Method for preventing and controlling major diseases and pests of macadamia nut in interplanting mode

文档序号:175022 发布日期:2021-11-02 浏览:40次 中文

阅读说明:本技术 一种套种模式防控澳洲坚果主要病虫害的方法 (Method for preventing and controlling major diseases and pests of macadamia nut in interplanting mode ) 是由 陈鹏 袁瑞玲 白海东 杨建荣 冯丹 王艺璇 杜春花 于 2021-08-26 设计创作,主要内容包括:本发明属于病虫害生物防治领域,具体公开一种澳洲坚果虫害生物防治方法,于5年生澳洲坚果林中套种白魔芋,包括以下步骤:选取不小于500g的白魔芋种,要求球茎表皮粗糙,色泽较深,芽体饱满;将选取的白魔芋种置于赤霉素和6-BA中浸泡2h;澳洲坚果的种植密度为每亩25-35棵,白魔芋的播种密度为每亩200-300个,将浸泡后的白魔芋种分别于1月下旬和3月中旬,各50%播种于澳洲坚果林下,每株白魔芋底部施加不少于200g农家肥,顶部覆盖3-5cm遮阳物,本发明通过使用白魔芋和澳洲坚果套种,通过白魔芋生理特性达到防控澳洲坚果病虫害的效果,且不会像传统农药防治一样造成农药残留,通过该方法,澳洲坚果的优果率达到了80%以上。(The invention belongs to the field of biological control of diseases and pests, and particularly discloses a biological control method for macadimia nut pests, which is characterized in that white konjak is interplanted in a 5-year-old macadimia nut forest, and the biological control method comprises the following steps: selecting not less than 500g of white konjac seeds, wherein the corms are required to have rough surface, deep color and plump bud bodies; soaking the selected white konjac seeds in gibberellin and 6-BA for 2 hours; the planting density of the macadimia nuts is 25-35 seeds per mu, the seeding density of the white konjac is 200-300 seeds per mu, the soaked white konjac seeds are respectively sown under the macadimia nut forest in the last ten days of 1 month and the middle ten days of 3 months, 50% of the seeds are respectively sown under the macadimia nut forest, no less than 200g of farmyard manure is applied to the bottom of each white konjac, and 3-5cm of sunshade is covered on the top of each white konjac.)

1. The method for preventing and controlling main diseases and pests of macadimia nuts in an interplanting mode is characterized in that white konjak is interplanted in a 5-year-old macadimia nut forest, and comprises the following steps:

s1: selecting not less than 500g of white konjac seeds, wherein the corms are required to have rough surface, deep color and plump bud bodies;

s2: soaking the selected white konjac seeds in gibberellin and 6-BA for 2 hours, wherein the concentration of the gibberellin is 100mg/L, and the concentration of the 6-BA is 150 mg/L;

s3: the planting density of the macadimia nuts is 25-35 seeds per mu, the seeding density of the white konjac is 200-300 seeds per mu, the soaked white konjac seeds are respectively sown under the macadimia nut forest in the last ten days of 1 month and the middle ten days of 3 months, 50% of the seeds are respectively sown under the macadimia nut forest, the distance between the white konjac and the macadimia nut trunk is not less than 60cm, the plant spacing between the white konjac is not less than 50cm, the cultivation depth is 15-20cm, no less than 200g of farmyard manure is applied to the bottom of each white konjac, and the top of each white konjac is covered with a 3-5cm sunshade.

2. The method for controlling major diseases and pests of macadamia nuts in an interplanting mode according to claim 1, wherein the white konjac seeds are soaked with gibberellin and 6-BA, then spread out for sun-drying for one day, and then sprayed with a mixture of plant ash and matrine metal complex according to a proportion, and then sowed.

3. The method for controlling major macadimia nut pests according to the interplanting mode of claim 2, wherein the ratio of the plant ash to the matrine metal complex is 100: 1-3.

4. The method for controlling major macadimia nut pests according to an interplanting mode of claim 2 or 3, wherein the matrine metal complex is zinc or iron.

