阅读说明：本技术 一种磁性筛板及其制备方法和使用方法 (Magnetic sieve plate and preparation method and use method thereof ) 是由 严义勇 邓炀 王嘉欣 马红圳 金虹 于 2019-09-18 设计创作，主要内容包括：本发明公开了一种磁性筛板及其制备方法和使用方法。本发明的磁性筛板由多种材料制成,其中至少一种材料为非永磁性材料,且至少一种材料为高分子材料。在非磁场环境中磁性筛板无磁性,与普通筛板无异,满足常见筛板的所有过滤功能；而在外置磁场中,磁性筛板产生磁性,能够被磁力操控,在外壳内进行移动,实现在外壳空间中不同位置的过滤功能,或者实现截留大颗粒定向移动,或者实现承载滤液空间的大小调控,对流体操控、自动化检测过程具有重要意义。(The invention discloses a magnetic sieve plate and a preparation method and a use method thereof. The magnetic sieve plate is made of various materials, wherein at least one material is a non-permanent magnetic material, and at least one material is a high polymer material. The magnetic sieve plate has no magnetism in a non-magnetic field environment, has no difference with a common sieve plate, and meets all filtering functions of the common sieve plate; in an external magnetic field, the magnetic sieve plate generates magnetism and can be controlled by magnetic force to move in the shell, so that the filtering function of different positions in the space of the shell is realized, or the directional movement of large particles is intercepted, or the size regulation and control of a filtrate bearing space are realized, and the device has important significance on fluid control and automatic detection processes.)

1. A magnetic sieve plate is characterized by being made of multiple materials, wherein at least one material is a non-permanent magnetic material, and at least one material is a high polymer material.

2. The magnetic screen of claim 1, wherein the non-permanent magnetic material comprises at least one of an iron-nickel alloy, an iron-cobalt alloy, an iron-aluminum alloy, and an iron oxide.

3. The magnetic sieve plate of claim 1, wherein the magnetic sieve plate has a thickness of 1nm to 1 cm; the magnetic sieve plate is provided with sieve pores which are uniformly distributed or non-uniformly distributed; the aperture of the sieve pore is 0.1-800 μm.

4. The method for preparing a magnetic sieve plate according to claim 1, wherein the method is method one or method two or method three;

the first method specifically comprises the following steps: (1) preparing magnetic microspheres by taking a non-permanent magnetic material and a high polymer material as raw materials; (2) stacking the magnetic microspheres in the center of the porous metal-based filter plate, and performing compression molding by using a press;

the second method specifically comprises the following steps: (1) providing a sintering sieve plate prepared from a high polymer material; (2) providing magnetic bead dispersion liquid prepared by non-permanent magnetic materials; (3) mixing the sintering sieve plate with the magnetic bead dispersion liquid, performing reflux reaction, and drying to obtain the magnetic sieve plate;

the third method specifically comprises the following steps: (1) preparing magnetic microspheres by taking a non-permanent magnetic material and a high polymer material as raw materials; (2) and placing the magnetic microspheres in a metal mold, placing the metal mold in a hot press, and performing compression molding to obtain the magnetic sieve plate.

5. The method for preparing the magnetic sieve plate of claim 4, wherein in the first method and the third method, the specific operations for preparing the magnetic microspheres by using the non-permanent magnetic material and the high molecular material as raw materials are as follows: (1) preparing magnetic beads by using a non-permanent magnetic material as a raw material; (2) and adding the magnetic beads into a reaction system of a high polymer material to prepare the synthetic magnetic microspheres.

6. The method for preparing the magnetic sieve plate of claim 5, wherein the specific operation of the step (2) is as follows: mixing the magnetic beads with styrene, polyvinylpyrrolidone and water, vacuumizing, introducing nitrogen and removing oxygen; stirring and heating, pouring benzoyl oxide after the temperature is stable, and reacting; and washing and collecting the magnetic polystyrene microspheres by using a magnet.

7. The method for preparing the magnetic sieve plate of claim 4, wherein in the third method, the specific operations for preparing the magnetic microspheres by using the non-permanent magnetic material and the high molecular material as raw materials are as follows: (1) preparing magnetic beads by using a non-permanent magnetic material as a raw material; (2) mixing the magnetic beads with a high polymer material, methacrylic acid and tetrahydrofuran, vacuumizing, introducing nitrogen and removing oxygen; stirring and heating, pouring benzoyl oxide after the temperature is stable, and reacting; washing and collecting by a magnet to obtain magnetic microspheres; the high polymer material is polyethylene.

8. The method for preparing a magnetic sieve plate of claim 5, wherein the magnetic beads prepared from the non-permanent magnetic material are prepared by the following steps: dissolving ferric chloride hexahydrate and ferrous chloride tetrahydrate respectively with ultrapure water, mixing, and uniformly swirling; and pouring the mixture into a mixed solution of ultrapure water and ammonia water, stirring, reacting, and washing to be neutral to obtain the ferroferric oxide magnetic beads.

9. The method for using the magnetic sieve plate of claim 1, wherein the magnetic sieve plate is placed in a housing, sample liquid is introduced to the magnetic sieve plate for filtration, and then an external magnetic field is applied to move the magnetic sieve plate up and down in the housing.

Technical Field

The invention relates to the technical field of biochemical detection, in particular to a magnetic sieve plate and a preparation method and a use method thereof.

Background

Disclosure of Invention

In order to make up for the defects of the prior art, the invention provides a magnetic sieve plate and a preparation method and a use method thereof.

The technical problem to be solved by the invention is realized by the following technical scheme:

a magnetic sieve plate is made of multiple materials, wherein at least one material is a non-permanent magnetic material, and at least one material is a high polymer material.

Further, the non-permanent magnetic material includes at least one of an iron-nickel alloy, an iron-cobalt alloy, an iron-aluminum alloy, and an iron oxide.

Further, the thickness of the magnetic sieve plate is 1nm-1 cm.

Furthermore, sieve pores are arranged on the magnetic sieve plate and are uniformly or non-uniformly distributed.

Furthermore, the aperture of the sieve pore is 0.1-800 μm.

The invention also provides a preparation method of the magnetic sieve plate, which is the first method, the second method or the third method;

the first method specifically comprises the following steps:

preparing magnetic microspheres by taking a non-permanent magnetic material and a high polymer material as raw materials;

stacking the magnetic microspheres in the center of the porous metal-based filter plate, and performing compression molding by using a press;

the second method specifically comprises the following steps:

providing a sintering sieve plate prepared from a high polymer material;

providing magnetic bead dispersion liquid prepared by non-permanent magnetic materials;

mixing the sintering sieve plate with the magnetic bead dispersion liquid, performing reflux reaction, and drying to obtain the magnetic sieve plate;

the third method specifically comprises the following steps:

preparing magnetic microspheres by taking a non-permanent magnetic material and a high polymer material as raw materials;

and placing the magnetic microspheres in a metal mold, placing the metal mold in a hot press, and performing compression molding to obtain the magnetic sieve plate.

Further, in the first and third methods, the specific operations of preparing the magnetic microspheres from the non-permanent magnetic material and the polymer material are as follows: (1) preparing magnetic beads by using a non-permanent magnetic material as a raw material; (2) and adding the magnetic beads into a reaction system of a high polymer material to prepare the synthetic magnetic microspheres.

Further, the specific operation of the step (2) is as follows: mixing the magnetic beads with styrene, polyvinylpyrrolidone and water, vacuumizing, introducing nitrogen and removing oxygen; stirring and heating, adding benzoyl oxide after the temperature is stable, and reacting; and washing and collecting the magnetic polystyrene microspheres by using a magnet.

Further, in the third method, the specific operation of preparing the magnetic microspheres by using the non-permanent magnetic material and the high molecular material as raw materials is as follows: (1) preparing magnetic beads by using a non-permanent magnetic material as a raw material; (2) mixing the magnetic beads with a high polymer material, methacrylic acid and tetrahydrofuran, vacuumizing, introducing nitrogen and removing oxygen; stirring and heating, adding benzoyl oxide after the temperature is stable, and reacting; washing and collecting by a magnet to obtain magnetic microspheres; the high polymer material is polyethylene.

