阅读说明：本技术 一种芦荟多糖的制备方法 (preparation method of aloe polysaccharide ) 是由 杨成东 于 2018-05-30 设计创作，主要内容包括：本发明公开了一种从库拉索芦荟中提取芦荟多糖的制备方法,其特征在于以下步骤：1)取干燥库拉索芦荟,粉碎,过80目筛,收集粗粉备用；2)将芦荟干粉按水料比为5：1~30：1(mL/g)加入到蒸馏水中,复水30min,调节pH7~9；3)加入0.1%~0.3%的纤维素酶,在50~110℃温度下,超声提取1~4h；4)将提取液真空抽滤,取滤液并真空旋转蒸发体积至10mL,然后加入5倍体积的无水乙醇,混合均匀,置于4℃冰箱静置12h,将其在3000r/min离心10min,即得粗多糖；5)将所得粗多糖上大孔吸附树脂柱,先用1~4倍柱体积的蒸馏水洗脱,再用40~90%的乙醇洗脱,收集乙醇洗脱液；6)回收试剂,真空干燥即得芦荟多糖。本发明制备的芦荟多糖,产品纯度高,品质好。(The invention discloses a preparation method for extracting aloe polysaccharide from aloe vera, which is characterized by comprising the following steps of 1) taking dried aloe vera, crushing the dried aloe vera, sieving the crushed aloe vera with a 80-mesh sieve, collecting coarse powder for later use, 2) adding dried aloe vera powder into distilled water according to a water-material ratio of 5: 1 ~ 30: 1 (mL/g), rehydrating the mixture for 30min, adjusting the pH value to 7 ~ 9, 3) adding 0.1% ~ 0.3% of cellulase, carrying out ultrasonic extraction for 1 ~ 4h at the temperature of 50 ~ 110 ℃, 4) carrying out vacuum filtration on an extracting solution, taking a filtrate, carrying out vacuum rotary evaporation on the filtrate until the volume reaches 10mL, then adding 5 times of absolute ethyl alcohol by volume, uniformly mixing, placing the mixture in a refrigerator at 4 ℃ for 12h, centrifuging the mixture for 10min at 3000r/min to obtain crude polysaccharide, 5) putting the obtained crude polysaccharide on a macroporous adsorption resin column, carrying out distillation and elution by using 1 ~ 4 times of column volume of water, then carrying out elution by using 40 ~ 90% of ethyl alcohol, collecting an eluent, carrying out vacuum drying the reagent to obtain the aloe polysaccharide, and drying the aloe polysaccharide to obtain a high-quality aloe polysaccharide.)

1. A preparation method for extracting aloe polysaccharide from aloe vera is characterized by comprising the following steps:

1) Pulverizing dried Aloe barbadensis Miller, sieving with 80 mesh sieve, and collecting coarse powder;

2) adding Aloe dry powder into distilled water at water-to-material ratio of 5: 1 ~ 30: 1 (mL/g), rehydrating for 30min, and adjusting pH to 7 ~ 9;

3) Adding 0.1% ~ 0.3.3% cellulase, and ultrasonic extracting at 50 ~ 110 deg.C for 1 ~ 4 h;

4) vacuum filtering the extractive solution, vacuum rotary evaporating the filtrate to volume of 10mL, adding 5 times of anhydrous ethanol, mixing, standing in 4 deg.C refrigerator for 12 hr, and centrifuging at 3000r/min for 10min to obtain crude polysaccharide;

5) loading the obtained crude polysaccharide on macroporous adsorbent resin column, eluting with 1 ~ 4 times of column volume of distilled water, eluting with 40 ~ 90% ethanol, and collecting ethanol eluate;

6) Recovering the reagent, and vacuum drying to obtain aloe polysaccharide.

2. the method for extracting aloe polysaccharides from aloe vera according to claim 1, wherein the water to material ratio of step 2) is 5: 1 ~ 30: 1 (mL/g).

3. The method of claim 1, wherein the step 3) is carried out by adding 0.1% ~ 0.3.3% cellulase at 50 ~ 110 deg.C and under ultrasound for 1 ~ 4 h.

4. the method of claim 1, wherein the macroporous adsorbent resin of step 5) is one of JAD-1000, ZJX, and HD-8 types.

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of aloe polysaccharide.

Background

aloe (Aloe vera) is a perennial evergreen fleshy herbaceous plant of the genus Aloe of the family Liliaceae, distributed in tropical and subtropical regions, and currently has over 600 varieties, and is a widely used natural medicinal plant, wherein Aloe barbadensis, Aloe arborescens, Aloe china, and the like have important edible and medicinal values. Aloe is complex in chemical composition, and mainly contains anthraquinone substances, polysaccharides, amino acids, organic acids, vitamins, minerals and microelements. The aloe polysaccharide is the main bioactive substance in aloe gel, and has multiple effects of regulating immunity, resisting bacteria, viruses, tumors and oxidation, preventing aging, preventing and treating AIDS, preventing and treating diabetes, improving immunity, etc.

At present, the extraction method of aloe polysaccharide has been reported more at home and abroad, and mainly comprises hot water extraction, microwave-assisted extraction and ultrasonic-assisted extraction, while the supercritical fluid extraction method has less reports. There is no report on the extraction of aloe polysaccharides by an enzymatic method, nor on the extraction of aloe polysaccharides by combining an enzyme with ultrasonic waves. The method combines ultrasonic-assisted cellulase-macroporous resin extraction and purification technologies to prepare the high-purity aloe polysaccharide.

disclosure of Invention

The invention aims to provide an extraction method of aloe polysaccharide, and the aloe polysaccharide has wide medicinal value.

The invention is completed by the following technical scheme, and the specific content comprises the following steps: extraction method of aloe polysaccharide

1) Pulverizing dried Aloe barbadensis Miller, sieving with 80 mesh sieve, and collecting coarse powder;

2) adding Aloe dry powder into distilled water at water-to-material ratio of 5: 1 ~ 30: 1 (mL/g), rehydrating for 30min, and adjusting pH to 7 ~ 9;

3) Adding 0.1% ~ 0.3.3% cellulase, and ultrasonic extracting at 50 ~ 110 deg.C for 1 ~ 4 h;

4) Vacuum filtering the extractive solution, vacuum rotary evaporating the filtrate to 10mL, adding 5 times volume of anhydrous ethanol, mixing, standing in 4 deg.C refrigerator for 12 hr, and centrifuging at 3000r/min for 10min to obtain crude polysaccharide;

5) Loading the obtained crude polysaccharide on macroporous adsorbent resin column, eluting with 1 ~ 4 times of column volume of distilled water, eluting with 40 ~ 90% ethanol, and collecting ethanol eluate;

6) Recovering the reagent, and vacuum drying to obtain aloe polysaccharide.

The water-material ratio in the step 2) is 5: 1 ~ 30: 1 (mL/g).

Adding 0.1% ~ 0.3.3% cellulase in the step 3), and performing ultrasonic treatment at 50 ~ 110 ℃ for 1 ~ 4 h.

The macroporous adsorption resin in the step 5) is one of JAD-1000, ZJX and HD-8 types.

The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.

the specific implementation mode is as follows: