阅读说明：本技术 一种多标志物检测的磁微粒发光微流控芯片以及检测装置 (Multi-marker detection magnetic particle luminous micro-fluidic chip and detection device ) 是由 王东 江荣香 李泉 于 2019-10-11 设计创作，主要内容包括：本发明属于微流控芯片发光免疫检测技术领域,尤其涉及一种多标志物检测的磁微粒发光微流控芯片以及检测装置,芯片包括：顶板,顶板上设有至少一个加样部、与加样部相互连通的混合区、以及设在混合区中的多个标记配体；底板,底板包括与混合区相互连通的导流区、与导流区相互连通的反应区、多个与反应区相互连通的检测区、与检测区相互连通的清洗液存储部和发光液存储部；反应区设有多个磁颗粒配体,各个磁颗粒配体的磁颗粒和配体均不同；清洗液存储部内部设有清洗液,发光液存储部内存储有发光液；设置在顶板上并用于驱动加样部中的样本流经混合区的气泵。由于检测区设有多个,能够避免磁颗粒相互交叉影响,大大提高了检测结果的准确性。(The invention belongs to the technical field of micro-fluidic chip luminescence immunodetection, and particularly relates to a multi-marker detection magnetic particle luminescence micro-fluidic chip and a detection device, wherein the chip comprises: the top plate is provided with at least one sample adding part, a mixing region mutually communicated with the sample adding part and a plurality of labeled ligands arranged in the mixing region; the bottom plate comprises a flow guide area communicated with the mixing area, a reaction area communicated with the flow guide area, a plurality of detection areas communicated with the reaction area, a cleaning liquid storage part and a luminous liquid storage part which are communicated with the detection areas; the reaction zone is provided with a plurality of magnetic particle ligands, and the magnetic particles and the ligands of each magnetic particle ligand are different; the cleaning liquid storage part is internally provided with cleaning liquid, and the luminous liquid storage part stores luminous liquid; and the air pump is arranged on the top plate and used for driving the sample in the sample adding part to flow through the mixing area. Because the detection zone is equipped with a plurality ofly, can avoid magnetic particle intercrossing to influence, improved the accuracy of testing result greatly.)

1. A magnetic particle luminous micro-fluidic chip for multi-marker detection is characterized by comprising:

the top plate is provided with at least one sample adding part, a mixing region communicated with the sample adding part and a plurality of labeling ligands arranged in the mixing region, and the labeling ligands are different from one another;

a bottom plate including a flow guide region interconnected with the mixing region, a reaction region interconnected with the flow guide region, a plurality of detection regions interconnected with the reaction region, a cleaning liquid storage portion and a luminescent liquid storage portion interconnected with the detection regions;

the reaction zone is provided with a plurality of magnetic particle ligands, the magnetic particle ligands comprise magnetic particles and ligands, and the magnetic particles and the ligands of each magnetic particle ligand are different;

the cleaning liquid storage part is internally provided with cleaning liquid, and the luminous liquid storage part is internally provided with luminous liquid;

and the air pump is arranged on the top plate and used for driving the sample in the sample adding part to flow through the mixing area.

2. The magnetic particle luminescent microfluidic chip of claim 1, wherein each magnetic particle differs in at least one of nuclear to mass ratio, mass, and volume.

3. The magnetic particle luminescent microfluidic chip according to claim 1, wherein a positive electrode and a negative electrode are disposed on both sides of the detection zone channel, respectively, and isoelectric points of the magnetic particles are different.

4. The magnetic particle luminescent microfluidic chip according to claim 1, wherein the mixing region is provided with a labeled ligand storage portion, and a labeled ligand is stored in the labeled ligand storage portion.

5. The magnetic particle luminescent microfluidic chip of claim 4, wherein the number of the sample addition parts and the number of the labeled ligand storage parts are consistent with each other.

6. The magnetic particle luminescent microfluidic chip of claim 1, wherein the sample application part comprises a sample application port and a cover for opening or closing the sample application port, and the sample application part further comprises a rubber ring disposed on the sample application port.

7. The magnetic particle luminescent microfluidic chip of claim 1, wherein the flow guide region has a groove therein with a height lower than the bottom wall of the reaction region and a flow guide portion disposed on the groove and connected to the reaction region.

