阅读说明：本技术 一种生物传感器及其制备方法和应用 (A kind of biosensor and its preparation method and application ) 是由 李阳 郭云建 高嵩 岳文静 张春伟 于 2019-08-28 设计创作，主要内容包括：本发明涉及生物传感器技术领域,尤其涉及一种生物传感器及其制备方法和应用。所述传感器包括底板和芯片,所述芯片为设置在该底板上的两个并联单元和金属层,所述金属层设置在两个并联单元之间；每个并联单元包括平面状的螺旋形电感和锤状电容,所述螺旋形电感的中间具有空白区域,所述锤状电容内嵌在所述空白区域中；锤状电容上引出两条导线,其中一条导线用于接地,另一条导线与所述金属层连接。底板包括衬底和SU-8高聚物钝化层,所述钝化层在传感器的检测区域形成预设厚度和预设面积的开口,以此形成设定空间溶剂的凹槽。本发明提出的射频生物传感器具有小尺寸、快检测速度、简易且低成本的加工过程、可定量检测等特点。(The present invention relates to biosensor technology fields more particularly to a kind of biosensor and its preparation method and application.The sensor includes bottom plate and chip, and the chip is two parallel units and metal layer of setting on which floor plate, and the metal layer is arranged between two parallel units；Each parallel units include planar spiral inductor and hammer shape capacitor, and the centre of the spiral inductor has white space, and the hammer shape capacitor is embedded in the white space；It hammers into shape and draws two conducting wires on shape capacitor, wherein a conducting wire is for being grounded, another conducting wire is connect with the metal layer.Bottom plate includes substrate and SU-8 high polymer passivation layer, and the passivation layer forms the opening of preset thickness and preset area in the detection zone of sensor, so as to form the groove of setting space solvent.Radio frequency biosensor proposed by the present invention have the characteristics that small size, detect fastly speed, simple and inexpensive process, can quantitative detection.)

1. a kind of biosensor, which is characterized in that including bottom plate and chip, the chip is two of setting on which floor plate Parallel units and metal layer, the metal layer are arranged between two parallel units；Each parallel units include planar spiral shell Shape inductance and hammer shape capacitor are revolved, the centre of the spiral inductor has white space, and the hammer shape capacitor is embedded in the sky In white region；Two conducting wires are drawn on the hammer shape capacitor, wherein a conducting wire is used to be grounded, another conducting wire and the metal Layer connection, to realize being connected in parallel between two parallel units, the hammer shape capacitor refers to a square capacitance and its two The structure for the likeness in form " hammer " that the conducting wire of side connection is formed.

2. biosensor as described in claim 1, which is characterized in that the material of the metal layer includes: noble metal or copper； Preferably noble metal, such as any one in gold, silver, platinum.

3. biosensor as claimed in claim 1 or 2, which is characterized in that bottom plate includes substrate and the passivation of SU-8 high polymer Layer, the passivation layer form the opening of preset thickness and preset area in the detection zone of sensor, so as to form setting space The groove of solvent.

4. biosensor as claimed in claim 3, which is characterized in that preparation method includes the following steps:

(1) with the method for plasma enhanced chemical vapor deposition, substrate surface grows one layer of gallium nitride substrate after the cleaning, with Increase the adhesiveness between metal and substrate；

(2) the method growth lower metal for the region magnetron sputtering that cooperation photoetching process is set on substrate, then removes and delusters Photoresist, lower metal has grown completion above substrate at this time；

(3) utilize photoetching process in the photoresist for the local depositing and setting height for not growing lower metal, and by high again The form of temperature heating makes photoresist refigure shape, constructs air bridge pier structure to support spiral inductor in step (7) Upper layer metal；

(4) one layer of metal seed layer is formed in entire substrate surface using the method for magnetron sputtering；

(5) using photoetching process in the area deposition photoresist for not needing growth top-level metallic；

(6) the method deposited top layer metal for utilizing magnetron sputtering, is so far completed the process by capacitor and spiral inductor, wherein capacitor It is to be directly connected to by upper layer and lower layer metal, inductance is by constituting with air bridge pier structure, in the place for not needing wire jumper The direct phase of upper layer and lower layer metal；

(7) it is coated with SU-8 photoresist on top-level metallic surface, control is coated with the film that revolving speed forms setting thickness；

(8) the SU-8 photoetching for relying on photoetching technique to require photoetching to fall to set area according to quantitative measurment in the detection zone of device Glue；

(9) device that step (8) obtains is placed in reactive ion etching system, using the mixed gas of chlorine and argon gas to device Part carry out dry etching processing to get.

5. biosensor as claimed in claim 4, which comprises the steps of:

Step 1: the substrate after cleaning being placed in grown silicon nitride film in plasma enhanced chemical vapor phase deposition reacting furnace, is sunk Product is in SiH₄And NH₃Ratio be 320:9sccm, chamber pressure 1200mTorr, environment temperature temperature be 250 DEG C, 100WRF power and It is carried out under 2000sccm air-flow；The entire processing time is fixed as 400s, growth thickness 0.2-0.4um；

Step 2: carrying out the photoetching process of bottom metal, comprising: photoresist uses negative photoresist, in photoresist spin coating process, Spin coating is carried out with the speed of 1000-3000rad/min, spin coating 40-60s generates the photoresist with a thickness of 5-7um, after spun It places the substrate on hot plate and carries out the heat treatment of 1-2min at 120-150 DEG C；It is golden using lower layer is printed on after the completion of heat treatment The photomask of metal patterns is in 200-350mJ/cm²30-90s is exposed under energy density；It is aobvious using negative photoresist after the completion of exposure Shadow liquid develops, and sufficiently finally puts after development drying and is checked under an optical microscope, is obtained with setting photoresist figure The substrate of case；

Step 3: the above-mentioned substrate with setting photoetching agent pattern being put into magnetron sputtering apparatus, is 1.0 × 10 in vacuum degree^-5-1.0×10^-8MTorr, it sinks to the bottom the rate that temperature is 20-50 DEG C, growth rate is 0.05-0.1nm/s and is grown, finally The lower metal for forming the gold element (Au) with a thickness of 3-5um is 0.8-1mol/L's using concentration after the completion of metal growth Sodium hydroxide solution removes photoresist；

Step 4: being 5-7um negative photoresist first with photoetching process building height, comprising: light to grow top-level metallic In photoresist spin coating process, spin coating is carried out with the speed of 1000-3000rad/min, spin coating 40-60s generates with a thickness of 5-7um's Photoresist places the substrate on hot plate after spun and carries out the heat treatment of 1-2min at 120-150 DEG C；After the completion of heat treatment Using photomask in 200-350mJ/cm²30-90s is exposed under energy density；After the completion of exposure, negative photo glue developing solution is utilized Develop, sufficiently finally puts after development drying and checked under an optical microscope, subsequent to refigure photoresist shape For shape to form air bridge pier structure, it is adding for 180-210s 130-160 DEG C of time that device, which is placed in the enterprising trip temperature of hot plate, to be Heat treatment；

Step 5: using magnetron sputtering method to sample carry out growth thickness be 0.1-0.4um, the plated film that film quality is gold element Operation, filming parameter are that vacuum degree is 1.0 × 10-5-1.0 × 10-8mTorr, sinks to the bottom that temperature is room temperature, growth rate is The rate of 0.05-0.1nm/s is grown；

Step 6: carry out the photoetching process of top metal, including light: photoresist uses negative photoresist, photoresist spin coating process In, spin coating is carried out with the speed of 1000-3000rad/min, spin coating 40-60s generates the photoresist with a thickness of 7-9um, spun It is placed the substrate on hot plate afterwards and carries out the heat treatment of 1-2min at 120-150 DEG C；It is utilized after the completion of heat treatment and is printed on upper layer The photomask of pattern exposes 30-90s under 200-350mJ/cm2 energy density；After the completion of exposure, developed using negative photoresist Liquid develops, and sufficiently finally puts after development drying and is checked under an optical microscope；

Step 7: the substrate with setting photoetching agent pattern being put into magnetron sputtering apparatus, is 1.0 × 10-5- in vacuum degree 1.0 × 10-8mTorr, it sinks to the bottom the rate that temperature is 20-50 DEG C, growth rate is 0.05nm/s and is grown, eventually form thickness Degree is the upper layer metal of the gold element of 2-5um, molten using the sodium hydroxide that concentration is 0.8-1mol/L after the completion of metal growth Liquid removes photoresist；

Step 8: the SU-8 photoresist for being 7-9um using photoetching process building height, to construct the groove structure of detection zone, tool Body are as follows: in photoresist spin coating process, spin coating is carried out with the speed of 1000-3000rad/min, spin coating 40-60s, generate with a thickness of The photoresist of 7-9um places the substrate on hot plate after spun and carries out the heat treatment of 1-2min at 120-150 DEG C；At heating Utilization can make detection zone that the photomask of groove structure be presented in 200-350mJ/cm after the completion of reason²It is exposed under energy density 30-90s；After the completion of exposure, the photoresist of development removal detection zone is carried out using negative photo glue developing solution, sufficiently development is dry It finally puts after dry and is checked under an optical microscope；

Step 9: being placed a sensor in plasma etching machine and carried out at dry etching using chlorine/argon plasma Reason is to get, wherein etch process conditions are as follows: etch period are as follows: 1-7min, chlorine/argon flow ratio are 20sccm:(5-20) Sccm, pressure 2-20mTorr, radio-frequency coil power are as follows: 400-600W, parallel-plate power are 100-300W, reaction chamber room temperature Degree is 20-60 DEG C.

6. biosensor as described in claim 4 or 5, which is characterized in that the material of the substrate includes GaAs, stone Any one in English, silicon.

7. biosensor as described in claim 4 or 5, which is characterized in that the method for the cleaning substrate are as follows: by substrate point Be not placed in acetone, isopropanol, in deionized water, carry out the cleaning of 3-5min, 1-3min, 3-5min respectively, then to substrate into Row drying.

8. such as application of the described in any item biosensors of claim 1-7 in medicine, environment, field of food, as blood glucose, Blood lipid monitoring；Analyze food composition, food additives；Water body, atmosphere pollution detection.

Technical field

The present invention relates to biosensor technology field more particularly to a kind of biosensor and preparation method thereof and answer With.

Background technique

It is only intended to increase understanding of the overall background of the invention for information disclosed in background of invention, without It is existing well known to persons skilled in the art so to be considered as recognizing or imply that information composition has become in any form Technology.

Biosensor is one and is interpenetrated growth by a variety of subjects such as biology, physics, chemistry, electronic technology, medicine The new and high technology got up.Bio-identification sensor-based system is usually made of bio-identification component, signal adapter and electronic system, and Core devices of the bio-identification component as the system determine accuracy, the detection duration and system cost etc. of testing result Numerous key elements.In measurement sample, biomarker corresponding to various disease (Biomarker, including it is enzyme, antibody, anti- The bioactive substances such as original, microorganism, cell, tissue, nucleic acid) corresponding electric signal shape can be converted into biosensor Formula output.Compared with traditional detection means, have accuracy high, analysis speed is fast, at low cost, favorable repeatability, operation letter It is single, numerous advantages such as specificity is strong.

It can be divided into according to signal adapter, that is, energy converter difference of biosensor: first generation electrochemical biosensor Device, second generation optical biosensor and third generation radio frequency biosensor etc..Energy converter is followed successively by electrochemical electrode, photoelectricity turns Parallel operation and radio-frequency devices etc..Wherein electrochemica biological sensor sensitivity highest is realized by adding specific enzyme on the electrode Detection to biomarker, easy to use and cost are controllable.But the introducing of foreign agent is so that sensor response is slack-off, property Energy decline and poor reliability need to be to be replaced in the period with or so half a year according to use environment difference.In addition, another is restricted The factor of electrochemical sensor application is that its electrolyte needs regular replenishment, this seriously increases after-cost.And for light For Biosensors, normal detection needs the longer stable time, and is highly prone to the influence of environment light and changes detection As a result.

Third Generation Biosensors -- radio frequency biosensor is very hot topic recent years, with processing technology Step up, the critical size of radio frequency biosensor has been reduced to micro-nano rank, structure also from traditional single layer, it is double-deck to Multilayer development.Compared to other kinds of biosensor, radio frequency biosensor has a following advantage: first, detection is steady Qualitative height, vulnerable to the restriction of use environment unlike electrochemica biological sensor, and performance can be with the passage for using the time And decline, radio frequency biosensor may remain in long-term multiple not vulnerable to such environmental effects such as ambient light photograph, temperature, humidity The stability detected in heterocycle border；Second, easy to operate, optical biosensor needs certain time to carry out stably measured environment, And radio frequency biosensor can immediately be used for quickly detecting biomarker without stablizing the time early period；Third is not necessarily to Addition foreign agent object is marked, and test solution only need to be dropped to radio frequency biosensor detection zone can be, it can be achieved that exempt from Mark analyte detection.In conclusion radio frequency biosensor has high, easy to operate, marker free detection of detection stability etc. aobvious Work advantage.

Summary of the invention

The research of the invention finds that: some Third Generation Biosensors still have device manufacturing process complexity, without standard measure The disadvantages of measurement, measurement duration.For this purpose, the invention proposes a kind of radio frequency based on spiral inductor and hammer shape capacitor is biological Sensor design is used for glucose detection, and proposes the respective sensor processing technology for realizing the sensor.

For achieving the above object, the invention discloses following technical proposals:

A kind of biosensor comprising bottom plate and chip, the chip are two and the receipts or other documents in duplicate of setting on which floor plate Member and metal layer, the metal layer are arranged between two parallel units；Each parallel units include planar spiral shape electricity Sense and hammer shape capacitor, the centre of the spiral inductor have white space, and the hammer shape capacitor is embedded in the white space In；Two conducting wires are drawn on the hammer shape capacitor, wherein a conducting wire, for being grounded, another conducting wire and the metal layer connect It connects, to realize being connected in parallel between two parallel units；The hammer shape capacitor refers to that a square capacitance and its two sides connect The structure for the likeness in form " hammer " that the conducting wire connect is formed.

The characteristics of biosensor of the present invention first is that: sensor of the invention designs two capacitors being embedded in two respectively The inside of a spiral inductance has further reduced the area of chip, and two spiral inductances and capacitor are located at intermediate conveyor The two sides of line effectively avoid the ghost effect between capacitor and capacitor, inductance and inductance in turn, and then simplify because of parasitism Effect and interference to glucose detection.

The characteristics of biosensor of the present invention second is that: the present invention substitutes existing " metal-dielectric-using hammer shape capacitor The capacitor of metal " form, and then the processing technology that must add one layer of dielectric layer in process is simplified, make to be processed into This and procedure of processing are effectively reduced.In addition, the capacitor that the present invention uses is no longer limited by " metal-dielectric-metal " The relatively low breakdown voltage of form capacitor, therefore the sensor in the present invention can be applied in high voltage and high power situation.

Compared with prior art, the present invention achieve it is following the utility model has the advantages that

(1) in order to improve the time of measuring of device, the present invention using the mixed gas of chlorine and argon gas to sensing region into Row dry etching hollows out passivation layer in device sensing region and implements to reduce its surface roughness and then promote detection time Quantitative blood glucose measurement.

(2) proposed by the present invention with small size, fastly detect speed, simple and inexpensive process, can quantitatively examine The radio frequency biosensor of survey has huge application potential in biologic medicals detection fields such as blood glucose, blood lipid monitorings.

Detailed description of the invention

The Figure of description for constituting a part of the invention is used to provide further understanding of the present invention, and of the invention shows Examples and descriptions thereof are used to explain the present invention for meaning property, does not constitute improper limitations of the present invention.

Fig. 1 is the structural schematic diagram of biosensor in the embodiment of the present invention 1.

Fig. 2 is the structural schematic diagram of biosensor in the embodiment of the present invention 2.

Fig. 3 is the flow diagram that biosensor is prepared in the embodiment of the present invention 2.

Fig. 4 is the SEM figure of the biosensor (material object) prepared in the embodiment of the present invention 2.

Fig. 5 is radio frequency glucose sensor detection system structure in the embodiment of the present invention.

Fig. 6 is the bio-sensing that the different etching time obtains in the 1-3 of the embodiment of the present invention measured with atomic force microscope Device surface roughness situation.

Fig. 7 is the detection response of biosensor under various concentration in the embodiment of the present invention 2.

The above-mentioned acceptance of the bid of attached drawing 3 note respectively indicates: 1, bottom plate；2, metal layer；3, spiral inductor；4, shape capacitor is hammered into shape；5, it leads Line；6, substrate；7, SU-8 high polymer passivation layer, 8, vector network analyzer；9, glucose solution sample；10, biosensor； 11, miniature pipettor.

Specific embodiment

It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the present invention.Unless another It indicates, all technical and scientific terms used herein has usual with general technical staff of the technical field of the invention The identical meanings of understanding.

It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root According to exemplary embodiments of the present invention.Such as, used herein, unless the context clearly indicates otherwise, otherwise singular shape Formula is also intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.

As previously mentioned, some Third Generation Biosensors still have device manufacturing process complexity, survey without standard measure The disadvantages of amount, measurement duration.Therefore, the present invention is based on spiral inductors and a kind of radio frequency bio-sensing of hammer shape capacitor design Device design to be used for glucose detection, and proposes the respective sensor processing technology for realizing the sensor.

In some typical embodiments, in the biosensor, the material of metal layer include: noble metal (as gold, Silver, platinum etc.) and copper；The preferably noble metals such as gold, silver, platinum, are not easy to be oxidized when in use.

In some typical embodiments, in the biosensor, bottom plate includes substrate and SU-8 high polymer passivation layer, The passivation layer forms the opening of preset thickness and preset area in the detection zone of sensor, so as to form setting space solvent Groove, keep the amount of the determinand detected every time constant to form quantitative detection, reduce because of detection determinand capacity every time Measurement error caused by difference.

Further, the preparation method of the biosensor includes the following steps:

(1) substrate is cleaned, surface smut is removed.

(2) one layer of gallium nitride substrate is grown in the substrate surface with the method for plasma enhanced chemical vapor deposition, To increase the adhesiveness between metal and substrate.

(3) the method growth lower metal for the region magnetron sputtering that cooperation photoetching process is set on substrate, is then removed Photoresist is removed, lower metal has grown completion above substrate at this time.

(4) utilize photoetching process in the photoresist for the local depositing and setting height for not growing lower metal, and by again The form of secondary high-temperature heating makes photoresist refigure shape, constructs air bridge pier structure to support spiral shape in step (7) The upper layer metal of inductance.

(5) one layer of metal seed layer is formed in entire substrate surface using the method for magnetron sputtering.

(6) using photoetching process in the area deposition photoresist for not needing growth top-level metallic.

(7) it is so far completed the process by capacitor and spiral inductor using the method deposited top layer metal of magnetron sputtering, Middle capacitor is directly connected to by upper layer and lower layer metal, and inductance is not need wire jumper by constituting with air bridge pier structure Descend double layer of metal to be connected directly in the localities.

(8) it is coated with SU-8 photoresist on top-level metallic surface, control is coated with the film that revolving speed forms setting thickness.

(9) the SU-8 light for relying on photoetching technique to require photoetching to fall to set area according to quantitative measurment in the detection zone of device Photoresist.

(10) device that step (9) obtains is placed in reactive ion etching system, utilizes the gaseous mixture of chlorine and argon gas Body to device carry out dry etching processing, with promoted the roughness of device detection zone so that promoted detection time to get.

With reference to Fig. 3, in some typical embodiments, the biology is prepared using the technique comprising following process parameter and is passed Sensor:

Step 1: GaAs or quartz or silicon substrate being respectively placed in acetone, isopropanol, in deionized water, carry out 3- respectively The cleaning of 5min, 1-3min, 3-5min, are then dried substrate.

Step 2: it is thin that the substrate after drying being placed in grown silicon nitride in plasma enhanced chemical vapor phase deposition reacting furnace Film is deposited on SiH₄And NH₃Ratio be 320:9sccm, chamber pressure 1200mTorr, environment temperature temperature be 250 DEG C, 100WRF function It is carried out under rate and 2000sccm air-flow.The entire processing time is fixed as 400s, growth thickness 0.2-0.4um.

Step 3: the photoetching process of bottom metal, including photoresist spin coating are carried out, is exposed, development, the processes such as inspection.Photoetching Glue uses negative photoresist, in photoresist spin coating process, carries out spin coating, spin coating 40- with the speed of 1000-3000rad/min 60s, generate with a thickness of 5-7um photoresist, placed the substrate in after spun on hot plate carry out 120-150 DEG C at 1-2min plus Heat treatment.Utilize the photomask for being printed on underlying metal patterns in 200-350mJ/cm after the completion of heat treatment²It is exposed under energy density Light 30-90s.After the completion of exposure, developed using negative photo glue developing solution, it is aobvious to be sufficiently finally placed on optics after development drying It is checked under micro mirror, obtains the substrate with setting photoetching agent pattern.

Step 4: the above-mentioned substrate with setting photoetching agent pattern being put into magnetron sputtering apparatus, is 1.0 in vacuum degree ×10^-5-1.0×10^-8MTorr, it sinks to the bottom the rate that temperature is 20-50 DEG C, growth rate is 0.05-0.1nm/s and is grown, The lower metal for eventually forming the gold element (Au) with a thickness of 3-5um is 0.8- using concentration after the completion of metal growth The sodium hydroxide solution of 1mol/L removes photoresist.

Step 5: it is 5-7um negative photoresist first with photoetching process building height to grow top-level metallic, including Photoresist spin coating exposes, development, the processes such as inspection.In photoresist spin coating process, carried out with the speed of 1000-3000rad/min Spin coating, spin coating 40-60s generate the photoresist with a thickness of 5-7um, place the substrate in after spun and carry out 120-150 DEG C on hot plate The heat treatment of lower 1-2min.Using photomask in 200-350mJ/cm after the completion of heat treatment²30- is exposed under energy density 90s.After the completion of exposure, developed using negative photo glue developing solution, is sufficiently finally placed on optical microscopy after development drying Under checked, subsequent to refigure photoresist shape forms air bridge pier structure, that device is placed in hot plate is enterprising Trip temperature is the heat treatment for 130-160 DEG C of time being 180-210s.

Step 6: using the method for magnetron sputtering be 0.1-0.4um to sample progress growth thickness, film quality is gold element Coating operation, filming parameter are that vacuum degree is 1.0 × 10^-5-1.0×10^-8MTorr, sink to the bottom that temperature is room temperature, growth rate is The rate of 0.05-0.1nm/s is grown.

