic roller (7), thermal-insulated cavity case, acid-base solution pipe (10), heating system, organic glass panel (12), enzyme solution pipe (13), closing cap (14), computer programme-controlled terminal (15), pH/temperature probe. The computer program control terminal (15) and the peristaltic pump (5) are arranged on the left side of an organic glass panel (12), the organic glass panel (12) is located above the heat-insulation hollow box, the stepless speed change motor (1) is arranged at the bottom of the heat-insulation hollow box, the heating system and the transmission equipment (2) are arranged in the heat-insulation hollow box, and the transmission equipment (2) is connected with the stepless speed change motor (1) through a belt; organic glass panel (12) and thermal-insulated cavity case upper portion are equipped with the hole, and the upper end through-penetration hole of soft retort (3) is fixed through fixing device (4), closing cap (14) set up in the last port of soft retort (3), valve (6) set up in the bottom of soft retort (3), pH temperature probe inlays in the inboard of soft retort (3). The peristaltic rollers (7) are embedded on the belt and distributed around the soft reaction tank (3), and the bottom of the peristaltic rollers (7) is fixed with the heat-insulating hollow box through screws. The outlet of the acid-base solution conduit (10) and the outlet of the enzyme solution conduit (13) are communicated with the upper port of the soft reaction tank (3), and the enzyme solution conduit (13) and the acid-base solution conduit (10) are connected with the peristaltic pump (5) in a rolling manner.

The peristaltic pump (5), the inorganic variable speed motor (1), the heating system and the pH/temperature probe are respectively connected with the computer program control terminal (15).

2. The bionic gastrointestinal simulated digestion equipment as claimed in claim 1, wherein the soft reaction tank (3) is made of silica gel, the middle of the soft reaction tank (3) is round, the two ends of the soft reaction tank are gradually narrowed, and the two ends of the soft reaction tank are both open.

3. The biomimetic gastrointestinal simulated digestion apparatus according to claim 1, wherein the heating system comprises a heating device (11) and a temperature sensing probe, the heating device (11) is fixed on the wall of the heat-insulating hollow box through a screw, and the temperature sensing probe is embedded in the inner wall of the heat-insulating hollow box.

Technical Field

The invention belongs to the technical field of food science experiments, and particularly relates to a full-automatic program-controlled simulated bionic gastrointestinal digestion device.

Background

In vitro digestion models have been widely used in various fields such as medicine, nutrition, toxicology, physiology, etc., and in various industries such as food, health products, medicines, etc. Used for researching physical, chemical and biological changes of foods/medicines and the like in the digestive process of a human body. Particularly in the field of food science, the in-vitro simulated digestion model can be used for researching the interaction of food in the digestion process, the structural change of food in different scales in the digestion process, the utilization efficiency of nutrient substances, the release rule of functional components, the evaluation of the glycemic index of carbohydrate and the like. At present, a human body in-vitro digestion model is gradually complicated, the acceptance of relevant research on the utilization of the in-vitro digestion model is higher and higher at home and abroad, but compared with in-vivo conditions, the existing gastrointestinal tract model is still relatively simple and is in a blank state in the aspects of feedback mechanism establishment, accurate hormone control and the like, so that the correlation between most experimental data and the result of in-vivo experiments is poor, and the main reason is that the pH, digestive enzymes, temperature, peristalsis and the like in the digestion process cannot be accurately regulated and controlled in the simulated digestion process.

Disclosure of Invention

In order to solve the problems that the temperature, the pH value, the peristalsis and the like cannot be accurately regulated and controlled by the conventional gastrointestinal simulated digestion equipment, the invention provides a full-automatic program-controlled simulated bionic gastrointestinal digestion device based on the conventional human gastrointestinal simulated digestion research foundation, so that the full-automatic program-controlled human bionic gastrointestinal digestion device is realized.