5. The method for preventing and controlling major diseases and pests of macadamia nuts in an interplanting mode according to claim 4, wherein the preparation method of the matrine metal complex is that matrine, zinc chloride/iron and hydrochloric acid are dissolved in absolute ethyl alcohol according to the mass ratio of 2-3:1-2:0.4-0.5, and the absolute ethyl alcohol is volatilized to obtain light yellow crystals.

6. The method for controlling major macadimia nut pests and diseases in an interplanting mode according to claim 1, wherein the sunshade is straw, rice straw or pine needles.

7. The method for controlling major macadimia nut pests according to the interplanting mode of claim 3, wherein the ratio of plant ash to matrine metal complex is 100: 3.

Technical Field

The invention belongs to the field of biological control of diseases and pests, and particularly relates to a method for controlling major diseases and pests of macadimia nuts in an interplanting mode.

Background

Macadamia nutMacadamia spp.(F, Muell), also known as Kunstland nut, Macadamia nut and Hawaii fruit, is a perennial evergreen fruit tree of the genus Macadamia of the Pitaya ophthalmological Proteaceae Macadamia nut, and is originally produced in subtropical rainforest regions along the east of Kunstland and the northeast of New south Wales of Australian. At present, macadamia nuts are planted in more than 20 countries and regions in the world, the Chinese planting area is at the top of the world, the Yunnan province relies on the unique natural resource advantage, the development of the plateau characteristic woody oil industry is accelerated, the macadamia nut planting area is at the top of the whole country, and according to statistics, the planting area of the macadamia nuts in the Yunnan province reaches 10.67 kilohm in 2015 year2The planting base is newly increased by 2.67 kilohm every year2With the rapid macadamia nut industryThe rapid development, the continuous expansion of the planting area, the change of the surrounding ecological environment and the climate condition, the variety and the degree of the plant diseases and insect pests in the macadimia nut planting area are diversified, in the process of preventing and controlling the diseases and the pests, the main preventing and controlling method still uses pesticide for preventing and controlling, and pesticide residues exist inevitably, so that the suitable biological preventing and controlling method is very urgent to find.

Disclosure of Invention

The invention mainly aims to provide a method for preventing and controlling major macadimia nut diseases and insect pests in an interplanting mode, so as to reduce the occurrence of the macadimia nut diseases and insect pests and the use of pesticides.

In order to achieve the above purpose, the invention provides the following technical scheme:

a method for preventing and controlling major diseases and pests of macadamia nuts in an interplanting mode is characterized in that white konjak is interplanted in a 5-year-old macadamia nut forest, and comprises the following steps:

s1: selecting not less than 500g of white konjac seeds, wherein the corms are required to have rough surface, deep color and plump bud bodies;

s2: soaking the selected white konjac seeds in gibberellin and 6-BA for 2 hours, wherein the concentration of the gibberellin is 100mg/L, and the concentration of the 6-BA is 150 mg/L;

s3: the planting density of the macadimia nuts is 25-35 seeds per mu, the seeding density of the white konjac is 200-300 seeds per mu, the soaked white konjac seeds are respectively sown under the macadimia nut forest in the last ten days of 1 month and the middle ten days of 3 months, 50% of the seeds are respectively sown under the macadimia nut forest, the distance between the white konjac and the macadimia nut trunk is not less than 60cm, the plant spacing between the white konjac is not less than 50cm, the cultivation depth is 15-20cm, no less than 200g of farmyard manure is applied to the bottom of each white konjac, and the top of each white konjac is covered with a 3-5cm sunshade.

The Fibract inflorescence is the peculiar inflorescence structure of the Araceae plant, and the inflorescence structure and the pollinated beetle have high adaptive evolutionary relationship, firstly, a closed chamber is usually formed during blooming to provide a concealed place for the beetle to take food, call for spouse, mate and the like, secondly, the place where the beetle lives is simulated through visual and olfactory signals to attract the beetle, the visual signals are usually deep purple red and brown yellow which simulate dead spots, feces and the like, the olfactory signals are mainly used for releasing foul smell of compounds of sulfide, indole, phenol, terpene and the like which are main chemical components to simulate rotten bodies, excreta, fungi and the like, in addition, the stamens and the stamens in the Araceae plant inflorescences have the characteristic of being different maturity, the beetle is attracted before the pistils are matured, then the Fibract beetles are designed into various traps or are attracted by food for about 24 hours through the structure of the inflorescence, until the stamen is mature, the beetles carrying pollen cannot leave.

The inflorescence of the amorphophallus konjac belongs to the bud inflorescence of the spathe, but the bud color of the amorphophallus konjac is yellow green or yellow, and is greatly different from the bud color of inflorescences of other plants in the amorphophallus. A large amount of cryptopterus affinis are gathered in inflorescences when the konjac is blossoming, the konjac is heterotrophy and has prematurity in female and male parts, strong odor is emitted from an accessory during the female flowering period, gas chromatography-mass spectrometry (GC-MS) analysis shows that the odor components of flowers mainly comprise dimethyl disulfide and dimethyl trisulfide, the above information is integrated, namely, the konjac attracts beetles and gathers cryptoptera, and emits odor during the whole flowering period, the beetles and the cryptoptera are both beneficial pests in macadamia nut forests, the compounds such as the dimethyl disulfide and the dimethyl trisulfide are usually intermediates of pesticides, the growing environment of the konjac has homology with that of the macadamia nuts, and therefore, the idea is provided for interplanting the konjac and the macadamia nuts to control diseases and insect pests of the macadamia nuts.

The invention firstly selects white konjak seeds of not less than 500g, the white konjak seeds are soaked by gibberellin and 6-BA, the preferred concentration of the gibberellin is 100mg/L, the concentration of the 6-BA is 150mg/L, then, sowing in late 1 and middle 3 months, wherein the seeds after soaking can differentiate 2-3 buds on average after planting, the flowering period of the macadimia nuts is 3-4 months, the time for planting the konjac is 3-4 months generally, the flowering period is 7-8 months, in the macadimia nut pest control process, the most important control time is the fruit growth period after the flowering phase, the method has great influence on the shape and quality of the fruit, the planting time is prolonged to 1 month, and the white konjak can continuously bloom in the fruit stage of the macadamia nut through germplasm treatment and maintain a long-time flowering period.

The planting density of the macadimia nuts and the white konjac seeds has great influence on the result of pest control, the density is not enough, the concentration of the malodorous chemical components emitted by the spathe inflorescences in the forest cannot achieve the purpose of repelling pests, and the density is too high, so that the growth of the nuts in the forest is influenced, and the cost is quite high.

Under the conception of the technical scheme of the invention, in the planting process, the soft rot occurrence probability is higher in the konjak planting process, the seeds used in the invention are seeds with the weight of more than 500g, the seeds are selected and soaked to cause damage to the germplasm to a certain extent, the soft rot occurrence probability is greatly improved in the planting process, the conventional planting time is 3-4 months, and the air temperature is warmed up, while the first planting time is 1 month, the average air temperature is lower at the moment, the influence on the freeze injury damage of the seeds is aggravated, the survival rate is lower during the seed testing period, so that the cost is increased, a better method for increasing the seedling survival rate is found and becomes a difficulty, the white konjak seeds are soaked by gibberellin and 6-BA, are spread out for one day, and then are prepared into a mixture by using plant ash and matrine metal complex according to a proportion and are sprayed on the white konjak seeds, preferably, the ratio of the plant ash to the matrine metal complex is 100:1-3, the preferable metal is zinc or iron, then sowing is carried out, and through the test, the survival rate of the white konjac seeds after cultivation can reach more than 90%.