Step 7: the photoetching process of top metal, including photoresist spin coating are carried out, is exposed, development, the processes such as inspection.Photoetching Glue uses negative photoresist, in photoresist spin coating process, carries out spin coating, spin coating 40- with the speed of 1000-3000rad/min 60s, generate with a thickness of 7-9um photoresist, placed the substrate in after spun on hot plate carry out 120-150 DEG C at 1-2min plus Heat treatment.Utilize the photomask for being printed on upper layer pattern in 200-350mJ/cm after the completion of heat treatment²30- is exposed under energy density 90s.After the completion of exposure, developed using negative photo glue developing solution, is sufficiently finally placed on optical microscopy after development drying Under checked.

Step 8: the substrate with setting photoetching agent pattern being put into magnetron sputtering apparatus, is 1.0 × 10 in vacuum degree^-5-1.0×10^-8MTorr, it sinks to the bottom the rate that temperature is 20-50 DEG C, growth rate is 0.05nm/s and is grown, eventually form With a thickness of the upper layer metal of the gold element (Au) of 2-5um, after the completion of metal growth, the hydrogen-oxygen for being 0.8-1mol/L using concentration Change sodium solution and removes photoresist；So far, it is completed the process by capacitor and spiral inductor, wherein capacitor is straight by upper layer and lower layer metal It connects and is formed by connecting, inductance is to descend the direct phase of double layer of metal in the localities do not need wire jumper by constituting with air bridge pier structure Even.

Step 9: in order to realize quantitative work, the groove structure of detection zone will be constructed.Height is constructed using photoetching process It for 7-9umSU-8 photoresist, including photoresist spin coating, exposes, development, the processes such as inspection.In photoresist spin coating process, with The speed of 1000-3000rad/min carries out spin coating, and spin coating 40-60s generates the photoresist with a thickness of 7-9um, will after spun Substrate is placed on the heat treatment that 1-2min at 120-150 DEG C is carried out on hot plate.Utilization can make detection zone after the completion of heat treatment The photomask of groove structure is presented in 200-350mJ/cm in domain²30-90s is exposed under energy density.After the completion of exposure, negativity is utilized Photoresist developer carries out the photoresist of development removal detection zone, sufficiently development it is dry after finally put under an optical microscope into Row checks.

Step 10: placing a sensor at progress dry method quarter in plasma etching machine using chlorine/argon plasma Erosion processing is to get, wherein etch process conditions are as follows: etch period 1-7min, chlorine/argon flow ratio are 20sccm:(5- 20) sccm, pressure 2-20mTorr, radio-frequency coil power are as follows: 400-600W, parallel-plate power are 100-300W, reaction chamber Temperature is 20-60 DEG C.

In some typical embodiments, the biosensor or the above method preparation biosensor also by with In the fields such as medicine, environment, food, such as the monitoring of blood glucose, blood lipid；Analyze food composition, food additives；Water body, atmosphere Pollution detection etc..

The characteristics of above-mentioned preparation method of the invention first is that: can not quantitative detection in contrast to other radio frequency biosensors Disadvantage sets face processing last process and form setting thickness in the detection zone of sensor using SU-8 high polymer in the present invention Long-pending opening keeps the amount of the determinand detected every time constant so as to form the groove of setting space solvent to form quantitative inspection It surveys, reduces because detecting measurement error caused by determinand capacity difference every time.

The characteristics of above-mentioned preparation method of the invention second is that: because the material for constituting biosensor is metal, and formed Metal surface it is smooth, so be difficult after glucose is contacted with metal immediately all be covered on metal surface, to reduce survey Measure speed.Keep surface more coarse metal surface etching using the mixed gas of chlorine and argon gas in the present invention, in grape Whole surface can be paved with after sugar juice contact sensor surface metal faster and implements quickly detection.

The present invention is further illustrated now in conjunction with attached drawing 1-6 and specific embodiment.