Further, the specific operation of preparing the magnetic beads by using the non-permanent magnetic material as the raw material is as follows: dissolving ferric chloride hexahydrate and ferrous chloride tetrahydrate respectively with ultrapure water, mixing, and uniformly swirling; and pouring the mixture into a mixed solution of ultrapure water and ammonia water, stirring, reacting, and washing to be neutral to obtain the ferroferric oxide magnetic beads.

The invention also provides a using method of the magnetic sieve plate, which is characterized in that the magnetic sieve plate is arranged in a shell, sample liquid is introduced onto the magnetic sieve plate for filtration, and then an external magnetic field is applied to ensure that the magnetic sieve plate moves up and down in the shell.

The invention has the following beneficial effects:

the magnetic sieve plate is made of various materials, wherein at least one material is a non-permanent magnetic material, and at least one material is a high polymer material. The magnetic sieve plate has no magnetism in a non-magnetic field environment, has no difference with a common sieve plate, and meets all filtering functions of the common sieve plate; in an external magnetic field, the magnetic sieve plate generates magnetism and can be controlled by magnetic force to move in the shell, so that the filtering function of different positions in the space of the shell is realized, or large particles are intercepted to move directionally, or the size of a filtrate bearing space is regulated, or the space transfer of an adsorption target object is realized, and the method has important significance on fluid control and automatic detection processes.

The preparation method of the magnetic sieve plate is simple, and the prepared magnetic sieve plate has small aperture which can reach 3 microns.

The magnetic sieve plate has uniform magnetic distribution, the magnetic field does not exist at a position of one external magnet, and the filtering effect is better.

The magnetic sieve plate can process functional groups, realize the selective adsorption of large and small molecules such as compounds, DNA, proteins and the like, and realize the physical separation of large particles, the molecular level separation of small and large molecules and the regulation and control of the hydrophilic and hydrophobic functions of the sieve plate.

Detailed Description

In a first aspect, the present invention provides a magnetic sieve plate, which is made of multiple materials, wherein at least one material is a non-permanent magnetic material, and at least one material is a polymer material.

In the present invention, the non-permanent magnetic material includes at least one of iron-nickel alloy, iron-cobalt alloy, iron-aluminum alloy, and iron oxide, and it is understood that the non-permanent magnetic material of this embodiment includes, but is not limited to, the above listed materials, and other materials that are not listed in this embodiment but are well known to those skilled in the art may be used.

In the present invention, the kind of the polymer material is not particularly limited, and the polymer material is, for example, polystyrene or polyethylene, but is not limited thereto, and may be other materials which are not listed in the present embodiment but are known to those skilled in the art.

The existing magnetic sieve plate is usually fixed in a shell and cannot be moved and taken out in the using process; and the magnetic sieve plate generates a continuous magnetic field by itself even without applying any external magnetic field, and is generally used for adsorbing waste metal blocks, metal particles and metal scraps in a sample. This greatly limits the application of magnetic screen panels, a technical problem that has never been appreciated before by those skilled in the art. The present inventors have made further studies to solve the technical problem, and as a result, found that: the magnetic sieve plate is made of a plurality of materials, wherein at least one material is a non-permanent magnetic material, and at least one material is a high polymer material. The magnetic sieve plate has no magnetism in a non-magnetic field environment, has no difference with a common sieve plate, and meets all filtering functions of the common sieve plate; in an external magnetic field, the magnetic sieve plate generates magnetism and can be controlled by magnetic force, non-contact movement is carried out in the shell, the filtering function of different positions in the space of the shell is achieved, or large particles are intercepted and directionally moved, or the size of a filtrate bearing space is adjusted, and the method has important significance for fluid control and automatic detection processes.

In the invention, the thickness of the magnetic sieve plate is 1nm-1 cm.

In the invention, the magnetic sieve plate is provided with sieve pores which are uniformly or non-uniformly distributed.

In the present invention, the pore diameter of the mesh is 0.1 to 800. mu.m, and may be, for example, 0.1. mu.m, 10. mu.m, 50. mu.m, 100. mu.m, 200. mu.m, 300. mu.m, 400. mu.m, 500. mu.m, 600. mu.m, 700. mu.m, or 800. mu.m.