The invention adopts matrine metal complex as active ingredient, in order to achieve the effect of preventing and treating konjak diseases, laboratory tests show that the survival rate of the konjak can reach more than 90%, the preparation method of the matrine metal complex comprises the steps of taking matrine, zinc chloride/iron and hydrochloric acid according to the mass ratio of 2-3:1-2:0.4-0.5, dissolving the matrine, zinc chloride/iron and hydrochloric acid in absolute ethyl alcohol, and volatilizing the absolute ethyl alcohol to obtain light yellow crystals, but the konjak seeds used in the invention have larger volume, if liquid ingredients are sprayed during field operation, certain mechanical damage can be avoided again due to the problem of sufficient spraying, and the moisture can accelerate the abrasion of seed coats and is not beneficial to sowing, therefore, the matrine metal complex is crushed into particles (less than 500 um) according to the mass ratio of 100:3, after the mixture is mixed with the konjac seeds, field experiments show that the survival rate is improved to more than 90% after the operation of the step, guess that after the plant ash is used, the plant ash not only provides certain nutrient elements, but also plays a role in subsidiary sterilization, has small granularity, can be adsorbed on the wound surface of the konjac, achieves the effect of heat preservation and sterilization, and greatly improves the survival rate of the konjac by combining the condition of the matrine metal complex.

According to the method, the white konjak and the macadimia nuts are interplanted, the flowering phase and the planting density are controlled, and the white konjak maintains a certain flowering phase in the fruit phase of the macadimia nuts, so that the effect of preventing and controlling the diseases and insect pests of the macadimia nuts is achieved through the physiological characteristics of the white konjak, pesticide residues are not caused like the traditional pesticide prevention and control, and the excellent rate of the macadimia nuts is over 80%.

According to the method, 2-3 buds are averagely differentiated from the konjac by soaking seeds with gibberellin and 6-BA, so that the inflorescence of the spathe is increased, and the concentration of the smell emitted by the inflorescence in the environment is increased.

According to the invention, the plant ash and the matrine metal complex are used for treating the konjac seeds, so that the survival rate of the konjac is improved, the morbidity probability of soft rot is reduced, and the survival rate after planting reaches 90%.

Detailed Description

The invention is further illustrated by the following specific examples, wherein the following survey method is used to survey in mid-4 months after planting: and (3) carrying out net sweeping on the Australian nut, surveying for 1 time every 10 days, continuously surveying for 5 times, sweeping for 20 times each time, drawing an insect catching net (with the mesh size of 0.125 mm), recording the types of the pests, the natural enemies and the occurrence condition of the pests, taking the samples back to a laboratory for treatment, setting a control group, and calculating the types and the number of the main pests.

Example 1

The planting test field is Lincang Yongde, and the specific control method is as follows:

a method for preventing and controlling major diseases and pests of macadamia nut in interplanting mode is characterized in that white konjak is interplanted in a 5-year-old macadamia nut forest,

s1: selecting about 500g of white konjac seeds, wherein the corms are required to have rough surface, deep color and plump bud bodies;

s2: soaking the selected white konjac seeds in gibberellin and 6-BA for 2 hours, wherein the concentration of the gibberellin is 100mg/L, and the concentration of the 6-BA is 150 mg/L;

s3: soaking seeds of the white konjac in gibberellin and 6-BA, spreading the seeds out for airing for one day, preparing a mixture of plant ash and a matrine zinc metal complex according to a ratio of 100:3, spraying the mixture on the seeds of the white konjac, and then sowing;

s3: the planting density of the macadimia nuts is 30 per mu, the seeding density of the white konjac is 300 per mu, the soaked white konjac seeds are respectively sown under the macadimia nut forest in 1 middle ten days and 3 middle ten days, 50% of the seeds are respectively sown under the macadimia nut forest, the distance between the white konjac and the macadimia nut trunk is not less than 60cm, the plant spacing between the white konjac is not less than 50cm, the cultivation depth is 15-20cm, no less than 200g of farmyard manure is applied to the bottom of each white konjac, and the top of each white konjac is covered with 3-5cm straws or pine needles.

The conventional statistical investigation shows that the number of harmful insects in the Yunnan Australian nut garden exceeds 18, and the harmful insects span 9-mesh 13-familyCryptophlebia illepida(Butler), Dolphite Green bugNezara viridula(Linnaeus), moth-eating fruit borerCryptophlebia ombrodelta(Lower), apple shoot bark beetleHypothenemus obscurus

The total number of four pillar fruit pests obtained comprehensively in five times of records is 32, 45, 36, 51 and 26 respectively, the records of no-planting blocks are 61, 105, 80, 64 and 85, as can be seen from data, in the fruit stage of macadamia nut, the number of the pillar fruit pests in the environment is obviously reduced after the white konjac is planted, compared with the land blocks without the white konjac, the pillar fruit pests in the orchard after the white konjac is planted are reduced by 51 percent through the average value analysis and comparison of the data, the quality of the fruits is greatly improved, the fruits in the mature period are further analyzed, 10 fruit trees are randomly selected, 100 fruits are selected, the appearance of the fruits is checked, the single fruit is weighed, the fruit shape is complete, the insect-free high-quality fruit rate reaches 86 percent, and the high-quality fruit rate in the environment without the white konjac is only 57 percent.