In order to achieve the purpose, the technical scheme of the invention is as follows: the utility model provides a bionical stomach and intestine digestion device of full-automatic programme-controlled type simulation, equipment includes infinitely variable speed motor, transmission, belt, soft retort, fixing device, peristaltic pump, valve, peristaltic roller, thermal-insulated cavity case, acid-base solution pipe, heating system, organic glass panel, enzyme solution pipe, closing cap, computer program control terminal, pH/temperature probe. The computer program control terminal and the peristaltic pump are arranged on the left side of an organic glass panel, the organic glass panel is positioned above the heat-insulating hollow box, the stepless speed change motor is arranged at the bottom of the heat-insulating hollow box, the heating system and the transmission equipment are arranged in the heat-insulating hollow box, and the transmission equipment is connected with the stepless speed change motor through a belt; organic glass panel and thermal-insulated cavity case upper portion are equipped with the hole, and the hole is run through to the upper end of soft retort, and is fixed through fixing device, the seal closure sets up in the last port of soft retort, the valve sets up in the bottom of soft retort, pH temperature probe inlays in the inboard of soft retort. The peristaltic rollers are embedded on the belt and distributed around the soft reaction tank, and the bottom of the peristaltic rollers is fixed with the heat-insulating hollow box through screws. And the outlet of the acid-base solution conduit and the outlet of the enzyme solution conduit are communicated with the upper port of the soft reaction tank, and the enzyme solution conduit and the acid-base solution conduit are connected with the peristaltic pump in a rolling manner.

The peristaltic pump, the inorganic variable speed motor, the heating system and the pH/temperature probe are respectively connected with the computer program control terminal.

Further, soft retort comprises the silica gel material, soft retort's centre is circular, and both ends are the taper, and both ends are the opening form.

Further, heating system includes firing equipment and temperature-sensing probe, firing equipment passes through the fix with screw on the wall of thermal-insulated cavity case, temperature-sensing probe inlays in the inner wall of thermal-insulated cavity case.

Compared with the prior art, the invention has the following beneficial effects: according to the invention, the silica gel material with good elasticity is used as the wall material of the reactor, and the temperature and pH sensor probe is embedded in the inner wall of the silica gel, so that compared with the traditional magnetic stirring and conventional silica gel wall material without probe, the creep state can be simulated more truly, and the key parameters in the digestion process can be simulated more truly. Compare in traditional stomach intestine simulation digestion equipment, this equipment can realize the accurate convenient regulation and control of temperature, stomach intestine wriggling speed, pH, enzyme concentration through computer program control terminal to the realization is to the accurate regulation and control of whole simulation digestion process. The device has the characteristics of real-time monitoring and simple and convenient operation.

Drawings

FIG. 1 is a schematic diagram of the mechanism of the in vitro simulated digestion unit for human gastrointestinal tract of the invention, wherein (A) is a front view, (B) is a rear view, (C) is a top view, and (D) is a perspective view.

Wherein: 1 stepless speed change motor, 2 transmission equipment, 3 soft reaction tank, 4 fixing device, 5 peristaltic pump, 6 valve, 7 peristaltic roller, 8 thermal insulation inner wall, 9 stainless steel shell, 10 acid-base solution conduit, 11 heating equipment, 12 organic glass panel, 13 enzyme solution conduit, 14 sealing cover, 15 computer program control terminal.

Detailed Description

As shown in fig. 1, which is a schematic diagram of a mechanism of a human gastrointestinal tract in vitro simulation digestion unit of the invention, the device comprises a stepless speed change motor 1, a transmission device 2, a belt, a soft reaction tank 3, a fixing device 4, a peristaltic pump 5, a valve 6, a peristaltic roller 7, a heat insulation hollow box, an acid-base solution conduit 10, a heating system, an organic glass panel 12, an enzyme solution conduit 13, a sealing cover 14, a computer program control terminal 15 and a pH/temperature probe. The computer program control terminal 15 and the peristaltic pump 5 are arranged on the organic glass panel 12; the organic glass panel 12 is positioned above the heat-insulating hollow box, the stepless speed change motor 1 is arranged at the bottom of the heat-insulating hollow box, the heating system and the transmission equipment 2 are arranged in the heat-insulating hollow box, and the transmission equipment 2 is connected with the stepless speed change motor 1 through a belt; the heat-insulating hollow box is composed of an inner layer and an outer layer and comprises a heat-insulating inner wall 8 and a stainless steel shell 9. The technical personnel in the field only need be in organic glass panel 12 sets up the hole with thermal-insulated cavity case upper portion, with the upper end through-penetration hole of soft retort 3, fixed through fixing device 4, fixing device 4 is detachable buckle, can realize the quick thorough cleaning to soft retort. The digestion process can be simulated by pouring the materials to be subjected to simulated digestion into the soft reaction tank 3 from the upper port of the soft reaction tank 3 by the personnel in the field, the sealing cover 14 is arranged at the upper port of the soft reaction tank 3, the valve 6 is arranged at the bottom of the soft reaction tank 3, and the valve 6 is also a detachable buckle, so that the soft reaction tank 3 can be quickly and thoroughly cleaned. The pH/temperature probe is embedded in the inner side of the soft reaction tank 3 and is connected with the computer program control terminal 15, so that the real-time monitoring of the temperature/pH in the soft reaction tank 3 is realized. Peristaltic rollers 7 inlay on the belt, distribute in around soft retort 3, the bottom and the thermal-insulated cavity case of peristaltic rollers 7 pass through the fix with screw, and at infinitely variable speed motor 1's rotation in-process, through the regular rotation of peristaltic rollers 7, the extrusion soft retort 3 can simulate the stomach and intestine wriggling. The skilled person only needs to connect the outlet of the acid-base solution conduit 10 and the outlet of the enzyme solution conduit 13 with the upper port of the soft reaction tank 3, and the enzyme solution conduit 13 and the acid-base solution conduit 10 are connected with the peristaltic pump 5 by rolling, so that the acid-base solution and the enzyme solution are pumped into the soft reaction tank 3.