In a second aspect, the invention further provides a preparation method of the magnetic sieve plate, wherein the preparation method is the first method, the second method or the third method;

the first method specifically comprises the following steps: (1) preparing magnetic microspheres by taking a non-permanent magnetic material and a high polymer material as raw materials; (2) and stacking the magnetic microspheres in the center of the porous metal-based filter plate, and performing compression molding by using a press.

In the present invention, the steps and principles of the method for preparing the magnetic microspheres are known to the skilled person through technical manuals or conventional experimental methods, and the skilled person can adopt the existing preparation method of the magnetic microspheres in the prior art, preferably, the specific operations for preparing the magnetic microspheres by using the non-permanent magnetic material and the high molecular material as raw materials are as follows: (1) preparing magnetic beads by using a non-permanent magnetic material as a raw material; (2) and adding the magnetic beads into a reaction system of a high polymer material to prepare the synthetic magnetic microspheres.

The method steps and principles for preparing magnetic beads are known to the skilled person through technical manuals or through routine experiments, and the skilled person can adopt the existing preparation methods of magnetic beads in the prior art. Taking the non-permanent magnetic material as ferroferric oxide as an example, the specific operation for preparing the magnetic beads is as follows: dissolving ferric chloride hexahydrate and ferrous chloride tetrahydrate respectively with ultrapure water, mixing, and uniformly swirling; and pouring the mixture into a mixed solution of ultrapure water and ammonia water, stirring, reacting, and washing to be neutral to obtain the ferroferric oxide magnetic beads.

The specific operation of the step (2) is as follows: mixing the magnetic beads with styrene, polyvinylpyrrolidone and water, vacuumizing, introducing nitrogen and removing oxygen; stirring and heating, adding benzoyl oxide after the temperature is stable, and reacting; and washing and collecting the magnetic polystyrene microspheres by using a magnet.

In the first method, the magnetic microspheres are creatively stacked in the center of the porous metal-based filter plate and are pressed and formed by a press, so that the prepared magnetic sieve plate is small in pore size and uniform in magnetic distribution, a magnetic field does not exist at a position of an external magnet, and the filtering effect is better.

In the invention, the second method specifically comprises the following steps: (1) providing a sintering sieve plate prepared from a high polymer material; (2) providing magnetic bead dispersion liquid prepared by non-permanent magnetic materials; (3) and mixing the sintering sieve plate with the magnetic bead dispersion liquid, performing reflux reaction, and drying to obtain the magnetic sieve plate.

The preparation method of the sintered sieve plate in the present invention is not particularly limited, and the principle and preparation method thereof are well known to those skilled in the art, and the preparation method of the sintered sieve plate is, for example: and putting the high polymer material into a mould, putting the mould into a hot press, and performing compression molding to obtain the sintered sieve plate.

The polymer material may be polyethylene powder, but is not limited thereto, and may also be other polymer materials commonly used for preparing sintered sieve plates.

In the step (2), magnetic beads are prepared from non-permanent magnetic materials, and then the magnetic beads are dispersed in ethanol water to form magnetic bead dispersion liquid.

In the second method of the invention, firstly, the sintering sieve plate is creatively prepared by the high molecular material, and then the magnetic particles are adhered on the sintering sieve plate, the prepared magnetic sieve plate has small aperture and uniform magnetic distribution, the magnetic field does not exist at the position of a certain external magnet, and the filtering effect is better.

In the invention, the third method specifically comprises the following steps: (1) preparing magnetic microspheres by taking a non-permanent magnetic material and a high polymer material as raw materials; (2) and placing the magnetic microspheres in a metal mold, placing the metal mold in a hot press, and performing compression molding to obtain the magnetic sieve plate.

The steps and the principle of the method for preparing the magnetic microspheres are known by technical manuals or conventional experimental methods, the existing preparation method of the magnetic microspheres in the prior art can be adopted by the technical personnel in the field, and preferably, the specific operation of preparing the magnetic microspheres by taking the non-permanent magnetic material and the high molecular material as raw materials is as follows: (1) preparing magnetic beads by using a non-permanent magnetic material as a raw material; (2) and adding the magnetic beads into a reaction system of a high polymer material to prepare the synthetic magnetic microspheres. The method steps and principles for preparing magnetic beads are known to the skilled person through technical manuals or through routine experiments, and the skilled person can adopt the existing preparation methods of magnetic beads in the prior art. Taking the non-permanent magnetic material as ferroferric oxide as an example, the specific operation for preparing the magnetic beads is as follows: dissolving ferric chloride hexahydrate and ferrous chloride tetrahydrate respectively with ultrapure water, mixing, and uniformly swirling; and pouring the mixture into a mixed solution of ultrapure water and ammonia water, stirring, reacting, and washing to be neutral to obtain the ferroferric oxide magnetic beads.