8. The magnetic particle luminescent microfluidic chip according to any one of claims 1 to 7, wherein the detection region comprises a washing region and a light emitting region which are communicated with each other, the washing region is provided with at least one, and the light emitting region is provided with a plurality of light emitting regions.

9. The magnetic particle luminescent microfluidic chip of claim 8, wherein the number of the cleaning regions and the number of the light emitting regions are consistent with each other, and the cleaning regions are arranged corresponding to the light emitting regions.

10. A multi-marker detection magnetic particle luminescence microfluidic detection device, comprising:

the magnetic particle luminescent microfluidic chip of any one of claims 1 to 9;

the magnet unit is used for driving the magnetic particles to move;

a pressing unit for pressing the cleaning liquid storage part, the luminous liquid storage part and the air pump;

a detection unit for detecting a luminescence signal in the detection zone.

Technical Field

The invention belongs to the technical field of micro-fluidic chip luminescence immunodetection, and particularly relates to a multi-marker detection magnetic particle luminescence micro-fluidic chip and a detection device.

Background

Currently, there are two major trends In Vitro Diagnostics (IVD): one is automatic and integrated, namely, the high-precision disease analysis and diagnosis is realized by utilizing full-automatic and high-sensitivity large-scale instruments and equipment of a central laboratory matched with a large-scale hospital; the other type of miniaturization and bedside miniaturization is realized, namely, the rapid analysis and diagnosis on site is realized through handheld small simple equipment. However, small hospitals are not capital intensive, have a small sample size, and are not suitable for purchasing expensive large equipment. Therefore, most of the rapid detection equipment adopted by hospitals at the present stage mainly comprises test strips and corollary equipment thereof, but the test strips can only realize qualitative or semi-quantitative detection, and have the advantages of low detection sensitivity, poor specificity, poor repeatability and obvious interference. Due to the fact that China has a large population, aging is aggravated, the disease incidence is increased sharply, and dependence on large hospitals is overwhelmed. Therefore, the development of a rapid detection method and equipment which are simple and convenient to operate, high in sensitivity, good in repeatability and accurate in quantification becomes urgent.

Chemiluminescence refers to the phenomenon in which chemical energy is converted into light energy by reaction intermediates, reaction products or an additional luminescent reagent in a chemical reaction process. Compared with fluorescence and absorption light, chemiluminescence has no interference of external excitation light source background signals, has small cross interference, and has the advantages of high sensitivity, wide linear range and the like. The chemiluminescence analysis established by the method is widely applied to the fields of clinical diagnosis and the like. The chemiluminescence apparatus is a main large-scale IVD analysis and detection device.

The micro-fluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the biological, chemical and medical analysis process into a micron-scale chip, and automatically completes the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine, etc., it has been developed into a research field with multiple interdisciplinary disciplines of biology, chemistry, medicine, fluid, material, machinery, etc., and is applied to the fields of biomedical research, biochemical detection, judicial appraisal, etc.

The detection area of the existing microfluidic chip is only provided with one detection area, when the labeled ligand needed by the sample is different, the sample enters a reaction area reacted with the magnetic particle ligand after being mixed with the labeled ligand, and then enters the detection area for quantitatively analyzing and detecting the sample after the reaction. And when the sample got into the detection zone, need outside magnet to drive the magnetic particle of magnetic particle ligand and remove to the detection zone, because the volume of different magnetic particles, weight and nuclear matter are than different, and magnet is also different to the gathering adsorption of different magnetic particles, and the magnetic particle that volume, weight and nuclear matter are than big is relatively fast for the magnetic particle moving speed that volume, weight and nuclear matter are than little, utilizes the moving speed of magnetic particle different for the magnetic particle gets into the detection zone in proper order. Because the detection zone is only equipped with one, the magnetic particle all gets into in this detection zone, though the translation rate of magnetic particle is different, nevertheless, especially under the very fast condition of magnet translation rate, the stroke of magnetic particle is shorter again, still causes the phenomenon of magnetic particle intercrossing influence easily, greatly reduced the accuracy of testing result.

Disclosure of Invention

The embodiment of the invention provides a magnetic particle luminous micro-fluidic chip for multi-marker detection and a detection device, aiming at solving the problems that magnetic particles are easy to cross and influence each other and the accuracy of a detection result is greatly reduced when the existing magnetic particle luminous micro-fluidic chip is used for detection.