The peristaltic pump 5 is connected with a computer program control terminal 15, and signals are fed back to the enzyme solution conduit 13 and the acid-base solution conduit 10 through a control instruction of the computer program control terminal 15, so that the accurate control of the adding amount of acid-base and enzyme is directly realized.

The inorganic variable speed motor 1 is connected with the computer program control terminal 15, and the rotation speed of the peristaltic roller 7 is adjusted by giving an instruction at the computer program control terminal 15, so that simulation of different extrusion pressure degrees of the stomach and the small intestine is realized.

The heating system is connected with the computer program control terminal 15, so that the real-time monitoring of the temperature/pH value in the soft reaction tank 3 is realized.

The soft reaction tank 3 is made of silica gel, the middle of the soft reaction tank 3 is circular, two ends of the soft reaction tank are gradually narrowed, and two ends of the soft reaction tank are both open. The soft retort 3 can contain liquid, semiliquid and solid digestate therein.

Heating system includes firing equipment 11 and temperature-sensing probe, firing equipment 11 passes through the fix with screw on the wall of thermal-insulated cavity case, temperature-sensing probe inlays in the inner wall of thermal-insulated cavity case. The temperature in the soft reaction tank 3 is kept constant by controlling the temperature of the heat-insulating hollow box. When the temperature is lower than the set temperature, the heating system is automatically started, and when the temperature is higher than the set temperature, the hot air heating system stops working.

The working process of the invention for simulating gastrointestinal digestion of human bodies is as follows: preheating a heat-insulating hollow box to a set temperature, opening a sealing cover 14 at the upper end of a soft reaction tank 3, loading a sample into the soft reaction tank 3, starting a peristaltic pump 5, pumping acid liquid and gastric digestive enzyme liquid into the soft reaction tank 3, and starting an inorganic variable speed motor 1 to simulate gastric peristalsis. In the operation process, a plurality of pH/temperature sensors embedded in the inner wall of the soft reaction tank 3 monitor the digestion liquid environment in real time. And if the pH value of the digestion solution is lower than the minimum value or the maximum value, starting the acid-alkali solution peristaltic pump, injecting diluted alkali solution or acid solution into the soft reaction tank 3, and stopping the acid-alkali solution peristaltic pump after the target value is reached. The temperature in the soft reaction tank 3 is controlled by the hollow heat insulation box and the heating system in the process, so that the temperature of the gastrointestinal tract of the human body is simulated to the maximum extent. After the sample is fully reacted with the gastric reaction liquid in the soft reaction tank 3, the pH value of the solution is adjusted to the small intestine digestion environment through the peristaltic pump 5, then quantitative small intestine digestive enzymes such as pancreatin and the like are pumped in through the peristaltic pump 5, the rotating speed of the roller is adjusted, and the small intestine digestion stage is simulated. In the whole simulation reaction process, the sealing cover at the upper end of the soft reaction tank is opened to perform sampling analysis at any time point, so that the analysis of the physicochemical and biological characteristics of food in the simulation digestion process is completed.