The specific operation of the step (2) is as follows: mixing the magnetic beads with styrene, polyvinylpyrrolidone and water, vacuumizing, introducing nitrogen and removing oxygen; stirring and heating, adding benzoyl oxide after the temperature is stable, and reacting; and washing and collecting the magnetic polystyrene microspheres by using a magnet.

In the present invention, the size of the metal mold is not particularly limited, and may be set by those skilled in the art according to actual needs, and for example, the size of the metal mold is 20 × 0.1 cm.

The specific parameters of the operation of the hot press are not particularly limited, and can be set by a person skilled in the art according to actual needs.

In the third method, the non-permanent magnetic material and the high molecular material are used as raw materials to prepare the magnetic microspheres, then the magnetic microspheres are placed in a metal mold, the metal mold is placed in a hot press, and the magnetic sieve plate is obtained through compression molding.

As a further improvement, in the third method, the specific operations of preparing the magnetic microspheres by using the non-permanent magnetic material and the high molecular material as raw materials are as follows: (1) preparing magnetic beads by using a non-permanent magnetic material as a raw material; (2) mixing the magnetic beads with a high polymer material, methacrylic acid and tetrahydrofuran, vacuumizing, introducing nitrogen and removing oxygen; stirring and heating, pouring benzoyl oxide after the temperature is stable, and reacting; washing and collecting by a magnet to obtain magnetic microspheres; the high polymer material is polyethylene.

The method steps and principles for preparing magnetic beads are known to the skilled person through technical manuals or through routine experiments, and the skilled person can adopt the existing preparation methods of magnetic beads in the prior art. Taking the non-permanent magnetic material as ferroferric oxide as an example, the specific operation for preparing the magnetic beads is as follows: dissolving ferric chloride hexahydrate and ferrous chloride tetrahydrate respectively with ultrapure water, mixing, and uniformly swirling; and pouring the mixture into a mixed solution of ultrapure water and ammonia water, stirring, reacting, and washing to be neutral to obtain the ferroferric oxide magnetic beads.

The magnetic polyethylene microsphere modified by carboxyl can be obtained by adopting the method, the sieve plate with carboxyl groups on the surface is obtained and is used for selectively adsorbing a chemical and biological target object by taking the sieve plate as a specific modification adsorption target object, the physical separation of large particles and the separation of the molecular level of large and small molecules can be realized simultaneously, and the change of hydrophilicity and hydrophobicity can also be realized.

In a third aspect, the present invention also provides a method for using the above magnetic sieve plate, which is to place the magnetic sieve plate in a housing, introduce the sample liquid onto the magnetic sieve plate for filtration, and then apply an external magnetic field to move the magnetic sieve plate up and down in the housing.

In the present invention, the type of the housing is not specifically limited, and various housings capable of placing a sieve plate for filtration in the prior art may be adopted, for example, the housing may be a column tube, a centrifuge tube, or a silica gel hose.

Specifically, the method for using the magnetic sieve plate comprises the steps of placing the magnetic sieve plate in a shell, introducing a sample liquid onto the magnetic sieve plate for filtration, and intercepting residues on the sieve plate; then an external magnetic field is applied, and the magnetic field is moved upwards, so that the magnetic sieve plate moves upwards in the shell, and residues on the sieve plate are removed; the magnetic field is moved downwardly so that the magnetic screen deck moves downwardly in the housing.

According to the invention, the traditional mode that the magnetic sieve plate is fixed in the shell is changed, and the magnetic sieve plate can move up and down in the shell by applying an external magnetic field, so that the magnetic sieve plate is convenient to operate, strong in stability, convenient to disassemble and assemble, convenient to clean and replace, and low in use cost.

The present invention will be described in detail with reference to examples, which are only preferred embodiments of the present invention and are not intended to limit the present invention.