The embodiment of the invention is realized in such a way that a magnetic particle luminous micro-fluidic chip for multi-marker detection is provided, which comprises: the top plate is provided with at least one sample adding part, a mixing region communicated with the sample adding part and a plurality of labeling ligands arranged in the mixing region, and the labeling ligands are different from one another; a bottom plate including a flow guide region interconnected with the mixing region, a reaction region interconnected with the flow guide region, a plurality of detection regions interconnected with the reaction region, a cleaning liquid storage portion and a luminescent liquid storage portion interconnected with the detection regions; the reaction zone is provided with a plurality of magnetic particle ligands, the magnetic particle ligands comprise magnetic particles and ligands, and the magnetic particles and the ligands of each magnetic particle ligand are different; the cleaning liquid storage part is internally provided with cleaning liquid, and the luminous liquid storage part is internally provided with luminous liquid; and the air pump is arranged on the top plate and used for driving the sample in the sample adding part to flow through the mixing area.

Further, at least one of the nuclear to mass ratio, the mass, and the volume of each of the magnetic particles is different.

Furthermore, a positive electrode and a negative electrode are respectively arranged on two sides of the detection area channel, and the isoelectric points of the magnetic particles are different.

Further, the mixing region is provided with a labeled ligand storage part, and a labeled ligand is stored in the labeled ligand storage part.

Further, the number of the sample addition parts and the number of the labeled ligand storing parts are equal to each other.

Furthermore, the sample adding part comprises a sample adding port and a sealing cover for opening or sealing the sample adding port, and the sample adding part also comprises a rubber ring arranged on the sample adding port.

Furthermore, a groove with the height lower than the bottom wall of the reaction area and a flow guide part which is arranged on the groove and connected with the reaction area are arranged inside the flow guide area.

Furthermore, the detection area comprises a cleaning area and a light emitting area which are communicated with each other, at least one cleaning area is arranged, and the light emitting area is provided with a plurality of light emitting areas.

Furthermore, the number of the cleaning areas and the number of the luminous areas are consistent with each other, and the cleaning areas are arranged corresponding to the luminous areas.

The invention also provides a multi-marker detection magnetic particle luminescence microfluidic detection device, which comprises: the magnetic particle light-emitting microfluidic chip as described above; the magnet unit is used for driving the magnetic particles to move; a pressing unit for pressing the cleaning liquid storage part, the luminous liquid storage part and the air pump; a detection unit for detecting a luminescence signal in the detection zone.

Compared with the prior art, the magnetic particle luminous micro-fluidic chip for multi-marker detection and the detection device provided by the invention have the beneficial effects that a sample enters from the sample adding part of the top plate, the sample and each labeled ligand are mixed in the mixing region, then enter the flow guide region, enter the reaction region of the bottom plate from the flow guide region and enter different detection regions from the reaction region, at the moment, an external magnet drives magnetic particles of the magnetic particle ligands to enter different detection regions, and the luminous detection is realized in the detection regions.

Drawings

FIG. 1 is a schematic view of a magnetic particle luminescent microfluidic chip provided with a plurality of tag ligand storage portions and luminescent regions according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a magnetic particle luminescence microfluidic chip with multiple sample loading parts, labeled ligand storage parts and luminescent regions according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of a magnetic particle luminescence microfluidic chip with a plurality of tag ligand storage portions, a washing region and a luminescent region according to an embodiment of the present invention;

fig. 4 is a schematic diagram of a magnetic particle luminescent microfluidic chip with a plurality of sample adding parts, a labeled ligand storage part, a cleaning region and a luminescent region according to an embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The invention provides a magnetic particle luminous micro-fluidic chip for multi-marker detection and a detection device, wherein a sample enters from a sample adding part 11 of a top plate 1, the sample and various labeled ligands are mixed in a mixing region 12, then enter a flow guide region 21, enter a reaction region 22 of a bottom plate 2 from the flow guide region 21, and enter different detection regions from the reaction region 22, at the moment, an external magnet drives magnetic particles of the magnetic particle ligands to enter different detection regions, and luminous detection is realized in the detection regions.