Because the konjac is easy to have soft rot and the konjac of a large variety is more easy to have mechanical damage in the cultivation process, so that the infection of plant diseases and insect pests is aggravated and the survival rate is reduced, in order to further control the cost and enable the konjac flowers in the fruit stage of the Australian nuts to reach the effective amount of the environment, hormone stimulation is adopted to lead the white konjac to differentiate a plurality of flower buds, the gibberellin is adopted in the conventional exploration on the florescence and fertility of the konjac by the external GA3 of the Li Yongjun and the like, so that the bud differentiation can be stimulated, the induction rate is increased along with the increase of the treatment concentration, the main biological characteristics of the induced flower are consistent with those of the normal flower, but the flowering period is delayed after the induction, the method is not suitable for being directly used in an Australian orchard, in order to lead the flowering period of the konjac, the importance of the hormone on the plant is considered, the flowering condition under the influence of different hormones is designed, and the gibberellin is used at 100mg/L, respectively collocating with NAA, ZT and 6-BA according to concentration gradient of 50mg/L, 100mg/L and 150mg/L, soaking in clear water as a contrast, soaking white konjak seeds (500 g +/-30 g) for 2h, airing in the sun for one day, respectively processing 100 seeds, sowing in the field in 1 month late ten days, wherein the average environmental temperature is 15 ℃ during sowing, counting the flowering induction rate (the non-survival plants are not counted in flowering time) at the bottom of 4 months, repeatedly setting 3 groups for each condition, and calculating the average value to obtain the average induction rate: the clear water is 10.5 percent, the inductivity of the combination of gibberellin and NAA is 21.3 percent, 34 percent and 43.5 percent, the inductivity of the combination of gibberellin and ZT is 42.6 percent, 30.1 percent and 51.3 percent, the inductivity of the combination of gibberellin and 6-BA is 42.4 percent, 48.2 percent and 65.6 percent, and the data shows that the flower formation inductivity of the white konjac seeds treated by using the hormone is improved by comparing with that of the clear water, and particularly, the effect of the white konjac seeds treated by the combination of gibberellin and cytokinin 6-BA is the best.

In the actual field planting process, the death rate of the konjak is found to be high, in order to reduce the death rate after planting, 100mg/L gibberellin and 6-BA150mg/L are adopted for soaking for two hours, then the konjak is aired for one day, plant ash and matrine metal complex are prepared into a mixture according to the proportion of 100:1-3 and sprayed on the konjak seeds, the metal is zinc, the matrine metal complex is prepared by taking matrine, zinc chloride and hydrochloric acid according to the mass ratio of 2.5:2: 0.5, dissolving the mixture in absolute ethyl alcohol, obtaining light yellow crystals after the absolute ethyl alcohol is volatilized, grinding the mixture to ensure that the granularity is less than 500um, mixing the mixture according to the mass ratio of 100:1, 100:2 and 100:3 respectively, then spraying the mixed powder on the surface of the konjak seeds, after 100 seeds are treated according to each proportion, 3 groups are repeatedly arranged according to each proportion, and the seeds are sowed in the field 1 month, counting the induction rate of flowering (the non-flowering plants are counted) at the end of 4 months, respectively accounting the induction rate of 54.1%, 67.3% and 89% of the treated white konjac, respectively accounting the survival rate of the white konjac at 70.1%, 85% and 92.6%, and according to the statistical data, after retreatment, using plant ash and matrine metal complex plays a certain role in reducing the death rate of konjac planting, and considering the use cost problem, the proportion of 100:3 is optimally selected for spraying.

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